A fungus, Fusarium verticillioides (NRRL 26518), was isolated by screening soil samples using corn fiber xylan as carbon source. The extracellular xylanase from this fungal strain was purified to apparent homogeneity from the culture supernatant by ultrafiltration using a 30,000 cut-off membrane, octyl-Sepharose chromatography and Bio gel A-0.5 m gel filtration. The purified xylanase (specific activity 492 U/mg protein; MW 24,000; pI 8.6) displayed an optimum temperature at 50 °C and optimum pH at 5.5, a pH stability range from 4.0 to 9.5 and thermal stability up to 50 °C. It hydrolyzed a variety of xylan substrates mainly to xylobiose and higher short-chain xylooligosaccharides. No xylose was formed. The enzyme did not require metal ions for activity and stability.
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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. Author information Authors and AffiliationsFermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, Agricultural Research service, United States Department of Agriculture, Peoria, Illinois 61604, USA, , , , ,
B. Saha
Received revision: 2 April 2001
Electronic Publication
About this article Cite this articleSaha, .B. Xylanase from a newly isolated Fusarium verticillioides capable of utilizing corn fiber xylan. Appl Microbiol Biotechnol 56, 762–766 (2001). https://doi.org/10.1007/s002530100716
Received: 07 November 2000
Accepted: 20 April 2001
Issue Date: September 2001
DOI: https://doi.org/10.1007/s002530100716
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