GS-TOF-MS analysis of plasma and saliva collected from SSC vs benign control cases. (a) Metabolites grouped by chemical class were identified from either plasma (top) or saliva (bottom). Color scale represents fold change for cancer/benign, with red representing a higher prevalence in cancer biofluids and blue a higher prevalence in the controls. (b) PLS-DA of the pre-labeled cancer and control samples for plasma and saliva reveals excellent discrimination with only a single sample misclassified in each biofluid. (c) ROC curves enable calculation of AUC to be 95.9% and 92.4% for plasma and saliva, respectively.
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