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WO2020128033A1 - Cellular metabolism of hiv-1 reservoir seeding in cd4+ t cells

WO2020128033A1 - Cellular metabolism of hiv-1 reservoir seeding in cd4+ t cells - Google PatentsCellular metabolism of hiv-1 reservoir seeding in cd4+ t cells Download PDF Info
Publication number
WO2020128033A1
WO2020128033A1 PCT/EP2019/086782 EP2019086782W WO2020128033A1 WO 2020128033 A1 WO2020128033 A1 WO 2020128033A1 EP 2019086782 W EP2019086782 W EP 2019086782W WO 2020128033 A1 WO2020128033 A1 WO 2020128033A1
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WO
WIPO (PCT)
Prior art keywords
cells
hiv
metabolic
infection
infected
Prior art date
2018-12-20
Application number
PCT/EP2019/086782
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French (fr)
Inventor
Asier Saez-Cirion
Jose Carlos VALLE-CASUSO
Michaela Muller-Trutwin
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Institut Pasteur
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2018-12-20
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2019-12-20
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2020-06-25
2019-12-20 Application filed by Institut Pasteur filed Critical Institut Pasteur
2020-06-25 Publication of WO2020128033A1 publication Critical patent/WO2020128033A1/en
Links Classifications Definitions Landscapes Abstract

HIV persists in long-lived infected cells that are not affected by antiretroviral treatment. These HIV reservoirs are mainly located in CD4+ T-cells, but their distribution is variable in the different subsets. Susceptibility to HIV-1 increases with CD4+ T-cell differentiation. We evaluated whether the metabolic programming that supports the differentiation and function of CD4+ T-cells affected their susceptibility to HIV-1. We found that differences in HIV- susceptibility between naïve and more differentiated subsets were associated with the metabolic activity of the cells. Indeed, HIV-1 selectively infected CD4+ T-cells with high oxidative phosphorylation and glycolysis, independent of their activation phenotype. Moreover, partial inhibition of glycolysis (i) impaired HIV-1 infection in vitro in all CD4+ T-cell subsets, (ii) 10 decreased the viability of pre-infected cells, and (iii) precluded HIV-1 reactivation in cells from HIV-infected individuals. Our results elucidate the link between cell metabolism and HIV- infection and identify a vulnerability to tackle HIV reservoirs and infections with other pathogens.

Description

CELLULAR METABOLISM OF HIV-1 RESERVOIR SEEDING IN CD4+ T CELLS

FIELD OF THE INVENTION

The present invention relates to compounds and methods for partially inhibiting of cellular metabolism, and particularly glycolysis, in cells targeted by pathogens, such as human immunodeficiency viruses.

BACKGROUND TO THE INVENTION

Combination antiretroviral treatment (cART) blocks HIV-1 replication but does not eliminate infected cells. Replication competent HIV-1 persists in cellular reservoirs that are the origin of rapid viral rebound when treatment is interrupted (Finzi et al., 1997). Identifying the factors underlying the seeding and survival of HIV-infected cells is a priority in the search for an HIV cure (Deeks et al., 2016). CD4+ T-cells are the major target for HIV-1 infection and are thought to constitute most of the HIV-1 reservoir. However, not all CD4+ T-cells contribute equally to the pool of persistently infected cells during cART. The composition of CD4+ T-cells that remain infected is mainly determined by the susceptibility of CD4+ T-cell subsets to HIV infection, their resistance to HIV-induced apoptosis and their life span and turnover potential (Barton et al., 2016). Naive CD4+ T-cells are highly resistant to HIV-1 infection, while HIV-1 susceptibility increases in more differentiated cell subsets (Roederer et al., 1997; Schnittman et al., 1990). Accordingly, there is a minimal contribution of naive CD4+ T-cells to the HIV reservoir during cART, which is mainly restricted to the memory cell subsets (Chomont et al., 2009). The susceptibility of CD4+ T-cells to HIV-1 infection depends on the relative abundance of cell factors required by the virus to complete its replication cycle and of cellular restriction factors that counteract infection (Lever and Jeang, 2011). T-cell activation sharply increases the expression of HIV dependency factors and thereby cell susceptibility to HIV-1 infection (Pan et al., 2013; Stevenson et al., 1990), despite the concomitant presence of some restriction factors that the virus can most often circumvent. However, responsiveness to TCR activation (Byrne et al., 1988; Roederer et al., 1997) and susceptibility to HIV infection are not homogeneous across or within CD4+ T-cell subsets. This discrepancy in infection efficacy suggests that HIV-1 has adapted to infect CD4+ T-cells with a specific cellular program (Cleret-Buhot et al., 2015). The cellular processes orchestrating the optimal conditions for the establishment of HIV-1 infection remain unclear.

Numerous studies have demonstrated the role of cellular metabolism in T-cell immunity (Pearce et al., 2013; Waickman and Powell, 2012). Naive T-cells circulate in a quiescent state, relying essentially on oxidative phosphorylation (OXPHOS). Upon T-cell activation and after receiving appropriate cues (costimulation, cytokines), naive T-cells undergo metabolic reprogramming, strongly increasing OXPHOS and, especially, glycolysis, to cope with the energy demands of immune function and rapid proliferation (Pearce et al., 2013). The biomass accumulation that accompanies enhanced cellular metabolism may provide viruses with the abundance of factors that are necessary for their replication. It is worth noting that several retroviruses have evolved to use metabolite transporters as cellular receptors. The glucose transporter 1 (GLUT) is the main receptor for HTLV-1 (Manel et al., 2003); phosphate transporters PiTl and PiT2 have been reported as surface receptors for koala retrovirus, feline leukemia virus and murine leukemia viruses (Oliveira et al., 2006; Takeuchi et al., 1992; von Laer et al., 1998); and the amino acid transporters ASCT1 and ASCT2 are the receptors for the feline RD-114 endogenous retrovirus (Shimode et al., 2013). Although HIV-1 does not use metabolite transporters as its main receptors, GLUT1 expression is necessary for the post entry steps of HIV-1 replication in CD4+ T-cells (Loisel-Meyer et al., 2012). Moreover, the metabolism of nucleotides is critical for HIV-1 reverse transcription (Amie et al., 2013).

BRIEF SUMMARY OF THE INVENTION

The invention encompasses compositions comprising metabolic inhibitors and methods of using these compositions.

In one embodiment, the invention encompasses a method of reducing HIV production from a T cell comprising contacting a population of cells comprising HIV-infected CD4+ T cells with 2-deoxy-glucose (2-DG) at a concentration that reduces the production of HIV from said HIV-infected CD4+ T cells. In one embodiment, the cells are exposed to a concentration of 1-25 mM 2-DG. In one embodiment, the cells are exposed to a concentration of 3-10 mM 2-DG. In one embodiment, the cells are exposed to a concentration of 5 mM 2-DG. In one embodiment, the method further comprises contacting the population of cells with a metabolic inhibitor that blocks fatty acid transport to mitochondria. In one embodiment, the metabolic inhibitor is Etomoxir.

In one embodiment, the cells are contacted with the metabolic inhibitor in vitro. In one embodiment, the cells are contacted with the metabolic inhibitor in vivo. In one embodiment, the cells are contacted with an additional metabolic inhibitor of metabolic function in CD4+ T cells.

In one embodiment, the population of cells has received cART treatment. The cART can comprise Combivir, Kaletra, Trizivir, Epzicom, Kivexa, Truvada, Atripla, Complera, Eviplera, Stribild, Triumeq, Evotaz, Prezcobix, Dutrebis, Genvoya, or Descovy. The cART can comprise at least 2 or 3 of any of the following compounds: lamivudine; zidovudine; lopinavir; ritonavir; abacavir; tenofovir disoproxil fumarate; emtricitabine; efavirenz; rilpivirine; elvitegravir; cobicistat; dolutegravir; atazanavir; cobicistat; darunavir; and raltegravir.

In one embodiment, the invention encompasses a method of reducing virus or bacterial pathogen production from a cell comprising contacting a population of cells pathogen-infected cells with at least 2 metabolic inhibitors of different metabolic functions in the cells at a concentration that reduces the production of the pathogen from said pathogen-infected cells.

In one embodiment, one of the metabolic inhibitors blocks glycolysis. In one embodiment, one of the metabolic inhibitors blocks fatty acid transport to mitochondria. In one embodiment, both of the metabolic inhibitors are selected from Table 1. In one embodiment, two of the metabolic inhibitors are DON and 2DG. In one embodiment, three of the metabolic inhibitors are DON and etomoxir and 2DG. In one embodiment, concentration of the metabolic inhibitor(s) is a suboptimal concentration.

In one embodiment, the pathogen is HIV-1.

In one embodiment, the invention encompasses a method for measuring the effect of a metabolic inhibitor of metabolic function in cells in a pathogen-infected human comprising: administering at least one dose of a metabolic inhibitor of metabolic function in cells to the human; and measuring the level of pathogen infection in the pathogen-infected human. In one embodiment, measuring the level of pathogen infection in the pathogen-infected human comprises measuring the level of plasma HIV RNA in an HIV-infected human. In one embodiment, measuring the level of plasma HIV RNA in the HIV-infected human is performed by a reverse transcription and amplification reaction. In one embodiment, the level of HIV infection in the human is measured at least 2, 3, 4, 5, 6, 7, 8, 9, or 10 times.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1A-D depict CD4+ T-cells subsets have different susceptibilities to HIV-1 infection. Figure 1A depicts representative example of the proportion of 5-days activated CD4+ T cells expressing GFP in the absence of infection (top) or 72 h after challenge with HIV-1GFP-VSV (bottom). Figure IB depicts relative distribution of CD4+ T-cell subsets in non-activated (NA) and activated (aCD3 5d) cells before HIV challenge and in activated cells not expressing GFP (aCD3 5d GFP-) or expressing GFP (aCD3 5d GFP+) 72 h post challenge. The pie charts (top) represent the median values (n=3 donors). The bottom panels represent the fold change in the CD4+ T- cells subset contribution relative to the non-activated condition (NA). *p<0.05; ** p<0.01. In a different set of experiments, sorted CD4+ T-cell subsets were cultured under NA or activated conditions for 3 (3d) or 5 days (5d) and challenged with HIV-1GFP-VSV. Figure 1C depicts representative example of infection levels in Tn, Tcm, Ttm and Tern cells from a donor in the different conditions analyzed. Figure ID depicts Medians and IQR values for experiments with cells from 6 donors. Symbols represent the individual data points. Significant differences between experimental conditions are shown for each T-cell subset as horizontal lines. The median infection level in NA Tn cells is displayed as a reference dashed line to facilitate comparison between T-cell subsets.

Figure 2A-B depict HIV-1 infection levels in CD4+ T-cell subsets correlate with the expression levels of genes related to cell metabolism. Figure 2A shows Heat maps displaying the genes differentially expressed (p<0.05) between the CD4+ T-cell subsets (Tn, Tcm, Ttm and Tern) (n= 6 donors) in the absence of activation or after 3 or 5 days of activation with soluble anti-CD3 (i.e., at the time of HIV challenge) (green = downregulation, red = upregulation). Variables are ordered by hierarchical clustering and samples by CD4+ T-cell subsets. Figure 2B shows Spearman's correlation between the levels of gene expression at the time of HIV-1 challenge and HIV-1 infection levels 72 h after challenge. Only significant correlations (p<0.05) are represented in the graphs (green bars). Genes highlighted in red show the group of genes that correlated with infection levels in all conditions.

Figure 3A-C depict CD4+ T-cell subsets have different metabolic profiles that coincide with their susceptibility to HIV-1 infection OCR and ECAR in non-activated (NA), 3-day activation (3d) and 5-day activation (5d) CD4+ T-cell subsets. Figure 3A depicts Median values of the metabolic variables obtained for the CD4+ T-cell subsets from the 6 donors in the different conditions analyzed. Figure 3B depicts Median and IQR basal OCR (left panel) and ECAR (right panel). Figure 3B depicts basal ECAR/OCR ratio for CD4+ T-cell subsets in different activation states (Od, 3d, 5d). Median values in NA Tn cells are indicated by dashed lines as a reference. Symbols represent independent experiments (n=6). Figure 3C shows a Summary of correlations between metabolic parameters at the time of infection in NA, 3d and 5d activated CD4+ T-cell subsets and the % of infected cells 72 h post infection. The green color indicates p<0.05. The size of the circle represents Spearman's coefficients.

Figure 4A-C depict HIV-l-infected CD4+ T-cells are characterized by higher metabolic activity levels. Figure 4A depicts Metabolic activity (OCR and ECAR) of sorted HIV-infected GFP+ and non-infected GFP- CD4+ T-cells (n=3). The bioenergetic (XF) phenotypes of GFP+ and GFP- cells (right panel) were determined by the basal OCR and ECAR values. The symbols represent independent experiments (n=3 donors). Figure 4B depicts In a different set of experiments, CD4+ T-cells were sorted 72 h after HIV challenge based first on their activation levels (high activation, CD25+/HLA-DR+ or low activation, CD25-/HLA-DR-) and then on the level of GFP expression (GFP- or GFP+ cells). The gating strategy is shown on the left panels. Pie charts (right) represent the median (n=4 donors) distribution of the CD4+ T-cell subsets (determined by flow cytometry) for each sorted cell fraction as follows: high activation and GFP+, high activation and GFP-, low activation and GFP+ and low activation and GFP- (n=4). Figure 4C depicts Representative analyses of OCR and ECAR (measured as above) for each cell fraction (left) and the median and IQR basal OCR and ECAR for 6 (high activation) and 4 (low activation) donors (right). Figure 5A-B depicts rate of glucose uptake by CD4+ T-cell subsets is associated with their susceptibility to HIV-1 infection CD4+ T cells were sorted based on their differentiation status (Tn or Tcm) and their rate of 2NBDG uptake. Sorted cells were then challenged with HIV-lGFp- VSV. Figure 5A depicts Representative example of 2NBDG content after sorting (top panels) and the levels of GFP expression 72 h after challenge in CD4+ T-cell fractions exposed (HIV-1) or not (control) to the virus. Figure 5B depicts Percentage of GFP-positive cells among CD4+ T-cell fractions (Tn and Tcm sorted depending on their pre-infection 2-NBDG uptake). Symbols represent individual values (n=3, Tn; n=5, Tcm) donors. Medians and IQR values are represented by horizontal lines.

Figure 6A-F depict inhibition of cell metabolic pathways blocks HIV-1 infection of CD4+ T- cells. Figure 6A depicts Relative level of infection (blue bars) and cell death (purple bars) compared to the control conditions in 5-days activated CD4+ T-cells infected in the absence or presence of increasing amounts of Etomoxir, DON, or 2-DG (median and IQR, n=3 donors). Figure 6B depicts Infection and cell death in CD4+ T-cells exposed to HIV-1 in glucose-containing medium in the absence or presence of 2-DG (5 mM) or in culture medium without glucose (starvation) (left) or in the absence or presence of UK5099 (25 mM) (right). Figure 6C depicts Relative number of U5-Gag copies in CD4+ T-cells at 6h, 15h or 72h after infection with H IV-1GFP- VSV in the absence or presence of 2-DG. Individual values (symbols), medians and IQRs (horizontal lines) for five different donors are shown. Figure 6D depicts Infection levels and number of U5-Gag copies 72h after challenge with HIV-lGFp-VSV in the absence or presence of 2- DG added at the time of challenge, 4h or 8h post challenge. Values represent the relative levels of infection compared to the control condition (median and IQR, n=3 donors). Figure 6E depicts Changes in HIV-1 infection levels in CD4+ T-cell subsets 72h after the infection of bulk CD4+ T- cells in the absence or in presence of 2-DG (orange symbols) or Etomoxir (beige symbols). Medians (n=7 donors) are shown. Figure 6F depicts Percentage of HIV-1 productively (left panel) or latently (right panel) infected cells 72h after the infection of CD4+ T-cells with HIV-lDuoFluo VSVG particles in the presence of 2-DG or etomoxir. Median and IQR values from experiments with 6 donors are shown. Figure 6G depicts p24 production in supernatants from CD4+T-cell cultures 3 and 7 days after infection with HIV-1 BaL in the absence (blue bars) or presence of 2- DG (5 mM) (orange bars). Means and standard deviations for three replicates are shown at each time point for experiments done with cells from three different donors.

Figure 7A-D depict suboptimal inhibition of glucose metabolism selectively eliminates preinfected CD4+ T-cells and inhibits HIV-1 amplification from reservoirs. Figure 7A depicts cell viability in sorted pre-infected GFP+ (green) or noninfected GFP- (red) CD4+ T cells cultured for 48 h in the absence or presence of 2-DG. One representative example is shown. Figure 7B depicts relative survival of 2-DG treated cells (circles) was compared to that of nontreated cells (squares) at 24 h and 48 h. Figure 7C depicts changes in the CD4+ T-cell subset distribution 48h after the treatment of infected bulk CD4+ T-cells with 2-DG when compared with the distribution in the control condition. Median values and IQR are shown (n=3 donors). Figure 7D depicts HIV-1 reactivation from CD4+ T-cells from six individuals on cART upon PHA/IL-2 stimulation in the absence (blue line/symbols) or presence of 2-DG (5 mM) (orange line/symbols) (mean and SD, 3 replicates). Mean p24 values in the absence or presence of 2-DG on day 14 post stimulation are shown for all six experiments (right panel).

Figure 8 depicts treatment of CD4+ T cells from the same donor with various metabolic inhibitors at the indicated concentrations, alone or in combination, and shows the synergistic effect of suboptimal amounts of 2DG, DON and ETOMOXIR on the extent of HIV-1 infection.

Figure 9, related to STAR methods

A representative example of the flow cytometry gating strategy used to sort CD4+ T cell subsets. Naive (Tn; CD4-CD45RA+, CCR7+, CD27+, CD95-), central memory (Tcm; CD4-CD45RA-, CCR7+, CD27+), transitional memory (Ttm; CD4-CD45RA-, CCR7-, CD27+) or effector memory (Tern; CD4-CD45RA-, CCR7-, CD27-) displayed as standard pseudocolor dot plots. These sorted CD4+ T cell subsets were used in the gene expression analyses, metabolic profile assays and HIV-1 susceptibility assays. Table with gender, age and relative CD4+ T cell subset distribution ex vivo for each of the donors studied in the gene expression analyses, metabolic profile assays and HIV-1 susceptibility assays.

Figure 10, related to Figure 2

Spearman's correlation between the levels of expression of all genes analyzed at the time of HIV-1 challenge and HIV-1 infection levels 72 h after challenge for nonactivated (NA), 3- day activation (3d) or 5-day activation (5d) CD4+ T cells. Significant correlations (p<0.05) are shown in green. Venn diagram and table showing the genes correlating with infection in the different activation conditions.

Figure 11, related to Figure 3

A) Representative example of flow cytometry results at the end of the glucose uptake assay showing levels of 2-NBDG in nonactivated and 5-day activation CD4+ T cells. We defined the following 3 gates based on 2-NBDG capture levels: low, medium and high (upper panels). The levels of 2-NDBG captured by the different CD4+ T cell subsets are shown in the overlapping histogram (middle panels). The relative contributions of CD4+ T cell subsets to the low, medium and high 2-NBDG cell fractions are displayed in the cumulative bar chart (lower panels). B) A representative example of GLUT1 surface expression (left panel) and glucose uptake (right panel) (2-NBDG assay) in the CD4+ T cell subsets studied here after 5 days of suboptimal activation. C) Correlation between metabolic parameters (basal OCR, maximal respiratory capacity, basal and maximal ECAR measure with a Seahorse analyzer) at the time of infection in NA, 3d and 5d activated CD4+ T cell subsets and the % of infected cells 72 h post infection. Each symbol represents values for values for one CD4+ T cell subset from one individual. The p values of Spearman correlation analyses are shown. Lines depict linear regression of analyzed data.

Figure 12, related to Figure 4

A) Representative example of the flow cytometry gating strategy used to sort noninfected GFP- and infected GFP+ CD4+ T cells 72 h after challenge with single-cycle H IV-1 GFP particles and G FP expression in the purified populations. Representations are displayed as standard pseudocolor dot plots (left). Representative example of the glycolytic activity of GFP+ and GFP- sorted cells analyzed before and after the addition of glucose, oligomycin and 2-DG (right). B) Percentage of cells among each CD4+ T cell subset expressing HLA-DR and CD25 five days after suboptimal activation with aCD3 (upper panel). Black horizontal lines above the symbols denote statistically significant differences subsets. C) Representative example of expression levels of CD25 and HLA-DR for CD4+ T cell fractions sorted as high activation and GFP+, high activation and GFP-, low activation and GFP+ and low activation and GFP-. D) Changes in the relative contribution of CD4+ T cell subsets to the GFP+ fractions when compared to their GFP- counterparts. Asterisks represent statistically significant differences (*p<0.05; ** p<0.01).

Figure 13, related to STAR methods and Figure 5

A) Flow cytometry gating strategy used to sort CD4+ Tn and Tcm cells according to their glucose (2- N BDG ) u pta ke levels a nd the 2-N BDG content of the CD4+ T cel l fractions after sorti ng. Representations are displayed as standard pseudocolor dot plots. Sorted cells were used for HIV-1 susceptibility assays. B) Representative example (left) and summary (median and IQR, n=3 donors) (right) of the glycolytic activity of sorted Tcm cells with high glucose uptake (TcmHG Iu) and low glucose uptake (TcmLGIu).

Figure 14, related to Figure 6

Representative example of the metabolic profile of five-day activation CD4+ T cells after bei ng treated with increased concentrations of the metabolic inhibitors Etomoxir (top panel) and 2-DG (middle panel). OCR/ECAR basal ratio after the administration of 2-DG and Etomoxir (Lower panel).

