In arbuscular mycorrhizas, H+-ATPase is active in the plant membrane around arbuscules but absent from plant mutants defective in arbuscule development (Gianinazzi-Pearson et al. 1995, Can J Bot 73: S526–S532). The proton-pumping H+-ATPase is encoded by a family of genes in plants. Immunocytochemical studies and promoter-gusA fusion assays were performed in transgenic tobacco (Nicotiana tabacum L.) to determine whether the periarbuscular enzyme activity results from de-novo activation of plant genes by an arbuscular mycorrhizal fungus. The H+-ATPase protein was localized in the plant membrane around arbuscule hyphae. The enzyme was absent from non-colonized cortical cells. Regulation of seven H+-ATPase genes (pma) was compared in non-mycorrhizal and mycorrhizal roots by histochemical detection of β-glucuronidase (GUS) activity. Two genes (pma2, pma4) were induced in arbuscule-containing cells of mycorrhizal roots but not in non-mycorrhizal cortical tissues or senescent mycorrhiza. It is concluded that de-novo H+-ATPase activity in the periarbuscular membrane results from selective induction of two H+-ATPase genes, which can have diverse roles in plant-fungal interactions at the symbiotic interface.
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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. Author information Authors and AffiliationsLaboratoire de Phytoparasitologie INRA/CNRS, Centre de Microbiologie du Sol et de l'Environnement-Institut National de la Recherche Agronomique, BV 1540, 21034 Dijon cedex, France, , , , , , FR
V. Gianinazzi-Pearson, C. Arnould & S. Gianinazzi
Unité de Biochimie Physiologique, Université Catholique de Louvain, Place Croix du Sud, 2-20, 1348 Louvain-la-Neuve, Belgium, , , , , , BE
M. Oufattole & M. Arango
Received: 23 October 1999 / Accepted: 7 February 2000
About this article Cite this articleGianinazzi-Pearson, V., Arnould, C., Oufattole, M. et al. Differential activation of H+-ATPase genes by an arbuscular mycorrhizal fungus in root cells of transgenic tobacco. Planta 211, 609–613 (2000). https://doi.org/10.1007/s004250000323
Issue Date: October 2000
DOI: https://doi.org/10.1007/s004250000323
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