Home - News ( United States | United Kingdom | Italy | Germany ) - Football scores

Showing content from https://link.springer.com/doi/10.1007/s11356-012-1205-5 below:

Arsenite tolerance in rice (Oryza sativa L.) involves coordinated role of metabolic pathways of thiols and amino acids

Thiolic ligands and several amino acids (AAs) are known to build up in plants against heavy metal stress. In the present study, alteration of various AAs in rice and its synchronized role with thiolic ligand was explored for arsenic (As) tolerance and detoxification. To understand the mechanism of As tolerance and stress response, rice seedlings of one tolerant (Triguna) and one sensitive (IET-4786) cultivar were exposed to arsenite (0–25 μM) for 7 days for various biochemical analyses using spectrophotometer, HPLC and ICPMS. Tolerant and sensitive cultivars respond differentially in terms of thiol metabolism, essential amino acids (EEAs) and nonessential amino acids (NEEAs) vis-á-vis As accumulation. Thiol biosynthesis-related enzymes were positively correlated to As accumulation in Triguna. Conversely, these enzymes, cysteine content and GSH/GSSG ratio declined significantly in IET-4786 upon As exposure. The level of identified phytochelatin (PC) species (PC₂, PC₃ and PC₄) and phytochelatin synthase activity were also more pronounced in Triguna than IET-4786. Nearly all EAAs were negatively affected by As-induced oxidative stress (except phenylalanine in Triguna), but more significantly in IET-4786 than Triguna. However, most of the stress-responsive NEAAs like glutamic acid, histidine, alanine, glycine, tyrosine, cysteine and proline were enhanced more prominently in Triguna than IET-4786 upon As exposure. The study suggests that IET-4786 appears sensitive to As due to reduction of AAs and thiol metabolic pathway. However, a coordinated response of thiolic ligands and stress-responsive AAs seems to play role for As tolerance in Triguna to achieve the effective complexation of As by PCs.

This is a preview of subscription content, log in via an institution to check access.

Springer+ Basic

€34.99 /Month

• Get 10 units per month
• Download Article/Chapter or eBook
• 1 Unit = 1 Article or 1 Chapter
• Cancel anytime

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Ala:

Alanine

Arg:

Arginine

As:

Arsenic

As^III :

Arsenite

Asp:

Aspartic acid

CS:

Cysteine synthase

Cys:

Cysteine

EEAs:

Essential amino acids

Glu:

Glutamic acid

GSH:

Reduced glutathione

GSSG:

Oxidized glutathione

GST:

Glutathione-S-transferase

γ-ECS:

γ-Glutamyl cysteine synthase

γ-GT:

γ-Glutamyl transpeptidase

Gly:

Glycine

His:

Histidine

Ile:

Isoleucine

Leu:

Leucine

Lys:

Lysine

MDA:

Malondialdehyde

Met:

Methionine

NEEAs:

Non-essential amino acids

PC:

Phytochelatin

PCS:

Phytochelatin synthase

Phe:

Phenylalanine

Pro:

Proline

ROS:

Reactive oxygen species

SAT:

Serine acetyl transferase

Ser:

Serine

Thr:

Threonine

Tyr:

Tyrosine

Val:

Valine

• Bidlingmeyer BA, Cohen SA, Tarvin TL (1984) Rapid analysis of amino acids using pre-column derivatization. J Chromatogr 336:93–104

  Article  CAS  Google Scholar 

• Blaszczyk A, Sirko L, Hawkesford MJ, Sirko A (2002) Biochemical analysis of transgenic tobacco lines producing bacterial serine acetyltransferase. Plant Sci 162:589–597

  Article  CAS  Google Scholar 

• Bleeker PM, Hakvoort HWJ, Bliek M, Souer E, Schat H (2006) Enhanced arsenate reduction by a CDC25-like tyrosine phosphatase explains increased phytochelatin accumulation in arsenate-tolerant Holcus lanatus. Plant J 45:917–929

  Article  CAS  Google Scholar 

• Dixon R, Paiva NL (1995) Stress-induced phenylopropanoid metabolism. Plant Cell 7:1085

