We developed a short tandem repeat (STR) typing kit based on DNA database systems that are included in, for example, the Interpol Standard Set of Loci recommendations (i.e., TH01, VWA, D3S1358, FGA) and the gender typing system Amelogenin. Two different multiplex sets were tested using the fluorescent dyes FAM, JOE, and VIC. The PCR results were compared to the commercially available AmpFISTR Blue kit, which contains the STRs D3S1358, VWA, and FGA. The advantage of our multiplex compared with the Blue kit was the generation of shorter amplicons (<200 bp) and the higher combined power of discrimination.
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Department of Legal Medicine, University Hospital, Prittwitzstr. 6, 89075, Ulm, Germany
P. Wiegand & R. Klein
genFOR GmbH, Altenahrer Strasse 9, 53501, Grafschaft-Gelsdorf, Germany
G. Braunschweiger
Institute of Legal Medicine, Röntgenstr. 23, 48149, Münster, Germany
C. Hohoff & B. Brinkmann
Correspondence to P. Wiegand.
About this article Cite this articleWiegand, P., Klein, R., Braunschweiger, G. et al. Short amplicon STR multiplex for stain typing. Int J Legal Med 120, 160–164 (2006). https://doi.org/10.1007/s00414-005-0048-8
Received: 09 May 2005
Accepted: 07 September 2005
Published: 08 November 2005
Issue Date: May 2006
DOI: https://doi.org/10.1007/s00414-005-0048-8
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