RetroSearch Browse

Home - News ( United States | United Kingdom | Italy | Germany ) - Football scores

Showing content from https://pubmed.ncbi.nlm.nih.gov/34976577/ below:

Design and evaluation of Raman reporters for the Raman-silent region

doi: 10.7150/ntno.58965. eCollection 2022. Design and evaluation of Raman reporters for the Raman-silent region

Affiliations

¹ Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY, USA.
² Molecular Imaging Program at Stanford University (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
³ Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA.
⁴ Department of Radiology, Perelman School of Medicine, University of Pennsylvania, PA, USA.
⁵ Department of Bioengineering, Stanford University School of Medicine, Stanford, CA, USA.
⁶ Department of Material Science & Engineering, Stanford University School of Engineering, Stanford, CA, USA.

PMID: 34976577
PMCID: PMC8671958
DOI: 10.7150/ntno.58965

Item in Clipboard

Design and evaluation of Raman reporters for the Raman-silent region

Konstantinos Plakas et al. Nanotheranostics. 2022.

doi: 10.7150/ntno.58965. eCollection 2022. Affiliations

¹ Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY, USA.
² Molecular Imaging Program at Stanford University (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
³ Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA.
⁴ Department of Radiology, Perelman School of Medicine, University of Pennsylvania, PA, USA.
⁵ Department of Bioengineering, Stanford University School of Medicine, Stanford, CA, USA.
⁶ Department of Material Science & Engineering, Stanford University School of Engineering, Stanford, CA, USA.

PMID: 34976577
PMCID: PMC8671958
DOI: 10.7150/ntno.58965

Item in Clipboard

Abstract

Rationale: Surface enhanced Raman scattering (SERS) is proving to be a useful tool for biomedical imaging. However, this imaging technique can suffer from poor signal-to-noise ratio, as the complexity of biological tissues can lead to overlapping of Raman bands from tissues and the Raman reporter molecule utilized. Methods: Herein we describe the synthesis of triple bond containing Raman reporters that scatter light in the biological silent window, between 1750 cm^-1 and 2750 cm^-1. Results: Our SERS nanoprobes are comprised of uniquely designed Raman reporters containing either alkyne- or cyano-functional groups, enabling them to be readily distinguished from background biological tissue. Conclusion: We identify promising candidates that eventually can be moved forward as Raman reporters in SERS nanoparticles for highly specific contrast-enhanced Raman-based disease or analyte detection in biological applications.

Keywords: Nanotag; Raman-silent region; surface-enhanced Raman scattering; triple bonds.

PubMed Disclaimer

Conflict of interest statement

Competing Interests: Stefan Harmsen is listed as inventor on several patents related to the SERS technology used in this work. Sanjiv Sam Gambhir holds the original patent on the use of SERS in living subjects.

Figures
Scheme 1

General Synthesis of Raman Reporters.

Scheme 1

General Synthesis of Raman Reporters.

Scheme 1

General Synthesis of Raman Reporters.

Figure 1

Surface-enhanced Raman scattering spectra of…

Figure 1

Surface-enhanced Raman scattering spectra of 4-ethynylphenyl substituted pyrylium dyes ( 1-4d ). (a)…

Figure 1

Surface-enhanced Raman scattering spectra of 4-ethynylphenyl substituted pyrylium dyes (1-4d). (a) SERS spectra of pyrylium dyes 1-3c after 638-nm excitation. (b) SERS spectra of pyrylium dyes 4a-d after 638-nm excitation. (c) SERS spectra of pyrylium dyes 1-3c after 785-nm excitation. d) SERS spectra of pyrylium dyes 4a-d after 785-nm excitation.

Figure 2

SERS spectra of dyes 5a-d…

Figure 2

SERS spectra of dyes 5a-d upon 638-nm ( a ) and 785-nm (…

Figure 2

SERS spectra of dyes 5a-d upon 638-nm (a) and 785-nm (b) laser excitation.

Figure 3

SERS spectra of cyano-substituted thio-…

Figure 3

SERS spectra of cyano-substituted thio- ( 6a-8a ) and selenoxanthylium ( 6b-8b )…

Figure 3

SERS spectra of cyano-substituted thio- (6a-8a) and selenoxanthylium (6b-8b) dyes upon 638-nm (a) and 785-nm (b) laser excitation

Figure 4

SERS spectra of dye 9…

Figure 4

SERS spectra of dye 9 upon 638-nm (a) and 785-nm (b) laser excitation.

Figure 4

SERS spectra of dye 9 upon 638-nm (a) and 785-nm (b) laser excitation.

Figure 5

Raman bands in the Raman-silent…

Figure 5

Raman bands in the Raman-silent region . a, Structures of the selected resonant…

Figure 5

Raman bands in the Raman-silent region. a, Structures of the selected resonant Raman reporters with the most intense Raman bands in the silent region. b, Composite of measured Raman intensity, and c, relative Raman intensity upon 638-nm laser excitation. d, Composite of measured Raman intensity, and e, Relative Raman intensity upon 785-nm laser excitation.