The invention discloses a detachable and volume adjustable glass slide incubator. A stainless steel incubation frame is pressed on a glass slide, and forms a small inoculation chamber by a rectangular space in the middle by taking the glass slide as the bottom surface together with a silicon gel cushion layer; elastic clampers which clamp the stainless steel incubation frame and the silicon gel cushion layer on the glass slide are arranged additionally; four surfaces of the inner walls of the stainless steel incubation frame and the gel cushion layer are respectively provided with a dovetail groove; the dovetail grooves and incubation chamber space regulating blocks with dovetail-shaped bulges are inserted and matched; The detachable and volume adjustable glass slide incubator provided by the invention is reasonable in structure, guarantees the effect that when an antibody is incubated, all tissue slices pasted on the glass slide can be completely dipped into an antibody incubation liquid by utilizing trace antibody incubation liquid, and further saves the experiment cost of scientific research.
Description Translated from Chinese 坿å¸å¹¶å¯è°å®¹ç§¯çè½½ç»çåµè²å¨Detachable and adjustable volume slide incubatorææ¯é¢å technical field
æ¬åææ¶åä¸ç§å¯æå¸å¹¶å¯è°å®¹ç§¯çè½½ç»çåµè²å¨ã The invention relates to a detachable slide incubator with adjustable volume.
èæ¯ææ¯ Background technique
å¨è¿è¡çå½ç§å¦çç ç©¶ä¸ï¼äººä»¬ç»å¸¸éè¦ä½¿ç¨ç¹å¼æ§å¼ºãçµæåº¦é«çå ç«ç»ç»å妿æ¯å¯¹ç»ç»ç»èçç¹å®ç©è´¨è¿è¡å®ä½åå®éãå ç«ç»ç»å妿æ¯çåºæ¬åçæ¯é¦å å©ç¨å ç«å¦çå·²ç¥æä½ï¼ç¬¬ä¸æä½ï¼ä¸è¢«è§å¯ç»ç»ä¸éè¯å«æåç»åãç±äºå¨ç»ç»ç»èä¸è¿è¡çæåæä½çç»åååºä¸è¬æ¯ä¸å¯è§çï¼éè¦åç¨æ è®°çæ¹æ³å°æç§æ è®°ç©ï¼å¦é ¶ãè§å ç´ ï¼è¿æ¥å°ç¬¬ä¸æä½ä¸ï¼ç¶åç¨ç»ç»å妿¹æ³æ¾ç¤ºæ¤æ è®°ç©æå¨è§å æ¾å¾®éãæ¿å å ±è鿾微éæè¶ é«åè¾¨çæ¾å¾®éä¸è§å¯è§å ç´ ååºçè§å ã使被è§å¯ç»ç»ç»èä¸çæäºç©è´¨å¯è§åãå¨å®ç°ä¸è¿°æå°çç¨æ è®°çæ¹æ³å°æç§æ è®°ç©ï¼å¦é ¶ãè§å ç´ ï¼ç»åå°ç¬¬ä¸æä½ä¸æ¶ï¼äººä»¬å¾å¾éç¨å·²ç»åä¸æç§æ è®°ç©çç¬¬äºæä½ä¸ç¬¬ä¸æä½çç¹å¼æ§ç»åçæ¹æ³ã In life science research, people often need to use immunohistochemical techniques with strong specificity and high sensitivity to locate and quantify specific substances in tissue cells. The basic principle of immunohistochemical technology is to first use known immunological antibodies (primary antibodies) to bind to the antigen to be recognized in the observed tissue. Since the antigen-antibody binding reaction on tissue cells is generally invisible, it is necessary to use a labeling method to link a certain marker (such as an enzyme, fluorescein) to the primary antibody, and then use histochemical methods to display this. label or observe fluorescence from fluorescein under fluorescence microscopy, confocal laser microscopy, or super-resolution microscopy. To visualize certain substances in the cells of the observed tissue. When implementing the above-mentioned labeling method to bind a certain marker (such as an enzyme, fluorescein) to the first antibody, people often use the specificity of the second antibody that has been bound to a certain marker and the first antibody. method of sexual union.
å¨ç¨å ç«ç»ç»å妿æ¯å¯¹ç»ç»ç»èçç¹å®ç©è´¨è¿è¡å®ä½åå®éæ¶ï¼äººä»¬é常éç¨è´´çæè²çæ¹æ³æ¥ä¿æå®éªç»ç»åççè¯å¥½å½¢æåæç¡®åç»ç»åççåå顺åºãè´´çæè²çæ¹æ³çåºæ¬åçæ¯å¨åçæºä¸åä¸ç»ç»çåï¼ç«å³å°è¯¥ç»ç»åçæåç顺åºç²è´´å¨è½½ç»çä¸ãåç»çç»ç»åççå°éãç¬¬ä¸æä½åµè²ãæ´æ¶¤ãç¬¬äºæä½åµè²ãåæ´æ¶¤ãè±æ°´ãéæåå°ç忝å¨è¯¥ç»ç»åçç²è´´å¨è½½ç»çä¸çæ¡ä»¶ä¸è¿è¡çã When immunohistochemical techniques are used to locate and quantify specific substances in tissue cells, patch staining is usually used to maintain the good shape of experimental tissue sections and to clarify the order of each tissue section. The basic principle of the patch staining method is to paste the tissue slices on the glass slide in the order of sectioning immediately after cutting the tissue slices on the microtome. Subsequent blocking of the tissue section, incubation with the first antibody, washing, incubation with the second antibody, re-washing, dehydration, transparency and sealing are all carried out under the condition that the tissue section is pasted on the glass slide.
