PCR primers closely flanking the repeat region were redesigned to reduce the amplicon length of the selected STRs down to approximately 100 bp for the shorter alleles (loci HumTH01, D10S2325, DYS19 and DYS391). Highly degraded DNA (e.g. formalin-fixed tissue) and very low amounts of DNA could be more successfully typed using the new redesigned primers compared to the established sequences generating longer amplicons.
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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. Author information Authors and AffiliationsInstitute of Pathology and Legal Medicine, Department of Legal Medicine, University of Ulm, Prittwitzstrasse 6, 89075 Ulm, Germany, , , , , , DE
P. Wiegand
Institute of Legal Medicine, Martin-Luther-University Halle-Wittenberg, Franzosenweg 1, 06112 Halle, Germany, , , , , , DE
M. Kleiber
Received: 6 January 2000 / Accepted: 26 April 2000
About this article Cite this articleWiegand, P., Kleiber, M. Less is more – length reduction of STR amplicons using redesigned primers. Int J Leg Med 114, 285–287 (2001). https://doi.org/10.1007/s004140000162
Issue Date: April 2001
DOI: https://doi.org/10.1007/s004140000162
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