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CN103743899B - Detachable and volume adjustable glass slide incubator

CN103743899B - Detachable and volume adjustable glass slide incubator - Google PatentsDetachable and volume adjustable glass slide incubator Download PDF Info
Publication number
CN103743899B
CN103743899B CN201410012882.3A CN201410012882A CN103743899B CN 103743899 B CN103743899 B CN 103743899B CN 201410012882 A CN201410012882 A CN 201410012882A CN 103743899 B CN103743899 B CN 103743899B
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CN
China
Prior art keywords
incubation
frame
glass slide
slide
stainless steel
Prior art date
2014-01-13
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CN201410012882.3A
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Chinese (zh)
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CN103743899A (en
Inventor
彭聿平
邱一华
陈钟
沈卫星
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Nantong University
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Nantong University
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2014-01-13
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2014-01-13
Publication date
2015-04-15
2014-01-13 Application filed by Nantong University filed Critical Nantong University
2014-01-13 Priority to CN201410012882.3A priority Critical patent/CN103743899B/en
2014-01-13 Priority to CN201510052601.1A priority patent/CN104698163B/en
2014-01-13 Priority to CN201510052659.6A priority patent/CN104678094B/en
2014-04-23 Publication of CN103743899A publication Critical patent/CN103743899A/en
2015-04-15 Application granted granted Critical
2015-04-15 Publication of CN103743899B publication Critical patent/CN103743899B/en
Status Expired - Fee Related legal-status Critical Current
2034-01-13 Anticipated expiration legal-status Critical
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The invention discloses a detachable and volume adjustable glass slide incubator. A stainless steel incubation frame is pressed on a glass slide, and forms a small inoculation chamber by a rectangular space in the middle by taking the glass slide as the bottom surface together with a silicon gel cushion layer; elastic clampers which clamp the stainless steel incubation frame and the silicon gel cushion layer on the glass slide are arranged additionally; four surfaces of the inner walls of the stainless steel incubation frame and the gel cushion layer are respectively provided with a dovetail groove; the dovetail grooves and incubation chamber space regulating blocks with dovetail-shaped bulges are inserted and matched; The detachable and volume adjustable glass slide incubator provided by the invention is reasonable in structure, guarantees the effect that when an antibody is incubated, all tissue slices pasted on the glass slide can be completely dipped into an antibody incubation liquid by utilizing trace antibody incubation liquid, and further saves the experiment cost of scientific research.

Description Translated from Chinese 可拆卸并可调容积的载玻片孵育器Detachable and adjustable volume slide incubator

技术领域 technical field

本发明涉及一种可拆卸并可调容积的载玻片孵育器。 The invention relates to a detachable slide incubator with adjustable volume.

背景技术 Background technique

在进行生命科学的研究中,人们经常需要使用特异性强、灵敏度高的免疫组织化学技术对组织细胞的特定物质进行定位和定量。免疫组织化学技术的基本原理是首先利用免疫学的已知抗体(第一抗体)与被观察组织中需识别抗原结合。由于在组织细胞上进行的抗原抗体的结合反应一般是不可见的,需要再用标记的方法将某种标记物(如酶、荧光素)连接到第一抗体上,然后用组织化学方法显示此标记物或在荧光显微镜、激光共聚集显微镜或超高分辨率显微镜下观察荧光素发出的荧光。使被观察组织细胞中的某些物质可视化。在实现上述提到的用标记的方法将某种标记物(如酶、荧光素)结合到第一抗体上时,人们往往采用已结合上某种标记物的第二抗体与第一抗体的特异性结合的方法。 In life science research, people often need to use immunohistochemical techniques with strong specificity and high sensitivity to locate and quantify specific substances in tissue cells. The basic principle of immunohistochemical technology is to first use known immunological antibodies (primary antibodies) to bind to the antigen to be recognized in the observed tissue. Since the antigen-antibody binding reaction on tissue cells is generally invisible, it is necessary to use a labeling method to link a certain marker (such as an enzyme, fluorescein) to the primary antibody, and then use histochemical methods to display this. label or observe fluorescence from fluorescein under fluorescence microscopy, confocal laser microscopy, or super-resolution microscopy. To visualize certain substances in the cells of the observed tissue. When implementing the above-mentioned labeling method to bind a certain marker (such as an enzyme, fluorescein) to the first antibody, people often use the specificity of the second antibody that has been bound to a certain marker and the first antibody. method of sexual union.