å¨ä¸è¿°çç»ç»åççå°éãæ´æ¶¤ãåæ´æ¶¤ãè±æ°´ãéæåå°çè¿ç¨ä¸ï¼ç±äºé å¶è¿äºæº¶æ¶²çè¯åææ¬è¾ä½ï¼å 乿´æ¶¤è¿ç¨ä¸ï¼æ´æ¶¤æ¶²çç¨éè¾å¤§ã为äºå¾å°è¯å¥½çå®éªææï¼äººä»¬é叏尿´ä¸ªç²è´´æç»ç»åççè½½ç»çæ¾ç½®å¨ä¸è¿°ç¸å ³æº¶æ¶²ä¸æ¥å¤çãä½å¨è¿è¡ç¬¬ä¸æä½åç¬¬äºæä½åµè²æ¶ï¼ç±äºæä½ä»·æ ¼è¾é«ï¼ç®åä¸è¬çåå éç¬¬ä¸æä½100μlçå®ä»·æ¯3000å ï¼ç¹æ®çåå éçç¬¬ä¸æä½ä»·æ ¼ä¼æ´é«ï¼ï¼ä¸ä½¿ç¨æ¶è½ç¨éçåæ°åä¸è½å¾å¤§ãå æ¤ï¼æä½å°¤å ¶æ¯ç¬¬ä¸æä½æä¸ºå ç«ç»ç»åå¦å®éªææ¬ç主è¦é¨åãæä»¥å¨ç¨å ç«ç»ç»å妿æ¯å¯¹ç»ç»ç»èçç¹å®ç©è´¨è¿è¡å®ä½åå®éæ¶ï¼ä¸è½ç¨å°æ´ä¸ªç²è´´æç»ç»åççè½½ç»çæ¾ç½®å¨æä½åµè²æ¶²ä¸çæ¹æ³æ¥å®æã In the process of sealing, washing, rewashing, dehydration, transparent and sealing of the above-mentioned tissue sections, the cost of reagents for preparing these solutions is relatively low, and in addition, the amount of washing solution is relatively large during the washing process. In order to obtain a good experimental effect, people usually place the entire glass slide pasted with tissue sections in the above-mentioned relevant solution for processing. However, when incubating with the first antibody and the second antibody, due to the high price of the antibody (currently, the price of 100 μl of a general monoclonal primary antibody is 3,000 yuan, and the price of a special monoclonal primary antibody will be higher), and The multiples that can be diluted during use cannot be too large. Therefore, antibodies, especially primary antibodies, become a major part of the cost of immunohistochemistry experiments. Therefore, when immunohistochemical techniques are used to locate and quantify specific substances in tissue cells, it cannot be done by placing the entire glass slide pasted with tissue sections in the antibody incubation solution.
人们å¨è´´çæ³è¿è¡å ç«ç»ç»åå¦æè²æ¶ï¼é常éç¨å°æä½åµè²æ¶²ç´æ¥å å¨è½½ç»ççç»ç»åçä¸çæ¹æ³ãä½ä½¿ç¨è¿ä¸æ¹æ³æ¶ï¼æä½åµè²æ¶²å å¤äºä¼ä¿åä¸äºè漫åºè½½ç»çæµå¤±ï¼å å°äºç»ç»åçä¸çæä½åµè²æ¶²ä¼å¨åµè²çè¿ç¨ä¸ï¼å åµè²æ¶²ä¸æ°´åè¸åèä¸è½ä¿æè½½ç»çä¸çç»ç»åçå§ç»æµ¸æ³¡å¨æä½åµè²æ¶²ä¸ãææ¶ï¼äººä»¬ä¹ä¼ä½¿ç¨å¨è½½ç»çè´´éçç»ç»åçå¨å´ç¨æ²¹æ§ææç»ä¸ä¸ªæ¡çæ¹æ³ï¼å¹¶å¨è¯¥æ²¹æ§ç»æ¡å å æä½åµè²æ¶²ï¼æ¥é²æ¢æä½åµè²æ¶²ç溢åºãä½è¯¥æ¹æ³è½æ¯å°æä½åµè²æ¶²ç´æ¥å å¨è½½ç»çè´´éçç»ç»åçä¸çæ¹æ³ææ¹è¿ä¹å¤ï¼ä½ä½¿ç¨è¯¥æ¹æ³ï¼ç±äºæ²¹æ§ç»æ¡é»æ¢æä½åµè²æ¶²å¤æº¢çè½åå¾å·®ï¼ä¹ä¸è½ä¿è¯å¨æ´ä¸ªæä½åµè²è¿ç¨ä¸ï¼ç»ç»åçå§ç»æµ¸æ³¡å¨æä½åµè²æ¶²ä¸ãèä¸å¨æä½åµè²ãæ´æ¶¤ãè±æ°´ãéæè¿ç¨ä¸ï¼è¿äºæ²¹æ§ææä¼å è±è½è失å»é»æ¢æä½åµè²è¿ç¨ä¸æä½åµè²æ¶²å¤æº¢çä½ç¨ï¼å½åçæ²¹æ§ææè±è½å¹¶ç²éå¨ç»ç»åç䏿¶ï¼ä¼å½±åæç»çåçè§å¯ææï¼èä¸ï¼æ²¹æ§ææå¨ç»ç»åççå¤çè¿ç¨ä¸ï¼è¿ä¼æº¶å°ç¸å ³çå®éªæº¶æ¶²ä¸ï¼æ±¡æå®éªæº¶æ¶²èå½±åçå®éªæº¶æ¶²çéå¤å©ç¨åç»ç»åççå¤çææã When people perform immunohistochemical staining in the patch method, the method of directly adding the antibody incubation solution to the tissue section of the glass slide is usually used. However, when using this method, if the antibody incubation solution is added too much, it will not be preserved and will spill out of the glass slide, and if the antibody incubation solution on the tissue section is added less, it will not be preserved due to the evaporation of water in the incubation solution during the incubation process. Tissue sections on glass slides are always soaked in antibody incubation solution. Sometimes, people also use the method of drawing a frame with oily material around the tissue section attached to the glass slide, and add antibody incubation solution in the oily frame to prevent the antibody incubation solution from overflowing. However, although this method has some improvements over the method of directly adding the antibody incubation solution to the slide-attached tissue section, using this method, due to the poor ability of the oily frame to prevent the antibody incubation solution from spilling, it cannot guarantee During the entire antibody incubation process, the tissue sections were kept soaked in the antibody incubation solution. Moreover, in the process of antibody incubation, washing, dehydration, and transparency, these oily materials will lose the effect of preventing the antibody incubation solution from overflowing during antibody incubation due to falling off; when oily materials fall off and adhere to tissue sections, it will affect the final results. Moreover, during the processing of tissue slices, oily materials will also dissolve into related experimental solutions, polluting the experimental solutions and affecting the reuse of experimental solutions and the processing effect of tissue slices.
åæå 容 Contents of the invention
æ¬åæçç®çå¨äºæä¾ä¸ç§ç»æåçï¼ææçå®éªæ¥éª¤è½æ¹ä¾¿é«æè¿è¡ï¼è½å¾å°è¯å¥½çå ç«ç»ç»å妿è²å®éªç»æç坿å¸å¹¶å¯è°å®¹ç§¯çè½½ç»çåµè²å¨ã The object of the present invention is to provide a detachable and volume-adjustable slide incubator with reasonable structure, convenient and efficient implementation of all experimental steps, and good immunohistochemical staining experimental results.