  CAS  Google Scholar 

• Dwivedi S, Tripathi RD, Srivastava S et al (2010a) Effect of arsenate exposure on amino acids, mineral nutrient status and antioxidant in rice (Oryza sativa L.) genotypes. Environ Sci Technol 44:9542–9549

  Article  CAS  Google Scholar 

• Dwivedi S, Tripathi RD, Tripathi P et al (2010b) Arsenic affects mineral nutrients in grains of various Indian rice (Oryza sativa L.) genotypes grown under arsenic-contaminated soils of West Bengal. Protoplasma. doi:10.1007/s00709-010-0151-7

• Dwivedi S, Mishra A, Tripathi P et al (2012) Arsenic affects essential and non-essential amino acids differentially in rice grains: inadequacy of amino acids in rice based diet. Environ Int 46:16–22

  Article  CAS  Google Scholar 

• Gebicki S, Gebicki JM (1993) Formation of peroxides in amino acids and proteins exposed to oxygen free radicals. Biochem J 289:743–749

  CAS  Google Scholar 

• Habig WH, Jacoby WB (1981) Assay for differentiation of glutathione S-transferases. Meth Enzymol 77:398–405

  Article  CAS  Google Scholar 

• Hartley-Whitaker J, Ainsworth G, Vooijs R et al (2001) Phytochelatins are involved in differential arsenate tolerance in Holcus lanatus L. Plant Physiol 126:299–306

  Article  CAS  Google Scholar 

• Hissin PJ, Hilf R (1976) A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem 74:214–226

  Article  CAS  Google Scholar 

• Krämer U, Cotter-Howells JD, Charnock JM, Baker AJM, Smith JAC (1996) Free histidine as a metal chelator in plants that accumulate nickel. Nature 379:635–638

  Article  Google Scholar 

• Liu WJ, Zhu YG, Smith FA, Smith SE (2004) Do phosphorus nutrition and iron plaque alter arsenate (As) uptake by rice seedlings in hydroponic culture? New Phytol 162:481–488

  Article  CAS  Google Scholar 

• Liu WJ, Wood BA, Raab A, McGrath SP, Zhao FJ, Feldmann J (2010) Complexation of arsenite with phytochelatins reduces arsenite efflux and translocation from roots to shoots in Arabidopsis ^1[W]. Plant Physiol 152:2211–2221

  Article  CAS  Google Scholar 

• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265–275

  CAS  Google Scholar 

• Ma JF, Yamaji N, Mitani N, Xu XY, Su YH et al (2008) Transporters of arsenite in rice and their role in arsenic accumulation in rice grain. Proc Natl Acad Sci U S A 105:9931–9935

  Article  CAS  Google Scholar 

• Meharg AA, Hartley-Whitaker J (2002) Arsenic uptake and metabolism in arsenic resistant and nonresistant plant species. New Phytol 154:29–43

  Article  CAS  Google Scholar 

• Minocha R, Thangavel P, Dhankher OP, Long S (2008) Separation and quantification of monothiols and phytochelatins from a wide variety of cell cultures and tissues of trees and other plants using high performance liquid chromatography. J Chromatogr 1207:72–83

  Article  CAS  Google Scholar 

• Mishra S, Dubey RS (2006) Inhibition of ribonuclease and protease activities in arsenic exposed rice seedlings: role of proline as enzyme protectant. J Plant Physiol 163:927–936

  Article  CAS  Google Scholar 

• Mishra S, Srivastava S, Tripathi RD, Trivedi PK (2008) Thiol metabolism and antioxidant system complement each other during arsenate detoxification in Ceratophyllum demersum L. Aquat Toxicol 86:205–215

  Article  CAS  Google Scholar 

• Mondal P, Majumdar CB, Mohanty B (2006) Laboratory based approaches for arsenic remediation from contaminated water: recent developments. J Hazard Mater 137:464–479

  Article  CAS  Google Scholar 

• Moons A (2003) Osgstu3 and Osgstu4, encoding tau class glutathione S-transferases, are heavy metal- and hypoxic stress-induced and differentially salt stress-responsive in rice roots. FEBS Lett 553:427–432