在用免疫组织化学技术对组织细胞的特定物质进行定位和定量时,人们通常采用贴片染色的方法来保持实验组织切片的良好形态和明确各组织切片的前后顺序。贴片染色的方法的基本原理是在切片机上切下组织片后,立即将该组织切片按切片顺序粘贴在载玻片上。后续的组织切片的封闭、第一抗体孵育、洗涤、第二抗体孵育、再洗涤、脱水、透明和封片均是在该组织切片粘贴在载玻片上的条件下进行的。 When immunohistochemical techniques are used to locate and quantify specific substances in tissue cells, patch staining is usually used to maintain the good shape of experimental tissue sections and to clarify the order of each tissue section. The basic principle of the patch staining method is to paste the tissue slices on the glass slide in the order of sectioning immediately after cutting the tissue slices on the microtome. Subsequent blocking of the tissue section, incubation with the first antibody, washing, incubation with the second antibody, re-washing, dehydration, transparency and sealing are all carried out under the condition that the tissue section is pasted on the glass slide.

在上述的组织切片的封闭、洗涤、再洗涤、脱水、透明和封片过程中,由于配制这些溶液的试剂成本较低,加之洗涤过程中,洗涤液的用量较大。为了得到良好的实验效果,人们通常将整个粘贴有组织切片的载玻片放置在上述相关溶液中来处理。但在进行第一抗体和第二抗体孵育时,由于抗体价格较高(目前一般的单克隆第一抗体100μl的售价是3000元,特殊的单克隆的第一抗体价格会更高),且使用时能稀释的倍数又不能很大。因此,抗体尤其是第一抗体成为免疫组织化学实验成本的主要部分。所以在用免疫组织化学技术对组织细胞的特定物质进行定位和定量时,不能用将整个粘贴有组织切片的载玻片放置在抗体孵育液中的方法来完成。 In the process of sealing, washing, rewashing, dehydration, transparent and sealing of the above-mentioned tissue sections, the cost of reagents for preparing these solutions is relatively low, and in addition, the amount of washing solution is relatively large during the washing process. In order to obtain a good experimental effect, people usually place the entire glass slide pasted with tissue sections in the above-mentioned relevant solution for processing. However, when incubating with the first antibody and the second antibody, due to the high price of the antibody (currently, the price of 100 μl of a general monoclonal primary antibody is 3,000 yuan, and the price of a special monoclonal primary antibody will be higher), and The multiples that can be diluted during use cannot be too large. Therefore, antibodies, especially primary antibodies, become a major part of the cost of immunohistochemistry experiments. Therefore, when immunohistochemical techniques are used to locate and quantify specific substances in tissue cells, it cannot be done by placing the entire glass slide pasted with tissue sections in the antibody incubation solution.

人们在贴片法进行免疫组织化学染色时,通常采用将抗体孵育液直接加在载玻片的组织切片上的方法。但使用这一方法时,抗体孵育液加多了会保存不了而漫出载玻片流失,加少了组织切片上的抗体孵育液会在孵育的过程中,因孵育液中水分蒸发而不能保持载玻片上的组织切片始终浸泡在抗体孵育液中。有时,人们也会使用在载玻片贴附的组织切片周围用油性材料画一个框的方法,并在该油性画框内加抗体孵育液,来防止抗体孵育液的溢出。但该方法虽比将抗体孵育液直接加在载玻片贴附的组织切片上的方法有改进之处,但使用该方法,由于油性画框阻止抗体孵育液外溢的能力很差,也不能保证在整个抗体孵育过程中,组织切片始终浸泡在抗体孵育液中。而且在抗体孵育、洗涤、脱水、透明过程中,这些油性材料会因脱落而失去阻止抗体孵育过程中抗体孵育液外溢的作用;当发生油性材料脱落并粘附在组织切片上时,会影响最终的切片观察效果;而且,油性材料在组织切片的处理过程中,还会溶到相关的实验溶液中,污染实验溶液而影响的实验溶液的重复利用和组织切片的处理效果。 When people perform immunohistochemical staining in the patch method, the method of directly adding the antibody incubation solution to the tissue section of the glass slide is usually used. However, when using this method, if the antibody incubation solution is added too much, it will not be preserved and will spill out of the glass slide, and if the antibody incubation solution on the tissue section is added less, it will not be preserved due to the evaporation of water in the incubation solution during the incubation process. Tissue sections on glass slides are always soaked in antibody incubation solution. Sometimes, people also use the method of drawing a frame with oily material around the tissue section attached to the glass slide, and add antibody incubation solution in the oily frame to prevent the antibody incubation solution from overflowing. However, although this method has some improvements over the method of directly adding the antibody incubation solution to the slide-attached tissue section, using this method, due to the poor ability of the oily frame to prevent the antibody incubation solution from spilling, it cannot guarantee During the entire antibody incubation process, the tissue sections were kept soaked in the antibody incubation solution. Moreover, in the process of antibody incubation, washing, dehydration, and transparency, these oily materials will lose the effect of preventing the antibody incubation solution from overflowing during antibody incubation due to falling off; when oily materials fall off and adhere to tissue sections, it will affect the final results. Moreover, during the processing of tissue slices, oily materials will also dissolve into related experimental solutions, polluting the experimental solutions and affecting the reuse of experimental solutions and the processing effect of tissue slices.