æ¬åæçææ¯è§£å³æ¹æ¡æ¯ï¼ Technical solution of the present invention is:
ä¸ç§å¯æå¸å¹¶å¯è°å®¹ç§¯çè½½ç»çåµè²å¨ï¼å ¶ç¹å¾æ¯ï¼å æ¬ä¸éé¢åµè²æ¡ï¼ä¸éé¢åµè²æ¡åå¨è½½ç»çä¸ï¼ä¸å ¶ä¸é¢çç¡ è¶å«å±å ±åç±ä¸é´çç©å½¢ç©ºé´ä»¥è½½ç»ç为åºé¢å½¢æåµè²å°å®¤ï¼æè¿°ç¡ è¶å«å±æä¸ä¸éé¢åµè²æ¡ç¸åçä¸é´ç©å½¢ç©ºé´ï¼å¹¶ç²éå¨ä¸éé¢åµè²æ¡ä¸ï¼ä¸è½½ç»çæ¥è§¦ï¼é²æ¢åµè²æ¶²çæ³æ¼ï¼ä¸éé¢åµè²æ¡çå¤å¨å°ºå¯¸åç¡ è¶å«å±çå¤å¨å°ºå¯¸åä¸è½½ç»çå¤å¨å°ºå¯¸ç¸åï¼å¦è®¾æå°ä¸éé¢åµè²æ¡åç¡ è¶å«å±å¤¹æå¨è½½ç»çä¸çå¼¹æ§å¤¹æå¨ï¼æè¿°å¼¹æ§å¤¹æå¨å æ¬å¼¹æ§å¤¹æå¨æ ¹é¨ãå¼¹æ§å¤¹æå¨ç夹æé¨åå¼¹æ§å¤¹æå¨ç夹æå£ï¼å¼¹æ§å¤¹æå¨æ ¹é¨è¿æ¥å¼¹æ§å¤¹æå¨å¤¹æé¨ï¼å¹¶æä¾å¼¹æ§å¤¹æå¨å¨å½¢æè½½ç»çåµè²å¨åæå¸è½½ç»çåµè²å¨æä½æ¶çææä½ç½®ï¼éè¿å¼¹æ§å¤¹æå¨ç夹æå£å°å¼¹æ§å¤¹æå¨å¤¹æå¨åµè²æ¡åè½½ç»çä¸ï¼å¼¹æ§å¤¹æå¨ç夹æé¨å°åµè²æ¡åè½½ç»ç夹æå¨ä¸èµ·å½¢æåµè²å°å®¤ï¼ä¸éé¢åµè²æ¡åç¡ è¶å«å±çå å£åé¢åå«è®¾ç½®ä¸ä¸ªç尾广§½ï¼ç尾广§½ä¸å¸¦æç尾形å¸ä½çåµè²å®¤ç©ºé´è°èåææ¥é åï¼è°èåµè²å®¤ç©ºé´ç大å°ï¼é²æ¢åµè²å®¤ç©ºé´è°èåå¨åµè²å°å®¤å 游走ã A detachable and volume-adjustable slide incubator, which is characterized by: including a stainless steel incubation frame, which is pressed on the slide glass, and together with the silica gel cushion layer below, the rectangular space in the middle is formed by the slide glass. The bottom surface forms an incubation chamber; the silica gel pad has the same middle rectangular space as the stainless steel incubation frame, and adheres to the stainless steel incubation frame to contact with the glass slide to prevent the leakage of the incubation solution; the outer dimension of the stainless steel incubation frame and the silica gel The peripheral dimensions of the cushion layer are all the same as the peripheral dimensions of the slide glass; an elastic holder is provided to clamp the stainless steel incubation frame and the silica gel cushion layer on the glass slide; the elastic holder includes the root of the elastic holder, The clamping part of the elastic holder and the clamping port of the elastic holder; the root of the elastic holder is connected to the clamping part of the elastic holder, and the elastic holder is provided for forming the slide incubator and removing the slide The hand-held position when the incubator is in operation; the elastic holder is clamped on the incubation frame and the glass slide through the clamping port of the elastic clamp, and the clamping part of the elastic clamp clamps the incubation frame and the glass slide on the Together to form an incubation chamber; the stainless steel incubation frame and the inner wall of the silicone cushion are respectively provided with a dovetail groove on the four sides, and the dovetail groove is plugged with the incubation chamber space adjustment block with a dovetail-shaped convex body to adjust the size of the incubation chamber and prevent The incubation chamber space adjustment block moves in the incubation chamber.
ä½¿ç¨æ¹æ³æ¯ï¼å°ä¸éé¢åµè²æ¡åç¡ è¶å«å±åå¨è´´éæç»ç»åççè½½ç»çä¸ï¼ç¡®ä¿ææç»ç»åçé½å¨åµè²æ¡ä¸è½½ç»çå½¢æçåµè²å°å®¤çåºé¨ï¼éæ©åµè²æ¡ä¸è½½ç»çä¸ä¸éå å个边ä¸çä»»æä¸è¾¹ï¼ææå¼¹æ§å¤¹æå¨æ ¹é¨ï¼å°å¼¹æ§å¤¹æå¨ç夹æå£å¯¹ååµè²æ¡ä¸è½½ç»çä¸ä¸ç¸å çç»æï¼å°å¼¹æ§å¤¹æå¨æ¨å ¥åµè²æ¡ä¸è½½ç»çä¸ä¸ç¸å çç»æãä¿è¯å¼¹æ§å¤¹æå¨ç夹æé¨å°åµè²æ¡ä¸è½½ç»çç´§ç´§çåå¨ä¸èµ·ï¼å ¶ä½ä¸è¾¹éå¤ä¸è¿°çå¨ä½å°å¼¹æ§å¤¹æå¨å¤¹å ¥ï¼ç¡®ä¿åµè²æ¡ä¸è½½ç»çç´§å¯æ¥è§¦ï¼å½¢æä¸æ³æ¼çåµè²å°å®¤ï¼å½¢æä¸æ³æ¼çåµè²å°å®¤åï¼æ ¹æ®ç»ç»åçå¨è½½ç»çä¸è´´éçä½ç½®ï¼éæ©æ§å°ååµè²æ¡å ç©å½¢å£ä¸ç4个ç尾广§½ä¸æå ¥1è³4æ ¹åµè²å®¤ç©ºé´è°èåï¼ä»¥çµæ´»è°èåµè²æ¡å ç©å½¢ä»»æä¸è¾¹æå¤è¾¹ç©ºé´ç大å°ï¼è°è好åµè²å®¤ç©ºé´ç大å°åï¼ååµè²å°å®¤ä¸å å ¥ç¬¬ä¸æä½çåµè²æ¶²ï¼ä¿è¯ç²è´´å¨åµè²å°å®¤åºé¨çç»ç»åçè½æµ¸æ³¡å¨å«ç¹å¼ç¬¬ä¸æä½çåµè²æ¶²ä¸ï¼æè¦æ±è¿è¡ç»ç»åççåµè²ï¼ç¬¬ä¸æä½åµè²å®æåï¼å¸åºç¬¬ä¸æä½çåµè²æ¶²ï¼ååºæå ¥åµè²æ¡å ç©å½¢å£ç尾广§½ä¸çåµè²å®¤ç©ºé´è°èåï¼å¸ä¸åµè²æ¡ä¸è½½ç»çä¸ä¸ç¸å çç»æåè¾¹çå¼¹æ§å¤¹æå¨ï¼ä»è½½ç»çä¸å¸ä¸åµè²æ¡ï¼å¯¹ç²è´´å¨è½½ç»çä¸çç»ç»åçè¿è¡æ´æ¶¤å¤çï¼éè¦è¿è¡ç¬¬äºæä½åµè²æ¶ï¼éå¤ä¸è¿°æä½ã The method of use is: Laminate the stainless steel incubation frame and silica gel pad on the glass slide with tissue slices attached to ensure that all tissue slices are at the bottom of the incubation chamber formed by the incubation frame and slide glass; select the incubation frame and slide glass Overlap any one of the four sides up and down, hold the root of the elastic holder, align the clamping mouth of the elastic holder with the structure where the incubation frame