Validation studies were carried out using the commercially available PCR multiplex system genRESMPX-2. In addition to amelogenin, this system comprises the complete set of eight STR systems which are components of the German DNA database established in 1998 by the Federal Criminal Office of Germany (BKA). The minimum amount of template DNA which gave a complete DNA pattern ranged between 100 pg and 200 pg. Mixed samples could clearly be assigned from ratios between 1:5 (ACTBP2) and 1:20 (VWA, FGA). Experimental investigations with different forensic materials, environmental studies, reproducibility and precision data as well as practical casework analysis revealed that the genRESMPX-2 kit can be regarded as a sensitive, reliable and robust multiplex system even in the case of samples containing limited amounts or degraded DNA.
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The authors wish to acknowledge Marianne Steevens and Waltraud Hargesheimer for helpful technical assistance.
Author information Authors and AffiliationsInstitute of Legal Medicine, Rheinische Friedrich-Wilhelms-University Bonn, Stiftsplatz 12, 53111 , Bonn, Germany
A. Junge & B. Madea
Institute for Forensic Medicine, University Erlangen-Nürnberg, Universitätstrasse 22, 91054 , Erlangen, Germany
T. Lederer
genFOR GmbH, Altenahrer Strasse 9, 53501 , Grafschaft, Germany
G. Braunschweiger
Correspondence to A. Junge.
Additional informationA. Junge and T. Lederer contributed equally to the data presented
About this article Cite this articleJunge, A., Lederer, T., Braunschweiger, G. et al. Validation of the multiplex kit genRESMPX-2 for forensic casework analysis. Int J Legal Med 117, 317–325 (2003). https://doi.org/10.1007/s00414-003-0385-4
Received: 16 January 2003
Accepted: 02 May 2003
Published: 10 October 2003
Issue Date: December 2003
DOI: https://doi.org/10.1007/s00414-003-0385-4
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