Figure 1
SERS spectra of the four…
Figure 1
SERS spectra of the four main DNA nucleotides, (a) polyadenosine, Poly A, (b)…
Figure 1SERS spectra of the four main DNA nucleotides, (a) polyadenosine, Poly A, (b) polythymidine, Poly T (c) polycytosine, Poly C and (d) guanosine dinucleotide, G dimer, (e) the SERS spectrum of a ss-DNA sequence: CTT–TTT–CCT–GCA–TCC–TGT–CTG–GAA–G. Reprinted from reference [41] with permission from the Royal Society of Chemistry. Copyright 2017 RSC.
Figure 2
Scheme of label-free sensor based…
Figure 2
Scheme of label-free sensor based on spectral changes induced by changes in the…
Figure 2Scheme of label-free sensor based on spectral changes induced by changes in the orientation of the DNA strand on the surface after hybridization [10]; orange is capture DNA immobilized on the metal surface and red is the target DNA.
Figure 3
Scheme of label-free sensor: green…
Figure 3
Scheme of label-free sensor: green is capture DNA immobilized on nanoparticles, red is…
Figure 3Scheme of label-free sensor: green is capture DNA immobilized on nanoparticles, red is the target DNA (for example, mutated DNA from a clinical sample), and violet is complementary DNA immobilized on nanoparticles.
Figure 4
SERS spectra of ssDNA (blue…
Figure 4
SERS spectra of ssDNA (blue lines) after interaction with DNA isolated from FFPE…
Figure 4SERS spectra of ssDNA (blue lines) after interaction with DNA isolated from FFPE tumor tissue, with detected mutation (A), DNA isolated from plasma patients without detected mutation (B) and non–complementary synthetic DNA. Reprinted from reference [10] with permission from Elsevier. Copyright 2019 Elsevier.
Figure 5
A scheme of SWNT-based SERS…
Figure 5
A scheme of SWNT-based SERS assay coupling with RNase HII-assisted amplification for highly…
Figure 5A scheme of SWNT-based SERS assay coupling with RNase HII-assisted amplification for highly sensitive detection of ctDNA in human blood [61]; blue is hairpin capture DNA immobilized on nanoparticles, red is the T-rich DNA, green is circulating tumor DNA, grey is single-walled carbon nanotube, and light blue are copper nanoparticles.
Figure 6
Scheme of sandwich type sensor:…
Figure 6
Scheme of sandwich type sensor: green is capture DNA immobilized on the metal…
Figure 6Scheme of sandwich type sensor: green is capture DNA immobilized on the metal surface, red is the target DNA (for example, mutated DNA from a clinical sample), and violet is complementary reporter DNA labelled with an RR.
Figure 7
A prototype of sandwich type…
Figure 7
A prototype of sandwich type sensor consisting of two DNA strands by Vo-Dinh…
Figure 7A prototype of sandwich type sensor consisting of two DNA strands by Vo-Dinh and co-workers [66]; green is capture DNA immobilized on the metal surface and violet is the target DNA labelled with an RR.
Figure 8
Different modifications of sandwich type…
Figure 8
Different modifications of sandwich type of sensors based on: ( A ) the…
Figure 8Different modifications of sandwich type of sensors based on: (A) the gold nanoparticle-decorated chemical vapor deposition (CVD)-growth graphene [77], (B) the Au particle-on-wire system [81]; (C) the core-satellite Au nanoparticles structure [78]; green and grey are capture DNA immobilized on the metal surface, red and light blue are the target DNA (for example, mutated DNA from a clinical sample), and violet and brown are complementary reporter DNA labelled with an RR.
Figure 9
( A ) General scheme…
Figure 9
( A ) General scheme of sandwich sensor with RR located on nanoparticles;…
Figure 9(A) General scheme of sandwich sensor with RR located on nanoparticles; (B) Scheme of DNA conjugates with dye-coded nanoparticles [89]; (C) Scheme of DNA conjugates with dye-coded and magnetic nanoparticles [44]; green and violet are capture DNA immobilized on magnetic nanoparticles or nanoparticles labelled with RR and red is the target DNA (for example, mutated DNA from a clinical sample).
Figure 10
Schematic illustration of the Exo…
Figure 10
Schematic illustration of the Exo III-fueled target recycling-programmed cascade reactions integrated with SERS…
Figure 10Schematic illustration of the Exo III-fueled target recycling-programmed cascade reactions integrated with SERS Technology for DNA Detection [102]; blue is hairpin probe DNA, red is the target DNA from clinical sample, black is capture DNA immobilized on the metal surface, green and brown are hairpin DNA labelled with RR.
Figure 11
General scheme of hairpin on-off…
Figure 11
General scheme of hairpin on-off sensor: blue is hairpin capture DNA immobilized on…
Figure 11General scheme of hairpin on-off sensor: blue is hairpin capture DNA immobilized on nanoparticles labelled with RR, red is the target DNA (for example, mutated DNA from a clinical sample).
Figure 12
Example of hairpin on–off sensor…
Figure 12
Example of hairpin on–off sensor with RR and fluorescent dye (FAM). Blue is…
Figure 12Example of hairpin on–off sensor with RR and fluorescent dye (FAM). Blue is hairpin capture DNA immobilized on nanoparticles labelled with RR and FAM, red is the target DNA (for example, mutated DNA from a clinical sample) [113].
Figure 13
General scheme of hairpin off–on…
Figure 13
General scheme of hairpin off–on sensor: blue is capture DNA immobilized on metal…
Figure 13General scheme of hairpin off–on sensor: blue is capture DNA immobilized on metal surface labelled with RR, red is a “placeholder” DNA, and green is the target DNA (for example, mutated DNA from a clinical sample).
Figure 14
Recorded SERS spectra of: blank…
Figure 14
Recorded SERS spectra of: blank samples (blue line), non-complementary ssDNA (red line) and…
Figure 14Recorded SERS spectra of: blank samples (blue line), non-complementary ssDNA (red line) and complementary target ssDNA samples (green line). Reprinted from reference [116] with permission from Royal Society of Chemistry. Copyright 2014 RSC.
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