and the slide slide are stacked up and down, push the elastic holder into the incubation frame and A structure in which glass slides are stacked on top of each other. Ensure that the clamping part of the elastic holder tightly presses the incubation frame and the glass slide together, and repeat the above-mentioned action on the other three sides to clamp the elastic holder in to ensure that the incubation frame is in close contact with the slide to form an inseparable Leaky incubation chambers; after forming a non-leaking incubation chamber, selectively insert 1 to 4 incubation chamber spaces into the 4 dovetail grooves on the rectangular wall of the incubation frame, depending on where the tissue section is attached on the slide Adjustment block to flexibly adjust the size of any side or polygonal space in the incubation frame; after adjusting the size of the incubation chamber space, add the incubation solution of the primary antibody to the incubation chamber to ensure that the tissue slices pasted on the bottom of the incubation chamber can be soaked In the incubation solution containing the specific first antibody; incubate the tissue slices as required. After the first antibody incubation is completed, suck out the incubation solution of the first antibody, and take out the incubation chamber inserted into the dovetail groove of the rectangular wall in the incubation frame to adjust the space. Block, remove the elastic holders on the four sides of the structure where the incubation frame and the glass slide overlap up and down, remove the incubation frame from the glass slide, and wash the tissue slices pasted on the glass slide; second antibody incubation is required , repeat the above operation.
æ¬åæç»æåçï¼å¨è¿è¡ç»ç»çå ç«ç»ç»åå¦æè²æ¶ï¼å¯éè¿å¤æ¬¡ä½¿ç¨å¼¹æ§å¤¹æå¨å°åµè²æ¡ç´§ç´§çåå¨ç²è´´æç»ç»åççè½½ç»çä¸ï¼å½¢æä¸ä¸ªä¸æ³æ¼çåµè²å°å®¤ãå¹¶éè¿åæä½åµè²å®¤æå ¥æå ¥1è³4æ ¹åµè²å®¤ç©ºé´è°èåçæ¹æ³æ¥çµæ´»è°èæä½åµè²å®¤ä»»æä¸è¾¹æå¤è¾¹ç©ºé´ç大å°ï¼å»é¤åµè²å®¤ä¸å¤ä½ç空é´ï¼å½¢æä¸ä¸ªåµè²ç©ºé´åé并䏿³æ¼çåµè²å°å®¤ãä¿è¯äºå¨è¿è¡æä½åµè²æ¶ï¼ä½¿ç¨æ´å¾®éçæä½åµè²æ¶²ï¼å°±å¾å°äºç²è´´å¨è½½ç»çä¸çææç»ç»åçè½å®å ¨æµ¸æ³¡å¨æä½åµè²æ¶²ä¸çææï¼è¿ä¸æ¥è约äºç§å¦ç ç©¶çå®éªææ¬ãåæ¶ï¼éè¿å¤æ¬¡ååºåµè²å®¤ç©ºé´è°èååå¸ä¸åµè²æ¡ï¼åä¿è¯äºè½½ç»çåå¤çãç»ç»è´´çãæ´æ¶¤ãè±æ°´ãéæãå°ççå®éªæ¥éª¤è½ä¸ååµè²æ¡çå½±åã使ææçå®éªæ¥éª¤è½æ¹ä¾¿é«æè¿è¡ï¼å¹¶æç»è½å¾å°ä¸ä¸ªè¯å¥½çå ç«ç»ç»å妿è²çå®éªç»æã The invention has a reasonable structure, and can tightly press the incubation frame on the glass slide pasted with tissue slices by using the elastic holder multiple times to form a non-leakage incubation chamber when performing immunohistochemical staining of tissues. And by inserting 1 to 4 incubation space adjustment blocks into the antibody incubation chamber to flexibly adjust the size of any side or multilateral space of the antibody incubation chamber, remove the excess space in the incubation chamber, and form an incubation space that is suitable and does not leak Incubation chamber. It is ensured that when antibody incubation is performed, the use of a smaller amount of antibody incubation solution can achieve the effect that all tissue sections pasted on the glass slide can be completely immersed in the antibody incubation solution, further saving the experimental cost of scientific research. At the same time, by taking out the space adjustment block of the incubation chamber and unloading the incubation frame several times, it is ensured that the experimental steps such as slide pretreatment, tissue mounting, washing, dehydration, transparency, and sealing are not affected by the incubation frame. All the experimental steps can be carried out conveniently and efficiently, and finally a good immunohistochemical staining experimental result can be obtained.
éå¾è¯´æ Description of drawings
ä¸é¢ç»åéå¾å宿½ä¾å¯¹æ¬åæä½è¿ä¸æ¥è¯´æã The present invention will be further described below in conjunction with drawings and embodiments.
å¾1æ¯å¯æå¸å¹¶å¯è°å®¹ç§¯çè½½ç»çåµè²å¨çåµè²æ¡ç«ä½ç¤ºæå¾ã Fig. 1 is a three-dimensional schematic diagram of an incubation frame of a detachable slide incubator with adjustable volume.
å¾2æ¯å¯æå¸å¹¶å¯è°å®¹ç§¯çè½½ç»çåµè²å¨çè½½ç»çç«ä½ç¤ºæå¾ã Fig. 2 is a three-dimensional schematic diagram of a slide incubator that can be detachable and has an adjustable volume.