Figure 15, related to Figure 6 A) A representative example of GFP and mCherry expression in noninfected CD4+ T cells or in CD4+ T cells 72 h post infection with the HIV Duo- Fluo I virus. As described in (Calvanese et al., 2013), cells that expressed GFP alone or in combination with mCherry were considered productively infected (I), cel ls that expressed only mCherry were considered latently infected (L), and cells that lacked expression of both fluorescent markers were considered noninfected (Nl). Pie chart diagrams show the median (n=5 donors) contribution of CD4+ T cell subsets to the pool of H IV-1 prod uctively infected (upper panel) or latently infected cells (lower panel) 72 h after infection of nonactivated (NA), 3-day activation (3d) or 5-day activation (5d) CD4+ T cells challenged with the HIV-1 DuoFluo I virus. B) Relative infection levels (with respect to naive cells) in 5 days-activated CD4+ T cells from donors (n=9) 72h after chal lenge with H IV Bal or VSVG pseudotyped N L4.3AenvGFP particles. I nfection levels were determined by flow cytometry quantification of intracellular p24 and G FP respectively (top panels). Changes in the distribution of cell subsets in HIV+ cells in relation to non- infected cells (bottom panels). The asterisks represent statistically significant differences (*p<0.05; ** p<0.01; *** p<0.001). C) I nfection levels in 5 days

activated CD4+ T cells 72 h post challenge with HIV-1 Bal in the absence or presence of 2-DG added at the time of challenge, 4h or 8h post challenge. Bl ue bars represent the relative level of i nfection (determined by intracel l ular p24 staining) compared to the control conditions (median and IQR, n=3 donors). Horizontal lines indicate statistical differences.

DETAILED DESCRIPTION OF THE INVENTION

In the present study, we undertook the analysis of the conditions determining the intracellular susceptibility of CD4+ T-cell subsets to HIV-1 infection. In particular, we analyzed whether the metabolic program is distinct according to the differentiation of CD4+ T-cell subsets and if this determines their susceptibility to HIV-1 infection. We show that cellular metabolism is a central factor driving the HIV-1 infection of CD4+ T-cells and that it may be an important target for new therapies against HIV-1.

In this study, we performed a detailed characterization of the bioenergetics of CD4+ Tn, Tcm, Ttm and Tern cells. Upon potent TCR activation, naive and memory cells have been shown to strongly upregulate their metabolism and acquire effector functions (van der Windt et al., 2013). Here, we show important metabolic differences among the three memory cell populations studied, even in the absence of stimulation. Upon anti-CD3 activation, all CD4+ T- cell subsets enhanced their metabolic activity but essentially maintained their distinctive metabolic programs, which matched the requirements for their expected rapid reaction to antigenic stimulation (Tem»Ttm>Tcm»Tn). The metabolic activity of the T-cell subsets overlapped with their susceptibility to HIV-1 infection (Figures 1C and 3B), supporting that the extent of HIV-1 infection in CD4+ T-cell subsets was affected by the metabolic environment within the target cells.

Transcript profiling at the time of infection showed that among the CD4+ T-cell subsets, there were positive correlations between the frequencies of HIV-infected cells and the expression levels of multiple genes related to cell metabolism. Negative correlations were found between the susceptibility of CD4+ T-cells to HIV-1 infection and the expression of SAMHD1, an efficient HIV-1 restriction factor that also plays an important role in the regulation of cell metabolism (Descours et al., 2012; Mathews, 2015). Surprisingly, strong positive correlations were found between the levels of HIV-infected cells and the expression of a cluster of genes related to the interferon response. Although this point was not specifically explored in the present study, increasing evidence has revealed the interrelationships between cell metabolism and the interferon response (Burke et al., 2014; Zhao et al., 2015). Some type 1 interferons might enhance glycolysis (Fritsch and Weichhart, 2016), and interferon regulatory factors play a key role during the metabolic reprogramming that follows TCR-mediated activation of T-cells (Man et al., 2013). The interaction between the interferon response and cell metabolism may somewhat explain the dichotomy between antiviral and viral-enhancing interferon-stimulated genes (Schoggins and Rice, 2011; Seo et al., 2011). Tern cells, which were the most susceptible to HIV infection in our assay, expressed the strongest levels of several restriction factors such as SLFN11 or APOBEC3G. Our results thus indicate that HIV-1 exploits the metabolic environment that most favors the completion of its replication cycle, and this might be one of the factors underlying the adaptation of HIV-1 to evade some restriction factors.

We further confirmed the association between T-cell metabolism and HIV infection in a series of functional analyses. First, we showed that HIV-infected CD4+ T-cells had higher levels of metabolic activity and metabolic potential than HIV-exposed but noninfected cells. This was not solely the consequence of the preferential infection of cells with higher activation levels; when we sorted CD4+ T-cells that were matched for the expression of common activation markers, we still found that HIV-infected cells had higher metabolic activity levels than noninfected CD4+ T-cells. Although there are well-established links between T-cell activation and cellular metabolism, it is increasingly clear that T-cell functions, including proliferation, the secretion of cytokines and cell survival, are supported through different engagements of the various metabolic pathways (Jones and Bianchi, 2015). This may explain the partial dichotomy between T-cell activation and cell metabolism in HIV infection that we observed in our experiments. Additionally, we found Tn cells expressing high levels of activation markers upon anti-CD3 stimulation, but these cells remained mostly resistant to HIV-1 infection. In contrast, the frequency of infected Tn cells sharply increased when we challenged highly glycolytic Tn cells. This is in agreement with previous results that showed that expression of GLUT1 is necessary for HIV-1 infection of CD4+ T-cells (Loisel-Meyer et al., 2012) and that, in vitro, HIV preferentially infects CD4+ T-cells expressing GLUT1 and 0X40 (Palmer et al.). Overall, our results demonstrate that cells that had higher metabolic activity levels were more susceptible to HIV infection.

In our experimental conditions, we could detect virtually no infected cells when we challenged cells with low metabolic activity levels. Thus, any potential change in cell metabolism that might have been induced directly by HIV particles was not sufficient to promote infection in cells that had low metabolic activity levels at the time of viral challenge. However, it is important to note that because we were interested in understanding the factors modulating HIV infection beyond the expression of HIV receptors, we used single-cycle particles devoid of HIV envelope and pseudotyped with VSV-G in this set of experiments. It is possible that fully replication-competent viruses have a stronger effect on modulating CD4+ T-cell metabolism. CCL5 engagement with CCR5 has been described as increasing glycolysis in T-cells (Chan et al., 2012), and it is possible that gpl20 triggers a similar effect. Moreover, HIV infection has been shown to induce increased expression of several glucose transporters in in vitro experiments (Kavanagh Williamson et al., 2018; Sorbara et al., 1996). Overall, viruses appear to possess different mechanisms to enhance cell metabolism to favor viral replication (Goodwin et al., 2015; Sanchez and Lagunoff, 2015), and this deserves additional exploration in the context of HIV infection.

Suboptimal inhibition of glycolysis impaired HIV replication, and this was observed with single-cycle VSVG pseudotyped particles and replication-competent HIV-1 Bal and for all CD4+ T- cell subsets, although the effects were more pronounced in more energetic cells. Inhibition of glycolysis, including several hours after viral entry, severely reduced the accumulation of HIV reverse transcripts and impaired the establishment of both productive and latent infections. Our results thus point to critical steps early during the viral replication cycle (in particular reverse transcription) that are influenced by glycolysis, which agrees with a previous report (Loisel-Meyer et al., 2012). Along these lines, the synthesis of deoxynucleotides, the level of which is a limiting factor for HIV reverse transcription, is very energy demanding and requires substrates that are provided by different metabolic pathways, such as the pentose phosphate pathway (PPP) that is parallel to glycolysis (Lane and Fan, 2015). Although, unfortunately, genes involved in the PPP were not included in our gene expression panel, we found important differences between CD4+ T-cell subsets and strong correlations with infection levels for several genes such as TP53, ESF1 and RRM2, which play critical roles in the de novo synthesis of dNTPs. In particular we have recently shown that changes in the expression of RRM2 impact HIV-1 replication in macrophages and dendritic cells by modifying the pools of dNTPS (Allouch et al., 2013; Valle-Casuso et al., 2017). Moreover, SAMHD1, the expression levels of which were negatively correlated with infection in our analysis, is a deoxynucleoside triphosphohydrolase that contributes to control the intracellular dNTP concentration during cell-cycle (Mathews, 2015). Our results therefore suggest that metabolically active cells offer an environment with positive synthesis (RRM2) vs degradation (SAMHD1) of dNTP pools that favors HIV-1 reverse transcription. However, other steps of the viral replication cycle may also depend on cell metabolism. The inhibition of glycolysis has been shown to decrease the production of HIV-1 particles (Hegedus et al., 2014), and mTOR, a key regulator of cellular metabolism (Waickman and Powell, 2012), appears to be involved in the establishment of HIV-1 latency in CD4+ T-cells (Besnard et al., 2016).

In our functional experiments we mostly focused on assessing the impact of glycolysis on HIV infection. Our results showing that inhibition of pyruvate transport to the mitochondria with UK5099 blocked HIV infection suggests that glucose oxidation is important for HIV-1 infection. However the relative contribution of aerobic vs oxidative glycolysis remains to be determined. It is likely that other metabolic functions are also important for HIV-1 infection. The inhibition of fatty acid oxidation with Etomoxir had a limited effect on HIV replication in suboptimal conditions, mostly in Tern cells, but it strongly inhibited infection at higher concentrations. However, caution is needed when interpreting results obtained with Etomoxir as it has been shown to produce off target effects at such high concentrations (O'Connor et al., 2018; Yao et al., 2018). A recent report suggested that fatty acid metabolism may also participate in the late steps of viral replication (Kulkarni et al., 2017). Our results with the glutamine antagonist DON suggest that glutamine metabolism may also be necessary for the optimal infection of CD4+ T-cells. In general, the association between HIV infection and cell metabolism can be exploited to impair HIV-1 replication.

Cell survival is another process regulated by cell metabolism that could be critically relevant for the persistence of infected cells. We found that suboptimal inhibition of glycolysis induced the selective death of cells that had been preinfected in vitro, and this affected all CD4+ T-cell memory subsets. We also show here that the partial inhibition of glycolysis in CD4+ T-cells from HIV-infected individuals on cART potently blocked viral reactivation and spread. Based on our results, this could be the result of a combination of both the elimination of infected cells and the blocking of new cycles of viral amplification by 2-DG. Overall our results point to the potential modulation of cell metabolism as a strategy to combat HIV infection.

Therapies targeting cellular metabolism are gaining interest in the cancer field (Zhao et al., 2013). Metabolic reprogramming observed in tumor cells closely resembles the metabolic profile of HIV-infected T-cells that we describe here. In the context of the physiopathology of HIV infection, high glucose consumption by infected CD4+ T-cells could have additional implications for immune responses. We recently found that while HIV-specific CD8+ T-cells from rare individuals naturally controlling HIV infection are characterized by metabolic plasticity, HIV- specific CD8+ T-cells from most HIV-infected subjects heavily rely on glycolysis to exert their functions (Angin et al, submitted). High levels of glucose consumption by CD4+ T-cells at the sites of viral replication might severely limit glucose availability for these CD8+ T-cells and impair their effector function. In addition, lactic acid, which is a product of glycolysis, inhibits effector functions in cytotoxic T-cells (Mendler et al., 2012). Therefore, the metabolic characteristics of HIV-infected CD4+ T-cells may provide the virus with additional mechanisms to mediate immune evasion, as has also been described for tumors (Sugiura and Rathmell, 2018). Because exploiting the host cell metabolic machinery appears to be a common strategy for invading pathogens, including viruses, bacteria and parasites, therapies targeting cell metabolism could affect a large spectrum of infections. Obviously, cell metabolism regulates critical physiological events, including immune responses, and it is necessary to develop a better understanding of the links between cell metabolism and acute and chronic infections. Overall, our study shows that cellular metabolism is a central factor that drives the HIV-1 infection of CD4+ T-cells more strongly than does the state of differentiation and/or activation, and cellular metabolism may be an important target for new therapies against HIV-1.

CD4+ T-cells expressing PD-1 and other immune checkpoints are enriched in HIV in HIV- infected individuals receiving cART (Banga et al., 2016; Chomont et al., 2009; Fromentin et al., 2016). Interestingly, these immune checkpoints appear to mediate their inhibitory activities through the metabolic reprogramming of the cells (Lim et al., 2017; Patsoukis et al., 2015). This suggests that the metabolic requirements of HIV-1 replication might enduringly imprint the infected cells.

Based on these finding, the invention provides compositions, methods and uses of a compound for inhibiting and treating viral and bacterial pathogen infections (e.g. HIV infections) and methods for assessing the effects of these compounds on pathogen infections. Compounds that inhibit the metabolic function cells can be used for inhibiting and treating pathogen infections in vitro and in vivo. In one embodiment, compounds that inhibit the metabolic function of CD4+ cells can be used for inhibiting and treating HIV infections in vitro and in vivo.

Screening Methods

The invention encompasses various screening methods for determining the effect of a compound that inhibits the metabolic function of cells in a pathogen-infected human and in vitro.

Within the scope of this invention, "metabolism” refers to chemical transformations within living cells that serve three main purposes: the conversion of food or fuel to energy to run cellular processes, the conversion of food or fuel to building blocks for proteins, lipids, nucleic acids and carbohydrates, and the elimination of waste.

Within the scope of this invention, "metabolic function" means a defined metabolism pathway, not including upstream or downstream effects. Preferably, the metabolic function(s) is(are) selected from those listed in Table 1 and/or Table 2.

Within the scope of this invention, "metabolic enzyme" means an enzyme within a defined metabolism pathway. More preferably, the targeted metabolic enzyme(s) is(are) selected from those listed in Table 1.

Within the scope of this invention, "metabolic inhibitor" means an inhibitor of metabolic process or enzyme within a defined metabolism pathway. Most preferably, the metabolic inhibitor(s) is(are) selected from those listed in Table 1. In preferred embodiments, the metabolic inhibitor (metabolic pathway/enzyme) is selected from l-DON (Glutaminolysis), 2DG (Glycolysis), etomoxir (fatty acid transport/ Carnitine palmitoyltransferase-1), UK5099 (mitochondrial pyruvate carrier (MPC)), BPTES (Glutaminolysis), Orlistat (fatty acid synthesis), Metformin (fatty acid oxydation), and Rapamycin (mTOR). Preferably, the metabolic inhibitor is active in human CD4+ cells and/or macrophages.

In the present invention, l-DON or DON refers to 6-Diazo-5-oxo-L-norleucine, also named as 6-diazo-5-oxo-l-norleucine or (L)-DON.

All possible combinations of 1, 2, 3, 4, or 5 of the metabolic inhibitors set forth herein (e.g., Table 1 & Table 2) are specifically contemplated. For example, preferred combinations of inhibitors are DON+2DG, DON+etomoxir+2DG, BPTES+UK5099, BPTES+Etomoxir, Etomoxir+UK5099, Etomoxir+2DG, and DON+2DG+ Etomoxir. In addition, all possible combinations of 1, 2, 3, 4, or 5 of the metabolic function targets and metabolic enzyme targets set forth herein (e.g., Table 1, Table 2, and the Examples) are specifically contemplated.

In one embodiment, the invention encompasses various screening methods for determining the effect of a metabolic inhibitor that inhibits the metabolic function of cells, preferably CD4+ T cells, in a human, preferably a Human Immunodeficiency Virus-infected human, and in vitro.

In one embodiment, the method comprises administering at least one dose of the metabolic inhibitor to a human or to cells in vitro; and measuring the level of pathogen (e.g., HIV) infection in the human or cells. Preferably, the metabolic inhibitor is active in human CD4+ cells.

In one embodiment, the method comprises administering at least one dose of the metabolic inhibitor to the human or cells; and measuring the level of plasma HIV RNA in the human or in the cell supernatant. In one embodiment, the method comprises administering at least one dose of the metabolic inhibitor to the human; and measuring the level of HIV-infected reservoir cells in the human.

In various embodiments, the metabolic inhibitor reduces glycolysis or blocks fatty acid transport to mitochondria. In a preferred embodiment, the metabolic inhibitor is administered a concentration that reduces the production of HIV from HIV-infected CD4+ T cells. In a preferred embodiment, the metabolic inhibitor is 2-deoxy-glucose (2-DG). Preferably, the cells are exposed to a concentration of 1-25 mM 2-DG, 3-10 mM 2-DG, or 5 mM 2-DG. In another preferred embodiment, the metabolic inhibitor is Etomoxir.

The metabolic inhibitor can be used by itself, or preferably, in combination with additional metabolic inhibitor, preferably one that affects a different metabolic function. Preferably, the metabolic inhibitor targets one or more of the metabolic functions or enzymes listed in Table 1 and/or Table 2. The metabolic inhibitors listed in Table 1 and Table 2, and modified versions thereof, are particularly preferred.

Table 1. Small molecules targeting cellular metabolism

Table 2. Overview of drugs, currently in clinical development (from van der Mijn et al. Cancer & Metabolism (2016) 4:14) The measurement can provide for a comparison to another infected individual that does not receive the metabolic inhibitor or to a prior measurement from that same infected individual, preferably before treatment with the metabolic inhibitor. Preferably, the measurement of the level of HIV infection in the human is performed at least twice. In some embodiments, the measurement is taken 3, 4, 5, 6, 7, 8, 9, or 10 times. In this way, the measurements can provide for a comparison over time within that infected individual, most preferably with a measurement taken before treatment with the metabolic inhibitor

For example, the level of HIV infection can be assessed by different techniques known to the skilled artisan. For example, the level of HIV infection in the human can be determined by measuring the level of plasma HIV RNA in the human.

For example, the level of plasma HIV RNA in the human can be measured by a reverse transcription and amplification reaction. For example, reverse transcription of the RNA of an HIV can be performed with a "reverse primer" specific for HIV. A "reverse primer" is one that, based on its 5'-3' orientation, can bind to a single-stranded RNA and serve to initiate generation of a complementary DNA (cDNA) copy of the RNA. The reverse transcription can be accomplished using well known and routine methods. The reaction mix for reverse transcription contains the reagents for the reaction, for example, a reverse primer, dNTPs (dATP, dCTP, dGTP and dTTP), a buffer, and a reverse transcriptase. Exemplary reaction conditions are set forth in the examples.

Amplification of the cDNA copy of an HIV generated by reverse transcription can be performed with a "forward primer" specific for HIV. A "forward primer" is one that, based on its 5'-3' orientation, can bind to a single-stranded antisense cDNA copy of an RNA generated by reverse transcription and serve to initiate generation of a double-stranded DNA copy of the RNA. The amplification can be accomplished using well known and routine methods. The reagent mix for amplification contains the reagents for the reaction, for example a forward primer, a reverse primer, dNTPs, a buffer, and a DNA polymerase.

In one embodiment, the method of the invention is performed using a single RT-PCR reagent mix containing the reagents for the reverse transcription and amplification reactions. Preferably, the reverse primer used for the reverse transcription reaction is also used for the amplification reaction. Preferably, the reverse transcription and amplification reactions are performed in a plastic or glass container, most preferably in the same container.

Amplification methods known in the art include RCA, MDA, NASBA, TMA, SDA, LCR, b- DNA, PCR (all forms including RT-PCR), RAM, LAMP, ICAN, SPIA, QB-replicase, or Invader. A preferred amplification method is the polymerase chain reaction (PCR) amplification. See, e.g., PCR Technology: Principles and Applications for DNA Amplification (Ed. H. A. Erlich, Freeman Press, NY, N.Y., 1992); PCR Protocols: A Guide to Methods and Applications (Eds. linis, et al., Academic Press, San Diego, Calif., 1990); Mattila et al., Nucleic Acids Res. 19, 4967 (1991); Eckert et al., PCR Methods and Applications 1, 17 (1991); PCR (Eds. McPherson et al., IRL Press, Oxford); and U.S. Pat. Nos. 4,683,202, 4,683,195, 4,800,159 4,965,188, and 5,333,675. More preferred PCR methods is real-time PCR, PCR-HRM (High-Resolution DNA Melting) (see Andriantsoanirina et al. Journal of Microbiological Methods, 78: 165 (2009)) and PCR coupled to ligase detection reaction based on fluorescent microsphere (Luminex® microspheres).

Amplification techniques include in particular isothermal methods and PCR-based techniques. Isothermal techniques include such methods as nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP), helicase-dependent amplification (HDA), rolling circle amplification (RCA), and strand displacement amplification (SDA), exponential amplification reaction (EXPAR), isothermal and chimeric primer-initiated amplification of nucleic acids (ICANs), signal-mediated amplification of RNA technology (SMART) and others (see e.g. Asiello and Baeumner, Lab Chip; 11(8): 1420-1430, 2011).

Preferably, the PCR technique quantitatively measures starting amounts of DNA, cDNA, or RNA. Examples of PCR-based techniques according to the invention include techniques such as, but not limited to, quantitative PCR (Q-PCR), reverse-transcriptase polymerase chain reaction (RT-PCR), quantitative reverse-transcriptase PCR (QRT-PCR), or digital PCR. These techniques are well known and easily available technologies for those skilled in the art.

Preferably, the method is a one-step real-time RT-PCR assay, for example, as described in the Examples. Most preferably, the method is a one-step real-time RT-PCR assay based on TAQMAN probe technology capable of detecting the recently described African E and F genogroups and including a competitive RNA internal control (1C), for example, as described in the Examples.

Preferably, a probe is used to detect the amplified product. The probe can be labeled with a fluorescent, radioactive, or enzymatic label. The amplified product can be detected with a specific detection chemistry such as fluorescence resonance energy transfer (FRET) probes, TAQMAN probes, molecular beacons, scorpion probes, fluorescently labeled (or other labeled) primers, lightup probes or a dye-based chemistry, DNA, PNA, LNA, or RNA including modified bases that bind to the amplified product to detect the sequence of interest.

Detection of the amplified products can be real-time (during the amplification process) or endpoint (after the amplification process). The invention allows for detection of the amplification products in the same vessel as amplification occurs.

Preferably, a DNA internal control is used to monitor the amplification reaction.

Preferably, a RNA internal control is used to monitor the reverse transcription and amplification reactions.

