Bioremediation of oil spillage in soils using consortia of microbes beckons much exploration. The present study involves bioremediation of oil-contaminated soils from north Chennai, India, using indigenous microbial consortia. Totally, 32 positive oil degrading isolates were obtained from 3 different locations, i.e., petrol filling stations, automobile workshops and oil refineries. Substrate utilization patterns of individual isolates and the consortial sets were observed. Mixture of three common hydrocarbons (petrol, diesel and engine oil) was used for studies. The substrate oil utilized by consortia was taken for thin-layer and column chromatography which perfectly resulted in varied fractions of oil compared to the unused oil as control. The best consortia were used directly for bioremediation experiment. Three different oil-contaminated soils were used and bioremediation patterns were observed. The rate of bioremediation differed within soils, nevertheless all soils were almost 100 % reclaimed within 30 days. Bioremediation kinetics showed that the process corresponds to first-order kinetics and kinetic constants for the different soils ranged from 0.051 to 0.077/day. Assessment of detoxification of acute phytotoxicity owing to the pollutant oil was done, and results observed were significant. An increase of 25, 300 and 212 % in germination index, average growth index and sustenance index, respectively, of Trigonella foenum-graecum Linn. in treated soils was observed, compared to untreated soils. Thus, this study confirmed that microbes in ‘Consortial Union’ serve as better treating agents in bioremediation of oil-contaminated soils than individual microorganisms.
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We express our sincere thanks to Dr. I. Seethalakshmi, Director, Life Teck Research Centre, Chennai for proving facilities and support for successful completion of the project.
Author information Authors and AffiliationsLife Teck Research Centre, Vadapalani, Chennai, 26, India
S. Shankar, C. Kansrajh, M. G. Dinesh & R. S. Satyan
Department of Microbiology, Cauvery College for Women, Trichy, 18, India
S. Kiruthika
Department of Microbial Biotechnology, Bharathiar University, Coimbatore, 46, India
A. Tharanipriya
Correspondence to S. Shankar.
About this article Cite this articleShankar, S., Kansrajh, C., Dinesh, M.G. et al. Application of indigenous microbial consortia in bioremediation of oil-contaminated soils. Int. J. Environ. Sci. Technol. 11, 367–376 (2014). https://doi.org/10.1007/s13762-013-0366-1
Received: 09 August 2011
Revised: 01 February 2012
Accepted: 25 January 2013
Published: 20 September 2013
Issue Date: March 2014
DOI: https://doi.org/10.1007/s13762-013-0366-1
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