å¾3æ¯å¯æå¸å¹¶å¯è°å®¹ç§¯çè½½ç»çåµè²å¨çå¼¹æ§å¤¹æå¨ç«ä½ç¤ºæå¾ã Fig. 3 is a three-dimensional schematic diagram of the elastic holder of the detachable slide incubator with adjustable volume.
å¾4æ¯å¯æå¸å¹¶å¯è°å®¹ç§¯çè½½ç»çåµè²å¨çåµè²å®¤ç©ºé´è°èåç«ä½ç¤ºæå¾ã Fig. 4 is a three-dimensional schematic diagram of an incubation chamber space adjustment block of a detachable slide incubator with adjustable volume.
å¾5æ¯å¯æå¸å¹¶å¯è°å®¹ç§¯çè½½ç»çåµè²å¨çºµåé¢ç¤ºæå¾ã Fig. 5 is a schematic diagram of a longitudinal section of a detachable and volume-adjustable slide incubator.
å¾ä¸ï¼ In the picture:
1. ä¸éé¢åµè²æ¡ï¼åå¨è½½ç»çä¸ï¼ä¸å ¶ä¸é¢çç¡ è¶å«å±å ±åç±ä¸é´çç©å½¢ç©ºé´ä»¥è½½ç»ç为åºé¢å½¢æåµè²å°å®¤ã 1. Stainless steel incubation frame: Pressed on the glass slide, together with the silicone cushion layer below it, the incubation chamber is formed by the rectangular space in the middle with the glass slide as the bottom surface.
2. ä¸éé¢åµè²æ¡çç¡ è¶å«å±ï¼æä¸ä¸éé¢åµè²æ¡ç¸åçä¸é´ç©å½¢ç©ºé´ï¼å¹¶ç²éå¨ä¸éé¢åµè²æ¡ä¸ï¼å½¢æåµè²å°å®¤æ¶ï¼ä¸è½½ç»çæ¥è§¦ï¼é²æ¢åµè²æ¶²çæ³æ¼ã 2. Silicone gasket of the stainless steel incubation frame: it has the same middle rectangular space as the stainless steel incubation frame, and is adhered to the stainless steel incubation frame. When the incubation chamber is formed, it is in contact with the glass slide to prevent the leakage of the incubation solution.
3. åµè²æ¡å ç©å½¢å£ä¸çç尾广§½ï¼åºå®åµè²å®¤ç©ºé´è°èåï¼é²æ¢åµè²å®¤ç©ºé´è°èåå¨åµè²å°å®¤å 游走ã 3. The dovetail groove on the rectangular wall in the incubation frame: fix the space adjustment block of the incubation room to prevent the space adjustment block from wandering in the incubation chamber.
4. 载ç»çï¼æ¿è½½ç»ç»åçï¼å¹¶ä½ä¸ºåµè²å°å®¤çåºé¢ã 4. Glass slide: it carries tissue slices and serves as the bottom surface of the incubation chamber.
5. 弹æ§å¤¹æå¨æ ¹é¨ï¼è¿æ¥å¼¹æ§å¤¹æå¨å¤¹æé¨ï¼å¹¶æä¾å¼¹æ§å¤¹æå¨å¨å½¢æè½½ç»çåµè²å¨åæå¸è½½ç»çåµè²å¨æ¶çææé¨ä½ã 5. The root of the elastic holder: connect the clamping part of the elastic holder, and provide the holding part of the elastic holder when forming and disassembling the slide incubator.
6 弹æ§å¤¹æå¨ç夹æé¨ï¼å°åµè²æ¡åè½½ç»çåå¨ä¸èµ·å½¢æåµè²å°å®¤ã 6 Clamping part of the elastic holder: press the incubation frame and the glass slide together to form an incubation chamber.
7. 弹æ§å¤¹æå¨ç夹æå£ï¼éè¿è¯¥å¤¹æå£å°å¼¹æ§å¤¹æå¨å¤¹æå¨åµè²æ¡åè½½ç»çä¸ã 7. The clamping port of the elastic holder: the elastic holder is clamped on the incubation frame and the glass slide through the clamping port.
8. åµè²å®¤ç©ºé´è°èåç空é´è°èé¨åï¼æ¾å ¥åµè²å°å®¤ï¼è°èåµè²å®¤ç©ºé´ç大å°ã 8. The space adjustment part of the incubation room space adjustment block: put it into the incubation chamber to adjust the size of the incubation chamber space.
9. åµè²å®¤ç©ºé´è°èåçç尾形å¸ä½ï¼æå ¥åµè²æ¡å å£ä¸çç尾广§½ä¸ï¼é²æ¢åµè²å®¤ç©ºé´è°èåå¨åµè²å°å®¤å 游走ã 9. Dovetail-shaped convex body of the space adjustment block of the incubation chamber: inserted into the dovetail groove on the inner wall of the incubation frame to prevent the space adjustment block of the incubation chamber from wandering in the incubation chamber.
10. åµè²å°å®¤ï¼å½¢æå¾®å°ç空é´åæ¾åµè²æ¶²ï¼åå°æ¢ä½¿ç¨å¾®éçåµè²æ¶²ï¼åä¿è¯äºè½½ç»çä¸çç»ç»åçå§ç»æµ¸æ³¡å¨åµè²æ¶²ä¸ã 10. Incubation chamber: A small space is formed to store the incubation solution, so as to not only use a small amount of incubation solution, but also ensure that the tissue sections on the slide are always immersed in the incubation solution.
11. 弹æ§å¤¹æå¨ã 11. Elastic holder.
