Home - News ( United States | United Kingdom | Italy | Germany ) - Football scores

Showing content from https://pubmed.ncbi.nlm.nih.gov/23072258/ below:

Enzyme-linked immunosorbent assay for p16(INK4a) - a new triage test for the detection of cervical intraepithelial neoplasia?

Affiliations

• ¹ Department of Gynaecology and Obstetrics, Hannover Medical School, Hannover, Germany. jentschke.matthias@mh-hannover.de

• PMID: 23072258
• DOI: 10.1111/aogs.12032

Item in Clipboard

Matthias Jentschke et al. Acta Obstet Gynecol Scand. 2013 Feb.

• ¹ Department of Gynaecology and Obstetrics, Hannover Medical School, Hannover, Germany. jentschke.matthias@mh-hannover.de

• PMID: 23072258
• DOI: 10.1111/aogs.12032

Item in Clipboard

Objective: To evaluate enzyme-linked immunosorbent assay (ELISA) for cyclin-dependent kinase inhibitor 2A protein (p16(INK4a) ) on self-collected cervicovaginal lavage samples as an additional triage test to identify women with high-grade cervical intraepithelial neoplasia (CIN).

Design: Retrospective feasibility, sensitivity and specificity study.

Setting: University Medical School, Germany.

Sample: One hundred and fifty-two patients from the colposcopy clinic were included.

Methods: All women used a cervico-vaginal lavage device (Delphi Screener) for self-sampling and had gynecological examinations with Pap smears, cervical smears in ThinPrep PreservCyt solution and Cervatec medium for human papillomavirus (HPV) testing (Qiagen Hybrid Capture 2) and colposcopic examinations with biopsies if abnormalities were detected (72 women; 51%). All cytological samples were examined by p16(INK4a) ELISA.

Main outcome measures: Sensitivity and specificity of p16(INK4a) ELISA for high-grade CIN.

Results: Complete data were available for 140 women. Among these, 62 women (46%) presented with an atypical Pap smear and 65 (46.4%) were high-risk HPV positive in the reference smear sample. Seventeen women (12%) had CIN 3+. Twenty-seven (19%) physician-collected samples were p16(INK4a) ELISA positive. In contrast, p16(INK4a) ELISA turned out to be positive in only one (1%) vaginal lavage sample.

Conclusions: Our study shows that self-sampling with cervicovaginal lavage followed by p16(INK4a) ELISA is not suitable for the detection of high-grade CIN.

© 2012 The Authors Acta Obstetricia et Gynecologica Scandinavica© 2012 Nordic Federation of Societies of Obstetrics and Gynecology.

PubMed Disclaimer

• [Clinical value of p16^INK4a immunocytochemistry in cervical cancer screening].

  Song FB, Du H, Xiao AM, Wang C, Huang X, Yan PS, Liu ZH, Qu XF, Belinson JEROMEL, Wu RF. Song FB, et al. Zhonghua Fu Chan Ke Za Zhi. 2020 Nov 25;55(11):784-790. doi: 10.3760/cma.j.cn112141-20200520-00428. Zhonghua Fu Chan Ke Za Zhi. 2020. PMID: 33228350 Chinese.

• P16(INK4a) immunocytochemistry in liquid-based cytology samples in equivocal Pap smears: added value in management of women with equivocal Pap smear.

  Monsonego J, Pollini G, Evrard MJ, Sednaoui P, Monfort L, Quinzat D, Dachez R, Syrjänen K. Monsonego J, et al. Acta Cytol. 2007 Sep-Oct;51(5):755-66. doi: 10.1159/000325839. Acta Cytol. 2007. PMID: 17910346

• p16INK4a immunocytochemistry versus human papillomavirus testing for triage of women with minor cytologic abnormalities: a systematic review and meta-analysis.

  Roelens J, Reuschenbach M, von Knebel Doeberitz M, Wentzensen N, Bergeron C, Arbyn M. Roelens J, et al. Cancer Cytopathol. 2012 Oct 25;120(5):294-307. doi: 10.1002/cncy.21205. Epub 2012 Jun 14. Cancer Cytopathol. 2012. PMID: 22700382 Free PMC article. Review.

• p16(INK4a) immunostaining in cytological and histological specimens from the uterine cervix: a systematic review and meta-analysis.

  Tsoumpou I, Arbyn M, Kyrgiou M, Wentzensen N, Koliopoulos G, Martin-Hirsch P, Malamou-Mitsi V, Paraskevaidis E. Tsoumpou I, et al. Cancer Treat Rev. 2009 May;35(3):210-20. doi: 10.1016/j.ctrv.2008.10.005. Epub 2009 Mar 3. Cancer Treat Rev. 2009. PMID: 19261387 Free PMC article. Review.

• Prevention of Cervical Cancer: Guideline of the DGGG and the DKG (S3 Level, AWMF Register Number 015/027OL, December 2017) - Part 2 on Triage, Treatment and Follow-up.

  Hillemanns P, Friese K, Dannecker C, Klug S, Seifert U, Iftner T, Hädicke J, Löning T, Horn L, Schmidt D, Ikenberg H, Steiner M, Freitag U, Siebert U, Sroczynski G, Sauerbrei W, Beckmann MW, Gebhardt M, Friedrich M, Münstedt K, Schneider A, Kaufmann A, Petry KU, Schäfer APA, Pawlita M, Weis J, Mehnert A, Fehr M, Grimm C, Reich O, Arbyn M, Kleijnen J, Wesselmann S, Nothacker M, Follmann M, Langer T, Jentschke M. Hillemanns P, et al. Geburtshilfe Frauenheilkd. 2019 Feb;79(2):160-176. doi: 10.1055/a-0828-7722. Epub 2019 Feb 18. Geburtshilfe Frauenheilkd. 2019. PMID: 30792546 Free PMC article.

• Detecting cervical precancer and reaching underscreened women by using HPV testing on self samples: updated meta-analyses.

  Arbyn M, Smith SB, Temin S, Sultana F, Castle P; Collaboration on Self-Sampling and HPV Testing. Arbyn M, et al. BMJ. 2018 Dec 5;363:k4823. doi: 10.1136/bmj.k4823. BMJ. 2018. PMID: 30518635 Free PMC article.

• Comparison of diagnostic accuracy and acceptability of self-sampling devices for human Papillomavirus detection: A systematic review.

  Aimagambetova G, Atageldiyeva K, Marat A, Suleimenova A, Issa T, Raman S, Huang T, Ashimkhanova A, Aron S, Dongo A, Iztleuov Y, Shamkeeva S, Azizan A. Aimagambetova G, et al. Prev Med Rep. 2024 Jan 4;38:102590. doi: 10.1016/j.pmedr.2024.102590. eCollection 2024 Feb. Prev Med Rep. 2024. PMID: 38283967 Free PMC article. Review.

• Involvement of PSMD10, CDK4, and Tumor Suppressors in Development of Intrahepatic Cholangiocarcinoma of Syrian Golden Hamsters Induced by Clonorchis sinensis and N-Nitrosodimethylamine.

  Uddin MH, Choi MH, Kim WH, Jang JJ, Hong ST. Uddin MH, et al. PLoS Negl Trop Dis. 2015 Aug 27;9(8):e0004008. doi: 10.1371/journal.pntd.0004008. eCollection 2015. PLoS Negl Trop Dis. 2015. PMID: 26313366 Free PMC article.

RetroSearch is an open source project built by @garambo | Open a GitHub Issue

Search and Browse the WWW like it's 1997 | Search results from DuckDuckGo

HTML: 3.2 | Encoding: UTF-8 | Version: 0.7.3