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Cultivation conditions and properties of extracellular crude lipase from the psychrotrophic fungus Penicillium chrysogenum 9′

Abstract

Among 97 fungal strains isolated from soil collected in the arctic tundra (Spitsbergen), Penicillium chrysogenum 9′ was found to be the best lipase producer. The maximum lipase activity was 68 units mL−1 culture medium on the fifth day of incubation at pH 6.0 and 20°C. Therefore, P. chrysogenum 9′ was classified as a psychrotrophic microorganism. The non-specific extracellular lipase showed a maximum activity at 30°C and pH 5.0 for natural oils or at pH 7.0 for synthetic substrates. Tributyrin was found to be the best substrate for lipase, among those tested. The Km and Vmax were calculated to be 2.33 mM and 22.1 units mL−1, respectively, with tributyrin as substrate. The enzyme was inhibited more by EDTA than by phenylmethylsulfonyl fluoride and was reactivated by Ca2+. The P. chrysogenum 9′ lipase was very stable in the presence of hexane and 1,4-dioxane at a concentration of 50%, whereas it was unstable in presence of xylene.

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Acknowledgement

This work was supported by the Polish Science Committee BS/BiNoZ/4.

Author information Authors and Affiliations

  1. Department of Biochemistry, Maria Curie-Sklodovska University, Sklodovska Square 3, Lublin, 20-031, Poland

    R. Bancerz

  2. Department of Biochemistry, Skubiszewski Medical University, 1 Chodźki St., Lublin, 20-093, Poland

    G. Ginalska

  3. Department of Industrial Microbiology, Maria Curie-Sklodovska University, 19 Akademicka St., Lublin, 20-033, Poland

    J. Fiedurek & A. Gromada

Authors
  1. R. Bancerz
  2. G. Ginalska
  3. J. Fiedurek
  4. A. Gromada
Corresponding author

Correspondence to R. Bancerz.

About this article Cite this article

Bancerz, R., Ginalska, G., Fiedurek, J. et al. Cultivation conditions and properties of extracellular crude lipase from the psychrotrophic fungus Penicillium chrysogenum 9′. J IND MICROBIOL BIOTECHNOL 32, 253–260 (2005). https://doi.org/10.1007/s10295-005-0235-0

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