å ·ä½å®æ½æ¹å¼ Detailed ways
ä¸ç§å¯æå¸å¹¶å¯è°å®¹ç§¯çè½½ç»çåµè²å¨ï¼å æ¬ä¸éé¢åµè²æ¡ï¼ä¸éé¢åµè²æ¡åå¨è½½ç»çä¸ï¼ä¸å ¶ä¸é¢çç¡ è¶å«å±å ±åç±ä¸é´çç©å½¢ç©ºé´ä»¥è½½ç»ç为åºé¢å½¢æåµè²å°å®¤ï¼æè¿°ç¡ è¶å«å±æä¸ä¸éé¢åµè²æ¡ç¸åçä¸é´ç©å½¢ç©ºé´ï¼å¹¶ç²éå¨ä¸éé¢åµè²æ¡ä¸ï¼ä¸è½½ç»çæ¥è§¦ï¼é²æ¢åµè²æ¶²çæ³æ¼ï¼ä¸éé¢åµè²æ¡çå¤å¨å°ºå¯¸åç¡ è¶å«å±çå¤å¨å°ºå¯¸åä¸è½½ç»çå¤å¨å°ºå¯¸ç¸åï¼å¦è®¾æå°ä¸éé¢åµè²æ¡åç¡ è¶å«å±å¤¹æå¨è½½ç»çä¸çå¼¹æ§å¤¹æå¨ï¼æè¿°å¼¹æ§å¤¹æå¨å æ¬å¼¹æ§å¤¹æå¨æ ¹é¨ãå¼¹æ§å¤¹æå¨ç夹æé¨åå¼¹æ§å¤¹æå¨ç夹æå£ï¼å¼¹æ§å¤¹æå¨æ ¹é¨è¿æ¥å¼¹æ§å¤¹æå¨å¤¹æé¨ï¼å¹¶æä¾å¼¹æ§å¤¹æå¨å¨å½¢æè½½ç»çåµè²å¨åæå¸è½½ç»çåµè²å¨æä½æ¶çææä½ç½®ï¼éè¿å¼¹æ§å¤¹æå¨ç夹æå£å°å¼¹æ§å¤¹æå¨å¤¹æå¨åµè²æ¡åè½½ç»çä¸ï¼å¼¹æ§å¤¹æå¨ç夹æé¨å°åµè²æ¡åè½½ç»ç夹æå¨ä¸èµ·å½¢æåµè²å°å®¤ï¼ä¸éé¢åµè²æ¡åç¡ è¶å«å±çå å£åé¢åå«è®¾ç½®ä¸ä¸ªç尾广§½ï¼ç尾广§½ä¸å¸¦æç尾形å¸ä½çåµè²å®¤ç©ºé´è°èåææ¥é åï¼è°èåµè²å®¤ç©ºé´ç大å°ï¼é²æ¢åµè²å®¤ç©ºé´è°èåå¨åµè²å°å®¤å 游走ã A detachable and volume-adjustable glass slide incubator, including a stainless steel incubation frame, which is pressed on the glass slide, and together with the silica gel pad below it, forms an incubation chamber with a rectangular space in the middle and a glass slide as the bottom surface ; The silica gel pad has the same middle rectangular space as the stainless steel incubation frame, and is adhered to the stainless steel incubation frame to contact with the glass slide to prevent the leakage of the incubation solution; the outer circumference of the stainless steel incubation frame and the outer circumference of the silica gel pad The size is the same as the outer circumference of the glass slide; there is also an elastic holder for clamping the stainless steel incubation frame and the silica gel pad on the glass slide; the elastic holder includes the root of the elastic holder, the elastic holder The clamping part and the clamping mouth of the elastic holder; the root of the elastic holder is connected to the clamping part of the elastic holder, and the elastic holder is provided when the slide incubator is formed and the slide incubator is disassembled. The holding position of the elastic holder; clamp the elastic holder on the incubation frame and the glass slide through the clamping port of the elastic holder, and the clamping part of the elastic holder clamps the incubation frame and the glass slide together to form an incubation chamber A dovetail groove is set on the four sides of the inner wall of the stainless steel incubation frame and the silicone cushion, and the dovetail groove is plugged with the space adjustment block of the incubation room with a dovetail-shaped convex body to adjust the size of the incubation room space and prevent the space adjustment of the incubation room Blocks wander around in the incubation chamber.
æ ¹æ®å ç«ç»ç»åå¦çè¦æ±å¤çéç¨çè½½ç»çï¼ä»¥ä¿è¯ç»ç»åçå¨è½½ç»ç䏿è¯å¥½çç²è´´ææãéåå®éªæéç»ç»è¿è¡åçï¼å¹¶å°å ¶è´´éå¨è½½ç»çä¸ãå°è´´éå¨è½½ç»çä¸çç»ç»åçè¿è¡æä½åµè²åçå°éå¤çã宿ä¸è¿°å®éªæ¥éª¤åï¼å°ä¸éé¢åµè²æ¡ï¼1ï¼åç¡ è¶å«å±ï¼2ï¼åå¨è´´éæç»ç»åççè½½ç»çä¸ï¼4ï¼ï¼ç¡®ä¿ææç»ç»åçé½å¨åµè²æ¡ä¸è½½ç»çå½¢æçåµè²å°å®¤ï¼10ï¼çåºé¨ãéæ©åµè²æ¡ä¸è½½ç»çä¸ä¸éå å个边ä¸çä»»æä¸è¾¹ï¼ææå¼¹æ§å¤¹æå¨æ ¹é¨ï¼5ï¼ï¼å°å¼¹æ§å¤¹æå¨ç夹æå£ï¼7ï¼å¯¹ååµè²æ¡ä¸è½½ç»çä¸ä¸ç¸å çç»æï¼å°å¼¹æ§å¤¹æå¨æ¨å ¥åµè²æ¡ä¸è½½ç»çä¸ä¸ç¸å çç»æãä¿è¯å¼¹æ§å¤¹æå¨ç夹æé¨ï¼6ï¼å°åµè²æ¡ä¸è½½ç»çç´§ç´§çåå¨ä¸èµ·ãå ¶ä½ä¸è¾¹éå¤ä¸è¿°çå¨ä½å°å¼¹æ§å¤¹æå¨ï¼11ï¼å¤¹å ¥ãç¡®ä¿åµè²æ¡ä¸è½½ç»çç´§å¯æ¥è§¦ï¼å½¢æä¸æ³æ¼çåµè²å°å®¤ãå½¢æä¸æ³æ¼çåµè²å°å®¤åï¼æ ¹æ®ç»ç»åçå¨è½½ç»çä¸è´´éçä½ç½®ï¼éæ©æ§å°ååµè²æ¡å ç©å½¢å£ä¸ç4个ç尾广§½ä¸æå ¥1è³4æ ¹åµè²å®¤ç©ºé´è°èåï¼åµè²å®¤ç©ºé´è°èåç±åµè²å®¤ç©ºé´è°èåç空é´è°èé¨åï¼8ï¼åç尾形å¸ä½ï¼9ï¼ç»æï¼ä»¥çµæ´»è°èåµè²æ¡å ç©å½¢ä»»æä¸è¾¹æå¤è¾¹ç©ºé´ç大å°ï¼æå ¥çåµè²å®¤ç©ºé´è°èå以ä¸é®çè´´éäºåµè²å®¤åºé¨çç»ç»åç为æ åãè°è好åµè²å®¤ç©ºé´ç大å°åï¼ååµè²å°å®¤ä¸å å ¥å«ç¹å¼ç¬¬ä¸æä½çåµè²æ¶²ï¼ç¡®ä¿¡ç²è´´å¨åµè²å°å®¤åºé¨çç»ç»åçè½æµ¸æ³¡å¨å«ç¹å¼ç¬¬ä¸æä½çåµè²æ¶²ä¸å³å¯ãæè¦æ±è¿è¡ç»ç»åççåµè²ãç¬¬ä¸æä½åµè²å®æåï¼å¸åºå«ç¹å¼ç¬¬ä¸æä½çåµè²æ¶²ï¼ååºæå ¥åµè²æ¡å ç©å½¢å£ç尾广§½ä¸çåµè²å®¤ç©ºé´è°èåï¼å¸ä¸åµè²æ¡ä¸è½½ç»çä¸ä¸ç¸å çç»æåè¾¹çå¼¹æ§å¤¹æå¨ï¼ä»è½½ç»çä¸å¸ä¸åµè²æ¡ãæå¸¸è§å¯¹ç²è´´å¨è½½ç»çä¸çç»ç»åçè¿è¡æ´æ¶¤å¤çãéè¦è¿è¡ç¬¬äºæä½åµè²æ¶ï¼éå¤ä¸è¿°æä½ï¼å次形æåµè²å®¤ç©ºé´å¤§å°åé并䏿³æ¼çåµè²å°å®¤ï¼å å ¥å«ç¹å¼ç¬¬äºæä½çåµè²æ¶²ï¼å¯¹ç»ç»åçè¿è¡åµè²ãåµè²å®æåï¼å»é¤å«ç¹å¼ç¬¬äºæä½åµè²æ¶²ãéå¤ä¸è¿°æä½æå¸ä¸åµè²æ¡ã对ç²è´´å¨è½½ç»çä¸çç»ç»åçè¿è¡æ´æ¶¤ãè±æ°´ãéæãå°ççåå¤çãææå¤ç宿åï¼å¨æ¾å¾®éä¸è§å¯ç»ç»åççå ç«ç»ç»å妿è²ç»æã Process the selected glass slides according to the requirements of immunohistochemistry to ensure that the tissue sections have a good sticking effect on the glass slides. Select the tissue required for the experiment, slice it, and attach it to a glass slide. The tissue sections attached to the glass slides were blocked before antibody incubation. After completing the above experimental steps, press the stainless steel incubation frame (1) and the silica gel cushion layer (2) on the glass slide with the tissue slice attached (4), to ensure that all tissue