Some links related to the case study:
The following command was used to generate the config:
nextflow pull vib-singlecell-nf/vsn-pipelines nextflow config vib-singlecell-nf/vsn-pipelines \ -profile sra,cellranger,pcacv,bbknn,dm6,scenic,scenic_use_cistarget_motifs,scenic_use_cistarget_tracks,singularity \ > nextflow.config
The generated config is available at the vsn-pipelines-examples
GitHub repository: kurmangaliyevyz_2019/10x_bbknn_scenic.config. You should provide the lines commented with âTO EDITâ with the correct information.
To start the pipeline, run the following command:
nextflow \ -C nextflow.config \ run vib-singlecell-nf/vsn-pipelines \ -entry sra_cellranger_bbknn_scenic
The resulting loom file is available at kurmangaliyevyz_2019_bbknn_scenic.loom, and is ready to be explored in SCope.
Harmony and SCENIC (append mode)¶nextflow config \ vib-singlecell-nf/vsn-pipelines \ -profile tenx,pcacv,harmony,scenic_append_only,singularity \ > nextflow.config
The generated config is available at the vsn-pipelines
GitHub repository: kurmangaliyevyz_2019/10x_harmony_scenic_append_only.config. You should update The lines commented with âTO EDITâ with the correct information.
To start the pipeline, run the following command:
nextflow \ -C nextflow.config \ run vib-singlecell-nf/vsn-pipelines \ -entry harmony_scenic -resume
The resulting loom file is available at kurmangaliyevyz_2019/harmony_scenic_append_only and is ready to be explored in SCope.
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