In some embodiments, the metabolic inhibitor (e.g., 2-DG) is administered in at least one administration of 1-200 mg/kg/day 5-160 mg/kg/day, 10-80 mg/kg/day, 20-70 mg/kg/day, 30-60 mg/kg/day, or 20-40 mg/kg/day. Preferably, the administration is at least 1-5, 5-10, 10-20, 20- 40, 40-60, 60-80, 80-100, 100-120, 120-140, or 140-160 mg/kg/day. Preferably, the administration is at least 1, 5, 10, 20, 40, 60, 80, 100, 120, 140, or 160 mg/kg/day of the metabolic inhibitor. Most preferably, the administration is at least 1, 5, 10, 20, 40, 60, 80, 100, 120, 140, or 160 mg/kg/day of 2-DG. Although not specifically enumerated, all values and subranges within the above and below ranges are specifically included as if explicitly written out. The administration of the metabolic inhibitor can be by many methods known in the art, most preferably intravenous, intra-arterial, subcutaneous, intramuscular, sublingual, transmucosal, or oral.

In some embodiments, multiple administrations are given. In various embodiments, at least 1-100, preferably 1, 2, 3, 4, 5, 6, 7, 8, 9, 10-20, 20-50, or 50-100, administrations are given. In various embodiments, the administration is at least twice/ day, twice/week, once/day, once/week, three times/week, or once/every 2 days. The administration can be given continuously (e.g., with a pump).

In various embodiments, at least .01, .05, 0.1, 0.5, 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 100, 120, 160 mg/kg/day of a metabolic inhibitor is administered for at least 1, 2, 3, 4, 5, 6, 7 days, 1, 2, 3, 4, 5, 6 weeks, or 1, 2, 3, 4, 5, 6, etc. months.

In various embodiments, at least .01, .05, 0.1, 0.5, 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 100, 120, 160 mg/kg/day is administered every 2 days or 3 times/week for at least 2, 3, 4, 5, 6, 7 days, 1, 2, 3, 4, 5, 6 weeks, or 1, 2, 3, 4, 5, 6, etc. months.

Pathogens

Pathogens within the scope of the invention include:

Acinetobacter baumannii, Anaplasma genus, Anaplasma phagocytophilum, Ancylostoma braziliense, Ancylostoma duodenale, Arcanobacterium haemolyticum, Ascaris lumbricoides, Aspergillus genus, Astroviridae, Babesia genus, Bacillus anthracis, Bacillus cereus, Bartonella henselae, BK virus, Blastocystis hominis, Blastomyces dermatitidis, Bordetella pertussis, Borrelia burgdorferi, Borrelia genus, Borrelia spp, Brucella genus, Brugia malayi, Bunyaviridae family, Burkholderia cepacia and other Burkholderia species, Burkholderia mallei, Burkholderia pseudomallei, Caliciviridae family, Campylobacter genus, Candida albicans, Candida spp, Chlamydia trachomatis, Chlamydophila pneumoniae, Chlamydophila psittaci, CJD prion, Clonorchis sinensis, Clostridium botulinum, Clostridium difficile, Clostridium perfringens, Clostridium perfringens, Clostridium spp, Clostridium tetani, Coccidioides spp, coronaviruses, Corynebacterium diphtheriae, Coxiella burnetii, Crimean-Congo hemorrhagic fever virus, Cryptococcus neoformans, Cryptosporidium genus, Cytomegalovirus (CMV), Dengue viruses (DEN-1, DEN-2, DEN-3 and DEN-4), Dientamoeba fragilis, Ebolavirus (EBOV), Echinococcus genus, Ehrlichia chaffeensis, Ehrlichia ewingii, Ehrlichia genus, Entamoeba histolytica, Enterococcus genus, Enterovirus genus, Enteroviruses, mainly Coxsackie A virus and Enterovirus 71 (EV71), Epidermophyton spp, Epstein-Barr Virus (EBV), Escherichia coli 0157:1-17, 0111 and 0104:1-14, Fasciola hepatica and Fasciola gigantica, FFI prion, Filarioidea superfamily, Flaviviruses, Francisella tularensis, Fusobacterium genus, Geotrichum candidum, Giardia intestinalis, Gnathostoma spp, GSS prion, Guanarito virus, Haemophilus ducreyi, Haemophilus influenzae, Helicobacter pylori, Henipavirus (Hendra virus Nipah virus), Hepatitis A Virus, Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), Hepatitis D Virus, Hepatitis E Virus, Herpes simplex virus 1 and 2 (HSV-1 and HSV-2), Histoplasma capsulatum, HIV (Human immunodeficiency virus, particularly HIV-1 and HIV-2), Hortaea werneckii, Human bocavirus (HBoV), Human herpesvirus 6 (HHV-6) and Human herpesvirus 7 (HHV-7), Human metapneumovirus (hMPV), Human papillomavirus (HPV), Human parainfluenza viruses (HPIV), Japanese encephalitis virus, JC virus, Junin virus, Kingella kingae, Klebsiella granulomatis, Kuru prion, Lassa virus, Legionella pneumophila, Leishmania genus, Leptospira genus, Listeria monocytogenes, Lymphocytic choriomeningitis virus (LCMV), Machupo virus, Malassezia spp, Marburg virus, Measles virus, Metagonimus yokagawai, Microsporidia phylum, Molluscum contagiosum virus (MCV), Mumps virus, Mycobacterium leprae and Mycobacterium lepromatosis, Mycobacterium tuberculosis, Mycobacterium ulcerans, Mycoplasma pneumoniae, Naegleria fowleri, Necator americanus, Neisseria gonorrhoeae, Neisseria meningitidis, Nocardia asteroides, Nocardia spp, Onchocerca volvulus, Orientia tsutsugamushi, Orthomyxoviridae family (Influenza), Paracoccidioides brasiliensis, Paragonimus spp, Paragonimus westermani, Parvovirus B19, Pasteurella genus, Plasmodium genus, Pneumocystis jirovecii, Poliovirus, Rabies virus, Respiratory syncytial virus (RSV), Rhinovirus, rhinoviruses, Rickettsia akari, Rickettsia genus, Rickettsia prowazekii, Rickettsia rickettsii, Rickettsia typhi, Rift Valley fever virus, Rotavirus, Rubella virus, Sabia virus, Salmonella genus, Sarcoptes scabiei, SARS coronavirus, Schistosoma genus, Shigella genus, Sin Nombre virus, Hantavirus, Sporothrix schenckii, Staphylococcus genus, Staphylococcus genus, Streptococcus agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, Strongyloides stercoralis, Taenia genus, Taenia solium, Tick-borne encephalitis virus (TBEV), Toxocara canis or Toxocara cati, Toxoplasma gondii, Treponema pallidum, Trichinella spiralis, Trichomonas vaginalis, Trichophyton spp, Trichuris trichiura, Trypanosoma brucei, Trypanosoma cruzi, Ureaplasma urealyticum, Varicella zoster virus (VZV), Varicella zoster virus (VZV), Variola major or Variola minor, vCJD prion, Venezuelan equine encephalitis virus, Vibrio cholerae, West Nile virus, Western equine encephalitis virus, Wuchereria bancrofti, Yellow fever virus, Yersinia enterocolitica, Yersinia pestis, Yersinia pseudotuberculosis, and Zika virus. Treatment Methods and Uses

The invention encompasses methods of treatment using the metabolic inhibitors described above and the use of compositions comprising these metabolic inhibitors in the treatment of a pathogen infection in a human patient.

In one embodiment, the method comprises administering at least one metabolic inhibitor to a pathogen-infected human at a concentration that reduces the production of the pathogen from said pathogen-infected cells. In one embodiment, the method comprises administering at least 2 metabolic inhibitors of different metabolic functions in the cells at concentrations that reduce the production of the pathogen from said pathogen-infected cells.

Preferably, a suboptimal dosage of the metabolic inhibitor is administered. Within the scope of this invention, the term "suboptimal" refers to a dose at which the metabolic inhibitor does not have on its own maximum effects either on the metabolic pathways, but also do not cause more than 10% cell death of the corresponding uninfected cells (e.g. uninfected CD4+ T cells).

In one embodiment, the method comprises administering an effective amount of at least one metabolic inhibitor to an HIV-infected human. An effective amount is an amount of the metabolic inhibitor(s) that reduces the level of detectable pathogen (e.g., plasma HIV RNA in an HIV-infected patient) at least 2-fold. In some embodiments, the administration of metabolic inhibitor(s) reduces the level of detectable pathogen (e.g., plasma HIV RNA in an HIV-infected patient) at least 2-, 4-, 10-, 30-, 50-, or 100-fold.

In some embodiments, the administration of the metabolic inhibitor reduces the pathogen (e.g., viral or bacterial) load in the patient at least 2-, 4-, 10-, 30-, 50-, or 100-fold. In some embodiments, the administration of the metabolic inhibitor reduces the number of HIV-1 infected reservoir cells at least 2-, 4-, 10-, 30-, 50-, or 100-fold. In some embodiments, the administration of the metabolic inhibitor reduces active viral or bacterial replication at least 2-, 4-, 10-, 30-, 50-, or 100-fold. .

The above reductions can be determined by routine techniques in the art, such as by comparing the levels in the patient before and after administration of the metabolic inhibitor, for example by standard PCR amplification methods with patient plasma samples. The reduction can be assessed at various times after administration, for example at 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 16, 20, 40, or 52 weeks after administration of the metabolic inhibitor.

In some embodiments, the metabolic inhibitor is administered in at least one administration of 1-200 mg, 5-160 mg, 10-80 mg, or 20-40 mg. Preferably, the administration is at least .01-0.1, 0.1-1, 1-5, 5-10, 10-20, 20-40, 40-60, 60-80, 80-100, 100-120, 120-140, or 140- 160 mg. Preferably, the administration is at least .01, .05, 0.1, 0.5, 1, 5, 10, 20, 40, 60, 80, 100, 120, 140, or 160 mg of the metabolic inhibitor. Although not specifically enumerated, all values and subranges within the above and below ranges are specifically included as if explicitly written out.

The administration of the metabolic inhibitor can be by many methods known in the art, most preferably intravenous, intra-arterial, subcutaneous, intramuscular, sublingual, transmucosal, or oral.

In some embodiments, multiple administrations are given. In various embodiments, at least 1-100, preferably 1, 2, 3, 4, 5, 6, 7, 8, 9, 10-20, 20-50, or 50-100, administrations are given. In various embodiments, the administration is at least twice/ day, twice/week, once/day, once/week, three times/week, or once/every 2 days.

In various embodiments, at least .01, .05, 0.1, 0.5, 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 100, 120, 160 mg/kg/day is administered for at least 1, 2, 3, 4, 5, 6, 7 days, 1, 2, 3, 4, 5, 6 weeks, or 1, 2, 3, 4, 5, 6, etc. months.

In various embodiments, at least 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 100, 120, 160 mg is administered every 2 days or 3 times/week for at least 2, 3, 4, 5, 6, 7 days, 1, 2, 3, 4, 5, 6 weeks, or 1, 2, 3, 4, 5, 6, etc. months.

HIV-infected Patients

The methods, uses, and compositions of this invention can be used with HIV-infected patients. In one embodiment, the patient is infected with Human Immunodeficiency Virus type

1 (HIV-1). In one embodiment, the patient is infected with Human Immunodeficiency Virus type

2 (HIV-2). In one embodiment, the HIV-1 infected patient is acutely infected with HIV. In one embodiment, the HIV-1 infected patient is chronically infected with HIV.

In one embodiment, the HIV-1 infected patient is undergoing cART. In one embodiment, the HIV-1 infected patient has never initiated cART. In various embodiments, the HIV-1 infected patient has previously undergone cART, and either ceases or continues cART.

Pharmaceutical Compositions

The invention encompasses pharmaceutical compositions comprising one or more metabolic inhibitors. The compositions are preferably for the treatment of a pathogen infection in a human, particularly, preferably comprising a combination of at least two, three, or four metabolic inhibitors. The invention further encompasses the use of these compositions in the manufacture of a medicament for the treatment of a pathogen infection and the use of these compositions in the treatment of a pathogen infection.

In various embodiments, the composition contains 1-200 mg, 5-160 mg, 10-80 mg, or 20- 40 mg of one or more metabolic inhibitor. Preferably, the composition contains at least 1-5, 5- 10, 10-20, 20-40, 40-60, 60-80, 80-100, 100-120, 120-140, or 140-160 mg of one or more metabolic inhibitor. Although not specifically enumerated, all values and subranges within the above ranges are specifically included as if explicitly written out.

The metabolic inhibitors may also be advantageously administered for therapeutic purposes together with other metabolic inhibitors, such as HIV inhibitors, particularly cART, known in the general art to be of value in treating HIV infection. Particularly preferred combinations contain at least one, two, three, or four of the HIV inhibitors listed below. Most preferably, the combination contains at least one of the combination antiretroviral therapies listed below.

Effective concentrations or amounts of a metabolic inhibitor can be mixed with a suitable pharmaceutical carrier or vehicle for systemic, topical or local administration to form pharmaceutical compositions. The metabolic inhibitor is included in an amount effective for treating the pathogen (e.g., HIV) infection. The concentration of active agent in the composition will depend on absorption, inactivation, excretion rates of the active agent, the dosage schedule, amount administered, particular formulation as well as other factors known to those of skill in the art.

The compositions are intended to be administered by a suitable route, including by way of example and without limitation orally, parenterally, rectally, topically and locally. For oral administration, capsules and tablets can be used. The compositions are in liquid, semi-liquid or solid foul and are formulated in a manner suitable for each route of administration.

Solutions or suspensions used for parenteral, intradermal, subcutaneous, or topical application can include any of the following components, in any combination: a sterile diluent, including by way of example without limitation, water for injection, saline solution, fixed oil, polyethylene glycol, glycerine, propylene glycol or other synthetic solvent; antimicrobial agents, such as benzyl alcohol and methyl parabens; antioxidants, such as ascorbic acid and sodium bisulfite; chelating agents, such as ethylenediaminetetraacetic acid (EDTA); buffers, such as acetates, citrates and phosphates; and agents for the adjustment of tonicity such as sodium chloride or dextrose. Parenteral preparations can be enclosed in ampoules, disposable syringes or single or multiple dose vials made of glass, plastic or other suitable material.

In instances in which the agents exhibit insufficient solubility, methods for solubilizing agents may be used. Such methods are known to those of skill in this art, and include, but are not limited to, using co-solvents, such as dimethylsulfoxide (DMSO), using surfactants, such as TWEEN®, or dissolution in aqueous sodium bicarbonate. Pharmaceutically acceptable derivatives of the agents may also be used in formulating effective pharmaceutical compositions.

Upon mixing or addition of the agent(s), the resulting mixture may be a solution, suspension, emulsion or the like. The form of the resulting mixture depends upon a number of factors, including the intended mode of administration and the solubility of the agent in the selected carrier or vehicle. The effective concentration is sufficient for treating one or more symptoms of at least one disease state.

The pharmaceutical compositions are provided for administration to humans and animals in unit dosage forms, such as tablets, capsules, pills, powders, granules, sterile parenteral solutions or suspensions, and oral solutions or suspensions, and oil-water emulsions containing suitable quantities of the agents or pharmaceutically acceptable derivatives thereof. The pharmaceutically therapeutically active agents and derivatives thereof are typically formulated and administered in unit-dosage forms or multiple-dosage forms. Unit-dose foams as used herein refers to physically discrete units suitable for human and animal subjects and packaged individually as is known in the art. Each unit-dose contains a predetermined quantity of the therapeutically active agent sufficient to produce the desired therapeutic effect, in association with the required pharmaceutical carrier, vehicle or diluent. Examples of unit-dose forms include ampoules and syringes and individually packaged tablets or capsules. Unit-dose forms may be administered in fractions or multiples thereof. A multiple-dose form is a plurality of identical unit-dosage forms packaged in a single container to be administered in segregated unit-dose form. Examples of multiple-dose forms include vials, bottles of tablets or capsules or bottles of pints or gallons. Hence, multiple dose form is a multiple of unit-doses which are not segregated in packaging.

The composition can contain along with the active agent, for example and without limitation: a diluent such as lactose, sucrose, dicalcium phosphate, or carboxymethylcellulose; a lubricant, such as magnesium stearate, calcium stearate and talc; and a binder such as starch, natural gums, such as gum acacia gelatin, glucose, molasses, polyvinylpyrrolidone, celluloses and derivatives thereof, povidone, crospovidones and other such binders known to those of skill in the art. Liquid pharmaceutically administrable compositions can, for example, be prepared by dissolving, dispersing, or otherwise mixing an active agent as defined above and optional pharmaceutical adjuvants in a carrier, such as, by way of example and without limitation, water, saline, aqueous dextrose, glycerol, glycols, ethanol, and the like, to thereby form a solution or suspension. If desired, the pharmaceutical composition to be administered may also contain minor amounts of nontoxic auxiliary substances such as wetting agents, emulsifying agents, or solubilizing agents, pH buffering agents and the like, such as, by way of example and without limitation, acetate, sodium citrate, cyclodextrin derivatives, sorbitan monolaurate, triethanolamine sodium acetate, triethanolamine oleate, and other such agents. Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in this art; for example, see Remington's Pharmaceutical Sciences, Mack Publishing Company, Easton, Pa., 15th Edition, 1975. The composition or formulation to be administered will, in any event, contain a quantity of the active agent in an amount sufficient to alleviate the symptoms of the treated subject.

Dosage forms or compositions containing active agent in the range of 0.005% to 100% with the balance made up from non-toxic carrier may be prepared. For oral administration, a pharmaceutically acceptable non-toxic composition is formed by the incorporation of any of the normally employed excipients, such as, for example and without limitation, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, talcum, cellulose derivatives, sodium crosscarmellose, glucose, sucrose, magnesium carbonate or sodium saccharin. Such compositions include solutions, suspensions, tablets, capsules, powders and sustained release formulations, such as, but not limited to, implants and microencapsulated delivery systems, and biodegradable, biocompatible polymers, such as collagen, ethylene vinyl acetate, polyanhydrides, polyglycolic acid, polyorthoesters, polylactic acid and others. Methods for preparation of these compositions are known to those skilled in the art. The contemplated compositions may contain 0.001%-100% active agent, such as 0.1-85%, or such as 75-95%. The active agents or pharmaceutically acceptable derivatives may be prepared with carriers that protect the agent against rapid elimination from the body, such as time release formulations or coatings. The compositions may include other active agents to obtain desired combinations of properties.

Oral pharmaceutical dosage forms include, by way of example and without limitation, solid, gel and liquid. Solid dosage forms include tablets, capsules, granules, and bulk powders. Oral tablets include compressed, chewable lozenges and tablets which may be enteric-coated, sugar-coated or film-coated. Capsules may be hard or soft gelatin capsules, while granules and powders may be provided in non-effervescent or effervescent forms with the combination of other ingredients known to those skilled in the art.

In some embodiments, the formulations are solid dosage forms, such as capsules or tablets. The tablets, pills, capsules, troches and the like can contain any of the following ingredients, or agents of a similar nature: a binder; a diluent; a disintegrating agent; a lubricant; a glidant; a sweetening agent; and a flavoring agent. Examples of binders include, by way of example and without limitation, microcrystalline cellulose, gum tragacanth, glucose solution, acacia mucilage, gelatin solution, sucrose, and starch paste. Lubricants include, by way of example and without limitation, talc, starch, magnesium or calcium stearate, lycopodium and stearic acid. Diluents include, by way of example and without limitation, lactose, sucrose, starch, kaolin, salt, mannitol, and dicalcium phosphate. Glidants include, by way of example and without limitation, colloidal silicon dioxide. Disintegrating agents include, by way of example and without limitation, crosscarmellose sodium, sodium starch glycolate, alginic acid, corn starch, potato starch, bentonite, methylcellulose, agar and carboxymethylcellulose. Coloring agents include, by way of example and without limitation, any of the approved certified water soluble FI) and C dyes, mixtures thereof; and water insoluble ID and C dyes suspended on alumina hydrate. Sweetening agents include, by way of example and without limitation, sucrose, lactose, mannitol and artificial sweetening agents such as saccharin, and any number of spray dried flavors. Flavoring agents include, by way of example and without limitation, natural flavors extracted from plants such as fruits and synthetic blends of agents which produce a pleasant sensation, such as, but not limited to peppermint and methyl salicylate. Wetting agents include, by way of example and without limitation, propylene glycol monostearate, sorbitan monooleate, diethylene glycol monolaurate, and polyoxyethylene laural ether. Emetic-coatings include, by way of example and without limitation, fatty acids, fats, waxes, shellac, ammoniated shellac and cellulose acetate phthalates. Film coatings include, by way of example and without limitation, hydroxyethylcellulose, sodium carboxymethylcellulose, polyethylene glycol 4000 and cellulose acetate phthalate.

If oral administration is desired, the agent could be provided in a composition that protects it from the acidic environment of the stomach. For example, the composition can be formulated in an enteric coating that maintains its integrity in the stomach and releases the active agent in the intestine. The composition may also be formulated in combination with an antacid or other such ingredient.

When the dosage unit form is a capsule, it can contain, in addition to material of the above type, a liquid carrier such as a fatty oil. In addition, dosage unit forms can contain various other materials which modify the physical form of the dosage unit, for example, coatings of sugar and other enteric agents. The agents can also be administered as a component of an elixir, suspension, syrup, wafer, sprinkle, chewing gum or the like. A syrup may contain, in addition to the active agents, sucrose as a sweetening agent and certain preservatives, dyes and colorings and flavors.

The active materials can also be mixed with other active materials which do not impair the desired action, or with materials that supplement the desired action, such as antacids, H2 blockers, and diuretics.

Pharmaceutically acceptable carriers included in tablets are binders, lubricants, diluents, disintegrating agents, coloring agents, flavoring agents, and wetting agents. Enteric-coated tablets, because of the enteric-coating, resist the action of stomach acid and dissolve or disintegrate in the neutral or alkaline intestines. Sugar-coated tablets are compressed tablets to which different layers of pharmaceutically acceptable substances are applied. Film-coated tablets are compressed tablets which have been coated with a polymer or other suitable coating. Multiple compressed tablets are compressed tablets made by more than one compression cycle utilizing the pharmaceutically acceptable substances previously mentioned. Coloring agents may also be used in the above dosage forms. Flavoring and sweetening agents are used in compressed tablets, sugar-coated, multiple compressed and chewable tablets. Flavoring and sweetening agents are useful in the formation of chewable tablets and lozenges.