sections are incubated in the incubation frame and the glass slide. The bottom of the chamber (10). Select any one of the four sides where the incubation frame overlaps with the glass slide, hold the root of the elastic holder (5), and align the clamping port (7) of the elastic holder with the incubation frame to overlap the slide The structure in which the elastic holder is pushed into the incubator box and the glass slide overlaps up and down. Make sure that the clamping part (6) of the elastic clamp presses the incubation frame and slide tightly together. Repeat the above-mentioned action on the other three sides to clamp the elastic holder (11). Make sure the incubation frame is in tight contact with the slide to create a leak-tight incubation chamber. After forming a non-leaking incubation chamber, according to the position where the tissue section is attached on the glass slide, selectively insert 1 to 4 incubation chamber space adjustment blocks into the 4 dovetail grooves on the rectangular wall of the incubation frame. The space adjustment block is composed of the space adjustment part (8) of the incubation room space adjustment block and the dovetail-shaped convex body (9), so as to flexibly adjust the size of any side or multilateral space of the rectangle in the incubation frame. Covering the tissue sections attached to the bottom of the incubation chamber is standard. After adjusting the size of the incubation chamber space, add the incubation solution containing the specific primary antibody to the incubation chamber, making sure that the tissue slice pasted on the bottom of the incubation chamber can be soaked in the incubation solution containing the specific primary antibody. Incubate tissue sections as required. After the incubation with the primary antibody is completed, suck out the incubation solution containing the specific primary antibody, take out the space adjustment block of the incubation chamber inserted into the dovetail groove on the rectangular wall of the incubation frame, and remove the four sides of the structure that overlaps the incubation frame and the glass slide. Elastic grippers to remove the incubation frame from the slide. Wash the tissue sections pasted on glass slides routinely. When it is necessary to incubate with the second antibody, repeat the above operation to form an incubation chamber with a suitable size and no leakage again, add the incubation solution containing the specific second antibody, and incubate the tissue slices. After the incubation is complete, remove the incubation solution containing the specific secondary antibody. Repeat the above operation to disassemble the lower incubation frame. Post-processing such as washing, dehydration, transparency, and sealing of tissue sections pasted on glass slides. After all treatments were completed, the immunohistochemical staining results of the tissue sections were observed under a microscope.
Claims (1) Translated from Chinese1.ä¸ç§å¯æå¸å¹¶å¯è°å®¹ç§¯çè½½ç»çåµè²å¨ï¼å ¶ç¹å¾æ¯ï¼å æ¬ä¸éé¢åµè²æ¡ï¼ä¸éé¢åµè²æ¡åå¨è½½ç»çä¸ï¼ä¸å ¶ä¸é¢çç¡ è¶å«å±å ±åç±ä¸é´çç©å½¢ç©ºé´ä»¥è½½ç»ç为åºé¢å½¢æåµè²å°å®¤ï¼æè¿°ç¡ è¶å«å±æä¸ä¸éé¢åµè²æ¡ç¸åçä¸é´ç©å½¢ç©ºé´ï¼å¹¶ç²éå¨ä¸éé¢åµè²æ¡ä¸ï¼ä¸è½½ç»çæ¥è§¦ï¼é²æ¢åµè²æ¶²çæ³æ¼ï¼ä¸éé¢åµè²æ¡çå¤å¨å°ºå¯¸åç¡ è¶å«å±çå¤å¨å°ºå¯¸åä¸è½½ç»çå¤å¨å°ºå¯¸ç¸åï¼å¦è®¾æå°ä¸éé¢åµè²æ¡åç¡ è¶å«å±å¤¹æå¨è½½ç»çä¸çå¼¹æ§å¤¹æå¨ï¼æè¿°å¼¹æ§å¤¹æå¨å æ¬å¼¹æ§å¤¹æå¨æ ¹é¨ãå¼¹æ§å¤¹æå¨ç夹æé¨åå¼¹æ§å¤¹æå¨ç夹æå£ï¼å¼¹æ§å¤¹æå¨æ ¹é¨è¿æ¥å¼¹æ§å¤¹æå¨å¤¹æé¨ï¼å¹¶æä¾å¼¹æ§å¤¹æå¨å¨å½¢æè½½ç»çåµè²å¨åæå¸è½½ç»çåµè²å¨æä½æ¶çææä½ç½®ï¼éè¿å¼¹æ§å¤¹æå¨ç夹æå£å°å¼¹æ§å¤¹æå¨å¤¹æå¨åµè²æ¡åè½½ç»çä¸ï¼å¼¹æ§å¤¹æå¨ç夹æé¨å°åµè²æ¡åè½½ç»ç夹æå¨ä¸èµ·å½¢æåµè²å°å®¤ï¼ä¸éé¢åµè²æ¡åç¡ è¶å«å±çå å£åé¢åå«è®¾ç½®ä¸ä¸ªç尾广§½ï¼ç尾广§½ä¸å¸¦æç尾形å¸ä½çåµè²å®¤ç©ºé´è°èåææ¥é åï¼è°èåµè²å®¤ç©ºé´ç大å°ï¼é²æ¢åµè²å®¤ç©ºé´è°èåå¨åµè²å°å®¤å 游走ã1. A detachable and volume-adjustable slide incubator, which is characterized by: including a stainless steel incubation frame, which is pressed on the slide glass, and together with the silica gel pad below it, the rectangular space in the middle is used to carry the glass The bottom surface of the sheet forms an incubation chamber; the silica gel pad has the same middle rectangular space as the stainless steel incubation frame, and is adhered to the stainless steel incubation frame to be in contact with the glass slide to prevent the leakage of the incubation solution; the outer dimension of the stainless steel incubation frame and the outer circumference of the silica gel cushion are the same as the outer circumference of the glass slide; an elastic holder is provided to clamp the stainless steel incubation frame and the silica gel cushion on the glass slide; the elastic holder includes an elastic holder The root, the clamping part of the elastic holder and the clamping mouth of the elastic holder; The hand-held position of the slide incubator during operation; the elastic holder is clamped on the incubation frame and the slide through the clamping port of the elastic clamp, and the clamping part of the elastic clamp clamps the incubation frame and the slide Hold together to form an incubation chamber; the stainless steel incubation frame and the inner wall of the silicone cushion are respectively provided with a dovetail groove on the four sides, and the dovetail groove is plugged with the incubation chamber space adjustment block with a dovetail-shaped convex body to adjust the size of the incubation chamber space , to prevent the space adjustment block of the incubation chamber from wandering in the incubation chamber.
