This is the development version of HSMMSingleCell; for the stable release version, see HSMMSingleCell.
Single-cell RNA-Seq for differentiating human skeletal muscle myoblasts (HSMM)Bioconductor version: Development (3.22)
Skeletal myoblasts undergo a well-characterized sequence of morphological and transcriptional changes during differentiation. In this experiment, primary human skeletal muscle myoblasts (HSMM) were expanded under high mitogen conditions (GM) and then differentiated by switching to low-mitogen media (DM). RNA-Seq libraries were sequenced from each of several hundred cells taken over a time-course of serum-induced differentiation. Between 49 and 77 cells were captured at each of four time points (0, 24, 48, 72 hours) following serum switch using the Fluidigm C1 microfluidic system. RNA from each cell was isolated and used to construct mRNA-Seq libraries, which were then sequenced to a depth of ~4 million reads per library, resulting in a complete gene expression profile for each cell.
Author: Cole Trapnell
Maintainer: Cole Trapnell <cole at broadinstitute.org>
Citation (from within R, entercitation("HSMMSingleCell")): Installation
To install this package, start R (version "4.5") and enter:
if (!require("BiocManager", quietly = TRUE))
install.packages("BiocManager")
# The following initializes usage of Bioc devel
BiocManager::install(version='devel')
BiocManager::install("HSMMSingleCell")
For older versions of R, please refer to the appropriate Bioconductor release.
Documentation Details biocViews ExperimentData, RNASeqData Version 1.29.0 License Artistic-2.0 Depends R (>= 2.10) Imports System Requirements URL See More Suggests Linking To Enhances Depends On Me Imports Me monocle Suggests Me Links To Me Build Report Build Report Package ArchivesFollow Installation instructions to use this package in your R session.
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