Liquid oral dosage forms include aqueous solutions, emulsions, suspensions, solutions and/or suspensions reconstituted from non-effervescent granules and effervescent preparations reconstituted from effervescent granules. Aqueous solutions include, for example, elixirs and syrups. Emulsions are either oil-in-water or water-in-oil.

Elixirs are clear, sweetened, hydroalcoholic preparations. Pharmaceutically acceptable carriers used in elixirs include solvents. Syrups are concentrated aqueous solutions of a sugar, for example, sucrose, and may contain a preservative. An emulsion is a two-phase system in which one liquid is dispersed in the form of small globules throughout another liquid. Pharmaceutically acceptable carriers used in emulsions are non-aqueous liquids, emulsifying agents and preservatives. Suspensions use pharmaceutically acceptable suspending agents and preservatives. Pharmaceutically acceptable substances used in non-effervescent granules, to be reconstituted into a liquid oral dosage form, include diluents, sweeteners and wetting agents. Pharmaceutically acceptable substances used in effervescent granules, to be reconstituted into a liquid oral dosage form, include organic acids and a source of carbon dioxide. Coloring and flavoring agents may be used in any of the above dosage forms.

Solvents, include by way of example and without limitation, glycerin, sorbitol, ethyl alcohol and syrup. Examples of preservatives include without limitation glycerin, methyl and propylparaben, benzoic add, sodium benzoate and alcohol. Non-aqueous liquids utilized in emulsions, include by way of example and without limitation, mineral oil and cottonseed oil. Emulsifying agents, include by way of example and without limitation, gelatin, acacia, tragacanth, bentonite, and surfactants such as polyoxyethylene sorbitan monooleate. Suspending agents include, by way of example and without limitation, sodium carboxymethylcellulose, pectin, tragacanth, Veegum and acacia. Diluents include, by way of example and without limitation, lactose and sucrose. Sweetening agents include, by way of example and without limitation, sucrose, syrups, glycerin and artificial sweetening agents such as saccharin. Wetting agents, include by way of example and without limitation, propylene glycol monostearate, sorbitan monooleate, diethylene glycol monolaurate, and polyoxyethylene lauryl ether. Organic acids include, by way of example and without limitation, citric and tartaric acid. Sources of carbon dioxide include, by way of example and without limitation, sodium bicarbonate and sodium carbonate. Coloring agents include, by way of example and without limitation, any of the approved certified water soluble FD and C dyes, and mixtures thereof. Flavoring agents include, by way of example and without limitation, natural flavors extracted from plants such fruits, and synthetic blends of agents which produce a pleasant taste sensation.

For a solid dosage form, the solution or suspension, in for example propylene carbonate, vegetable oils or triglycerides, is encapsulated in a gelatin capsule. Such solutions, and the preparation and encapsulation thereof, are disclosed in U.S. Patent Nos. 4,328,245; 4,409,239; and 4,410,545. For a liquid dosage form, the solution, for example in a polyethylene glycol, may be diluted with a sufficient quantity of a pharmaceutically acceptable liquid carrier, e.g., water, to be easily measured for administration.

Alternatively, liquid or semi-solid oral formulations may be prepared by dissolving or dispersing the active agent or salt in vegetable oils, glycols, triglycerides, propylene glycol esters (e.g., propylene carbonate) and other such carriers, and encapsulating these solutions or suspensions in hard or soft gelatin capsule shells. Other useful formulations include those set forth in U.S. Patent Nos. Re 28,819 and 4,358,603. Briefly, such formulations include, but are not limited to, those containing an agent provided herein, a dial kylated mono- or poly-alkylene glycol, including, but not limited to, 1,2-dimethoxymethane, diglyme, triglyme, tetraglyme, polyethylene glycol-350-dimethyl ether, polyethylene glycol-550-dimethyl ether, polyethylene glycol-750-dimethyl ether wherein 350, 550 and 750 refer to the approximate average molecular weight of the polyethylene glycol, and one or more antioxidants, such as butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), propyl gallate, vitamin E, hydroquinone, hydroxycoumarins, ethanolamine, lecithin, cephalin, ascorbic acid, malic acid, sorbitol, phosphoric acid, thiodipropionic acid and its esters, and dithiocarbamates.

Other formulations include, but are not limited to, aqueous alcoholic solutions including a pharmaceutically acceptable acetal. Alcohols used in these formulations are any pharmaceutically acceptable water-miscible solvents having one or more hydroxyl groups, including, but not limited to, propylene glycol and ethanol. Acetals include, but are not limited to, di(lower alkyl) acetals of lower alkyl aldehydes such as acetaldehyde diethyl acetal.

Tablets and capsules formulations may be coated as known by those of skill in the art in order to modify or sustain dissolution of the active ingredient. Thus, for example and without limitation, they may be coated with a conventional enterically digestible coating, such as phenylsalicylate, waxes and cellulose acetate phthalate.

Parenteral administration, generally characterized by injection, either subcutaneously, intramuscularly or intravenously is also contemplated herein. Injectables can be prepared in conventional forms, either as liquid solutions or suspensions, solid forms suitable for solution or suspension in liquid prior to injection, or as emulsions. Suitable excipients, include by way of example and without limitation, water, saline, dextrose, glycerol or ethanol. In addition, if desired, the pharmaceutical compositions to be administered may also contain minor amounts of non-toxic auxiliary substances such as wetting or emulsifying agents, pH buffering agents, stabilizers, solubility enhancers, and other such agents, such as for example, sodium acetate, sorbitan monolaurate, triethanolamine oleate and cyclodextrins.

Implantation of a slow-release or sustained-release system, such that a constant level of dosage is maintained (see, e.g., U.S. Patent No. 3,710,795) is also contemplated herein. Briefly, the metabolic inhibitor can be dispersed in a solid inner matrix, e.g., polymethylmethacrylate, polybutylmethacrylate, plasticized or unplasticized polyvinylchloride, plasticized nylon, plasticized polyethyleneterephthalate, natural rubber, polyisoprene, polyisobutylene, polybutadiene, polyethylene, ethylene-vinylacetate copolymers, silicone rubbers, polydimethylsiloxanes, silicone carbonate copolymers, hydrophilic polymers such as hydrogels of esters of acrylic and methacrylic acid, collagen, cross-linked polyvinylalcohol and cross-linked partially hydrolyzed polyvinyl acetate, that is surrounded by an outer polymeric membrane, e.g., polyethylene, polypropylene, ethylene/propylene copolymers, ethylene/ethyl acrylate copolymers, ethylene/vinylacetate copolymers, silicone rubbers, polydimethyl siloxanes, neoprene rubber, chlorinated polyethylene, polyvinylchloride, vinylchloride copolymers with vinyl acetate, vinylidene chloride, ethylene and propylene, ionomer polyethylene terephthalate, butyl rubber epichlorohydrin rubbers, ethylene/vinyl alcohol copolymer, ethylene/vinyl acetate/vinyl alcohol terpolymer, and ethylene/vinyloxyethanol copolymer, that is insoluble in body fluids. The agent diffuses through the outer polymeric membrane in a release rate controlling step. The percentage of active agent contained in such parenteral compositions is highly dependent on the specific nature thereof, as well as the activity of the agent and the needs of the subject.

Parenteral administration includes intravenous, subcutaneous and intramuscular administrations. Preparations for parenteral administration include sterile solutions ready for injection, sterile dry soluble products, such as lyophilized powders, ready to be combined with a solvent just prior to use, including hypodermic tablets, sterile suspensions ready for injection, sterile dry insoluble products ready to be combined with a vehicle just prior to use and sterile emulsions. The solutions may be either aqueous or nonaqueous. If administered intravenously, suitable carriers include physiological saline or phosphate buffered saline (PBS), and solutions containing thickening and solubilizing agents, such as glucose, polyethylene glycol, and polypropylene glycol and mixtures thereof.

Pharmaceutically acceptable carriers used in parenteral preparations include aqueous vehicles, nonaqueous vehicles, antimicrobial agents, isotonic agents, buffers, antioxidants, local anesthetics, suspending and dispersing agents, emulsifying agents, sequestering or chelating agents and other pharmaceutically acceptable substances.

Aqueous vehicles include, by way of example and without limitation, Sodium Chloride Injection, Ringers Injection, Isotonic Dextrose Injection, Sterile Water Injection, Dextrose and Lactated Ringers Injection. Nonaqueous parenteral vehicles include, by way of example and without limitation, fixed oils of vegetable origin, cottonseed oil, corn oil, sesame oil and peanut oil. Antimicrobial agents in bacteriostatic or fungistatic concentrations must be added to parenteral preparations packaged in multiple-dose containers which include phenols or cresols, mercurials, benzyl alcohol, chlorobutanol, methyl and propyl p-hydroxybenzoic acid esters, thimerosal, benzalkonium chloride and benzethonium chloride. Isotonic agents include, by way of example and without limitation, sodium chloride and dextrose. Buffers include phosphate and citrate. Antioxidants include sodium bisulfate. Local anesthetics include procaine hydrochloride. Suspending and dispersing agents include sodium carboxymethylcelluose, hydroxypropyl methylcellulose and polyvinylpyrrolidone. Emulsifying agents include Polysorbate 80 (TWEEN® 80). A sequestering or chelating agent of metal ions include EDTA. Pharmaceutical carriers also include, by way of example and without limitation, ethyl alcohol, polyethylene glycol and propylene glycol for water miscible vehicles and sodium hydroxide, hydrochloric acid, citric acid or lactic acid for pH adjustment.

The concentration of the pharmaceutically active agent is adjusted so that an injection provides an effective amount to produce the desired pharmacological effect. The exact dose depends on the age, weight and condition of the patient or animal as is known in the art.

The unit-dose parenteral preparations are packaged in an ampoule, a vial or a syringe with a needle. Preparations for parenteral administration should be sterile, as is known and practiced in the art. Illustratively, intravenous or intra-arterial infusion of a sterile aqueous solution containing an active agent is an effective mode of administration. Another embodiment is a sterile aqueous or oily solution or suspension containing an active agent injected as necessary to produce the desired pharmacological effect.

Injectables are designed for local and systemic administration. Typically a therapeutically effective dosage is formulated to contain a concentration of at least about 0.1% w/w up to about 90% w/w or more, such as more than 1% w/w of the active agent to the treated tissue(s). The active agent may be administered at once, or may be divided into a number of smaller doses to be administered at intervals of time. It is understood that the precise dosage and duration of treatment is a function of the tissue being treated and may be determined empirically using known testing protocols or by extrapolation from in vivo or in vitro test data. It is to be noted that concentrations and dosage values may also vary with the age of the individual treated. It is to be further understood that for any particular subject, specific dosage regimens should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the formulations, and that the concentration ranges set forth herein are exemplary only and are not intended to limit the scope or practice of the claimed formulations.

The agent may be suspended in micronized or other suitable form or may be derivatized, e.g., to produce a more soluble active product or to produce a prodrug or other pharmaceutically acceptable derivative. The form of the resulting mixture depends upon a number of factors, including the intended mode of administration and the solubility of the agent in the selected carrier or vehicle. The effective concentration is sufficient for ameliorating the symptoms of the condition and may be empirically determined.

Lyophilized powders can be reconstituted for administration as solutions, emulsions, and other mixtures or formulated as solids or gels.

The sterile, lyophilized powder is prepared by dissolving an agent provided herein, or a pharmaceutically acceptable derivative thereof, in a suitable solvent. The solvent may contain an excipient which improves the stability or other pharmacological component of the powder or reconstituted solution, prepared from the powder. Excipients that may be used include, but are not limited to, dextrose, sorbital, fructose, corn syrup, xylitol, glycerin, glucose, sucrose or other suitable agent. The solvent may also contain a buffer, such as citrate, sodium or potassium phosphate or other such buffer known to those of skill in the art at, typically, about neutral pH. Subsequent sterile filtration of the solution followed by lyophilization under standard conditions known to those of skill in the art provides the desired formulation. Generally, the resulting solution will be apportioned into vials for lyophilization. Each vial will contain, by way of example and without limitation, a single dosage (10-1000 mg, such as 100-500 mg) or multiple dosages of the agent. The lyophilized powder can be stored under appropriate conditions, such as at about 4°C to room temperature.

Reconstitution of this lyophilized powder with water for injection provides a formulation for use in parenteral administration. For reconstitution, about 1-50 mg, such as about 5-35 mg, for example, about 9-30 mg of lyophilized powder, is added per mL of sterile water or other suitable carrier. The precise amount depends upon the selected agent. Such amount can be empirically determined.

Topical mixtures are prepared as described for the local and systemic administration. The resulting mixture may be a solution, suspension, emulsions or the like and are formulated as creams, gels, ointments, emulsions, solutions, elixirs, lotions, suspensions, tinctures, pastes, foams, aerosols, irrigations, sprays, suppositories, bandages, dermal patches or any other formulations suitable for topical administration.

The agents or pharmaceutically acceptable derivatives thereof may be formulated as aerosols for topical application, such as by inhalation (see, e.g., U.S. Patent Nos. 4,044,126, 4,414,209, and 4,364,923, which describe aerosols for delivery of a steroid useful for treatment of inflammatory diseases, particularly asthma). These formulations for administration to the respiratory tract can be in the form of an aerosol or solution for a nebulizer, or as a microfine powder for insufflation, alone or in combination with an inert carrier such as lactose. In such a case, the particles of the formulation will, by way of example and without limitation, have diameters of less than about 50 microns, such as less than about 10 microns.

The agents may be formulated for local or topical application, such as for topical application to the skin and mucous membranes, such as in the eye, in the form of gels, creams, and lotions and for application to the eye or for intracisternal or intraspinal application. Topical administration is contemplated for transdermal delivery and also for administration to the eyes or mucosa, or for inhalation therapies. Nasal solutions of the active agent alone or in combination with other pharmaceutically acceptable excipients can also be administered.

These solutions, particularly those intended for ophthalmic use, may be formulated, by way of example and without limitation, as about 0.01% to about 10% isotonic solutions, pH about 5-7, with appropriate salts.

Other routes of administration, such as transdermal patches, and rectal administration are also contemplated herein.

Transdermal patches, including iotophoretic and electrophoretic devices, are well known to those of skill in the art. For example, such patches are disclosed in U.S. Patent Nos.

6,267,983, 6,261,595, 6,256,533, 6,167,301, 6,024,975, 6,010715, 5,985,317, 5,983,134,

5,948,433, and 5,860,957.

Pharmaceutical dosage forms for rectal administration are rectal suppositories, capsules and tablets for systemic effect. Rectal suppositories are used herein mean solid bodies for insertion into the rectum which melt or soften at body temperature releasing one or more pharmacologically or therapeutically active ingredients. Pharmaceutically acceptable substances utilized in rectal suppositories are bases or vehicles and agents to raise the melting point. Examples of bases include cocoa butter (theobroma oil), glycerin-gelatin, carbowax (polyoxyethylene glycol) and appropriate mixtures of mono-, di- and triglycerides of fatty acids. Combinations of the various bases may be used. Agents to raise the melting point of suppositories include spermaceti and wax. Rectal suppositories may be prepared either by the compressed method or by molding. The typical weight of a rectal suppository is, by way of example and without limitation, about 2 to 3 gm.

Tablets and capsules for rectal administration are manufactured using the same pharmaceutically acceptable substance and by the same methods as for formulations for oral administration.

Kit of Parts The invention includes a kit of parts for simultaneous, separate, sequential administration to a pathogen-infected patient. The kit can comprise any combination of the metabolic inhibitors of the invention in pharmaceutical compositions together.

The kit of parts can contain at least 1-200 mg, 5-160 mg, 10-80 mg, or 20-40 mg of two or more metabolic inhibitors. Preferably, the kit of parts contains at least 1-5, 5-10, 10-20, 20- 40, 40-60, 60-80, 80-100, 100-120, 120-140, or 140-160 mg of two or more metabolic inhibitors. Preferably, the kit of parts contains at least 1, 5, 10, 20, 40, 60, 80, 100, 120, 140, or 160 mg of 2-DG. Although not specifically enumerated, all values and subranges within the above ranges are specifically included as if explicitly written out.

Preferably, the kit of parts contains additionally contains at least one, two, three, or four of the HIV inhibitors listed below. Most preferably, the kit of parts contains at least one of the combination antiretroviral therapies listed below.

HIV Inhibitors

Entry inhibitors (or fusion inhibitors) interfere with binding, fusion and entry of HIV-1 to the host cell by blocking one of several targets (Wikipedia). Maraviroc works by targeting CCR5, a co-receptor located on human helper T-cells. Enfuvirtide is a peptide drug that must be injected and acts by interacting with the N-terminal heptad repeat of gp41 of HIV to form an inactive hetero six-helix bundle, therefore preventing infection of host cells.

Nucleoside reverse transcriptase inhibitors (NRTI) and nucleotide reverse transcriptase inhibitors (NtRTI) are nucleoside and nucleotide analogues which inhibit reverse transcription. Examples of NRTIs include zidovudine, abacavir, lamivudine, emtricitabine, and tenofovir.

Non-Nucleoside reverse transcriptase inhibitors (NNRTI) inhibit reverse transcriptase by binding to an allosteric site of the enzyme. 1st generation NNRTIs include nevirapine and efavirenz. 2nd generation NNRTIs include etravirine and rilpivirine.

Integrase inhibitors (also known as integrase nuclear strand transfer inhibitors or INSTIs) inhibit the viral enzyme integrase. Integrase inhibitors include raltegravir, elvitegravir, and dolutegravir. Protease inhibitors block the viral protease enzyme necessary to produce mature virions upon budding from the host membrane. Examples of HIV protease inhibitors are lopinavir, indinavir, nelfinavir, amprenavir, ritonavir, darunavir, and atazanavir.

Maturation inhibitors have a similar effect by binding to gag, and include bevirimat and vivecon.

Combination antiretroviral therapy (cART) is a mixture of at least two, and preferably three or more different classes of antiretroviral therapy. All different combinations of the antiretroviral therapies specified herein are specifically contemplated. Examples of cART include:

Combivir: lamivudine + zidovudine.

Kaletra: lopinavir + ritonavir

Trizivir: abacavir + lamivudine + zidovudine

Epzicom (in USA)/ Kivexa (in Europe and Russia): abacavir + lamivudine.

Truvada: tenofovir disoproxil fumarate + emtricitabine.

Atripla: emtricitabine + tenofovir disoproxil fumarate + efavirenz

Complera (in USA)/ Eviplera (in Europe and Russia): emtricitabine + rilpivirine + tenofovir disoproxil fumarate.

Stribild: elvitegravir + cobicistat + emtricitabine + tenofovir disoproxil fumarate.

Triumeq: abacavir + dolutegravir + lamivudine.

Evotaz: atazanavir + cobicistat.

Prezcobix: darunavir + cobicistat.

Dutrebis: lamivudine + raltegravir.

Genvoya: elvitegravir + cobicistat + emtricitabine + tenofovir alafenamide fumarate.

Descovy: emtricitabine + tenofovir alafenamide fumarate.

Rev inhibitors interfere with the biogenesis of viral RNA required for the replication of HIV. Rev inhibitor can function through binding to the Cap Binding Complex at the 5' end of the mRNA coding for 3 structural proteins of the virus. By promoting HIV RNA splicing, these inhibitors can reduce the level of genomic RNA and inhibit HIV replication. Preferred compounds can be found in U.S. Patents 9,145,367 and 9,061,999, which are hereby incorporated by reference. Particularly preferred compounds are 10-chloro-2,6- dimethyl-2H-pyrido [3',4':4,5]pyrrolo[2,3-g]isoquinoline (IDC16), 8-chloro-N-(4- (trifluoromethoxy)phenyl)quinolin-2-amine (ABX464) and 8-chloro-N-glucuronide-N-(4- (trifluoromethoxy)phenyl)quinolin-2-amine) (ABX464-N-glucuronide) compounds, as set forth in Campos et al. Retrovirology (2015) 12:30, which is hereby incorporated by reference.

A particularly preferred compound has the formula:

EXAMPLES

EXAMPLE 1. Isolation and culture of CD4+ T-cells

CD4+ T-cells were purified (>90%) from freshly isolated PBMCs by negative selection with antibody-coated magnetic beads (EasySep™ Human CD4+ T-cell Enrichment Kit Ref.19052) in a Robosep instrument (Stem Cell Technology).

Purified CD4+ cells (106 cell/mL) were cultured in RPMI 1640 containing GlutaMAX, 10% FCS, penicillin (10 lU/mL) and streptomycin (10 pg/mL) in the presence of IL-2 (Miltenyi) at 50 lU/mL (Culture media). Depending on the experiment, cells were left unstimulated or were stimulated for 3 or 5 days with 0.5 pg/mL soluble antiCD3 (BioLegend, Ref.300414, Clone UCHT1) in the absence of CD28 co-stimulation as previously described (Saez-Cirion et al., 2011). Different compounds that target metabolic pathways [2-deoxy-glucose, 2-DG (Seahorse Biotechnologies); (+)-Etomoxir sodium salt hydrate (Sigma, Ref. E1905); UK5099 (Sigma, Ref. PZ0160); 6-diazo-5-oxo-l-norleucine (DON)(Sigma, Ref. D2141); glucose (Seahorse Biotechnologies); oligomycin (Seahorse Biotechnologies) or carbonyl cyanide 4- (trifluoromethoxy)phenylhydrazone FCCP (Seahorse Biotechnologies)] were added to the culture media at different times and concentrations depending on the protocol conditions. A glucose-free culture media was used in some infection experiments and is described in the results section [RPMI non-glucose, GlutaMAX, containing 10% FCS, penicillin (10 lU/mL) and streptomycin (10 pg/mL) in the presence of IL-2 (Miltenyi) at 50 lU/mL (culture media)]. After culture, living cells were counted with an automatic Countess cell counter (Invitrogen) based on size and non-staining with trypan blue. The number of living cells was then normalized before analysis.

