Recombination between direct repeats has been studied in Penicillium chrysogenum using strain TD7-88 (lys− pyr+), which contains two inactive copies of the lys2 gene separated by 4.5 kb of DNA (including the pyrG gene) in its genome. Gene conversion leading to products with the lys+ pyr+ phenotype was observed at a frequency of 1 in 3.2 × 103 viable spores. Two types of deletion events giving rise to lys+ pyr− and lys− pyr− phenotypes were obtained with different frequencies. Southern analysis revealed that gene conversion occurs mainly as a result of crossing over events that remove the BamHI frameshift mutation present in one of the repeats. In lys− pyr− recombinants, the deletion events do not affect the frameshift mutation in the BamHI site, while lys+ pyr− recombinants showed repair of the BamHI frameshift mutation and the genotype of the parental non-disrupted strain was restored. In summary, deletion events in P. chrysogenum tend to favor the restoration of the phenotype and genotype characteristic of the parental non-disrupted strain.
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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. Author information Authors and AffiliationsInstitute of Biotechnology (INBIOTEC), Science Park of León, Avda del Real 1, E-24006 León, Spain, , , , , , ES
J. Casqueiro, S. Gutiérrez, M. J. Hijarrubia & J. F. Martín
Area of Microbiology, Faculty of Biology, University of León, E-24071 León, Spain e-mail: degjmm@unileon.es; Fax: + 34-987-291506, , , , , , ES
O. Bañuelos
Received: 9 November 1998 / Accepted: 14 April 1999
About this article Cite this articleCasqueiro, J., Bañuelos, O., Gutiérrez, S. et al. Intrachromosomal recombination between direct repeats in Penicillium chrysogenum: gene conversion and deletion events. Mol Gen Genet 261, 994–1000 (1999). https://doi.org/10.1007/s004380051048
Issue Date: August 1999
DOI: https://doi.org/10.1007/s004380051048
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