发明内容 Contents of the invention

本发明的目的在于提供一种结构合理,所有的实验步骤能方便高效进行,能得到良好的免疫组织化学染色实验结果的可拆卸并可调容积的载玻片孵育器。 The object of the present invention is to provide a detachable and volume-adjustable slide incubator with reasonable structure, convenient and efficient implementation of all experimental steps, and good immunohistochemical staining experimental results.

本发明的技术解决方案是: Technical solution of the present invention is:

一种可拆卸并可调容积的载玻片孵育器,其特征是:包括不锈钢孵育框,不锈钢孵育框压在载玻片上,与其下面的硅胶垫层共同由中间的矩形空间以载玻片为底面形成孵育小室;所述硅胶垫层有与不锈钢孵育框相同的中间矩形空间,并粘附在不锈钢孵育框上,与载玻片接触,防止孵育液的泄漏;不锈钢孵育框的外周尺寸及硅胶垫层的外周尺寸均与载玻片外周尺寸相同;另设有将不锈钢孵育框及硅胶垫层夹持在载玻片上的弹性夹持器;所述弹性夹持器包括弹性夹持器根部、弹性夹持器的夹持部及弹性夹持器的夹持口;弹性夹持器根部连接弹性夹持器夹持部,并提供弹性夹持器在形成载玻片孵育器和拆卸载玻片孵育器操作时的手持位置;通过弹性夹持器的夹持口将弹性夹持器夹持在孵育框和载玻片上,弹性夹持器的夹持部将孵育框和载玻片夹持在一起形成孵育小室;不锈钢孵育框及硅胶垫层的内壁四面分别设置一个燕尾凹槽,燕尾凹槽与带有燕尾形凸体的孵育室空间调节块插接配合,调节孵育室空间的大小,防止孵育室空间调节块在孵育小室内游走。 A detachable and volume-adjustable slide incubator, which is characterized by: including a stainless steel incubation frame, which is pressed on the slide glass, and together with the silica gel cushion layer below, the rectangular space in the middle is formed by the slide glass. The bottom surface forms an incubation chamber; the silica gel pad has the same middle rectangular space as the stainless steel incubation frame, and adheres to the stainless steel incubation frame to contact with the glass slide to prevent the leakage of the incubation solution; the outer dimension of the stainless steel incubation frame and the silica gel The peripheral dimensions of the cushion layer are all the same as the peripheral dimensions of the slide glass; an elastic holder is provided to clamp the stainless steel incubation frame and the silica gel cushion layer on the glass slide; the elastic holder includes the root of the elastic holder, The clamping part of the elastic holder and the clamping port of the elastic holder; the root of the elastic holder is connected to the clamping part of the elastic holder, and the elastic holder is provided for forming the slide incubator and removing the slide The hand-held position when the incubator is in operation; the elastic holder is clamped on the incubation frame and the glass slide through the clamping port of the elastic clamp, and the clamping part of the elastic clamp clamps the incubation frame and the glass slide on the Together to form an incubation chamber; the stainless steel incubation frame and the inner wall of the silicone cushion are respectively provided with a dovetail groove on the four sides, and the dovetail groove is plugged with the incubation chamber space adjustment block with a dovetail-shaped convex body to adjust the size of the incubation chamber and prevent The incubation chamber space adjustment block moves in the incubation chamber.

