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Showing content from https://pubmed.ncbi.nlm.nih.gov/30530303/ below:

Achieving Automated Organelle Biopsy on Small Single Cells Using a Cell Surgery Robotic System

• PMID: 30530303
• DOI: 10.1109/TBME.2018.2885772

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Adnan Shakoor et al. IEEE Trans Biomed Eng. 2019 Aug.

• PMID: 30530303
• DOI: 10.1109/TBME.2018.2885772

Item in Clipboard

Single cell surgery such as manipulation or removal of subcellular components or/and organelles from single cells is increasingly used for the study of diseases and their causes in precision medicine. This paper presents a robotic surgery system to achieve automated organelle biopsy of single cells with dimensions of less than 20 μm in diameter. The complexity of spatial detection of the organelle position is reduced by patterning the cells using a microfluidic chip device. A sliding mode nonlinear controller is developed to enable extraction of organelles, such as the mitochondria and the nucleus, from single cells with high precision. An image processing algorithm is also developed to automatically detect the position of the desired organelle. The effectiveness of the proposed robotic surgery system is demonstrated experimentally with automated extraction of mitochondria and nucleus from human acute promyelocytic leukemia cells and human fibroblast cells. Extraction is followed by biological tests to indicate the functionality of biopsied mitochondria as well as the cell viability after removal of mitochondria. The results presented here have revealed that the proposed approach of automated organelle biopsy on single small cells is feasible.

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• Advanced tools and methods for single-cell surgery.

  Shakoor A, Gao W, Zhao L, Jiang Z, Sun D. Shakoor A, et al. Microsyst Nanoeng. 2022 Apr 29;8:47. doi: 10.1038/s41378-022-00376-0. eCollection 2022. Microsyst Nanoeng. 2022. PMID: 35502330 Free PMC article. Review.

• Visually precise, low-damage, single-cell spatial manipulation with single-pixel resolution.

  Zhang Q, Shao Y, Li B, Wu Y, Dong J, Zhang D, Wang Y, Yan Y, Wang X, Pu Q, Guo G. Zhang Q, et al. Chem Sci. 2021 Feb 2;12(11):4111-4118. doi: 10.1039/d0sc05534d. Chem Sci. 2021. PMID: 34163682 Free PMC article.

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