Receptor analysis (A) and susceptibility test (B) results for bat SL-CoV WIV16. (A) HeLa cells with and without the expression of ACE2. ACE2 expression was detected with goat anti-human ACE2 antibody, followed by fluorescein isothiocyanate (FITC)-conjugated donkey anti-goat IgG. Virus replication was detected with rabbit antibody against the SL-CoV Rp3 nucleocapsid protein, followed by cyanine 3 (Cy3)-conjugated mouse anti-rabbit IgG. Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole). The columns (from left to right) show staining of nuclei (blue), ACE2 expression (green), virus replication (red), and all three (merged triple-stain images). b, bat; c, civet; h, human. (B) Virus infection in A549, LLC-MK2, RSKT, PK15, H292, and Vero-E6 cells. The columns (from left to right) show staining of nuclei (blue), virus replication (red), and both nuclei and virus replication (merged double-stain images). A549 and H292, human lung cells; LLC-MK2, macaque kidney cells; RSKT, Chinese horseshoe bat kidney cells; PK15, pig kidney cells; VeroE6, African green monkey kidney cells.
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