使用方法是:将不锈钢孵育框及硅胶垫层压在贴附有组织切片的载玻片上,确保所有组织切片都在孵育框与载玻片形成的孵育小室的底部;选择孵育框与载玻片上下重叠四个边中的任意一边,手持弹性夹持器根部,将弹性夹持器的夹持口对准孵育框与载玻片上下相叠的结构,将弹性夹持器推入孵育框与载玻片上下相叠的结构。保证弹性夹持器的夹持部将孵育框与载玻片紧紧的压在一起,其余三边重复上述的动作将弹性夹持器夹入,确保孵育框与载玻片紧密接触,形成不泄漏的孵育小室;形成不泄漏的孵育小室后,根据组织切片在载玻片上贴附的位置,选择性地向孵育框内矩形壁上的4个燕尾凹槽中插入1至4根孵育室空间调节块,以灵活调节孵育框内矩形任意一边或多边空间的大小;调节好孵育室空间的大小后,向孵育小室中加入第一抗体的孵育液,保证粘贴在孵育小室底部的组织切片能浸泡在含特异第一抗体的孵育液中;按要求进行组织切片的孵育,第一抗体孵育完成后,吸出第一抗体的孵育液,取出插入孵育框内矩形壁燕尾凹槽中的孵育室空间调节块,卸下孵育框与载玻片上下相叠的结构四边的弹性夹持器,从载玻片上卸下孵育框,对粘贴在载玻片上的组织切片进行洗涤处理;需要进行第二抗体孵育时,重复上述操作。 The method of use is: Laminate the stainless steel incubation frame and silica gel pad on the glass slide with tissue slices attached to ensure that all tissue slices are at the bottom of the incubation chamber formed by the incubation frame and slide glass; select the incubation frame and slide glass Overlap any one of the four sides up and down, hold the root of the elastic holder, align the clamping mouth of the elastic holder with the structure where the incubation frame and the slide slide are stacked up and down, push the elastic holder into the incubation frame and A structure in which glass slides are stacked on top of each other. Ensure that the clamping part of the elastic holder tightly presses the incubation frame and the glass slide together, and repeat the above-mentioned action on the other three sides to clamp the elastic holder in to ensure that the incubation frame is in close contact with the slide to form an inseparable Leaky incubation chambers; after forming a non-leaking incubation chamber, selectively insert 1 to 4 incubation chamber spaces into the 4 dovetail grooves on the rectangular wall of the incubation frame, depending on where the tissue section is attached on the slide Adjustment block to flexibly adjust the size of any side or polygonal space in the incubation frame; after adjusting the size of the incubation chamber space, add the incubation solution of the primary antibody to the incubation chamber to ensure that the tissue slices pasted on the bottom of the incubation chamber can be soaked In the incubation solution containing the specific first antibody; incubate the tissue slices as required. After the first antibody incubation is completed, suck out the incubation solution of the first antibody, and take out the incubation chamber inserted into the dovetail groove of the rectangular wall in the incubation frame to adjust the space. Block, remove the elastic holders on the four sides of the structure where the incubation frame and the glass slide overlap up and down, remove the incubation frame from the glass slide, and wash the tissue slices pasted on the glass slide; second antibody incubation is required , repeat the above operation.

本发明结构合理,在进行组织的免疫组织化学染色时,可通过多次使用弹性夹持器将孵育框紧紧的压在粘贴有组织切片的载玻片上,形成一个不泄漏的孵育小室。并通过向抗体孵育室插入插入1至4根孵育室空间调节块的方法来灵活调节抗体孵育室任意一边或多边空间的大小,去除孵育室中多余的空间,形成一个孵育空间合适并不泄漏的孵育小室。保证了在进行抗体孵育时,使用更微量的抗体孵育液,就得到了粘贴在载玻片上的所有组织切片能完全浸泡在抗体孵育液中的效果,进一步节约了科学研究的实验成本。同时,通过多次取出孵育室空间调节块和卸下孵育框,又保证了载玻片前处理、组织贴片、洗涤、脱水、透明、封片等实验步骤能不受孵育框的影响。使所有的实验步骤能方便高效进行,并最终能得到一个良好的免疫组织化学染色的实验结果。 The invention has a reasonable structure, and can tightly press the incubation frame on the glass slide pasted with tissue slices by using the elastic holder multiple times to form a non-leakage incubation chamber when performing immunohistochemical staining of tissues. And by inserting 1 to 4 incubation space adjustment blocks into the antibody incubation chamber to flexibly adjust the size of any side or multilateral space of the antibody incubation chamber, remove the excess space in the incubation chamber, and form an incubation space that is suitable and does not leak Incubation chamber. It is ensured that when antibody incubation is performed, the use of a smaller amount of antibody incubation solution can achieve the effect that all tissue sections pasted on the glass slide can be completely immersed in the antibody incubation solution, further saving the experimental cost of scientific research. At the same time, by taking out the space adjustment block of the incubation chamber and unloading the incubation frame several times, it is ensured that the experimental steps such as slide pretreatment, tissue mounting, washing, dehydration, transparency, and sealing are not affected by the incubation frame. All the experimental steps can be carried out conveniently and efficiently, and finally a good immunohistochemical staining experimental result can be obtained.

附图说明 Description of drawings

下面结合附图和实施例对本发明作进一步说明。 The present invention will be further described below in conjunction with drawings and embodiments.

