The effects of exogenous basic fibroblast growth factor (bFGF) on the repair of full-thickness cartilage defects were examined. Four-millimeter diameter, cylindrical defects were made in rabbit articular cartilage and were filled with human recombinant bFGF. The addition of bFGF to the defect induced the formation of a thick cartilage layer composed of chondrocytes and a metachromatic-stained matrix after 6 weeks. The score of the bFGF-treated tissue, as evaluated by a semiquantitative histological scale, was significantly higher than that of the untreated tissue. At 24 weeks, the cartilage-like matrix that contained the proteoglycans and type II collagen was thicker in the bFGF-treated tissue than in the untreated tissue. Immunohistochemical analysis of the tissues at 6–12 weeks with an anti-bFGF monoclonal antibody suggested that a single application of bFGF increased the number of differentiating chondrocytes that synthesized bFGF at a high level. In contrast, immunostaining of the tissues at 6–12 weeks with a monoclonal antibody against proliferating cell nuclear antigen showed that the number of proliferating cells in the bFGF-treated tissue was fewer than in the untreated tissue. These findings suggest that administration of bFGF into cartilagenous defects promotes the differentiation of chondrocytes and their matrix synthesis, and that this growth factor is useful for improving cartilage repair.
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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. Author information Authors and AffiliationsDepartment of Orthopedic Surgery, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, Hiroshima 734, Japan Tel.: +81-82-257-5233, Fax: +81-82-257-5234, , , , , , JP
E. Fujimoto, Y. Mochizuki, Y. Sumen & Y. Ikuta
Department of Orthopedic Surgery, Shimane Medical University, Izumo-shi, Shimane-ken, Japan, , , , , , JP
M. Ochi
Department of Biochemistry, Hiroshima University School of Dentistry, Hiroshima, Japan, , , , , , JP
Y. Kato
Received: 18 May 1998
About this article Cite this articleFujimoto, E., Ochi, M., Kato, Y. et al. Beneficial effect of basic fibroblast growth factor on the repair of full-thickness defects in rabbit articular cartilage. Arch Orth Traum Surg 119, 139–145 (1999). https://doi.org/10.1007/s004020050377
Issue Date: May 1999
DOI: https://doi.org/10.1007/s004020050377
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