The effect of chromium (Cr) on photosystem II (PSII) electron transport and the change of proteins content within PSII complex were investigated. When Lemna gibba was exposed to Cr during 96 h, growth inhibition was found to be associated with an alteration of the PSII electron transport at both PSII oxidizing and reducing sides. Investigation of fluorescence yields at transients K, J, I, and P suggested for Cr inhibitory effect to be located at the oxygen-evolving complex and QA reduction. Those Cr-inhibitory effects were related to the change of the turnover of PSII D1 protein and the alteration of 24 and 33 kDa proteins of the oxygen-evolving complex. The inhibition of the PSII electron transport and the formation of reactive oxygen species induced by Cr were highly correlated with the decrease in the content of D1 protein and the amount of 24 and 33 kDa proteins. Therefore, functional alteration of PSII activity by Cr was closely related with the structural change within PSII complex.
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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. AbbreviationsAntenna size of an active reaction center of photosystem II
Chromium
A core protein of the photosystem II reaction center
2,6–Dichlorophenol indophenol
Non-photochemical energy dissipation of an active PSII reaction center
Electron transport in an active reaction center of photosystem II
Fluorescence yield at 50 µs when all reaction centers of PSII are open
Maximal fluorescence yield at P fluorescence transient; LHCII: light harvesting complex of photosystem II
Oxygen-evolving complex
Photosystem II
Quinone A as the primary electron carrier within PSII
Quinone B as the secondary electron carrier within PSII
The amount of active PSII reaction centers per excited cross section
reactive oxygen species
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This research was supported by the Natural Science and Engineering Council of Canada (NSERC) through Grant GP0093404 awarded to R. Popovic. Special acknowledgment goes to Dr. Matilde Baron from the Department of Biochemistry (EEZ, CSIC, Granada, Spain) for kindly supplying the primary antibodies used in the present study for immuno-detection of PSII D1, LHCII and OEC polypeptides.
Author information Authors and AffiliationsDepartment of Chemistry, Environmental Toxicology Research Center, University of Quebec in Montreal, TOXEN, 2101, Jeanne-Mance, H2X 2J6, Montreal, Quebec, Canada
Nadia Ait Ali, David Dewez, Olivier Didur & Radovan Popovic
Correspondence to Radovan Popovic.
About this article Cite this articleAli, N.A., Dewez, D., Didur, O. et al. Inhibition of photosystem II photochemistry by Cr is caused by the alteration of both D1 protein and oxygen evolving complex. Photosynth Res 89, 81–87 (2006). https://doi.org/10.1007/s11120-006-9085-5
Received: 02 March 2006
Accepted: 10 July 2006
Published: 13 September 2006
Issue Date: September 2006
DOI: https://doi.org/10.1007/s11120-006-9085-5
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