图1是可拆卸并可调容积的载玻片孵育器的孵育框立体示意图。 Fig. 1 is a three-dimensional schematic diagram of an incubation frame of a detachable slide incubator with adjustable volume.

图2是可拆卸并可调容积的载玻片孵育器的载玻片立体示意图。 Fig. 2 is a three-dimensional schematic diagram of a slide incubator that can be detachable and has an adjustable volume.

图3是可拆卸并可调容积的载玻片孵育器的弹性夹持器立体示意图。 Fig. 3 is a three-dimensional schematic diagram of the elastic holder of the detachable slide incubator with adjustable volume.

图4是可拆卸并可调容积的载玻片孵育器的孵育室空间调节块立体示意图。 Fig. 4 is a three-dimensional schematic diagram of an incubation chamber space adjustment block of a detachable slide incubator with adjustable volume.

图5是可拆卸并可调容积的载玻片孵育器纵剖面示意图。 Fig. 5 is a schematic diagram of a longitudinal section of a detachable and volume-adjustable slide incubator.

图中: In the picture:

1. 不锈钢孵育框:压在载玻片上,与其下面的硅胶垫层共同由中间的矩形空间以载玻片为底面形成孵育小室。 1. Stainless steel incubation frame: Pressed on the glass slide, together with the silicone cushion layer below it, the incubation chamber is formed by the rectangular space in the middle with the glass slide as the bottom surface.

2. 不锈钢孵育框的硅胶垫层:有与不锈钢孵育框相同的中间矩形空间,并粘附在不锈钢孵育框上,形成孵育小室时,与载玻片接触,防止孵育液的泄漏。 2. Silicone gasket of the stainless steel incubation frame: it has the same middle rectangular space as the stainless steel incubation frame, and is adhered to the stainless steel incubation frame. When the incubation chamber is formed, it is in contact with the glass slide to prevent the leakage of the incubation solution.

3. 孵育框内矩形壁上的燕尾凹槽:固定孵育室空间调节块,防止孵育室空间调节块在孵育小室内游走。 3. The dovetail groove on the rectangular wall in the incubation frame: fix the space adjustment block of the incubation room to prevent the space adjustment block from wandering in the incubation chamber.

4. 载玻片:承载组织切片,并作为孵育小室的底面。 4. Glass slide: it carries tissue slices and serves as the bottom surface of the incubation chamber.

5. 弹性夹持器根部:连接弹性夹持器夹持部,并提供弹性夹持器在形成载玻片孵育器和拆卸载玻片孵育器时的手持部位。 5. The root of the elastic holder: connect the clamping part of the elastic holder, and provide the holding part of the elastic holder when forming and disassembling the slide incubator.

6 弹性夹持器的夹持部:将孵育框和载玻片压在一起形成孵育小室。 6 Clamping part of the elastic holder: press the incubation frame and the glass slide together to form an incubation chamber.

7. 弹性夹持器的夹持口:通过该夹持口将弹性夹持器夹持在孵育框和载玻片上。 7. The clamping port of the elastic holder: the elastic holder is clamped on the incubation frame and the glass slide through the clamping port.

8. 孵育室空间调节块的空间调节部分:放入孵育小室,调节孵育室空间的大小。 8. The space adjustment part of the incubation room space adjustment block: put it into the incubation chamber to adjust the size of the incubation chamber space.

9. 孵育室空间调节块的燕尾形凸体:插入孵育框内壁上的燕尾凹槽中,防止孵育室空间调节块在孵育小室内游走。 9. Dovetail-shaped convex body of the space adjustment block of the incubation chamber: inserted into the dovetail groove on the inner wall of the incubation frame to prevent the space adjustment block of the incubation chamber from wandering in the incubation chamber.

10. 孵育小室:形成微小的空间存放孵育液,做到既使用微量的孵育液,又保证了载玻片上的组织切片始终浸泡在孵育液中。 10. Incubation chamber: A small space is formed to store the incubation solution, so as to not only use a small amount of incubation solution, but also ensure that the tissue sections on the slide are always immersed in the incubation solution.

11. 弹性夹持器。 11. Elastic holder.