  Article  CAS  Google Scholar 

• Nakano Y, Sekiya J (2005) γ-glutamyltransferase and glutathione catabolism in Raphanus sativus L. In: Saito LJ, De Kok LJ, Stulen I, Hawkesford MJ, Schnug E, Sirko A, Renneberg H (eds) Sulfur transport and assimilation in plants in the post genomic era. Backhuys Publishers, Leiden, pp 107–110

  Google Scholar 

• Norton GJ, Lou-Hing DE, Meharg AA, Price AH (2008) Rice–arsenate interactions in hydroponics: whole genome transcriptional analysis. J Exp Bot 59:2267–2276

  Article  CAS  Google Scholar 

• Norton GJ, Islam MR, Deacon CM et al (2009) Identification of low inorganic and total grain arsenic rice cultivars from Bangladesh. Environ Sci Technol 43:6070–6075

  Article  CAS  Google Scholar 

• Orlowski M, Meister A (1973) γ-Glutamyl cyclotransferase distribution, isozymic forms, and specificity. J Biol Chem 248:2836–2844

  CAS  Google Scholar 

• Pavlik M, Pavlikova D, Staszkova L et al (2010) The effect of arsenic contamination on amino acids metabolism in Spinacia oleracea L. Ecotoxicol Environ Safe 73:1309–1313

  Article  CAS  Google Scholar 

• Rai A, Tripathi P, Dwivedi S et al (2011) Arsenic tolerances in rice (Oryza sativa) have a predominant role in transcriptional regulation of a set of genes including sulphur assimilation pathway and antioxidant system. Chemosphere 82:986–995

  Article  Google Scholar 

• Rother M, Krauss GJ, Grass G, Wesenberg D (2008) Sulphate assimilation under Cd²⁺ stress in Physcomitrella patens-combined transcript, enzyme and metabolite profiling. Plant Cell Environ 29:1801–1811

  Article  Google Scholar 

• Saito K, Kurosawa M, Tatsuguchi K, Takagi Y, Murakoshi I (1994) Modulation of cysteine biosynthesis in chloroplasts of transgenic tobacco overexpressing cysteine synthase [O-acetylserine(thiol)-lyase]. Plant Physiol 106:887–895

  Article  CAS  Google Scholar 

• Seelig GF, Meister A (1984) γ-glutamylcysteine synthetase: interactions of an essential sulfhydryl group. J Biol Chem 259:3534–3538

  CAS  Google Scholar 

• Sharma SS, Dietz KJ (2006) The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress. J Exp Bot 57:711–726

  Article  CAS  Google Scholar 

• Shri M, Kumar S, Chakrabarty D et al (2009) Effect of arsenic on growth, oxidative stress, and antioxidant system in rice seedlings. Ecotox Environ Safe 72:1102–1110

  Article  CAS  Google Scholar 

• Singh BK (ed) (1999) Biochemistry and biotechnology. Marcel Dekker, New York, p 621

  Google Scholar 

• Sok J, Calfon M, Lu J, Lichtlen P, Clark SG, Ron D (2001) Arsenite-inducible RNA-associated protein (AIRAP) protects from arsenite toxicity. Cell Stress Chap 6:6–15

  Article  CAS  Google Scholar 

• Srivastava S, Srivastava AK, Suprasanna P, D'Souza SF (2009) Comparative biochemical and transcriptional profiling of two contrasting varieties of Brassica juncea L. in response to arsenic exposure reveals mechanisms of stress perception and tolerance. J Exp Bot 60:3419–3431

  Article  CAS  Google Scholar 

• Srivastava S, Penna S, D'Souza SF (2010) Redox state and energetic equilibrium determine the magnitude of stress in Hydrilla verticillata upon exposure to arsenate. Protoplasma. doi:10.1007/s00709-010-0256-Z

• Taylor CB (1996) Proline and water deficit: ups, downs, ins and outs. Plant Cell 8:1222–1224

  Google Scholar 

• Thompson AR, Vierstra RD (2005) Autophagic recycling: lessons from yeast help to define the process in plants. Curr Opin Plant Biol 8:165–173

