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Two sub-states of the red2 state of methyl-coenzyme M reductase revealed by high-field EPR spectroscopy

Methyl-coenzyme M reductase (MCR) catalyzes the formation of methane from methyl-coenzyme M and coenzyme B in methanogenic archaea. The enzyme has two structurally interlinked active sites embedded in an α₂β₂γ₂ subunit structure. Each active site has the nickel porphyrinoid F₄₃₀ as a prosthetic group. In the active state, F₄₃₀ contains the transition metal in the Ni(I) oxidation state. The active enzyme exhibits an axial Ni(I)-based continuous wave (CW) electron paramagnetic resonance (EPR) signal, called red1a in the absence of substrates or red1c in the presence of coenzyme M. Addition of coenzyme B to the MCR-red1 state can partially and reversibly convert it into the MCR-red2 form, which shows a rhombic Ni(I)-based EPR signal (at X-band microwave frequencies of approximately 9.4 GHz). In this report we present evidence from high-field/high-frequency CW EPR spectroscopy (W-band, microwave frequency of approximately 94 GHz) that the red2 state consists of two substates that could not be resolved by EPR spectroscopy at X-band frequencies. At W-band it becomes apparent that upon addition of coenzyme B to MCR in the red1c state, two red2 EPR signals are induced, not one as was previously believed. The first signal is the well-characterized (ortho)rhombic EPR signal, thus far called red2, while the second previously unidentified signal is axial. We have named the two substates MCR-red2r and MCR-red2a after their rhombic and axial signals, respectively.

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CH₃-S-CoM:

Methyl-coenzyme M

CW:

Continuous wave

EPR:

Electron paramagnetic resonance

HS-CoB:

Coenzyme B

HS-CoM:

Coenzyme M

HYSCORE:

Hyperfine sublevel correlation

MCR:

Methyl-coenzyme M reductase

1. Thauer RK (1998) Microbiology 144:2377–2406

   Article  PubMed  CAS  Google Scholar 

2. Thauer RK, Jungermann K, Decker K (1977) Bacteriol Rev 41:100–180

   PubMed  CAS  Google Scholar 

3. Ermler U, Grabarse W, Shima S, Goubeaud M, Thauer RK (1997) Science 278:1457–1462

   Article  PubMed  CAS  Google Scholar 

4. Goubeaud M, Schreiner G, Thauer RK (1997) Eur J Biochem 243:110–114

   Article  PubMed  CAS  Google Scholar 

5. Pelmenschikov V, Blomberg MRA, Siegbahn PEM, Crabtree RH (2002) J Am Chem Soc 124:4039

   Article  PubMed  CAS  Google Scholar 

6. Kunz RC, Horng YC, Ragsdale SW (2006) J Biol Chem 281:34663–34676

   Article  PubMed  CAS  Google Scholar 

7. Mahlert F, Grabarse W, Kahnt J, Thauer RK, Duin EC (2002) J Biol Inorg Chem 7:101–112

   Article  PubMed  CAS  Google Scholar 

8. Goenrich M, Duin EC, Mahlert F, Thauer RK (2005) J Biol Inorg Chem 10:333–342

   Article  PubMed  CAS  Google Scholar 

9. Wasserfallen A, Nolling J, Pfister P, Reeve J, Conway de Macario E (2000) Int J Syst Evol Microbiol 50(Pt 1):43–53

   PubMed  CAS  Google Scholar 

10. Gunsalus RP, Romesser JA, Wolfe RS (1978) Biochemistry 17:2374–2377

    Article  PubMed  CAS  Google Scholar 

11. Kobelt A, Pfaltz A, Ankel-Fuchs D, Thauer RK (1987) FEBS Lett 214:265–268

    Article  CAS  Google Scholar 

12. Ellermann J, Hedderich R, Böcher R, Thauer RK (1988) Eur J Biochem 172:669–677

    Article  PubMed  CAS  Google Scholar 

13. Rospert S, Linder D, Ellermann J, Thauer RK (1990) Eur J Biochem 194:871–877

    Article  PubMed  CAS  Google Scholar 

14. Bonacker LG, Baudner S, Mörschel E, Böcher R, Thauer RK (1993) Eur J Biochem 217:587–595

    Article  PubMed  CAS  Google Scholar 

15. Duin EC, Signor L, Piskorski R, Mahlert F, Clay MD, Goenrich M, Thauer RK, Jaun B, Johnson MK (2004) J Biol Inorg Chem 9:563–576

    Article  PubMed  CAS  Google Scholar 

16. Mahlert F, Bauer C, Jaun B, Thauer RK, Duin EC (2002) J Biol Inorg Chem 7:500–513

    Article  PubMed  CAS  Google Scholar 

17. Bradford MM (1976) Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

18. Stoll S, Schweiger A (2006) J Magn Reson 178:42

    Article  PubMed  CAS  Google Scholar 

19. Finazzo C, Harmer J, Bauer C, Jaun B, Duin EC, Mahlert F, Goenrich M, Thauer RK, Van Doorslaer S, Schweiger A (2003) J Am Chem Soc 125:4988–4989

    Article  PubMed  CAS  Google Scholar 

20. Finazzo C, Harmer J, Jaun B, Duin EC, Mahlert F, Thauer RK, Van Doorslaer S, Schweiger A (2003) J Biol Inorg Chem 8:586–593

    PubMed  CAS  Google Scholar 

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We thank the Swiss National Science Foundation (SNF) and the Fonds der Chemischen Industrie for financial support. D.H. gratefully acknowledges a research scholarship (HI 1094/1-1) from the Deutsche Forschungsgemeinschaft (DFG).

1. Laboratorium für Physikalische Chemie, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093, Zurich, Switzerland

   Denise I. Kern, Jeffrey Harmer & Dariush Hinderberger

2. Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128, Mainz, Germany

   Dariush Hinderberger

3. Laboratorium für Organische Chemie, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093, Zurich, Switzerland

   Bernhard Jaun

4. Max-Planck-Institut für Terrestrische Mikrobiologie, Karl-von-Frisch-Straße, 35043, Marburg, Germany

   Meike Goenrich & Rudolf K. Thauer

1. Denise I. Kern
2. Meike Goenrich
3. Bernhard Jaun
4. Rudolf K. Thauer
5. Jeffrey Harmer
6. Dariush Hinderberger

Correspondence to Dariush Hinderberger.

Below is the link to the electronic supplementary material.

Kern, D.I., Goenrich, M., Jaun, B. et al. Two sub-states of the red2 state of methyl-coenzyme M reductase revealed by high-field EPR spectroscopy. J Biol Inorg Chem 12, 1097–1105 (2007). https://doi.org/10.1007/s00775-007-0281-3

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• Received: 18 May 2007

• Accepted: 18 July 2007

• Published: 10 August 2007

• Issue Date: November 2007

• DOI: https://doi.org/10.1007/s00775-007-0281-3

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