具体实施方式 Detailed ways

一种可拆卸并可调容积的载玻片孵育器,包括不锈钢孵育框,不锈钢孵育框压在载玻片上,与其下面的硅胶垫层共同由中间的矩形空间以载玻片为底面形成孵育小室;所述硅胶垫层有与不锈钢孵育框相同的中间矩形空间,并粘附在不锈钢孵育框上,与载玻片接触,防止孵育液的泄漏;不锈钢孵育框的外周尺寸及硅胶垫层的外周尺寸均与载玻片外周尺寸相同;另设有将不锈钢孵育框及硅胶垫层夹持在载玻片上的弹性夹持器;所述弹性夹持器包括弹性夹持器根部、弹性夹持器的夹持部及弹性夹持器的夹持口;弹性夹持器根部连接弹性夹持器夹持部,并提供弹性夹持器在形成载玻片孵育器和拆卸载玻片孵育器操作时的手持位置;通过弹性夹持器的夹持口将弹性夹持器夹持在孵育框和载玻片上,弹性夹持器的夹持部将孵育框和载玻片夹持在一起形成孵育小室;不锈钢孵育框及硅胶垫层的内壁四面分别设置一个燕尾凹槽,燕尾凹槽与带有燕尾形凸体的孵育室空间调节块插接配合,调节孵育室空间的大小,防止孵育室空间调节块在孵育小室内游走。 A detachable and volume-adjustable glass slide incubator, including a stainless steel incubation frame, which is pressed on the glass slide, and together with the silica gel pad below it, forms an incubation chamber with a rectangular space in the middle and a glass slide as the bottom surface ; The silica gel pad has the same middle rectangular space as the stainless steel incubation frame, and is adhered to the stainless steel incubation frame to contact with the glass slide to prevent the leakage of the incubation solution; the outer circumference of the stainless steel incubation frame and the outer circumference of the silica gel pad The size is the same as the outer circumference of the glass slide; there is also an elastic holder for clamping the stainless steel incubation frame and the silica gel pad on the glass slide; the elastic holder includes the root of the elastic holder, the elastic holder The clamping part and the clamping mouth of the elastic holder; the root of the elastic holder is connected to the clamping part of the elastic holder, and the elastic holder is provided when the slide incubator is formed and the slide incubator is disassembled. The holding position of the elastic holder; clamp the elastic holder on the incubation frame and the glass slide through the clamping port of the elastic holder, and the clamping part of the elastic holder clamps the incubation frame and the glass slide together to form an incubation chamber A dovetail groove is set on the four sides of the inner wall of the stainless steel incubation frame and the silicone cushion, and the dovetail groove is plugged with the space adjustment block of the incubation room with a dovetail-shaped convex body to adjust the size of the incubation room space and prevent the space adjustment of the incubation room Blocks wander around in the incubation chamber.