EXAMPLE 2. HIV infection in vitro

Single-round infections were performed with HIV-1 NL4.3AenvAnef/GFP (Amara et al., 2003) and HIV-l-DuoFluoAenv(R7GEmC) (provided by Professor Eric Verdin and Dr. Calvanese, NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: Cat# 12595 DuoFluo (R7GEmC)) (Calvanese et al., 2013). Both viruses were pseudotyped with the VSV-G envelope protein by transiently cotransfecting (SuperFect; Qiagen) 293T cells with the proviral vectors and the VSV-G expression vector pMD2.G. Nonactivated or activated CD4 + T-cells were infected in triplicate (5xl04 cells/well, 200 m|_) with 35 ng/lxlO6 HIV-1 NL4.3Anef/GFP/VSV-G and with 70 ng of HIV- l-DuoFluo(R7GEmC)/VSVg per million cells. Active HIV-1 infection was estimated by flow cytometry (BD LSRII, BD bioscience) as the percentage of GFP-expressing CD4+ T-cells 72 h after infection. Latent HIV infection was estimated by flow cytometry as the percentage of mCherry+GFP-CD4+ T-cells 72 h after infection with HIV-l-DuoFluo(R7GEmC) particles.

HIV-1 reverse transcripts (U5-Gag) were quantified by real-time PCR with an Applied Biosystems 7500 Real-Time PCR System 6, 16 and 72 h after infection of CD4+ T-cells with VSV- G-pseudotyped HIV-1 particles as described in (David et al., 2006). Briefly, total DNA was extracted with the NucleoSpin 8/96 Tissue Core kit (Macherey-Nagel, Ref. 740453.4) and 100 ng of template DNA were used per reaction. DNA loading was controlled by concurrently amplifying the albumin gene by real-time PCR and quantifying with reference to a control human genomic DNA (Roche). The reaction mixture contained lx TaqMan Universal PCR master mix, 300 nM of primers and 200 nM of the fluorogenic probe, in a final volume of 30 pi. PCR cycle conditions were: 50°C for 2 min, 95°C for 10 min, and 40 cycles of 95°C for 15 s and 60°C for 1 min. Copy numbers of U5-Gag were determined with reference to a standard curve prepared by concurrent amplification of serial dilutions of 8E5 cells containing one integrated copy of HIV-1 per cell.

Productive HIV-1 infection in vitro was studied in suboptimally activated CD4+ T-cells (106 cells/mL in triplicate) exposed to the HIV-1 BaL strain (R5) (10 ng p24/ml). The cells were cultured in 96-U-well plates for 14 days in the presence or absence of 2-DG (5 mM). Every 3-4 days, the culture supernatants were removed and replaced with fresh culture medium with or without 2-DG. Viral replication was monitored in the supernatants by p24 enzyme-linked immunosorbent assay (ELISA) (XpressBio) or at day 3 by intracellular p24 staining (p24-FITC (clone KC57, Coulter) (Saez-Cirion et al., 2010).

EXAMPLE 3. Flow-assisted sorting of CD4 + T-cell subsets

Cells were first selected based on size and structure to eliminate cellular debris. Then cell singlets and living cells (not stained with LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, Thermofisher) are gated before proceeding with further selection based on phenotypical or functional markers (Figures 9, 12, 13). Resting (CD25-, CD69-, HLA-DR-) CD4+ T-cell subsets [naive (Tn; CD3+, CD4+, CD45RA+, CCR7+, CD27+, CD95-), central memory (Tcm; CD3+, CD4+, CD45RA-, CCR7+, CD27+), transitional memory (Ttm; CD3+, CD4+, CD45RA-, CCR7-, CD27+) or effector memory (Tem; CD3+, CD4+, CD45RA-, CCR7-, CD27-)] were sorted on a FACS ARIA III cell sorter (BD) using the following antibody panel: CD3-eFLuor450 (eBioscience), CD4-alexaFluor700 (BD), CD45RA-ECD (BC), CCR7-PE_Cy7 (BioLegend), CD27-APC (Miltenyi), CD95-PE (Miltenyi), CD25-FITC (BD), CD69- FITC (eBioscience) and HLA-DR-FITC (BD). The gating strategy is depicted in Figure 9. The number of sorted cells varied from 0.5 to 5 million cells depending on the CD4+ T-cell subset and the donor. The purity of the sorted subset was greater than 98%.

GFP+ and GFP- CD4+ T-cells were sorted 72 h after infection with VSV-G pseudotyped NL4.3AenvAnef/GFP particles (Figure 12A). For some experiments, GFP+ and GFP- cells were also sorted into the following categories based on their expression of activation markers (CD25- ECD, HLA-DR_PerCyP5.5) (Figure 4 and Figure 12B): high activation GFP+ [H/+ (GFP+, CD25+,HLA-DR+)]; high activation GFP- [H/- (GFP-, CD25+, HLA-DR+)]; low activation GFP+ [L/+, (GFP+, CD25-,HLA-DR-)]; and low activation GFP- [L/- (GFP-, CD25-,HLA-DR-)].

For some experiments, CD4+ Tn and Tcm cells were sorted based on their level of glucose uptake after 5 days of stimulation with anti-CD3 (Figure 13A). The cells were washed and incubated with 2-NBDG (2-(N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl)amino)-2-deoxyglucose) (Thermo Fisher, Ref. N13195) at 75 mM in PBS for 30 min at 37°C. After 3 washes of 10 min each with fresh PBS, the cells were stained with antibodies (CD3-eFLuor450 (eBioscience), CD4- alexaFluor700 (BD), CD45RA-ECD (BC), CCR7-PE_Cy7 (BioLegend), and CD27-APC (Miltenyi)) and sorted as follows: Tn HGIu (CD3+, CD4+, CD45RA+, CCR7+, CD27+, 2NBDG+); Tn LGIu (CD3+, CD4+, CD45RA+, CCR7+, CD27+, 2NBDG-); Tcm HGIu (CD3+, CD4+, CD45RA-, CCR7+, CD27+, 2NBDG+); and Tcm LGIu (CD3+, CD4+, CD45RA-, CCR7+, CD27+, 2NBDG-).

EXAMPLE 4. Surface GLUT1 staining

CD4+ T-cells were stained with HRBD-rFc, a recombinant fusion protein that specifically binds GLUT1 (Metafora-biosystems, Paris, France), and a secondary goat-anti-Mouse Alexa Fluor 647 antibody (Thermofisher). HRBD is derived from the receptor-binding domain of the human T-cell leukemia virus envelope glycoprotein that binds the extracellular domain of GLUT1 (Manel et al., 2005). The following antibody panel was used to determine CD4+T cell subsets: CD3- eFLuor450 (eBioscience), CD4-alexaFluor700 (BD), CD45RA-ECD (BC), CCR7-PE_Cy7 (BioLegend), CD27-PE (BD bioscience).

EXAMPLE 5. Quantitative RT-PCR arrays

The expression levels of an array of 96 genes in the CD4+ T-cell subsets were quantified by RT-qPCR with a Biomark HQ system. Total RNA was extracted from 5xl04 CD4+ T-cells with an RNA trace kit (Macherey-Nagel, Ref. 740731.4) and treated with DNase, following the manufacturer's instructions. Twenty microliters of RNA (> 10 ng) was reverse transcribed with Reverse Transcription Master Mix (Fluidigm, 100-6298) (5 minutes at 25°C, 30 minutes at 42°C, and 5 minutes at 85°C). A specific target preamplification (STA) was performed by adding PreAmp Master Mix, 96 Primers Mix and EDTA to the cDNA, followed by STA cycling (95°C: 2 min, 18 cycles of [96°C: 5 s, 60°C 4 min]). The sample was then treated with exonuclease I (New England Biolabs) (37°C: 30 min, 80°C: 15 min). Sample premix (SsoFast EvaGreen Supermix with Low ROX (Biorad), DNA Binding Dye (Fluidigm), preamplified Exo 1-treated sample) and assay mix (assay loading reagent (Fluidigm), Delta Gene primers (Fluidigm)) were then loaded on primed 96.96 Dynamic Array chips (Fluidigm). The chips were transferred into a Biomark HQ device (Fluidigm) for thermocycling, and fluorescence was acquired with the GE 96x96 PCR+Melt v2 program. Linear derivative mode baseline correction was applied. We used the Normfinder algorithm (Aarhus University Hospital, Denmark) (Andersen et al., 2004) to identify the optimal normalization gene among the assayed candidates for our experimental conditions. BENC1 was thus identified as the optimal normalization gene based on expression stability in the analyzed samples (Table 5), and the gene expression values were plotted as 2-DDa, where AACt= ACtsAMPLE- ACtcoNTROb arid ACt = QTARGET CENE - CtBENCi.

EXAMPLE 6. Measurement of oxygen consumption and extracellular acidification rates

The oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured using a Seahorse XF96 metabolic analyzer following the procedure recommended by the manufacturer. Briefly, for all the experiments, different CD4+ T-cell populations were seeded at a concentration of 2xl05 cells per well on XF96 plates (Seahorse Bioscience) precoated with 0.5 mg/ml Cell Tack (Corning, Ref. 354240) immediately before adding Seahorse XF culture media to each well. Cells were incubated for 50 min in a C02-free incubator at 37°C before loading the plate in the Seahorse analyzer. Different programs were run on the Seahorse analyzer depending on the assay. Drug Panel A (1) XFmedia 2) oligomycin (2.5 pM), 3) FCCP (0.9 pM) and 4) rotenone (1 pM) and antimycin A (1 pM)) was injected through ports A, B, C and D, respectively, for the mitochondrial stress test. Drug Panel B ( 1) XFmedia 2) glucose (10 mM) 3) oligomycin (2.65 pM), and 4) 2-DG (100 mM)) was used for the glycolysis stress test.

EXAMPLE 7. Phenotyping after sorting

In some experiments, sorted GFP+/- CD4+ T-cells subset or CD4+ T bulk cells previously infected with NL4.3Anef/GFP/VSV-G with or without 2-DG or Etomoxir were incubated with CD3-eFLuor450 (eBioscience), CD4-alexaFluor700 (BD Biosciences), CD45RA-ECD (BC), CCR7- PE_Cy7 (BioLegend) and CD27-APC (Miltenyi) to determine the CD4+ T-cell subset distribution. In addition, the activation levels of sorted CD4+ T-cell subsets were assessed with CD25-ECD (BD Biosciences) and HLA-DR-FITC (BD Biosciences). For both protocols, cells were incubated with the antibodies for 25 minutes and then washed in PBS plus 1% FCS and fixed in 4% paraformaldehyde for flow cytometry on an LSRII device (BD Biosciences). The data were analyzed with Kaluza software (Beckman Coulter).

EXAMPLE 8. HIV-1 reactivation in CD4+ T-cells from HIV-l-infected individuals.

Freshly isolated CD4+T-cells (negative selection kit, Stem Cell) from HIV-individuals undergoing successful cART were seeded in 48-well plates (lxlO6 cells/well, in triplicate) and stimulated with phytohemagglutinin-L (PHA-L, Roche, 1 pg/mL) and IL-2 (Miltenyi) 100UI with or without 2-DG (5 mM). The culture supernatants were collected every 3 to 4 days, and fresh medium +/- 2-DG was added to the cultures. Supernatants were stored at -80°C, and HIV-1 p24 was analyzed later by ultrasensitive digital ELISA (Simoa, Quanterix) (Passaes et al., 2017).

EXAMPLE 9. Differential gene expression

For each gene, we implemented a mixed effects model to detect differential expression between cell types (Tn, Tcm, Ttm and Tern). We defined a model that included the type of cells as a fixed effect and the patient as a random effect. A p-value was then obtained by implementing a likelihood ratio test between the full model and a reduced model without the fixed effect. Heat maps were generated by K-means clustering. Data were filtered by variance (6/6max=0.2) to reduce background noise. Gene expression data were centered to a mean value of zero and scaled to unit variance.

EXAMPLE 10. Correlation between gene expression, metabolic parameters and HIV-1 susceptibility

We computed Spearman's correlation coefficient and tested for significance.

Values are presented in the graphs as medians and interquartile ranges. Statistical analyses were performed using SigmaPlot (Systat Software). The asterisks represent statistically significant differences (*p<0.05; ** p<0.01). Differences between CD4+ T-cell subsets in different conditions were analyzed with nonparametric signed ANOVA and the multiple comparison Student-Newman-Keuls method. Differences between GFP+ and GFP- CD4+ T-cells or control vs treatment culture conditions were analyzed with paired t-tests. When multiple treatment conditions were tested, ANOVA analyses and the Holm-Sidak method for multiple comparisons versus control group were used, and significant differences between experimental conditions were shown as horizontal lines.

EXAMPLE 11. CD4+ T-cell subsets have heterogeneous susceptibility to HIV-1 infection

We first assessed the relative intrinsic susceptibility of primary CD4+ T-cell subsets (naive, Tn; central memory, Tcm; transitional memory, Ttm; and effector memory, Tern) to HIV- 1 infection. We used single-cycle NL4.3AenvGFP particles pseudotyped with VSV-G envelope protein (HIV-1GFP-VSV) to circumvent differences in the surface expression of CCR5 across CD4+ T-cell subsets. We activated CD4+ T-cells with soluble anti-CD3. This 'suboptimal' activation protocol has allowed us to expose differences in the susceptibility to HIV-1 of CD4+ T-cells from different individuals that were masked using more potent stimulation protocols (Saez-Cirion et al., 2011). Activation enhanced the susceptibility to HIV-1 without altering the relative contribution of each CD4+ T-cell subset (Figure 1A, B). After infection, the relative frequencies of Tn, Tcm, Ttm and Tern cells among GFP-negative (GFP-) cells was identical to that among noninfected CD4+ T-cells (Figure IB). In contrast, the composition of HIV-infected GFP-positive (GFP+) cells was different from that of noninfected cells, with a significant exclusion of Tn cells and strong enrichment of Tern cells. Tcm cells were also slightly underrepresented, and Ttm cells were overrepresented among GFP+ CD4+ T-cells when compared to the control condition (Figure IB). These results suggested different susceptibilities to HIV-1 infection of CD4+ T-cell subsets, with Tem cells being the most susceptible, followed by Ttm and Tcm cells, and with Tn cells being strongly resistant to infection.

To study if these differences were related to the inherent program of each CD4+ T-cell subset, we isolated quiescent CD4+ Tn, Tcm, Ttm and Tem cells (n=6 donors, Figure 9), and we analyzed their susceptibility to HIV-1 with or without activation. Activation enhanced the susceptibility of all CD4+ T-cell subsets to HIV-1 infection (Figure 1C). However, this effect was variable according to the subset. There was a tendency for Tem cells to be more susceptible than other subsets (p=0.06) in the absence of activation, and this difference became more pronounced after three (p=0.0004, all comparisons) or five days of activation (p=0.012, Tem vs Tn and Ttm and Tcm vs Tn and Ttm). Overall, our results recapitulated previous observations showing an inherent hierarchy in the susceptibility of CD4+ T-cell subsets to HIV-1 infection (Buzon et al., 2014; Tabler et al., 2014).

EXAMPLE 12. Levels of HIV infection are related to the molecular program of CD4+ T-cell subsets

To determine if a molecular program was associated with the susceptibility of CD4+ T- cell subsets to HIV infection, we analyzed the expression of a panel of 96 genes (related to T-cell activation, survival, differentiation and function as well as known viral restriction or HIV facilitating factors, Table 3) in each CD4+ T-cell subpopulation at the time of infection.

Nonactivated CD4+ T-cell subsets showed distinct transcriptional profiles that were further enhanced after activation (e.g., 34 genes and 49 genes differently expressed between CD4+ T- cell subsets without activation and after 3 days of anti-CD3 treatment, respectively, Figure 2A). These genes were mostly related to signal transduction and the response to stimulus, which could be related to the previously described different susceptibility to CD3 activation of the CD4+ T-cell subsets (Croft et al., 1994; Kumar et al., 2011). The level of HIV-infected cells correlated with the expression of several genes at the time of infection in the different conditions studied (Figure 2B and Figure 10). SAMHD1 showed a negative association with infection. In contrast, positive correlations were observed between infection levels and other antiviral factors (such as APOBEC3G or SLFN ll)(Li et al., 2012; Sheehy et al., 2002) as well as several genes involved in the interferon response (IFI6, IFI16, EIF2AK2, and OAS1) (Kane et al., 2016). Significant positive correlations were also observed between the level of HIV infection and the gene expression levels of transcription factors (STAT3, E2F1, and PRDM1), genes that have been proposed to facilitate HIV-1 infection (RRM2, HSP90AA, CFL1, and DYNClHl)(Allouch et al., 2013; Franke and Luban, 1996; Lukic et al., 2014; Roesch et al., 2012) and multiple genes involved in T-cell metabolism (SLC2A3, SLC2A1, SLC2A5, CASP3, FAS, GAPDH, and GUSB). Taken together, these results suggest that, with the exception of SAMHD1, the antiviral restriction factors analyzed did not decisively influence the cell susceptibility to HIV-1, which is in line with the results of previous reports (Jia et al., 2015). Our data indicate that metabolically active cells may offer favorable conditions for HIV infection.

EXAMPLE 13. Hierarchy of susceptibility to HIV infection matches metabolic activity of CD4+ T- cell subsets

To explore the possible association between HIV infection and cell metabolism, we determined the metabolic activity of the CD4+ T-cell subsets at the time of infection. We used a cell flux analyzer to measure, in different conditions, the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) as indicators of oxidative phosphorylation (OXPHOS) and glycolysis, respectively (Zhang et al., 2012). In the absence of activation in vitro and in agreement with their quiescent nature, all sorted CD4+ T-cell subsets had low levels of metabolic activity (Figure 3A). Nonetheless, small differences between subsets were noted; basal metabolism and metabolic potential were highest in Tern cells and lowest in Tn cells, while Ttm and Tcm cells presented similar intermediate levels (Figures 3A, B). These differences were more pronounced after activation, with all memory cell subsets increasing mitochondrial function and glycolysis to different extents and with different kinetics. The highest metabolic activity was measured in Tern cells, peaking on day 3 after activation and decreasing on day 5. The metabolism of Ttm and Tcm cells increased after 3 days of activation and then remained stable in Ttm cells while continuing to increase in Tcm cells. In contrast, Tn cells showed a modest increase only in mitochondrial function and not in glycolysis and only after 5 days of activation, when their metabolism was heavily relying on OXPHOS (Figure 3C). Accordingly, important differences were also found between CD4+ T-cell subsets regarding their capacity to uptake glucose and their levels of the surface expression of the GLUT1 receptor, which were lowest in Tn cells and highest in Tem cells (Figures 11A, B). The relative metabolic activity levels of the different cell subsets matched their relative susceptibility to HIV-1 infection (Figure 1C), and we found positive correlations between HIV infection levels and multiple metabolic functions in cells that had been activated (Figure 3D, Figure 11C). These results further point to an influence of the metabolic activity of CD4+ T-cells on their susceptibility to HIV-1.

EXAMPLE 14. HIV-infected CD4+ T-cells are characterized by higher levels of metabolic activity independent of cell activation levels

To analyze if there was a direct link between cell metabolism and HIV-1 infection, we challenged 5-day activated bulk CD4+ T-cells with HIV-lgfP-VSV, and we sorted three days later noninfected GFP- and infected GFP+ cells. HIV-infected CD4+ T-cells had higher levels of basal metabolism and metabolic potential and, overall, a more energetic profile than noninfected cells (Figures 4A and S4A). Although we could detect infected cells among cells with low activation levels (Figure 4B), we found higher proportions of GFP+ cells among CD4+ T-cells expressing activation markers. We therefore evaluated whether differences in the metabolic activity of infected and noninfected CD4+ T-cells were just a consequence of the selective infection of CD4+ T-cells with higher activation levels. We sorted CD4+ T-cells first based on their expression of either high or low levels of both HLA-DR and CD25 and then based on whether they were GFP+ or GFP- (Figures 4B and S4). After 5 days of stimulation, the CD4+ T-cell subsets expressed different levels of activation markers (Figure 12B), which were highest in Tem cells and lowest in Tn cells. This was translated to different contributions of CD4+ T-cell subpopulations in the high- and low-activation sorted cell fractions (Figure 4B). Nevertheless, Tn cells were more frequently found in the GFP- fraction, both in high and low-activated cell populations, whereas the GFP+ fraction was enriched with Tem cells. These results matched the hierarchy of infection that we observed before (Figure 1) and further supported that the susceptibility of CD4+ T-cell subsets to HIV-1 depends on the intrinsic characteristics of these cells independent of their activation status. In this regard, we found that infected GFP+ cells in both the high- and low-activation fractions, had higher basal metabolisms (OCR and ECAR) than noninfected GFP- cells (Figure 4C). These results demonstrated that HIV-infected CD4+ T-cells were characterized by higher metabolic activity levels.

EXAMPLE 15. HIV-1 infection is preferentially established in CD4+ T-cells with high metabolic activity levels

Our results suggest that HIV-1 infection is favored in the environment provided by CD4+ T-cells with high metabolic activity levels. We analyzed if this was due to a selective infection of CD4+ T-cells with the highest metabolic activity levels or if it was HIV-infection that increased the metabolic activity of the cells. We activated CD4+ T-cells and sorted Tn and Tcm cells based on their capacity to uptake high or low levels of the fluorescent glucose analogue 2NBDG (Figure 13A), which corresponded to weakly and strongly glycolytic cells respectively (Figure 13B). We infected these purified cell fractions with HIV-lgfp-VSV. Three days after infection, infected GFP+ CD4+ T-cells were only observed among highly glycolytic Tn and Tcm cells, while weakly glycolytic cells were strongly resistant to infection (65x [41x-206x], median [IQR] fold increase in the proportion of GFP+ cells in HGIu vs LGIu cell subsets, p=0.008) (Figure 5). Overall these results confirmed that, in our conditions, the high metabolic activity of infected CD4+ T-cells was one of the causes rather than a consequence of HIV infection.

