Home - News ( United States | United Kingdom | Italy | Germany ) - Football scores

Showing content from https://link.springer.com/doi/10.1007/s11103-005-5102-x below:

Identification of differentially expressed genes in leaf and root between wheat hybrid and its parental inbreds using PCR-based cDNA subtraction

Heterosis was defined as the advantage of hybrid performance over its parents in terms of growth and productivity. Previous studies showed that differential gene expression between hybrids and their parents is responsible for the heterosis; however, information on systematic identification and characterization of the differentially expressed genes are limited. In this study, an interspecific hybrid between common wheat (Triticum aestivum. L., 2n = 6x = 42, AABBDD) line 3338 and spelt (Triticum spelta L. 2n = 6x = 42, AABBDD) line 2463 was found to be highly heterotic in both aerial growth and root related traits, and was then used for expression assay. A modified suppression subtractive hybridization (SSH) was used to generate four subtracted cDNA libraries, and 748 nonreduandant cDNAs were obtained, among which 465 had high sequence similarity to the GenBank entries and represent diverse of functional categories, such as metabolism, cell growth and maintenance, signal transduction, photosynthesis, response to stress, transcription regulation and others. The expression patterns of 68.2% SSH-derived cDNAs were confirmed by reverse Northern blot, and semi-quantitative RT-PCR exhibited the similar results (72.2%). And it was concluded that the genes differentially expressed between hybrids and their parents involved in diverse physiological process pathway, which might be responsible for the observed heterosis.

This is a preview of subscription content, log in via an institution to check access.

Springer+ Basic

€34.99 /Month

• Get 10 units per month
• Download Article/Chapter or eBook
• 1 Unit = 1 Article or 1 Chapter
• Cancel anytime

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

BPH:

best-parent heterosis

GO:

gene ontology

LRL:

longest root length

MPH:

mid-parent heterosis

RAD:

root average diameter

RDW:

root dry weight

RSA:

root surface area

RSR:

root/shoot ratio

RTN:

root tips number

RT-PCR:

reverse transcription polymerase chain reaction

RV:

root volume

SSH:

suppression subtractive hybridization

TL:

taproot length

TN:

taproot number

TRL:

total root length

• R. Aeschbacher K.W. Schiefelbein P.N. Benfey (1994) ArticleTitleThe genetic and molecular basis of root development Ann. Rev. Plant Physio. Plant Mol. Biol. 45 25–45

  Google Scholar 

• R.W. Allard P. Garcia L.E. Saenz-de-Miera M. Perez la Vega Particlede (1993) ArticleTitleEvolution of multilocus genetic structure in Avena hirtula and Avena barbata Genetics 135 1125–1139

  Google Scholar 

• Altschul F. Stephen L. Thomas Madden Alejandro A. Schaffer J. Zhang Z. Zhang W. Miller D.J. Lipman (1997) ArticleTitleGapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucl. Acids Res. 25 3389–3402

  Google Scholar 

• J.A. Birchler D.L. Auger N.C. Riddle (2003) ArticleTitleIn search of the molecular basis of heterosis Plant Cell 15 2236–2239

  Google Scholar 

• W. Broughton M. Dilworth (1971) ArticleTitleControl of leghaemoglobin synthesis in snake beans Biochem. J. 125 1075–1080

  Google Scholar 

• L. Comai (2000a) ArticleTitleGenetic and epigenetic interactions in allopolyploid plants Plant Mol. Biol. 43 387–399

  Google Scholar 

• L. Comai A.P. Tyagi K. Winter R. Holmes-Davis S. Reynolds Y. Stevens B. Byers (2000b) ArticleTitlePhenotypic instability and rapid gene silencing in newly formed Arabidopsis allotetraploids Plant Cell 12 1551–1568

  Google Scholar 

• J. Doebley L. Lukens (1998) ArticleTitleTranscriptional regulators and the evolution of plant form Plant Cell 10 1075–1082

  Google Scholar 

• D.N. Duvick (1997) Heterosis: feeding people and protecting natural resources J.G. Coors S. Pandey (Eds) Genetics and Exploitation of Heterosis in Crops American Society of Agronomy, Inc Madison, WI, USA 19–29

  Google Scholar 

• T.A. Edwards J. Trincao C.R. Escalante R.P. Wharton A.K. Aggarwal (2000) ArticleTitleCrystallization and characterization of pumilio: a novel RNA binding protein J. Struct. Biol. 132 251–254

  Google Scholar 

• M. Frahm E. Foster J. Kelly (2003) ArticleTitleIndirect screening techniques for drought resistance in dry bean Annu. Rep. Bean Improv. Coop. 46 87–88

  Google Scholar 

• T. Galitski A.J. Saldanha C.A. Styles E.S. Lander G.R. Fink (1999) ArticleTitlePloidy regulation of gene expression Science 285 251–254

