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Showing content from https://pubmed.ncbi.nlm.nih.gov/16078195/ below:

Cell nanosurgery using ultrashort (femtosecond) laser pulses: applications to membrane surgery and cell isolation

Affiliations

Affiliation

¹ Department of Electrical and Computer Engineering, Ultrafast Photonics and Nano-Optics Laboratory, University of Alberta, Edmonton, Alberta T6G 2V4, Canada.

PMID: 16078195
DOI: 10.1002/lsm.20220

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Cell nanosurgery using ultrashort (femtosecond) laser pulses: applications to membrane surgery and cell isolation

Vikram Kohli et al. Lasers Surg Med. 2005 Sep.

Affiliation

¹ Department of Electrical and Computer Engineering, Ultrafast Photonics and Nano-Optics Laboratory, University of Alberta, Edmonton, Alberta T6G 2V4, Canada.

PMID: 16078195
DOI: 10.1002/lsm.20220

Item in Clipboard

Abstract

Membrane surgery and nanosurgical cell isolation using high-intensity femtosecond laser pulses is reported. We demonstrate the applicability of using ultrashort (femtosecond) laser pulses for performing surgery on live mammalian cells. When sub-10 femtosecond pulses were focused onto the cell, precise sub-micron surgical cuts were made on the biological membrane. Traversing the cells relative to the focused laser spot, we report on localized nanosurgical ablation of focal adhesion adjoining epithelial cells. In each study, the surgery was conducted without morphologically compromising the cells.

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