EXAMPLE 16. Suboptimal inhibition of glucose metabolism blocks HIV-1 replication in CD4+ T- cells

The above results indicate that HIV-1 infection of CD4+ T-cells required high levels of metabolic activity. Therefore, we analyzed if HIV-1 replication could be blocked with metabolic inhibitors. We infected activated CD4+ T-cells with HIV-lgfp-VSV in the presence of increasing amounts of etomoxir, an inhibitor of fatty acid oxidation (FAO), 6-diazo-5-oxo-l-norleucine (DON), a glutamine antagonist, or 2-deoxy glucose (2-DG), a competitive inhibitor of glycolysis. Etomoxir was able to reduce HIV infection but only at high concentrations, well above the levels needed to reduce mitochondrial respiration (Figure 6A and Figure 14). DON reduced HIV infection without inducing cell death, although the extent of the inhibition was heterogeneous. Suboptimal amounts of 2-DG (5 mM), which were enough to significantly reduce glycolysis (Figure 14), decreased HIV-1 infection of CD4+ T-cells with minimal cell toxicity (Figure 6A).

These results suggested a higher impact of glucose and glutamine metabolism than FAO on HIV- 1 replication. The role of glucose metabolism was further confirmed in different sets of experiments in which the frequency of HIV-l-infected CD4+ T-cells was reduced when the infections were performed in conditions of glucose starvation or in presence of UK5099, a molecule that inhibits the transport of pyruvate, an end product of glycolysis, to the

mitochondria (Figure 6B). The presence of 2-DG impaired the accumulation of HIV-1 reverse transcribed products overtime pointing to an early block of viral replication (Figure 6C). 2-DG was able to reduce infection and reverse transcript levels to a similar extent whether it was added to the culture at the time of the challenge or up to 8h later (Figure 6D), indicating that 2- DG was affecting post-entry steps of viral replication. Overall, these results show that a glycolytic environment was necessary for HIV-1 to complete reverse transcription.

2-DG blocked HIV-1 infection in all CD4+ T-cell subsets, although the differences were more pronounced in more differentiated (more glycolytic) cells (Figure 6E). Interestingly, Etomoxir slightly reduced viral replication in Tern cells but not in other T-cell subsets, which could be related to the overall highly energetic nature of these cells. We then used VSV-G pseudotyped NL4.3Aenv Duo-Fluo I particles that allow HIV-1 latently and productively infected cells to be distinguished from each other (Calvanese et al., 2013) (Figure 15A). Interestingly, latent infection was more prominent among Tn and Tcm CD4+ T-cells, while productive infection was predominantly observed among Tern cells (Figure 15A). Overall, the presence of 2- DG significantly reduced the global number of both latently and productively infected CD4+ T- cells (Figure 6F), which agreed with the need for a glycolytic environment for HIV-1 to complete the preintegration steps of its replication cycle.

We then analyzed the impact of inhibition of glycolysis on the infection of CD4+ T-cells with a R5 wild-type replication competent virus (HIV-1 Bal). We first confirmed that the hierarchy of infection of CD4+ T-cell subsets that we observed with VSV-G single cycle particles (Tn<Tcm<Ttm<Tem) coincided with the hierarchy of infection when we used replication competent HIV-1 Bal (Figure 15B). We found that 2-DG was also able to efficiently blocked infection of CD4+ T-cells with HIV-1 Bal (Figure 6G), independently of whether it was added at the time of challenge or 4h/8h after challenge (Figure 15C). All together, these results show that the inhibition of metabolic activity blocked HIV-1 replication, corroborating that CD4+ T-cell metabolism is an important determinant of HIV-1 infection.

EXAMPLE 17. Suboptimal inhibition of glycolysis eliminates HIV-1 infected cells and impairs HIV amplification from CD4+ T-cell reservoirs

We next studied if the preferential establishment of HIV-1 infection in highly glycolytic cells could be used to target HIV-1 reservoirs. First, we analyzed if suboptimal inhibition of glycolysis could selectively eliminate CD4+ T-cells that had been preinfected in vitro. We infected CD4+ T-cells with HIV-lgfP-VSV and sorted infected GFP+ from noninfected GFP- cells (Figure 12A) and cultured them in the absence or presence of 2-DG to inhibit glycolysis. The presence of 2-DG induced higher levels of cell death among infected GFP+ cells than among GFP- cells (Figure 7A and B), affecting all memory T-cell subpopulations (Figure 7C).

As 2-DG was able to both block infection and eliminate infected cells, we wondered whether 2-DG could block HIV spread upon activation of CD4+ T-cells from HIV-infected individuals receiving cART. We isolated CD4+ T-cells from 6 individuals receiving cART (Table 4) and activated the cells with PHA in the absence or presence of 2-DG. In all cases, 2-DG potently blocked HIV-1 amplification, as measured by ultrasensitive analyses of p24 production (Figure 7D). Therefore, the need of HIV for highly glycolytic cells reveals a vulnerability that can be exploited to tackle infection.

EXAMPLE 18. Synergism of multiple metabolic inhibitors

CD4+ T cells from a same donor were treated with various metabolic inhibitors, alone or in combination. The results are shown in Figure 8, which shows the synergistic effect of suboptimal amounts of 2DG, DON and ETOMOXIR.

REFERENCES

Allouch, A., David, A., Amie, S.M., Lahouassa, H., Chartier, L, Margottin-Goguet, F., Barre- Sinoussi, F., Kim, B., Saez-Cirion, A., and Pancino, G. (2013). p21-mediated RNR2 repression restricts HIV-1 replication in macrophages by inhibiting dNTP biosynthesis pathway. Proc Natl Acad Sci U S A 110, E3997-4006.

Amara, A., Vidy, A., Boulla, G., Mollier, K., Garcia-Perez, J., Alcami, J., Blanpain, C., Parmentier, M., Virelizier, J.L., Charneau, P., et al. (2003). G protein-dependent CCR5 signaling is not required for efficient infection of primary T lymphocytes and macrophages by R5 human immunodeficiency virus type 1 isolates. J Virol 77, 2550-2558.

Amie, S.M., Noble, E., and Kim, B. (2013). Intracellular nucleotide levels and the control of retroviral infections. Virology 436, 247-254.

Andersen, C.L., Jensen, J.L., and Orntoft, T.F. (2004). Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64, 5245-5250.

Banga, R., Procopio, F.A., Noto, A., Pollakis, G., Cavassini, M., Ohmiti, K., Corpataux, J.M., de Leval, L., Pantaleo, G., and Perreau, M. (2016). PD-1(+) and follicular helper T cells are responsible for persistent HIV-1 transcription in treated aviremic individuals. Nature medicine 22, 754-761.

Barton, K., Winckelmann, A., and Palmer, S. (2016). HIV-1 Reservoirs During Suppressive Therapy. Trends in microbiology 24, 345-355.

Besnard, E., Hakre, S., Kampmann, M., Lim, H.W., Hosmane, N.N., Martin, A., Bassik, M.C., Verschueren, E., Battivelli, E., Chan, J., et al. (2016). The mTOR Complex Controls HIV Latency. Cell Host & Microbe 20, 785-797.

Burke, J.D., Platanias, L.C., and Fish, E.N. (2014). Beta Interferon Regulation of Glucose Metabolism Is PI3K/Akt Dependent and Important for Antiviral Activity against Coxsackievirus B3. J Virol 88, 3485-3495.

Buzon, M.J., Sun, H., Li, C., Shaw, A., Seiss, K., Ouyang, Z., Martin-Gayo, E., Leng, J., Henrich, T.J., Li, J.Z., et al (2014). HIV-1 persistence in CD4+ T cells with stem cell-like properties. Nature medicine 20, 139-142.

Byrne, J.A., Butler, J.L., and Cooper, M.D. (1988). Differential activation requirements for virgin and memory T cells. J Immunol 141, 3249-3257.

Calvanese, V., Chavez, L., Laurent, T., Ding, S., and Verdin, E. (2013). Dual-color HIV reporters trace a population of latently infected cells and enable their purification. Virology 446, 283-292.

Chan, 0., Burke, J.D., Gao, D.F., and Fish, E.N. (2012). The Chemokine CCL5 Regulates Glucose Uptake and AMP Kinase Signaling in Activated T Cells to Facilitate Chemotaxis. J Biol Chem 287, 29406-29416.

Chomont, N., El-Far, M., Ancuta, P., Trautmann, L., Procopio, F.A., Yassine-Diab, B., Boucher, G., Boulassel, M.R., Ghattas, G., Brenchley, J.M., et al. (2009). HIV reservoir size and persistence are driven by T cell survival and homeostatic proliferation. Nature medicine 15, 893-900.

Cleret-Buhot, A., Zhang, Y., Planas, D., Goulet, J.-P., Monteiro, P., Gosselin, A., Wacleche, V.S., Tremblay, C.L., Jenabian, M.-A., Routy, J.-P., et al. (2015). Identification of novel HIV-1 dependency factors in primary CCR4+CCR6+Thl7 cells via a genome-wide transcriptional approach. Retrovirology 12, 102.

Croft, M., Bradley, L.M., and Swain, S.L. (1994). Naive versus memory CD4 T cell response to antigen. Memory cells are less dependent on accessory cell costimulation and can respond to many antigen-presenting cell types including resting B cells. J Immunol 152, 2675-2685. David, A., Saez-Cirion, A., Versmisse, P., Malbec, 0., Iannascoli, B., Herschke, F., Lucas, M., Barre-Sinoussi, F., Mouscadet, J.F., Daeron, M., et al. (2006). The engagement of activating FcgammaRs inhibits primate lentivirus replication in human macrophages. J Immunol 177, 6291-6300.

Deeks, S.G., Lewin, S.R., Ross, A.L., Ananworanich, J., Benkirane, M., Cannon, P., Chomont, N., Douek, D., Lifson, J.D., Lo, Y.R., et al. (2016). International AIDS Society global scientific strategy: towards an HIV cure 2016. Nature medicine 22, 839-850.

Descours, B., Cribier, A., Chable-Bessia, C., Ayinde, D., Rice, G., Crow, Y., Yatim, A., Schwartz, 0., Laguette, N., and Benkirane, M. (2012). SAMHD1 restricts HIV-1 reverse transcription in quiescent CD4(+) T-cells. Retrovirology 9, 87.

Finzi, D., Hermankova, M., Pierson, T., Carruth, L.M., Buck, C., Chaisson, R.E., Quinn, T.C., Chadwick, K., Margolick, J., Brookmeyer, R., et al. (1997). Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy. Science 278, 1295-1300.

Franke, E.K., and Luban, J. (1996). Inhibition of HIV-1 replication by cyclosporine A or related compounds correlates with the ability to disrupt the Gag-cyclophilin A interaction. Virology 222, 279-282.

Fritsch, S.D., and Weichhart, T. (2016). Effects of Interferons and Viruses on Metabolism. Front Immunol 7.

Fromentin, R., Bakeman, W., Lawani, M.B., Khoury, G., Hartogensis, W., DaFonseca, S., Killian, M., Epling, L., Hoh, R., Sinclair, E., et al. (2016). CD4+ T Cells Expressing PD-1, TIGIT and LAG-3 Contribute to HIV Persistence during ART. PLoS Pathog 12, el005761.

Goodwin, C.M., Xu, S., and Munger, J. (2015). Stealing the Keys to the Kitchen: Viral Manipulation of the Host Cell Metabolic Network. Trends in microbiology 23, 789-798. Hegedus, A., Kavanagh Williamson, M., and Huthoff, H. (2014). HIV-1 pathogenicity and virion production are dependent on the metabolic phenotype of activated CD4+ T cells. Retrovirology 11, 98.

Jia, X., Zhao, Q., and Xiong, Y. (2015). HIV suppression by host restriction factors and viral immune evasion. Current opinion in structural biology 31, 106-114.

Jones, W., and Bianchi, K. (2015). Aerobic Glycolysis: Beyond Proliferation. Front Immunol

6

Kane, M., Zang, T.M., Rihn, S.J., Zhang, F., Kueck, T., Alim, M., Schoggins, J., Rice, C.M., Wilson,

S.J., and Bieniasz, P.D. (2016). Identification of Interferon-Stimulated Genes with Antiretroviral Activity. Cell Host Microbe 20, 392-405.

Kavanagh Williamson, M., Coombes, N., Juszczak, F., Athanasopoulos, M., Khan, M.B., Eykyn,

T.R., Srenathan, U., Taams, L.S., Dias Zeidler, J., Da Poian, A.T., et al. (2018). Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1. Viruses 10.

Kulkarni, M.M., Ratcliff, A.N., Bhat, M., Alwarawrah, Y., Hughes, P., Arcos, J., Loiselle, D., Torrelles, J.B., Funderburg, N.T., Haystead, T.A., et al. (2017). Cellular fatty acid synthase is required for late stages of HIV-1 replication. Retrovirology 14, 45. Kumar, R., Ferez, M., Swamy, M., Arechaga, I., Rejas, M.T., Valpuesta, J.M., Schamel, W.W., Alarcon, B., and van Santen, H.M. (2011). Increased sensitivity of antigen-experienced T cells through the enrichment of oligomeric T cell receptor complexes. Immunity 35, 375- 387.

Lane, A.N., and Fan, T.W.M. (2015). Regulation of mammalian nucleotide metabolism and biosynthesis. Nucleic Acids Res 43, 2466-2485.

Lever, A.M., and Jeang, K.T. (2011). Insights into cellular factors that regulate HIV-1 replication in human cells. Biochemistry 50, 920-931.

Li, M., Kao, E., Gao, X., Sandig, H., Limmer, K., Pavon-Eternod, M., Jones, T.E., Landry, S., Pan, T., Weitzman, M.D., et al. (2012). Codon-usage-based inhibition of HIV protein synthesis by human schlafen 11. Nature 491, 125-128.

Lim, S., Phillips, J.B., Silva, L.M.d., Zhou, M., Fodstad, 0., Owen, L.B., and Tan, M. (2017). Interplay between Immune Checkpoint Proteins and Cellular Metabolism. Cancer Res 77, 1245-1249.

Loisel-Meyer, S., Swainson, L., Craveiro, M., Oburoglu, L., Mongellaz, C., Costa, C., Martinez, M., Cosset, F.-L., Battini, J.-L., Herzenberg, L.A., et al. (2012). Glutl-mediated glucose transport regulates HIV infection. PNAS 109, 2549-2554.

Lukic, L., Dharan, A., Fricke, T., Diaz-Griffero, F., and Campbell, E.M. (2014). HIV-1 uncoating is facilitated by dynein and kinesin 1. J Virol 88, 13613-13625.

Man, K., Miasari, M., Shi, W., Xin, A., Henstridge, D.C., Preston, S., Pellegrini, M., Belz, G.T., Smyth, G.K., Febbraio, M.A., et al. (2013). The transcription factor IRF4 is essential for TCR affinity-mediated metabolic programming and clonal expansion of T cells. Nature Immunology 14, 1155-1165.

Manel, N., Battini, J.L., and Sitbon, M. (2005). Human T cell leukemia virus envelope binding and virus entry are mediated by distinct domains of the glucose transporter GLUT1. The Journal of biological chemistry 280, 29025-29029.

Manel, N., Kim, F.J., Kinet, S., Taylor, N., Sitbon, M., and Battini, J.L. (2003). The ubiquitous glucose transporter GLUT-1 is a receptor for HTLV. Cell 115, 449-459.

Mathews, C.K. (2015). Deoxyribonucleotide metabolism, mutagenesis and cancer. Nat Rev Cancer 15, 528-539.

Mendler, A.N., Hu, B., Prinz, P.U., Kreutz, M., Gottfried, E., and Noessner, E. (2012). Tumor lactic acidosis suppresses CTL function by inhibition of p38 and JNK/c-Jun activation. Int J Cancer 131, 633-640.

O'Connor, R.S., Guo, L., Ghassemi, S., Snyder, N.W., Worth, A.J., Weng, L., Kam, Y., Philipson, B., Trefely, S., Nunez-Cruz, S., et al. (2018). The CPTla inhibitor, etomoxir induces severe oxidative stress at commonly used concentrations. Sci Rep 8, 6289.

Oliveira, N.M., Farrell, K.B., and Eiden, M.V. (2006). In Vitro Characterization of a Koala Retrovirus. J Virol 80, 3104-3107.

Palmer, C.S., Duette, G.A., Wagner, M.C.E., Henstridge, D.C., Saleh, S., Pereira, C., Zhou, J., Simar, D., Lewin, S.R., Ostrowski, M., et al. Metabolically active CD4+ T cells expressing Glutl and 0X40 preferentially harbor HIV during in vitro infection. FEBS Lett, n/a-n/a.

Palmer, C.S., Ostrowski, M., Gouillou, M., Tsai, L., Yu, D., Zhou, J., Henstridge, D.C., Maisa, A., Hearps, A.C., Lewin, S.R., et al. (2014). Increased glucose metabolic activity is associated with Cd4+ T-cell activation and depletion during chronic Hiv infection. Aids 28, 297-309. Pan, X., Baldauf, H.M., Keppler, O.T., and Fackler, O.T. (2013). Restrictions to HIV-1 replication in resting CD4+ T lymphocytes. Cell Res 23, 876-885. Passaes, C.P., Bruel, T., Decalf, J., David, A., Angin, M., Monceaux, V., Muller-Trutwin, M., Noel, N., Bourdic, K., Lambotte, 0., et al. (2017). Ultrasensitive HIV-1 p24 Assay Detects Single Infected Cells and Differences in Reservoir Induction by Latency Reversal Agents. J Virol 91. Patsoukis, N., Bardhan, K., Chatterjee, P., Sari, D., Liu, B., Bell, L.N., Karoly, E.D., Freeman, G.J., Petkova, V, Seth, P., et al. (2015). PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation. Nature Communications 6, 6692. Pearce, E.L., Poffenberger, M.C., Chang, C.H., and Jones, R.G. (2013). Fueling immunity: insights into metabolism and lymphocyte function. Science 342, 1242454.

Roederer, M., Raju, P.A., Mitra, D.K., Herzenberg, L.A., and Herzenberg, L.A. (1997). HIV does not replicate in naive CD4 T cells stimulated with CD3/CD28. J Clin Invest 99, 1555-1564. Roesch, F., Meziane, 0., Kula, A., Nisole, S., Porrot, F., Anderson, I., Mammano, F., Fassati, A., Marcello, A., Benkirane, M., et al. (2012). Hyperthermia stimulates HIV-1 replication. PLoS Pathog 8, el002792.

Saez-Cirion, A., Hamimi, C., Bergamaschi, A., David, A., Versmisse, P., Melard, A., Boufassa, F., Barre-Sinoussi, F., Lambotte, 0., Rouzioux, C., et al. (2011). Restriction of HIV-1 replication in macrophages and CD4+ T cells from HIV controllers. Blood 118, 955-964.

Saez-Cirion, A., Shin, S.Y., Versmisse, P., Barre-Sinoussi, F., and Pancino, G. (2010). Ex vivo T cell-based HIV suppression assay to evaluate HIV-specific CD8+ T-cell responses. Nat Protoc 5, 1033-1041.

Sanchez, E.L., and Lagunoff, M. (2015). Viral activation of cellular metabolism. Virology 479- 480, 609-618.

Schnittman, S.M., Lane, H.C., Greenhouse, J., Justement, J.S., Baseler, M., and Fauci, A.S. (1990). Preferential infection of CD4+ memory T cells by human immunodeficiency virus type 1: evidence for a role in the selective T-cell functional defects observed in infected individuals. Proc Natl Acad Sci U S A 87, 6058-6062.

Schoggins, J.W., and Rice, C.M. (2011). Interferon-stimulated genes and their antiviral effector functions. Current Opinion in Virology 1, 519-525.

Seo, J.-Y., Yaneva, R., Hinson, E.R., and Cresswell, P. (2011). Human Cytomegalovirus Directly Induces the Antiviral Protein Viperin to Enhance Infectivity. Science 332, 1093- 1097.

Sheehy, A.M., Gaddis, N.C., Choi, J.D., and Malim, M.H. (2002). Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 418, 646-650. Shimode, S., Nakaoka, R., Shogen, H., and Miyazawa, T. (2013). Characterization of feline ASCT1 and ASCT2 as RD-114 virus receptor. Journal of General Virology 94, 1608-1612. Sorbara, L.R., Maldarelli, F., Chamoun, G., Schilling, B., Chokekijcahi, S., Staudt, L., Mitsuya, H., Simpson, I.A., and Zeichner, S.L. (1996). Human immunodeficiency virus type 1 infection of H9 cells induces increased glucose transporter expression. J Virol 70, 7275-7279.

Stevenson, M., Stanwick, T.L., Dempsey, M.P., and Lamonica, C.A. (1990). HIV-1 replication is controlled at the level of T cell activation and proviral integration. EMBO J 9, 1551-1560. Sugiura, A., and Rathmell, J.C. (2018). Metabolic Barriers to T Cell Function in Tumors. J Immunol 200, 400-407.

T abler, C.O., Lucera, M.B., Haqqani, A.A., McDonald, D.J., Migueles, S.A., Connors, M., and Tilton, J.C. (2014). CD4+ memory stem cells are infected by HIV-1 in a manner regulated in part by SAMHD1 expression. J Virol 88, 4976-4986. Takeuchi, Y., Vile, R.G., Simpson, G., O'Hara, B., Collins, M.K., and Weiss, R.A. (1992). Feline leukemia virus subgroup B uses the same cell surface receptor as gibbon ape leukemia virus. J Virol 66, 1219-1222.

Valle-Casuso, J.C., Allouch, A., David, A., Lenzi, G.M., Studdard, L, Barre-Sinoussi, F., Muller- Trutwin, M., Kim, B., Pancino, G., and Saez-Cirion, A. (2017). p21 Restricts HIV-1 in Monocyte-Derived Dendritic Cells through the Reduction of Deoxynucleoside Triphosphate Biosynthesis and Regulation of SAMHD1 Antiviral Activity. J Virol 91.

van der Windt, G.J., O'Sullivan, D., Everts, B., Huang, S.C., Buck, M.D., Curtis, J.D., Chang, C.H., Smith, A.M., Ai, T., Faubert, B., et al. (2013). CD8 memory T cells have a bioenergetic advantage that underlies their rapid recall ability. Proc Natl Acad Sci U S A 110, 14336- 14341.

von Laer, D., Thomsen, S., Vogt, B., Donath, M., Kruppa, J., Rein, A., Ostertag, W., and Stocking, C. (1998). Entry of Amphotropic and 10A1 Pseudotyped Murine Retroviruses Is Restricted in Hematopoietic Stem Cell Lines. J Virol 72, 1424-1430.