  Article  CAS  Google Scholar 

• Tripathi RD, Srivastava S, Mishra S, Singh N, Tuli R, Gupta DK, Maathuis FJM (2007) Arsenic hazards: strategies for tolerance and remediation by plants. Trends Biotech 25:158–165

  Article  CAS  Google Scholar 

• Tripathi P, Mishra A, Dwivedi S, Chakrabarty D, Trivedi PK, Singh RP, Tripathi RD (2012) Differential response of oxidative stress and thiol metabolism in contrasting rice genotypes for arsenic tolerance. Ecotox Environ Safe 79:189–198

  Article  CAS  Google Scholar 

• Tu C, Ma LQ (2005) Effect of arsenic on concentration and distribution of nutrients in the fronds of the arsenic hyperaccumulator Pteris vittata L. Environ Pollut 135:333–340

  Article  CAS  Google Scholar 

• Vasconcelos MT, Azenha M, De Freitas V (1999) Role of polyphenols in copper complexation in red wines. J Agric Food Chem 47:2791–2796

  Article  CAS  Google Scholar 

• Xiang C, Oliver DJ (1998) Glutathione metabolic genes coordinately respond to heavy metals and jasmonic acid in Arabidopsis. Plant Cell 10:1539–1550

  CAS  Google Scholar 

• Yamaji N, Mitatni N, Ma JF (2008) A transporter regulating silicon distribution in rice shoots. Plant Cell 20:1381–1389

  Article  CAS  Google Scholar 

• Zhao FJ, McGrath SP, Meharg AA (2010) Arsenic as a food chain contaminant: mechanisms of plant uptake and metabolism and mitigation strategies. Ann Rev Plant Biol 61:535–559

  Article  CAS  Google Scholar 

• Zoghlami LB, Djebali W, Abbes Z et al (2011) Metabolite modifications in Solanum lycopersicum roots and leaves under cadmium stress. Afr J Biotechnol 10:567–579

  CAS  Google Scholar 

Download references

The authors are thankful to Dr. C. S. Nautiyal, Director, CSIR - National Botanical Research Institute, Lucknow, for the facilities and for the financial support from the network projects (CSIR), New Delhi, India. Preeti Tripathi is thankful to the Council of Scientific and Industrial Research, New Delhi, India, for the award of Senior Research Fellowship.

1. CSIR - National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, - 226001, India

   Preeti Tripathi, Rudra Deo Tripathi, Sanjay Dwivedi, Debasis Chakrabarty & Prabodh K. Trivedi

2. Department of Environmental Sciences, Babasaheb Bhimrao Ambedkar (Central) University, Rae Bareily Road, Lucknow, - 226025, India

   Preeti Tripathi & Rana Pratap Singh

3. Rice Research Station, Chinsurah, Hooghly, Department of Agriculture, Government of West Bengal, - 712102, India

   Bijan Adhikari

1. Preeti Tripathi
2. Rudra Deo Tripathi
3. Rana Pratap Singh
4. Sanjay Dwivedi
5. Debasis Chakrabarty
6. Prabodh K. Trivedi
7. Bijan Adhikari

Correspondence to Rudra Deo Tripathi.

Responsible editor: Philippe Garrigues

Below is the link to the electronic supplementary material.

Tripathi, P., Tripathi, R.D., Singh, R.P. et al. Arsenite tolerance in rice (Oryza sativa L.) involves coordinated role of metabolic pathways of thiols and amino acids. Environ Sci Pollut Res 20, 884–896 (2013). https://doi.org/10.1007/s11356-012-1205-5

Download citation

• Received: 16 February 2012

• Accepted: 17 September 2012

• Published: 04 October 2012

• Issue Date: February 2013

• DOI: https://doi.org/10.1007/s11356-012-1205-5

RetroSearch is an open source project built by @garambo | Open a GitHub Issue

Search and Browse the WWW like it's 1997 | Search results from DuckDuckGo

HTML: 3.2 | Encoding: UTF-8 | Version: 0.7.4