根据免疫组织化学的要求处理选用的载玻片,以保证组织切片在载玻片上有良好的粘贴效果。选取实验所需组织进行切片,并将其贴附在载玻片上。将贴附在载玻片上的组织切片进行抗体孵育前的封闭处理。完成上述实验步骤后,将不锈钢孵育框(1)及硅胶垫层(2)压在贴附有组织切片的载玻片上(4),确保所有组织切片都在孵育框与载玻片形成的孵育小室(10)的底部。选择孵育框与载玻片上下重叠四个边中的任意一边,手持弹性夹持器根部(5),将弹性夹持器的夹持口(7)对准孵育框与载玻片上下相叠的结构,将弹性夹持器推入孵育框与载玻片上下相叠的结构。保证弹性夹持器的夹持部(6)将孵育框与载玻片紧紧的压在一起。其余三边重复上述的动作将弹性夹持器(11)夹入。确保孵育框与载玻片紧密接触,形成不泄漏的孵育小室。形成不泄漏的孵育小室后,根据组织切片在载玻片上贴附的位置,选择性地向孵育框内矩形壁上的4个燕尾凹槽中插入1至4根孵育室空间调节块,孵育室空间调节块由孵育室空间调节块的空间调节部分(8)及燕尾形凸体(9)组成,以灵活调节孵育框内矩形任意一边或多边空间的大小,插入的孵育室空间调节块以不遮盖贴附于孵育室底部的组织切片为标准。调节好孵育室空间的大小后,向孵育小室中加入含特异第一抗体的孵育液,确信粘贴在孵育小室底部的组织切片能浸泡在含特异第一抗体的孵育液中即可。按要求进行组织切片的孵育。第一抗体孵育完成后,吸出含特异第一抗体的孵育液,取出插入孵育框内矩形壁燕尾凹槽中的孵育室空间调节块,卸下孵育框与载玻片上下相叠的结构四边的弹性夹持器,从载玻片上卸下孵育框。按常规对粘贴在载玻片上的组织切片进行洗涤处理。需要进行第二抗体孵育时,重复上述操作,再次形成孵育室空间大小合适并不泄漏的孵育小室,加入含特异第二抗体的孵育液,对组织切片进行孵育。孵育完成后,去除含特异第二抗体孵育液。重复上述操作拆卸下孵育框。对粘贴在载玻片上的组织切片进行洗涤、脱水、透明、封片等后处理。所有处理完成后,在显微镜下观察组织切片的免疫组织化学染色结果。 Process the selected glass slides according to the requirements of immunohistochemistry to ensure that the tissue sections have a good sticking effect on the glass slides. Select the tissue required for the experiment, slice it, and attach it to a glass slide. The tissue sections attached to the glass slides were blocked before antibody incubation. After completing the above experimental steps, press the stainless steel incubation frame (1) and the silica gel cushion layer (2) on the glass slide with the tissue slice attached (4), to ensure that all tissue sections are incubated in the incubation frame and the glass slide. The bottom of the chamber (10). Select any one of the four sides where the incubation frame overlaps with the glass slide, hold the root of the elastic holder (5), and align the clamping port (7) of the elastic holder with the incubation frame to overlap the slide The structure in which the elastic holder is pushed into the incubator box and the glass slide overlaps up and down. Make sure that the clamping part (6) of the elastic clamp presses the incubation frame and slide tightly together. Repeat the above-mentioned action on the other three sides to clamp the elastic holder (11). Make sure the incubation frame is in tight contact with the slide to create a leak-tight incubation chamber. After forming a non-leaking incubation chamber, according to the position where the tissue section is attached on the glass slide, selectively insert 1 to 4 incubation chamber space adjustment blocks into the 4 dovetail grooves on the rectangular wall of the incubation frame. The space adjustment block is composed of the space adjustment part (8) of the incubation room space adjustment block and the dovetail-shaped convex body (9), so as to flexibly adjust the size of any side or multilateral space of the rectangle in the incubation frame. Covering the tissue sections attached to the bottom of the incubation chamber is standard. After adjusting the size of the incubation chamber space, add the incubation solution containing the specific primary antibody to the incubation chamber, making sure that the tissue slice pasted on the bottom of the incubation chamber can be soaked in the incubation solution containing the specific primary antibody. Incubate tissue sections as required. After the incubation with the primary antibody is completed, suck out the incubation solution containing the specific primary antibody, take out the space adjustment block of the incubation chamber inserted into the dovetail groove on the rectangular wall of the incubation frame, and remove the four sides of the structure that overlaps the incubation frame and the glass slide. Elastic grippers to remove the incubation frame from the slide. Wash the tissue sections pasted on glass slides routinely. When it is necessary to incubate with the second antibody, repeat the above operation to form an incubation chamber with a suitable size and no leakage again, add the incubation solution containing the specific second antibody, and incubate the tissue slices. After the incubation is complete, remove the incubation solution containing the specific secondary antibody. Repeat the above operation to disassemble the lower incubation frame. Post-processing such as washing, dehydration, transparency, and sealing of tissue sections pasted on glass slides. After all treatments were completed, the immunohistochemical staining results of the tissue sections were observed under a microscope.

Claims (1) Translated from Chinese

1.一种可拆卸并可调容积的载玻片孵育器,其特征是:包括不锈钢孵育框,不锈钢孵育框压在载玻片上,与其下面的硅胶垫层共同由中间的矩形空间以载玻片为底面形成孵育小室;所述硅胶垫层有与不锈钢孵育框相同的中间矩形空间,并粘附在不锈钢孵育框上,与载玻片接触,防止孵育液的泄漏;不锈钢孵育框的外周尺寸及硅胶垫层的外周尺寸均与载玻片外周尺寸相同;另设有将不锈钢孵育框及硅胶垫层夹持在载玻片上的弹性夹持器;所述弹性夹持器包括弹性夹持器根部、弹性夹持器的夹持部及弹性夹持器的夹持口;弹性夹持器根部连接弹性夹持器夹持部,并提供弹性夹持器在形成载玻片孵育器和拆卸载玻片孵育器操作时的手持位置;通过弹性夹持器的夹持口将弹性夹持器夹持在孵育框和载玻片上,弹性夹持器的夹持部将孵育框和载玻片夹持在一起形成孵育小室;不锈钢孵育框及硅胶垫层的内壁四面分别设置一个燕尾凹槽,燕尾凹槽与带有燕尾形凸体的孵育室空间调节块插接配合,调节孵育室空间的大小,防止孵育室空间调节块在孵育小室内游走。1. A detachable and volume-adjustable slide incubator, which is characterized by: including a stainless steel incubation frame, which is pressed on the slide glass, and together with the silica gel pad below it, the rectangular space in the middle is used to carry the glass The bottom surface of the sheet forms an incubation chamber; the silica gel pad has the same middle rectangular space as the stainless steel incubation frame, and is adhered to the stainless steel incubation frame to be in contact with the glass slide to prevent the leakage of the incubation solution; the outer dimension of the stainless steel incubation frame and the outer circumference of the silica gel cushion are the same as the outer circumference of the glass slide; an elastic holder is provided to clamp the stainless steel incubation frame and the silica gel cushion on the glass slide; the elastic holder includes an elastic holder The root, the clamping part of the elastic holder and the clamping mouth of the elastic holder; The hand-held position of the slide incubator during operation; the elastic holder is clamped on the incubation frame and the slide through the clamping port of the elastic clamp, and the clamping part of the elastic clamp clamps the incubation frame and the slide Hold together to form an incubation chamber; the stainless steel incubation frame and the inner wall of the silicone cushion are respectively provided with a dovetail groove on the four sides, and the dovetail groove is plugged with the incubation chamber space adjustment block with a dovetail-shaped convex body to adjust the size of the incubation chamber space , to prevent the space adjustment block of the incubation chamber from wandering in the incubation chamber.