Waickman, A.T., and Powell, J.D. (2012). mTOR, metabolism, and the regulation of T-cell differentiation and function. Immunol Rev 249, 43-58.

Yao, C.H., Liu, G.Y., Wang, R., Moon, S.H., Gross, R.W., and Patti, G.J. (2018). Identifying off- target effects of etomoxir reveals that carnitine palmitoyltransferase I is essential for cancer cell proliferation independent of beta-oxidation. PLoS Biol 16, e2003782.

Zhang, J., Nuebel, E., Wisidagama, D.R., Setoguchi, K., Hong, J.S., Van Horn, C.M., Imam, S.S., Vergnes, L., Malone, C.S., Koehler, C.M., et al. (2012). Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells. Nat Protoc 7, 1068-1085.

Zhao, G.-N., Jiang, D.-S., and Li, H. (2015). Interferon regulatory factors: at the crossroads of immunity, metabolism, and disease. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1852, 365-378.

Zhao, Y., Butler, E.B., and Tan, M. (2013). Targeting cellular metabolism to improve cancer therapeutics. Cell Death & Disease 4, e532.

H w

Table 3, related to STAR methods and Figure 2 Gene symbols, RefSeq, full gene names and primers used in the transcriptomic q-PCR analysis s

F

M, male; F, female; NA, not available.

Table 4, related to STAR methods and Figure 2

Clinical characteristics of HIV infected individuals whose CD4+ T cells were used in the study

Table 5, related to STAR methods and Figure 7

Ranking of studied genes after analysis with the Normfinder algorithm (Aarhus

University Hospital, Denmark). BECN1 was the most stable gene present in our panel and was used to normalize our RT- qPCR data

Better stability value (Best House Keeping gene)

Worse stability value (Worse House Keeping gene)

Claims

1. A method of reducing HIV production from a T cell comprising contacting a population of cells comprising HIV-infected CD4+ T cells with 2-deoxy-glucose (2-DG) at a concentration that reduces the production of HIV from said HIV-infected CD4+ T cells.

2. The method of claim 1, wherein the cells are exposed to a concentration of 1-25 mM 2-DG.

3. The method of claim 2, wherein the cells are exposed to a concentration of 3-10 mM 2-DG.

4. The method of claim 3, wherein the cells are exposed to a concentration of 5 mM 2-DG.

5. The method of claim 1, further comprising contacting the population of cells with a metabolic inhibitor that blocks fatty acid transport to mitochondria.

6. The method of claim 5, wherein the metabolic inhibitor is Etomoxir.

7. The method of any of claims 1-6, wherein the population of cells has received cART treatment.

8. The method of any of claim 1-7, wherein the cells are contacted with the metabolic inhibitor in vitro.

9. The method of any of claim 1-7, wherein the cells are contacted with the metabolic inhibitor in vivo.

10. The method of any of claims 1-9, wherein the cells are contacted with an additional metabolic inhibitor of metabolic function in CD4+ T cells.

11. The method of claim 10, wherein the cART comprises Combivir, Kaletra, Trizivir, Epzicom, Kivexa, Truvada, Atripla, Complera, Eviplera, Stribild, Triumeq, Evotaz, Prezcobix, Dutrebis, Genvoya, or Descovy.

12. The method of claim 10, wherein the cART comprises at least 2 or 3 of any of the following compounds:

lamivudine; zidovudine; lopinavir; ritonavir; abacavir; tenofovir disoproxil fumarate; emtricitabine; efavirenz; rilpivirine; elvitegravir; cobicistat; dolutegravir; atazanavir; cobicistat; darunavir; and raltegravir.

13. A method of reducing virus or bacterial pathogen production from a cell comprising contacting a population of cells pathogen-infected cells with at least 2 metabolic inhibitors of different metabolic functions in the cells at a concentration that reduces the production of the pathogen from said pathogen-infected cells.

14. The method of claim 13, wherein one of the metabolic inhibitors blocks glycolysis.

15. The method of claim 13 or 14, wherein one of the metabolic inhibitors blocks fatty acid transport to mitochondria.

16. The method of any of claims 13-15, wherein both of the metabolic inhibitors are selected from Table 1 or Table 2.

17. The method of any of claims 13-16, wherein two of the metabolic inhibitors are

6-Diazo-5-oxo-L-norleucine (DON) and (2-Deoxy-D-glucose) 2DG.

18. The method of any of claims 13-16, wherein three of the metabolic inhibitors are DON and etomoxir and 2DG.

19. The method of any of claims 13-18, wherein the pathogen is HIV-1.

20. The method of any of claims 13-19, wherein the concentration of each of the metabolic inhibitors is at a suboptimal concentration.

21. A method for measuring the effect of a metabolic inhibitor of metabolic function in cells in a pathogen-infected human comprising:

administering at least one dose of a metabolic inhibitor of metabolic function in cells to the human; and

measuring the level of pathogen infection in the pathogen-infected human.

22. The method of claim 20, wherein measuring the level of pathogen infection in the pathogen-infected human comprises measuring the level of plasma HIV RNA in an HIV-infected human.

23. The method of claim 21, wherein measuring the level of plasma HIV RNA in the HIV-infected human is performed by a reverse transcription and amplification reaction.

24. The method of any of claims 21-23, wherein the level of HIV infection in the human is measured at least 2, 3, 4, 5, 6, 7, 8, 9, or 10 times.

25. A composition for use in the treatment of a pathogen infection in a human, comprising a combination of at least two metabolic inhibitors at a concentration that reduces the production of the pathogen from the pathogen-infected cells.

26. The composition for use according to claim 25, wherein said at least two metabolic inhibitors are of different metabolic functions in the cells.

27. The composition for use according to claim 25 or 26, wherein one of the metabolic inhibitors blocks glycolysis.

28. The composition for use according to any one of claims 25 to 27, wherein one of the metabolic inhibitors blocks fatty acid transport to mitochondria.

29. The composition for use according to any one claims 25 to 28, wherein both of the metabolic inhibitors are selected from Table 1 or Table 2.

30. The composition for use according to any one of claims 25 to 29, wherein two of the metabolic inhibitors are 6-Diazo-5-oxo-L-norleucine (DON) and 2-Deoxy-D-glucose (2DG).

31. The composition for use according to any one of claims 25 to 30, wherein three of the metabolic inhibitors are DON and etomoxir and 2DG.

32. The composition for use according to any one of claims 25 to 31, wherein the concentration of each of the metabolic inhibitors is at a suboptimal concentration.

33. The composition for use according to any one of claims 25 to 32, wherein the pathogen is HIV-1.

34. The composition for use according to any one of claims 25 to 33, wherein the combination of metabolic inhibitors is administered with at least one HIV inhibitor.

35. The composition for use according to claim 34, wherein the combination of metabolic inhibitors is administered with combination antiretroviral therapy.

36. A kit of parts for simultaneous, separate or sequential use in the treatment of a pathogen infection in a human, comprising a combination of metabolic inhibitors as defined in any one of claims 25 to 32.