  Google Scholar 

• J. Gao S. Kim Y. Chung J. Lee (1994) ArticleTitleDevelopmental and environmental regulation of two ribosomal protein genes in tobacco Plant Mol. Biol. 25 761–770

  Google Scholar 

• F. Gonzalez A. Delahodde T. Kodadek S.A. Johnston (2002) ArticleTitleRecruitment of a 19S proteasome subcomplex to an activated promoter Science 296 548–550

  Google Scholar 

• M. Guo D. Davis J.A. Birchler (1996) ArticleTitleDosage effects on gene expression in a maize ploidy series Genetics 142 1349–1355

  Google Scholar 

• G. Harris G. Campbell (1989) ArticleTitleAutomated quantification of roots using a simple image analyzer Agron. J. 81 935–938

  Google Scholar 

• J.P. Hua Y.Z. Xing W.R. Wu C.G. Xu X.L. Sun S.B. Yu Q.F. Zhang (2003) ArticleTitleSingle-locus heterotic effects and dominance by dominance interactions can adequately explain the genetic basis of heterosis in an elite rice hybrid Proc. Natl. Acad. Sci. USA 100 2574–2579

  Google Scholar 

• In-cheol Jang Yoon-Mok Pahk Sang lk Song Ho JEONG Kwon Baek Hie Nahm Ju-Kon Kim (2003) ArticleTitleStructure and expression of the rice class-I type histone deacetylase genes OsHDAC1-3: OsHDAC1 overexpression in transgenic plants leads to increased growth rate and altered architecture Plant J. 33 531–541

  Google Scholar 

• T. Ito G.T. Kim K. Shinozaki (2000) ArticleTitleDisruption of an Arabidopsis S-13 homologous gene by transposon-mediated mutagenesis causes aberrant growth and development Plant J. 22 257–264

  Google Scholar 

• C. Jiang R.J. Wright K.M. El-Zik A.H. Paterson (1998) ArticleTitlePolyploid formation created unique avenues for response to selection in Gossypium Proc. Natl. Acad. Sci. USA 95 4419–4424

  Google Scholar 

• K.M. Kashkush Feldman A. Levy (2002) ArticleTitleGene loss, silencing and activation in a newly synthesized wheat allotetraploid Genetics 160 1651–1659

  Google Scholar 

• K.M. Kashkush Feldman A. Levy (2003) ArticleTitleTranscriptional activation of retrotransposons alters the expression of adjacent genes in wheat Nat. Genet. 33 102–106

  Google Scholar 

• R.D. Kornberg Y. Lorch (1999) ArticleTitleTwenty-five years of the nucleosome: fundamental particle of the eukaryote chromosome Cell 98 285–294

  Google Scholar 

• J. Masterson (1994) ArticleTitleStomatal size in fossil plants: evidence for polyploidy in majority of angiosperms Science 264 421–423

  Google Scholar 

• R.G. McDaniel (1985) ArticleTitleBiochemical and physiological basis of heterosis CRC Crit. Rev. Plant Sci. 4 227–246

  Google Scholar 

• R.C. Meyer OttÓ Törjēk M. becher T. Altmann (2004) ArticleTitleHeterosis of biomass production in Arabidopsis Establishment during early development Plant Physiol. 134 1813–1823

  Google Scholar 

• O. Mittelsten Scheid L. Jakovleva K. Afsar J. Maluszynska J. Paszkowski (1996) ArticleTitleA change of ploidy can modify epigenetic silencing Proc. Natl. Acad. Sci. USA 93 7114–7119

  Google Scholar 

• Z.F. Ni Q.X. Sun Z.Y. Liu (2000) ArticleTitleIdentification of a hybrid-specific expressed gene encoding novel RNA-binding protein in wheat seedling leaves using differential display of mRNA Mol. Gen. Genet. 263 934–938

  Google Scholar 

• Z.F. Ni Q.X. Sun L.M. Wu C.J. Xie (2002) ArticleTitleDifferential gene expression between wheat hybrids and their parental inbreds in primary roots Acta Bot. Sin. 44 457–462

  Google Scholar 

• T.C. Osborn J.C. Pires J.A. Birchler D.L. Auger Z.J. Chen H.S. Lee L. Comai A. Madlung R.W. Doerge V. Colot R.A. Martienssen (2003) ArticleTitleUnderstanding mechanisms of novel gene expression in polyploids Trends Genet. 19 141–147

  Google Scholar 

• Pickett A. 1993. Hybrid Wheat – Results and Problems. Adv. Plant Breeding, Parey, Berlin, 15)

• E. Robbins T.W. Borun (1967) ArticleTitleThe cytoplasmic synthesis of histones in Hela cells and its temporal relationship to DNA replication Proc. Natl. Acad. Sci. USA 57 409–416

