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Showing content from https://pubmed.ncbi.nlm.nih.gov/8588914/ below:

Genetic transformation of nematodes using arrays of micromechanical piercing structures

Affiliations

• ¹ Entomology Department, Cook College, Rutgers University, New Brunswick, NJ 08903, USA.

• PMID: 8588914

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S Hashmi et al. Biotechniques. 1995 Nov.

• ¹ Entomology Department, Cook College, Rutgers University, New Brunswick, NJ 08903, USA.

• PMID: 8588914

Item in Clipboard

Foreign genes were expressed in the nematode Heterorhabditis bacteriophora using a new micro-mechanical device to inject DNA-coated microprobe through the nematode cuticle. After introduction of a plasmid in which a Caenorhabditis elegans 16-kDa heat shock promoter was fused to Escherichia coli, a beta-galactosidase gene was expressed in the progeny of the injected worms. The tip of the microprobe penetrated through the nematode cuticle without causing injury to the nematode, and about 8% of the total progeny tested expressed the foreign gene. We describe and validate a new and efficient genetic transformation system for nematodes. The method is quick and easy to use.

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• Microneedle-mediated drug delivery for cutaneous diseases.

  Chen J, Ren H, Zhou P, Zheng S, Du B, Liu X, Xiao F. Chen J, et al. Front Bioeng Biotechnol. 2022 Oct 17;10:1032041. doi: 10.3389/fbioe.2022.1032041. eCollection 2022. Front Bioeng Biotechnol. 2022. PMID: 36324904 Free PMC article. Review.

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