37. The kit of parts according to claim 36, further comprising at least one HIV inhibitor.

38. The kit of parts according to claim 37, further comprising at least one combination antiretroviral therapy.

PCT/EP2019/086782 2018-12-20 2019-12-20 Cellular metabolism of hiv-1 reservoir seeding in cd4+ t cells WO2020128033A1 (en) Applications Claiming Priority (2) Application Number Priority Date Filing Date Title US201862782400P 2018-12-20 2018-12-20 US62/782,400 2018-12-20 Publications (1) Family ID=69157810 Family Applications (1) Application Number Title Priority Date Filing Date PCT/EP2019/086782 WO2020128033A1 (en) 2018-12-20 2019-12-20 Cellular metabolism of hiv-1 reservoir seeding in cd4+ t cells Country Status (1) Citations (25) * Cited by examiner, † Cited by third party Publication number Priority date Publication date Assignee Title US3710795A (en) 1970-09-29 1973-01-16 Alza Corp Drug-delivery device with stretched, rate-controlling membrane USRE28819E (en) 1972-12-08 1976-05-18 Syntex (U.S.A.) Inc. Dialkylated glycol compositions and medicament preparations containing same US4044126A (en) 1972-04-20 1977-08-23 Allen & Hanburys Limited Steroidal aerosol compositions and process for the preparation thereof US4328245A (en) 1981-02-13 1982-05-04 Syntex (U.S.A.) Inc. Carbonate diester solutions of PGE-type compounds US4358603A (en) 1981-04-16 1982-11-09 Syntex (U.S.A.) Inc. Acetal stabilized prostaglandin compositions US4364923A (en) 1972-04-20 1982-12-21 Allen & Hanburs Limited Chemical compounds US4409239A (en) 1982-01-21 1983-10-11 Syntex (U.S.A.) Inc. Propylene glycol diester solutions of PGE-type compounds US4410545A (en) 1981-02-13 1983-10-18 Syntex (U.S.A.) Inc. Carbonate diester solutions of PGE-type compounds US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme US5333675A (en) 1986-02-25 1994-08-02 Hoffmann-La Roche Inc. Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps US5860957A (en) 1997-02-07 1999-01-19 Sarcos, Inc. Multipathway electronically-controlled drug delivery system US5948433A (en) 1997-08-21 1999-09-07 Bertek, Inc. Transdermal patch US5983134A (en) 1995-04-23 1999-11-09 Electromagnetic Bracing Systems Inc. Electrophoretic cuff apparatus drug delivery system US5985317A (en) 1996-09-06 1999-11-16 Theratech, Inc. Pressure sensitive adhesive matrix patches for transdermal delivery of salts of pharmaceutical agents US6010715A (en) 1992-04-01 2000-01-04 Bertek, Inc. Transdermal patch incorporating a polymer film incorporated with an active agent US6024975A (en) 1992-04-08 2000-02-15 Americare International Diagnostics, Inc. Method of transdermally administering high molecular weight drugs with a polymer skin enhancer US6167301A (en) 1995-08-29 2000-12-26 Flower; Ronald J. Iontophoretic drug delivery device having high-efficiency DC-to-DC energy conversion circuit US6256533B1 (en) 1999-06-09 2001-07-03 The Procter & Gamble Company Apparatus and method for using an intracutaneous microneedle array US6261595B1 (en) 2000-02-29 2001-07-17 Zars, Inc. Transdermal drug patch with attached pocket for controlled heating device US6267983B1 (en) 1997-10-28 2001-07-31 Bando Chemical Industries, Ltd. Dermatological patch and process for producing thereof US9061999B2 (en) 2010-12-15 2015-06-23 Abivax Compounds useful for treating AIDS US9145367B2 (en) 2009-06-12 2015-09-29 Abivax Compounds useful for treating AIDS Patent Citations (29) * Cited by examiner, † Cited by third party Publication number Priority date Publication date Assignee Title US3710795A (en) 1970-09-29 1973-01-16 Alza Corp Drug-delivery device with stretched, rate-controlling membrane US4044126A (en) 1972-04-20 1977-08-23 Allen & Hanburys Limited Steroidal aerosol compositions and process for the preparation thereof US4364923A (en) 1972-04-20 1982-12-21 Allen & Hanburs Limited Chemical compounds US4414209A (en) 1972-04-20 1983-11-08 Allen & Hanburys Limited Micronized aerosol steroids USRE28819E (en) 1972-12-08 1976-05-18 Syntex (U.S.A.) Inc. Dialkylated glycol compositions and medicament preparations containing same US4328245A (en) 1981-02-13 1982-05-04 Syntex (U.S.A.) Inc. Carbonate diester solutions of PGE-type compounds US4410545A (en) 1981-02-13 1983-10-18 Syntex (U.S.A.) Inc. Carbonate diester solutions of PGE-type compounds US4358603A (en) 1981-04-16 1982-11-09 Syntex (U.S.A.) Inc. Acetal stabilized prostaglandin compositions US4409239A (en) 1982-01-21 1983-10-11 Syntex (U.S.A.) Inc. Propylene glycol diester solutions of PGE-type compounds US4683202B1 (en) 1985-03-28 1990-11-27 Cetus Corp US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences US4683195B1 (en) 1986-01-30 1990-11-27 Cetus Corp US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences US5333675A (en) 1986-02-25 1994-08-02 Hoffmann-La Roche Inc. Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme US6010715A (en) 1992-04-01 2000-01-04 Bertek, Inc. Transdermal patch incorporating a polymer film incorporated with an active agent US6024975A (en) 1992-04-08 2000-02-15 Americare International Diagnostics, Inc. Method of transdermally administering high molecular weight drugs with a polymer skin enhancer US5983134A (en) 1995-04-23 1999-11-09 Electromagnetic Bracing Systems Inc. Electrophoretic cuff apparatus drug delivery system US6167301A (en) 1995-08-29 2000-12-26 Flower; Ronald J. Iontophoretic drug delivery device having high-efficiency DC-to-DC energy conversion circuit US5985317A (en) 1996-09-06 1999-11-16 Theratech, Inc. Pressure sensitive adhesive matrix patches for transdermal delivery of salts of pharmaceutical agents US5860957A (en) 1997-02-07 1999-01-19 Sarcos, Inc. Multipathway electronically-controlled drug delivery system US5948433A (en) 1997-08-21 1999-09-07 Bertek, Inc. Transdermal patch US6267983B1 (en) 1997-10-28 2001-07-31 Bando Chemical Industries, Ltd. Dermatological patch and process for producing thereof US6256533B1 (en) 1999-06-09 2001-07-03 The Procter & Gamble Company Apparatus and method for using an intracutaneous microneedle array US6261595B1 (en) 2000-02-29 2001-07-17 Zars, Inc. Transdermal drug patch with attached pocket for controlled heating device US9145367B2 (en) 2009-06-12 2015-09-29 Abivax Compounds useful for treating AIDS US9061999B2 (en) 2010-12-15 2015-06-23 Abivax Compounds useful for treating AIDS Non-Patent Citations (85) * Cited by examiner, † Cited by third party Title "Remington's Pharmaceutical Sciences", 1975, MACK PUBLISHING COMPANY ALLOUCH, A.DAVID, A.AMIE, S.M.LAHOUASSA, H.CHARTIER, L.MARGOTTIN-GOGUET, F.BARRE-SINOUSSI, F.KIM, B.SAEZ-CIRION, A.PANCINO, G.: "p21-mediated RNR2 repression restricts HIV-1 replication in macrophages by inhibiting dNTP biosynthesis pathway", PROC NATL ACAD SCI U S A, vol. 110, 2013, pages E3997 - 4006 AMARA, A.VIDY, A.BOULLA, G.MOLLIER, K.GARCIA-PEREZ, J.ALCAMI, J.BLANPAIN, C.PARMENTIER, M.VIRELIZIER, J.L.CHARNEAU, P. ET AL.: "G protein-dependent CCR5 signaling is not required for efficient infection of primary T lymphocytes and macrophages by R5 human immunodeficiency virus type 1 isolates", J VIROL, vol. 77, 2003, pages 2550 - 2558 AMIE, S.M.NOBLE, E.KIM, B.: "Intracellular nucleotide levels and the control of retroviral infections", VIROLOGY, vol. 436, 2013, pages 247 - 254 ANDERSEN, C.L.JENSEN, J.L.ORNTOFT, T.F.: "Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets", CANCER RES, vol. 64, 2004, pages 5245 - 5250 ANDREA HEGEDUS ET AL: "HIV-1 pathogenicity and virion production are dependent on the metabolic phenotype of activated CD4+ T cells", RETROVIROLOGY, BIOMED CENTRAL LTD., LONDON, GB, vol. 11, no. 1, 25 November 2014 (2014-11-25), pages 98, XP021206215, ISSN: 1742-4690, DOI: 10.1186/S12977-014-0098-4 * ANDRIANTSOANIRINA ET AL., JOURNAL OF MICROBIOLOGICAL METHODS, vol. 78, 2009, pages 165 ASIELLOBAEUMNER, LAB CHIP, vol. 11, no. 8, 2011, pages 1420 - 1430 BANGA, R.PROCOPIO, F.A.NOTO, A.POLLAKIS, G.CAVASSINI, M.OHMITI, K.CORPATAUX, J.M.DE LEVAL, L.PANTALEO, G.PERREAU, M.: "PD-1(+) and follicular helper T cells are responsible for persistent HIV-1 transcription in treated aviremic individuals", NATURE MEDICINE, vol. 22, 2016, pages 754 - 761 BARTON, K.WINCKELMANN, A.PALMER, S.: "HIV-1 Reservoirs During Suppressive Therapy", TRENDS IN MICROBIOLOGY, vol. 24, 2016, pages 345 - 355, XP029515769, DOI: 10.1016/j.tim.2016.01.006 BESNARD, E.HAKRE, S.KAMPMANN, M.LIM, H.W.HOSMANE, N.N.MARTIN, A.BASSIK, M.C.VERSCHUEREN, E.BATTIVELLI, E.CHAN, J. ET AL.: "The mTOR Complex Controls HIV Latency", CELL HOST & MICROBE, vol. 20, 2016, pages 785 - 797, XP029849090, DOI: 10.1016/j.chom.2016.11.001 BURKE, J.D.PLATANIAS, L.C.FISH, E.N.: "Beta Interferon Regulation of Glucose Metabolism Is PI3K/Akt Dependent and Important for Antiviral Activity against Coxsackievirus B3", J VIROL, vol. 88, 2014, pages 3485 - 3495 BUZON, M.J.SUN, H.LI, C.SHAW, A.SEISS, K.OUYANG, Z.MARTIN-GAYO, E.LENG, J.HENRICH, T.J.LI, J.Z. ET AL.: "HIV-1 persistence in CD4+ T cells with stem cell-like properties", NATURE MEDICINE, vol. 20, 2014, pages 139 - 142 BYRNE, J.A.BUTLER, J.L.COOPER, M.D.: "Differential activation requirements for virgin and memory T cells", J IMMUNOL, vol. 141, 1988, pages 3249 - 3257 CALVANESE, V.CHAVEZ, L.LAURENT, T.DING, S.VERDIN, E.: "Dual-color HIV reporters trace a population of latently infected cells and enable their purification", VIROLOGY, vol. 446, 2013, pages 283 - 292, XP055135422, DOI: 10.1016/j.virol.2013.07.037 CHAN, 0.BURKE, J.D.GAO, D.F.FISH, E.N.: "The Chemokine CCL5 Regulates Glucose Uptake and AMP Kinase Signaling in Activated T Cells to Facilitate Chemotaxis", J BIOL CHEM, vol. 287, 2012, pages 29406 - 29416 CHOMONT, N.EL-FAR, M.ANCUTA, P.TRAUTMANN, L.PROCOPIO, F.A.YASSINE-DIAB, B.BOUCHER, G.BOULASSEL, M.R.GHATTAS, G.BRENCHLEY, J.M. ET : "HIV reservoir size and persistence are driven by T cell survival and homeostatic proliferation", NATURE MEDICINE, vol. 15, 2009, pages 893 - 900 CLERET-BUHOT, A.ZHANG, Y.PLANAS, D.GOULET, J.-P.MONTEIRO, P.GOSSELIN, A.WACLECHE, V.S.TREMBLAY, C.L.JENABIAN, M.-A.ROUTY, J.-P. ET: "Identification of novel HIV-1 dependency factors in primary CCR4+CCR6+Thl7 cells via a genome-wide transcriptional approach", RETROVIROLOGY, vol. 12, 2015, pages 102 CROFT, M.BRADLEY, L.M.SWAIN, S.L.: "Naive versus memory CD4 T cell response to antigen. Memory cells are less dependent on accessory cell costimulation and can respond to many antigen-presenting cell types including resting B cells", J IMMUNOL, vol. 152, 1994, pages 2675 - 2685 DAVID, A.SAEZ-CIRION, A.VERSMISSE, P.MALBEC, 0.IANNASCOLI, B.HERSCHKE, F.LUCAS, M.BARRE-SINOUSSI, F.MOUSCADET, J.F.DAERON, M. ET A: "The engagement of activating FcgammaRs inhibits primate lentivirus replication in human macrophages", J IMMUNOL, vol. 177, 2006, pages 6291 - 6300 DEEKS, S.G.LEWIN, S.R.ROSS, A.L.ANANWORANICH, J.BENKIRANE, M.CANNON, P.CHOMONT, N.DOUEK, D.LIFSON, J.D.LO, Y.R. ET AL.: "International AIDS Society global scientific strategy: towards an HIV cure 2016", NATURE MEDICINE, vol. 22, 2016, pages 839 - 850 DESCOURS, B.CRIBIER, A.CHABLE-BESSIA, C.AYINDE, D.RICE, G.CROW, Y.YATIM, A.SCHWARTZ, 0.LAGUETTE, N.BENKIRANE, M.: "SAMHD1 restricts HIV-1 reverse transcription in quiescent CD4(+) T-cells", RETROVIROLOGY, vol. 9, 2012, pages 87, XP021122050, DOI: 10.1186/1742-4690-9-87 E. J. ARTS ET AL: "HIV-1 Antiretroviral Drug Therapy", COLD SPRING HARBOR PERSPECTIVES IN MEDICINE, vol. 2, no. 4, 25 January 2012 (2012-01-25), pages a007161 - a007161, XP055170185, DOI: 10.1101/cshperspect.a007161 * ECKERT ET AL.: "PCR Methods and Applications", vol. 1, 1991, IRL PRESS, OXFORD, pages: 17 FINZI, D.HERMANKOVA, M.PIERSON, T.CARRUTH, L.M.BUCK, C.CHAISSON, R.E.QUINN, T.C.CHADWICK, K.MARGOLICK, J.BROOKMEYER, R. ET AL.: "Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy", SCIENCE, vol. 278, 1997, pages 1295 - 1300, XP002972795, DOI: 10.1126/science.278.5341.1295 FRANKE, E.K.LUBAN, J.: "Inhibition of HIV-1 replication by cyclosporine A or related compounds correlates with the ability to disrupt the Gag-cyclophilin A interaction", VIROLOGY, vol. 222, 1996, pages 2 79 - 2 82 FRITSCH, S.D.WEICHHART, T.: "Effects of Interferons and Viruses on Metabolism", FRONT IMMUNOL, vol. 7, 2016 FROMENTIN, R.BAKEMAN, W.LAWANI, M.B.KHOURY, G.HARTOGENSIS, W.DAFONSECA, S.KILLIAN, M.EPLING, L.HOH, R.SINCLAIR, E. ET AL.: "CD4+ T Cells Expressing PD-1, TIGIT and LAG-3 Contribute to HIV Persistence during ART", PLOS PATHOG, vol. 12, 2016, pages e1005761, XP055620465, DOI: 10.1371/journal.ppat.1005761 GOODWIN, C.M.XU, S.MUNGER, J.: "Stealing the Keys to the Kitchen: Viral Manipulation of the Host Cell Metabolic Network", TRENDS IN MICROBIOLOGY, vol. 23, 2015, pages 789 - 798, XP029327934, DOI: 10.1016/j.tim.2015.08.007 HEGEDUS, A.KAVANAGH WILLIAMSON, M.HUTHOFF, H.: "HIV-1 pathogenicity and virion production are dependent on the metabolic phenotype of activated CD4+ T cells", RETROVIROLOGY, vol. 11, 2014, pages 98, XP021206215, DOI: 10.1186/s12977-014-0098-4 JIA, X.ZHAO, Q.XIONG, Y.: "HIV suppression by host restriction factors and viral immune evasion", CURRENT OPINION IN STRUCTURAL BIOLOGY, vol. 31, 2015, pages 106 - 114 JONES, W.BIANCHI, K.: "Aerobic Glycolysis: Beyond Proliferation", FRONT IMMUNOL, vol. 6, 2015 JOSÉ CARLOS VALLE-CASUSO ET AL: "Cellular Metabolism Is a Major Determinant of HIV-1 Reservoir Seeding in CD4+ T Cells and Offers an Opportunity to Tackle Infection", CELL METABOLISM, vol. 29, no. 3, 1 March 2019 (2019-03-01), United States, pages 611 - 626.e5, XP055685531, ISSN: 1550-4131, DOI: 10.1016/j.cmet.2018.11.015 * JOSÉ LÓPEZ-ALDEGUER ET AL: "NEW CHALLENGES FOR HIV INFECTION New targets and new drugs in the treatment of HIV", ENFERM INFECC MICROBIOL CLIN, vol. 23 (suppl 2), 1 January 2005 (2005-01-01), pages 33 - 40, XP055685591 * KANE, M.ZANG, T.M.RIHN, S.J.ZHANG, F.KUECK, T.ALIM, M.SCHOGGINS, J.RICE, C.M.WILSON, S.J.BIENIASZ, P.D.: "Identification of Interferon-Stimulated Genes with Antiretroviral Activity", CELL HOST MICROBE, vol. 20, 2016, pages 392 - 405, XP029728774, DOI: 10.1016/j.chom.2016.08.005 KAVANAGH WILLIAMSON, M.COOMBES, N.JUSZCZAK, F.ATHANASOPOULOS, M.KHAN, M.B.EYKYN, T.R.SRENATHAN, U.TAAMS, L.S.DIAS ZEIDLER, J.DA PO: "Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1", VIRUSES, vol. 10, 2018 KULKARNI ANURAG ET AL: "Glucose Metabolism and Oxygen Availability Govern Reactivation of the Latent Human Retrovirus HTLV-1", CELL CHEMICAL BIOLOGY, vol. 24, no. 11, 16 November 2017 (2017-11-16), pages 1377, XP085251458, ISSN: 2451-9456, DOI: 10.1016/J.CHEMBIOL.2017.08.016 * KULKARNI, M.M.RATCLIFF, A.N.BHAT, M.ALWARAWRAH, Y.HUGHES, P.ARCOS, J.LOISELLE, D.TORRELLES, J.B.FUNDERBURG, N.T.HAYSTEAD, T.A. ET : "Cellular fatty acid synthase is required for late stages of HIV-1 replication", RETROVIROLOGY, vol. 14, 2017, pages 45 KUMAR, R.FEREZ, M.SWAMY, M.ARECHAGA, I.REJAS, M.T.VALPUESTA, J.M.SCHAMEL, W.W.ALARCON, B.VAN SANTEN, H.M.: "Increased sensitivity of antigen-experienced T cells through the enrichment of oligomeric T cell receptor complexes", IMMUNITY, vol. 35, 2011, pages 375 - 387, XP028298663, DOI: 10.1016/j.immuni.2011.08.010 LANE, A.N.FAN, T.W.M.: "Regulation of mammalian nucleotide metabolism and biosynthesis", NUCLEIC ACIDS RES, vol. 43, 2015, pages 2466 - 2485 LEVER, A.M.JEANG, K.T.: "Insights into cellular factors that regulate HIV-1 replication in human cells", BIOCHEMISTRY, vol. 50, 2011, pages 920 - 931 LI, M.KAO, E.GAO, X.SANDIG, H.LIMMER, K.PAVON-ETERNOD, M.JONES, T.E.LANDRY, S.PAN, T.WEITZMAN, M.D. ET AL.: "Codon-usage-based inhibition of HIV protein synthesis by human schlafen 11", NATURE, vol. 491, 2012, pages 125 - 128 LIM, S.PHILLIPS, J.B.SILVA, L.M.D.ZHOU, M.FODSTAD, 0.OWEN, L.B.TAN, M.: "Interplay between Immune Checkpoint Proteins and Cellular Metabolism", CANCER RES, vol. 77, 2017, pages 1245 - 1249 LOISEL-MEYER, S.SWAINSON, L.CRAVEIRO, M.OBUROGLU, L.MONGELLAZ, C.COSTA, C.MARTINEZ, M.COSSET, F.-L.BATTINI, J.-L.HERZENBERG, L.A. : "Glutl-mediated glucose transport regulates HIV infection", PNAS, vol. 109, 2012, pages 2549 - 2554 LUKIC, Z.DHARAN, A.FRICKE, T.DIAZ-GRIFFERO, F.CAMPBELL, E.M.: "HIV-1 uncoating is facilitated by dynein and kinesin 1", J VIROL, vol. 88, 2014, pages 13613 - 13625 MAN, K.MIASARI, M.SHI, W.XIN, A.HENSTRIDGE, D.C.PRESTON, S.PELLEGRINI, M.BELZ, G.T.SMYTH, G.K.FEBBRAIO, M.A. ET AL.: "The transcription factor IRF4 is essential for TCR affinity-mediated metabolic programming and clonal expansion of T cells", NATURE IMMUNOLOGY, vol. 14, 2013, pages 1155 - 1165 MANEL, N.BATTINI, J.L.SITBON, M.: "Human T cell leukemia virus envelope binding and virus entry are mediated by distinct domains of the glucose transporter GLUT1", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 280, 2005, pages 29025 - 29029 MANEL, N.KIM, F.J.KINET, S.TAYLOR, N.SITBON, M.BATTINI, J.L.: "The ubiquitous glucose transporter GLUT-1 is a receptor for HTLV", CELL, vol. 115, 2003, pages 449 - 459 MANJUSHA M. KULKARNI ET AL: "Cellular fatty acid synthase is required for late stages of HIV-1 replication", RETROVIROLOGY, vol. 14, no. 1, 29 September 2017 (2017-09-29), XP055685508, DOI: 10.1186/s12977-017-0368-z * MATHEWS, C.K.: "Deoxyribonucleotide metabolism, mutagenesis and cancer", NAT REV CANCER, vol. 15, 2015, pages 528 - 539 MATHIEU ANGIN ET AL: "Metabolic plasticity of HIV-specific CD8+ T cells is associated with enhanced antiviral potential and natural control of HIV-1 infection", NATURE METABOLISM, vol. 1, no. 7, 1 July 2019 (2019-07-01), pages 704 - 716, XP055684776, DOI: 10.1038/s42255-019-0081-4 * MATTILA ET AL., NUCLEIC ACIDS RES., vol. 19, 1991, pages 4967 MENDLER, A.N.HU, B.PRINZ, P.U.KREUTZ, M.GOTTFRIED, E.NOESSNER, E.: "Tumor lactic acidosis suppresses CTL function by inhibition of p38 and JNK/c-Jun activation", INT J CANCER, vol. 131, 2012, pages 633 - 640 O'CONNOR, R.S.GUO, L.GHASSEMI, S.SNYDER, N.W.WORTH, A.J.WENG, L.KAM, Y.PHILIPSON, B.TREFELY, S.NUNEZ-CRUZ, S. ET AL.: "The CPTla inhibitor, etomoxir induces severe oxidative stress at commonly used concentrations", SCI REP, vol. 8, 2018, pages 6289 OLIVEIRA, N.M.FARRELL, K.B.EIDEN, M.V.: "In Vitro Characterization of a Koala Retrovirus", J VIROL, vol. 80, 2006, pages 3104 - 3107 PALMER, C.S.DUETTE, G.A.WAGNER, M.C.E.HENSTRIDGE, D.C.SALEH, S.PEREIRA, C.ZHOU, J.SIMAR, D.LEWIN, S.R.OSTROWSKI, M. ET AL.: "Metabolically active CD4+ T cells expressing Glutl and OX40 preferentially harbor HIV during in vitro infection", FEBS LETT PALMER, C.S.OSTROWSKI, M.GOUILLOU, M.TSAI, L.YU, D.ZHOU, J.HENSTRIDGE, D.C.MAISA, A.HEARPS, A.C.LEWIN, S.R. ET AL.: "Increased glucose metabolic activity is associated with Cd4+ T-cell activation and depletion during chronic Hiv infection", AIDS, vol. 28, 2014, pages 297 - 309 PAN, X.BALDAUF, H.M.KEPPLER, O.T.FACKLER, O.T.: "Restrictions to HIV-1 replication in resting CD4+ T lymphocytes", CELL RES, vol. 23, 2013, pages 876 - 885 PASSAES, C.P.BRUEL, T.DECALF, J.DAVID, A.ANGIN, M.MONCEAUX, V.MULLER-TRUTWIN, M.NOEL, N.BOURDIC, K.LAMBOTTE, 0. ET AL.: "Ultrasensitive HIV-1 p24 Assay Detects Single Infected Cells and Differences in Reservoir Induction by Latency Reversal Agents", J VIROL, vol. 91, 2017 PATSOUKIS, N.BARDHAN, K.CHATTERJEE, P.SARI, D.LIU, B.BELL, L.N.KAROLY, E.D.FREEMAN, G.J.PETKOVA, V.SETH, P. ET AL.: "PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation", NATURE COMMUNICATIONS, vol. 6, 2015, pages 6692 PEARCE, E.L.POFFENBERGER, M.C.CHANG, C.H.JONES, R.G.: "Fueling immunity: insights into metabolism and lymphocyte function", SCIENCE, vol. 342, 2013, pages 1242454, XP055558174, DOI: 10.1126/science.1242454 ROEDERER, M.RAJU, P.A.MITRA, D.K.HERZENBERG, L.A.HERZENBERG, L.A.: "HIV does not replicate in naive CD4 T cells stimulated with CD3/CD28", J CLIN INVEST, vol. 99, 1997, pages 1555 - 1564 ROESCH, F.MEZIANE, 0.KULA, A.NISOLE, S.PORROT, F.ANDERSON, I.MAMMANO, F.FASSATI, A.MARCELLO, A.BENKIRANE, M. ET AL.: "Hyperthermia stimulates HIV-1 replication", PLOS PATHOG, vol. 8, 2012, pages el002792 S. LOISEL-MEYER ET AL: "Glut1-mediated glucose transport regulates HIV infection", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 109, no. 7, 30 January 2012 (2012-01-30), US, pages 2549 - 2554, XP055685521, ISSN: 0027-8424, DOI: 10.1073/pnas.1121427109 * SAEZ-CIRION, A.HAMIMI, C.BERGAMASCHI, A.DAVID, A.VERSMISSE, P.MELARD, A.BOUFASSA, F.BARRE-SINOUSSI, F.LAMBOTTE, 0.ROUZIOUX, C. ET : "Restriction of HIV-1 replication in macrophages and CD4+ T cells from HIV controllers", BLOOD, vol. 118, 2011, pages 955 - 964 SAEZ-CIRION, A.SHIN, S.Y.VERSMISSE, P.BARRE-SINOUSSI, F.PANCINO, G.: "Ex vivo T cell-based HIV suppression assay to evaluate HIV-specific CD8+ T-cell responses", NAT PROTOC, vol. 5, 2010, pages 1033 - 1041, XP055244788, DOI: 10.1038/nprot.2010.73 SANCHEZ, E.L.LAGUNOFF, M.: "Viral activation of cellular metabolism", VIROLOGY, vol. 479-480, 2015, pages 609 - 618 SCHNITTMAN, S.M.LANE, H.C.GREENHOUSE, J.JUSTEMENT, J.S.BASELER, M.FAUCI, A.S.: "Preferential infection of CD4+ memory T cells by human immunodeficiency virus type 1: evidence for a role in the selective T-cell functional defects observed in infected individuals", PROC NATL ACAD SCI U S A, vol. 87, 1990, pages 6058 - 6062 SCHOGGINS, J.W.RICE, C.M.: "Interferon-stimulated genes and their antiviral effector functions", CURRENT OPINION IN VIROLOGY, vol. 1, 2011, pages 519 - 525 SEO, J.-Y.YANEVA, R.HINSON, E.R.CRESSWELL, P.: "Human Cytomegalovirus Directly Induces the Antiviral Protein Viperin to Enhance Infectivity", SCIENCE, vol. 332, 2011, pages 1093 - 1097 SHEEHY, A.M.GADDIS, N.C.CHOI, J.D.MALIM, M.H.: "Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein", NATURE, vol. 418, 2002, pages 646 - 650, XP002328600, DOI: 10.1038/nature00939 SHIMODE, S.NAKAOKA, R.SHOGEN, H.MIYAZAWA, T.: "Characterization of feline ASCT1 and ASCT2 as RD-114 virus receptor", JOURNAL OF GENERAL VIROLOGY, vol. 94, 2013, pages 1608 - 1612 SORBARA, L.R.MALDARELLI, F.CHAMOUN, G.SCHILLING, B.CHOKEKIJCAHI, S.STAUDT, L.MITSUYA, H.SIMPSON, I.A.ZEICHNER, S.L.: "Human immunodeficiency virus type 1 infection of H9 cells induces increased glucose transporter expression", J VIROL, vol. 70, 1996, pages 7275 - 7279, XP002166512 STEVENSON, M.STANWICK, T.L.DEMPSEY, M.P.LAMONICA, C.A.: "HIV-1 replication is controlled at the level of T cell activation and proviral integration", EMBO J, vol. 9, 1990, pages 1551 - 1560 SUGIURA, A.RATHMELL, J.C.: "Metabolic Barriers to T Cell Function in Tumors", J IMMUNOL, vol. 200, 2018, pages 400 - 407 TABLER, C.O.LUCERA, M.B.HAQQANI, A.A.MCDONALD, D.J.MIGUELES, S.A.CONNORS, M.TILTON, J.C.: "CD4+ memory stem cells are infected by HIV-1 in a manner regulated in part by SAMHD1 expression", J VIROL, vol. 88, 2014, pages 4976 - 4986 TAKEUCHI, Y.VILE, R.G.SIMPSON, G.O'HARA, B.COLLINS, M.K.WEISS, R.A.: "Feline leukemia virus subgroup B uses the same cell surface receptor as gibbon ape leukemia virus", J VIROL, vol. 66, 1992, pages 1219 - 1222 VALLE-CASUSO, J.C.ALLOUCH, A.DAVID, A.LENZI, G.M.STUDDARD, L.BARRE-SINOUSSI, F.MULLER-TRUTWIN, M.KIM, B.PANCINO, G.SAEZ-CIRION, A.: "p21 Restricts HIV-1 in Monocyte-Derived Dendritic Cells through the Reduction of Deoxynucleoside Triphosphate Biosynthesis and Regulation of SAMHD1 Antiviral Activity", J VIROL, vol. 91, 2017 VAN DER WINDT, G.J.O'SULLIVAN, D.EVERTS, B.HUANG, S.C.BUCK, M.D.CURTIS, J.D.CHANG, C.H.SMITH, A.M.AI, T.FAUBERT, B. ET AL.: "CD8 memory T cells have a bioenergetic advantage that underlies their rapid recall ability", PROC NATL ACAD SCI U S A, vol. 110, 2013, pages 14336 - 14341 VON LAER, D.THOMSEN, S.VOGT, B.DONATH, M.KRUPPA, J.REIN, A.OSTERTAG, W.STOCKING, C.: "Entry of Amphotropic and 10A1 Pseudotyped Murine Retroviruses Is Restricted in Hematopoietic Stem Cell Lines", J VIROL, vol. 72, 1998, pages 1424 - 1430, XP002920843 WAICKMAN, A.T.POWELL, J.D.: "mTOR, metabolism, and the regulation of T-cell differentiation and function", IMMUNOL REV, vol. 249, 2012, pages 43 - 58, XP055399642, DOI: 10.1111/j.1600-065X.2012.01152.x YAO, C.H.LIU, G.Y.WANG, R.MOON, S.H.GROSS, R.W.PATTI, G.J.: "Identifying off-target effects of etomoxir reveals that carnitine palmitoyltransferase I is essential for cancer cell proliferation independent of beta-oxidation", PLOS BIOL, vol. 16, 2018, pages e2003782 ZHANG, J.NUEBEL, E.WISIDAGAMA, D.R.SETOGUCHI, K.HONG, J.S.VAN HORN, C.M.IMAM, S.S.VERGNES, L.MALONE, C.S.KOEHLER, C.M. ET AL.: "Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells", NAT PROTOC, vol. 7, 2012, pages 1068 - 1085, XP055485767, DOI: 10.1038/nprot.2012.048 ZHAO, G.-N.JIANG, D.-S.LI, H.: "Interferon regulatory factors: at the crossroads of immunity, metabolism, and disease", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - MOLECULAR BASIS OF DISEASE, vol. 1852, 2015, pages 365 - 378 ZHAO, Y.BUTLER, E.B.TAN, M.: "Targeting cellular metabolism to improve cancer therapeutics", CELL DEATH & DISEASE, vol. 4, 2013, pages e532, XP055543211, DOI: 10.1038/cddis.2013.60 Similar Documents Publication Publication Date Title Valle-Casuso et al. 2019 Cellular metabolism is a major determinant of HIV-1 reservoir seeding in CD4+ T cells and offers an opportunity to tackle infection Balasubramaniam et al. 2019 Immune control of HIV Chun et al. 1998 Induction of HIV-1 replication in latently infected CD4+ T cells using a combination of cytokines Jekle et al. 2003 In vivo evolution of human immunodeficiency virus type 1 toward increased pathogenicity through CXCR4-mediated killing of uninfected CD4 T cells Mehla et al. 2012 Programming of neurotoxic cofactor CXCL-10 in HIV-1-associated dementia: abrogation of CXCL-10-induced neuro-glial toxicity in vitro by PKC activator JP6280510B2 (en) 2018-02-14 Ingenol derivatives in reactivation of latent HIV virus Del Prete et al. 2014 Effect of suberoylanilide hydroxamic acid (SAHA) administration on the residual virus pool in a model of combination antiretroviral therapy-mediated suppression in SIVmac239-infected indian rhesus macaques Scopelliti et al. 2011 Comparative antiviral activity of integrase inhibitors in human monocyte-derived macrophages and lymphocytes Donahue et al. 2013 Latent HIV-1 can be reactivated by cellular superinfection in a Tat-dependent manner, which can lead to the emergence of multidrug-resistant recombinant viruses Jønsson et al. 2015 Histone deacetylase inhibitor romidepsin inhibits de novo HIV-1 infections Machado Andrade et al. 2019 Host and viral factors influencing interplay between the macrophage and HIV-1 US5977086A (en) 1999-11-02 Method of inhibiting human immunodeficiency virus by combined use of hydroxyurea, a nucleoside analog, and a protease inhibitor WO2010099169A2 (en) 2010-09-02 A novel in vitro hiv-1 latency model for screening reactivation agents of hiv-1 Tyagi et al. 2011 Models of HIV-1 persistence in the CD4+ T cell compartment: past, present and future US10815540B2 (en) 2020-10-27 Treatments to eliminate HIV reservoirs and reduce viral load WO2020128033A1 (en) 2020-06-25 Cellular metabolism of hiv-1 reservoir seeding in cd4+ t cells Shi et al. 2016 A trapping ligand antagonist peptide H22-LP inhibition of human cytomegalovirus infection Thomas 2020 Measuring the HIV-1 Reservoir in the Context of Eradication and Pathogen Co-Infection CN103054867A (en) 2013-04-24 Application of fangchinoline for preparing medicine for treating or preventing HIV US20240226155A1 (en) 2024-07-11 Methods and Products for Reducing HIV Reservoirs Kubota et al. 1998 Novel inhibitory effects of γ-glutamylcysteine ethyl ester against human immunodeficiency virus type 1 production and propagation Mohammadzadeh 2025 HIV Persistence in the Brain Despite Effective Antiretroviral Therapy, Implications for Brain Innate Immune Response and Treatment Bernal Santateresa 2023 A new therapeutical approach to reduce viral persistence, immune activation, and inflammation in people with HIV-1 on antiretroviral therapy WO2024165507A1 (en) 2024-08-15 Use of hif-1α stabilizing agents for the treatment of type i interferonopathies Chang et al. 2024 A First-in-Class Dual Degrader of Bcl-2/Bcl-xL Reverses HIV Latency and Eliminates Ex Vivo Reservoirs Legal Events Date Code Title Description 2020-08-05 121 Ep: the epo has been informed by wipo that ep was designated in this application

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