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Bioaccumulation of paralytic shellfish poisoning (PSP) toxins from the cyanobacterium Anabaena circinalis by the freshwater mussel Alathyria condola

Abstract

The Australian fresh-water mussel Alathyria condola accumulated high levels of paralytic shellfish poisoning (PSP) toxins when fed the neurotoxic cyanobacterium Anabaena circinalis, shown recently to contain high concentrations of C-toxins and gonyautoxins. Significant accumulation (>; 80 μg/100 g of mussel flesh) was detected following 2–3 days exposure to water containing 2 × 10 5 cells/ml A. circinalis. Only trace accumulation of PSP toxins was demonstrated over long-term (5 week) exposure at low concentration ( c. 10 4 cells/ml). The relative abundance of C-toxins, gonyautoxins and saxitoxins in mussels generally matched the toxin profiles of the dietary A. circinalis, although there were differences in the GTX2/3 and C1/2 ratios with time, and an increase in abundance of decarbamoylgonyautoxins. Analysis of mussel tissues after 7 days, exposure to A. circinalis revealed that 96% of the toxins were accumulated in the viscera. As in marine waters, the bioaccumulation of PSP toxins in freshwater mussels may pose a health risk to humans and animals, especially in areas where seasonally decreasing water levels expose mussel beds to surface scums of toxic cyanobacteria.


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