CN201410012882.3A 2014-01-13 2014-01-13 Detachable and volume adjustable glass slide incubator Expired - Fee Related CN103743899B (en) Priority Applications (3) Application Number Priority Date Filing Date Title CN201410012882.3A CN103743899B (en) 2014-01-13 2014-01-13 Detachable and volume adjustable glass slide incubator CN201510052601.1A CN104698163B (en) 2014-01-13 2014-01-13 The using method of the detachable also microslide couveuse of volume adjustable CN201510052659.6A CN104678094B (en) 2014-01-13 2014-01-13 The microslide couveuse of the detachable also volume adjustable of experimental procedure convenience and high-efficiency Applications Claiming Priority (1) Application Number Priority Date Filing Date Title CN201410012882.3A CN103743899B (en) 2014-01-13 2014-01-13 Detachable and volume adjustable glass slide incubator Related Child Applications (2) Application Number Title Priority Date Filing Date CN201510052659.6A Division CN104678094B (en) 2014-01-13 2014-01-13 The microslide couveuse of the detachable also volume adjustable of experimental procedure convenience and high-efficiency CN201510052601.1A Division CN104698163B (en) 2014-01-13 2014-01-13 The using method of the detachable also microslide couveuse of volume adjustable Publications (2) Family ID=50500934 Family Applications (3) Application Number Title Priority Date Filing Date CN201410012882.3A Expired - Fee Related CN103743899B (en) 2014-01-13 2014-01-13 Detachable and volume adjustable glass slide incubator CN201510052601.1A Expired - Fee Related CN104698163B (en) 2014-01-13 2014-01-13 The using method of the detachable also microslide couveuse of volume adjustable CN201510052659.6A Expired - Fee Related CN104678094B (en) 2014-01-13 2014-01-13 The microslide couveuse of the detachable also volume adjustable of experimental procedure convenience and high-efficiency Family Applications After (2) Application Number Title Priority Date Filing Date CN201510052601.1A Expired - Fee Related CN104698163B (en) 2014-01-13 2014-01-13 The using method of the detachable also microslide couveuse of volume adjustable CN201510052659.6A Expired - Fee Related CN104678094B (en) 2014-01-13 2014-01-13 The microslide couveuse of the detachable also volume adjustable of experimental procedure convenience and high-efficiency Country Status (1) Families Citing this family (3) * Cited by examiner, â Cited by third party Publication number Priority date Publication date Assignee Title CN104568557A (en) * 2014-12-22 2015-04-29 ç æµ·è¿ªå°çç©å·¥ç¨æéå ¬å¸ Clamp for fixing slide DE102017213923A1 (en) * 2017-08-10 2019-02-14 Robert Bosch Gesellschaft Für Medizinische Forschung Mbh Device for cultivating tissue sections DE102023201202A1 (en) * 2023-02-14 2024-08-14 Robert Bosch Gesellschaft Für Medizinische Forschung Mbh Device for culturing a tissue section Family Cites Families (9) * Cited by examiner, â Cited by third party Publication number Priority date Publication date Assignee Title US5364790A (en) * 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system CA2330556A1 (en) * 1998-05-13 1999-11-18 Bayer Corporation Optical spectroscopy sample cell JP2002517725A (en) * 1998-06-02 2002-06-18 ã´ã§ã³ã¿ã ã¡ãã£ã«ã« ã·ã¹ãã 㺠ã¤ã³ã³ã¼ãã¬ã¤ããã Method for staining biological specimens by combining unstable reagents on microscope slides CN1138145C (en) * 2001-04-27 2004-02-11 䏿µ·æ¶æ³°çç©ææ¯æéå ¬å¸ Multiple-sample microarray biochip US7238521B2 (en) * 2003-11-24 2007-07-03 Biocept, Inc. Microarray hybridization device having bubble-fracturing elements WO2008002951A2 (en) * 2006-06-29 2008-01-03 Ge Healthcare Bio-Sciences Ab Chamber apparatus CN202433235U (en) * 2012-01-30 2012-09-12 䏿µ·æµ·æ´å¤§å¦ Clamp for fixing glass slides CN203350255U (en) * 2013-07-02 2013-12-18 æ¦æ±ä¸é¹°çç©ææ¯æéå ¬å¸ Adjustable horizontal incubation box CN103344760B (en) * 2013-07-23 2015-08-26 æé½å®é奥éçç©ç§ææéå ¬å¸ A kind of high flux method for immunohistochemical detection and Multi-example SABC check-out consoleGranted publication date: 20150415
Termination date: 20160113
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