  Google Scholar 

• S. Romagnoli M. Maddaloni C. Livini M. Motto (1990) ArticleTitleRelationship between gene expression and hybrid vigor in primary root tips of young maize (Zea mays L.) plantlets Theor. Appl. Genet. 80 769–775

  Google Scholar 

• C.W. Stuber S.E. Lincoln D.W. Wolff T. Helentjaris E.S. Lander (1992) ArticleTitleIdentification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers Genetics 132 823–839

  Google Scholar 

• Q.X. Sun Z.F. Ni Z.Y. Liu (1999) ArticleTitleDifferential gene expression between wheat hybrids and their parental inbreds in seedling leaves Euphytica 106 117–123

  Google Scholar 

• Q.X. Sun L.M. Wu Z.F. Ni F.R. Meng Z.K. Wang Z. Lin (2004) ArticleTitleDifferential gene expression patterns in leaves between hybrids and their parental inbreds are correlated␣with heterosis in a wheat diallel cross Plant Sci. 166 651–657

  Google Scholar 

• K.J.P.T. Heuvel ParticleVan den R.J. G.W.M. Esch Barendse ParticleVan G.J Wullems (1999) ArticleTitleIsolation and molecular characterization of gibberellin-regulated H1 and H2B histone cDNAs in the leaf of the gibberellin-deficient tomato Plant Mol. Biol. 39 883–890

  Google Scholar 

• D. Weijers Franke-van M. Dijk R-J. Venken A, Quint (2001) ArticleTitleAn Arabidopsis minute-like phenotype caused by a semi-dominant mutation in a ribosomal protein S5 Genes Dev. 128 4289–4299

  Google Scholar 

• J. Wendel (2000) ArticleTitleGenome evolution in polyploids Plant Mol. Biol. 42 225–249

  Google Scholar 

• L.M. Wu Z.F. Ni F.R. Meng Z. Lin Q.X. Sun (2003) ArticleTitleCloning and characterization of leaf cDNAs that are differentially expressed between wheat hybrids and their parents Mol. Gen. Genet. 270 281–286

  Google Scholar 

• J. Xiao J. Li L. Yuan S.D. Tanksley (1995) ArticleTitleDominance is the major genetic basis of heterosis in rice as revealed by QTL analysis using molecular markers Genetics 140 745–754

  Google Scholar 

• L.Z. Xiong G.P. Yang C.G. Xu Q.F. Zhang M.A. Saghai Maroof (1998) ArticleTitleRelationships of differential gene expression in leaves with heterosis and heterozygosity in a rice diallel cross Mol. Breed. 4 129–136

  Google Scholar 

• M. Yabba E. Foster (2003) ArticleTitleCommon bean root response to abscisic acid treatment Annu. Rep. Bean Improv. Coop. 46 85–86

  Google Scholar 

• S. Yu J.X. Li C.G. Xu Y.F. Tan Y.J. Gao X.H. Li Q.F. Zhang M.A.S. Maroof (1997) ArticleTitleImportance of epistasis as the genetic basis of heterosis in an elite rice hybrid Proc. Natl. Acad. Sci. USA 94 9226–9231

  Google Scholar 

• J. Zhao B.K. Kennedy B.D. Lawrence D.A. Barbie A.G. Matera J.A. Fletcher E. Harlow (2000) ArticleTitleNPAT links cyclin E-Cdk2 to the regulation of replication-dependent histone gene transcription Genes Dev. 14 2283–2297

  Google Scholar 

Download references

1. Department of Plant Genetics & Breeding and State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, 100094, Beijing, China

   Yingyin Yao, Zhongfu Ni, Yinhong Zhang, Yan Chen, Yuhua Ding, Zongfu Han, Zhiyong Liu & Qixin Sun

2. Department of Plant Genetics & Breeding, China Agricultural University, Yuanmingyuan Xi Road no. 2, 100094, Beijing, Haidian District, China

   Qixin Sun

1. Yingyin Yao
2. Zhongfu Ni
3. Yinhong Zhang
4. Yan Chen
5. Yuhua Ding
6. Zongfu Han
7. Zhiyong Liu
8. Qixin Sun

Correspondence to Qixin Sun.

These authors contribute equally to this work

Yao, Y., Ni, Z., Zhang, Y. et al. Identification of differentially expressed genes in leaf and root between wheat hybrid and its parental inbreds using PCR-based cDNA subtraction. Plant Mol Biol 58, 367–384 (2005). https://doi.org/10.1007/s11103-005-5102-x

Download citation

• Received: 10 December 2004

• Accepted: 05 April 2005

• Issue Date: June 2005

• DOI: https://doi.org/10.1007/s11103-005-5102-x

RetroSearch is an open source project built by @garambo | Open a GitHub Issue

Search and Browse the WWW like it's 1997 | Search results from DuckDuckGo

HTML: 3.2 | Encoding: UTF-8 | Version: 0.7.4