CN201410012882.3A 2014-01-13 2014-01-13 Detachable and volume adjustable glass slide incubator Expired - Fee Related CN103743899B (en) Priority Applications (3) Application Number Priority Date Filing Date Title CN201410012882.3A CN103743899B (en) 2014-01-13 2014-01-13 Detachable and volume adjustable glass slide incubator CN201510052601.1A CN104698163B (en) 2014-01-13 2014-01-13 The using method of the detachable also microslide couveuse of volume adjustable CN201510052659.6A CN104678094B (en) 2014-01-13 2014-01-13 The microslide couveuse of the detachable also volume adjustable of experimental procedure convenience and high-efficiency Applications Claiming Priority (1) Application Number Priority Date Filing Date Title CN201410012882.3A CN103743899B (en) 2014-01-13 2014-01-13 Detachable and volume adjustable glass slide incubator Related Child Applications (2) Application Number Title Priority Date Filing Date CN201510052659.6A Division CN104678094B (en) 2014-01-13 2014-01-13 The microslide couveuse of the detachable also volume adjustable of experimental procedure convenience and high-efficiency CN201510052601.1A Division CN104698163B (en) 2014-01-13 2014-01-13 The using method of the detachable also microslide couveuse of volume adjustable Publications (2) Family ID=50500934 Family Applications (3) Application Number Title Priority Date Filing Date CN201410012882.3A Expired - Fee Related CN103743899B (en) 2014-01-13 2014-01-13 Detachable and volume adjustable glass slide incubator CN201510052601.1A Expired - Fee Related CN104698163B (en) 2014-01-13 2014-01-13 The using method of the detachable also microslide couveuse of volume adjustable CN201510052659.6A Expired - Fee Related CN104678094B (en) 2014-01-13 2014-01-13 The microslide couveuse of the detachable also volume adjustable of experimental procedure convenience and high-efficiency Family Applications After (2) Application Number Title Priority Date Filing Date CN201510052601.1A Expired - Fee Related CN104698163B (en) 2014-01-13 2014-01-13 The using method of the detachable also microslide couveuse of volume adjustable CN201510052659.6A Expired - Fee Related CN104678094B (en) 2014-01-13 2014-01-13 The microslide couveuse of the detachable also volume adjustable of experimental procedure convenience and high-efficiency Country Status (1) Families Citing this family (3) * Cited by examiner, † Cited by third party Publication number Priority date Publication date Assignee Title CN104568557A (en) * 2014-12-22 2015-04-29 珠海迪尔生物工程有限公司 Clamp for fixing slide DE102017213923A1 (en) * 2017-08-10 2019-02-14 Robert Bosch Gesellschaft Für Medizinische Forschung Mbh Device for cultivating tissue sections DE102023201202A1 (en) * 2023-02-14 2024-08-14 Robert Bosch Gesellschaft Für Medizinische Forschung Mbh Device for culturing a tissue section Family Cites Families (9) * Cited by examiner, † Cited by third party Publication number Priority date Publication date Assignee Title US5364790A (en) * 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system CA2330556A1 (en) * 1998-05-13 1999-11-18 Bayer Corporation Optical spectroscopy sample cell JP2002517725A (en) * 1998-06-02 2002-06-18 ヴェンタナ メディカル システムズ インコーポレイテッド Method for staining biological specimens by combining unstable reagents on microscope slides CN1138145C (en) * 2001-04-27 2004-02-11 上海晶泰生物技术有限公司 Multiple-sample microarray biochip US7238521B2 (en) * 2003-11-24 2007-07-03 Biocept, Inc. 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