Home - News ( United States | United Kingdom | Italy | Germany ) - Football scores

Showing content from https://link.springer.com/doi/10.1007/s00299-003-0698-2 below:

Chimeric RNA/DNA oligonucleotide-directed gene targeting in rice

Site-specific mutagenesis in a rice genome was obtained by introducing chimeric RNA/DNA oligonucleotides (COs) by means of particle bombardment. Three COs were designed to target the independent codons for Pro-171, Trp-548 and Ser-627 of the endogenous rice acetolactate synthase (ALS) gene so it would confer resistance to ALS-inhibiting herbicides. Sequencing of the ALS gene of herbicide-resistant plants demonstrated that the ALS sequence was modified in a site-specific fashion. The efficiency of gene conversion mediated by COs was estimated to be 1×10^-4. These results demonstrate that CO-directed gene targeting is feasible in rice.

This is a preview of subscription content, log in via an institution to check access.

Springer+ Basic

€34.99 /Month

• Get 10 units per month
• Download Article/Chapter or eBook
• 1 Unit = 1 Article or 1 Chapter
• Cancel anytime

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

ALS :

Acetolactate synthase

BS :

Bispyribac-sodium

Cf :

Chlorsulfuron

CO :

Chimeric RNA/DNA oligonucleotide

• Beetham PR, Kipp PB, Sawycky XL, Arntzen CJ, May GD (1999) A tool for functional plant genomics: chimeric RNA/DNA oligonucleotides cause in vivo gene-specific mutations. Proc Natl Acad Sci USA 96:8774–8778

  CAS  PubMed  Google Scholar 

• Chen L, Zhang S, Beachy RN, Fauquet CM (1998) A protocol for consistent, large-scale production of fertile transgenic rice plants. Plant Cell Rep 18:25–31

  Google Scholar 

• Chiu W, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J (1996) Engineered GFP as a vital reporter in plants. Cur Biol 6:325–330

  CAS  Google Scholar 

• Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18:659–668

  Google Scholar 

• Graham IR, Dickson G (2002) Gene repair and mutagenesis mediated by chimeric RNA-DNA oligonucleotides: chimeraplasty for gene therapy and conversion of single nucleotide polymorphisms (SNPs). Biochim Biophys Acta 1587:1-6

  Article  CAS  PubMed  Google Scholar 

• Hohn B, Puchta H (1999) Gene therapy in plants. Proc Natl Acad Sci USA 96:8321–8323

  Article  CAS  PubMed  Google Scholar 

• Kemper EL, Silva MJ, Arruda P (1996) Effect of microprojectile bombardment parameters and osmotic treatment on particle penetration and tissue damage in transiently transformed cultured immature maize (Zea mays L.) embryos. Plant Sci 121:85–93

  Article  Google Scholar 

• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

  CAS  Google Scholar 

• Nakagawa Y, Machida C, Machida Y, Toriyama K (2001) A system to induce the deletion of genomic sequences using R/RS site-specific recombination and the Ac transposon in transgenic rice plants. Theor Appl Genet 102:1136–1141

  Article  CAS  Google Scholar 

• Oh TJ, May GD (2001) Oligonucleotide-directed plant gene targeting. Cur Opin Biotechnol 12:169–172

  Article  CAS  Google Scholar 

• Shimizu T, Nakayama I, Nagayama K, Miyazawa T, Nezu Y (2002) Acetolactate synthase inhibitors. In: Boger P, Wakabayashi K, Hirai K (eds) Herbicide classes in development. Springer, Berlin Heidelberg New York, pp 1–41

• Toriyama K, Hinata K (1985) Cell suspension and protoplast culture in rice. Plant Sci 41:179–183

  Article  CAS  Google Scholar 

• Zhu T, Peterson DJ, Tagliani L, St. Clair G, Baszczynski C, Bowen B (1999) Targeted manipulation of maize genes in vivo using chimeric RNA/DNA oligonucleotides. Proc Natl Acad Sci USA 96:8768–8773

  PubMed  Google Scholar 

• Zhu T, Mettenburg K, Peterson DJ, Tagliani L, Baszczynski CL (2000) Engineering herbicide-resistant maize using chimeric RNA/DNA oligonucleotides. Nat Biotechnol 18:555–558

  CAS  PubMed  Google Scholar 

Download references

We are grateful to the Kumiai Chemical Industry Co, Shizuoka, Japan for the gifts of BS and valuable comments.

1. Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, 981-8555, Sendai, Japan

   A. Okuzaki & K. Toriyama

1. A. Okuzaki
2. K. Toriyama

Correspondence to K. Toriyama.

Communicated by H. Uchimiya

Okuzaki, A., Toriyama, K. Chimeric RNA/DNA oligonucleotide-directed gene targeting in rice. Plant Cell Rep 22, 509–512 (2004). https://doi.org/10.1007/s00299-003-0698-2

Download citation

• Received: 14 February 2003

• Revised: 22 July 2003

• Accepted: 23 July 2003

• Published: 21 November 2003

• Issue Date: February 2004

• DOI: https://doi.org/10.1007/s00299-003-0698-2

RetroSearch is an open source project built by @garambo | Open a GitHub Issue

Search and Browse the WWW like it's 1997 | Search results from DuckDuckGo

HTML: 3.2 | Encoding: UTF-8 | Version: 0.7.4