Enterobacter cloacae NRRL B-23289 was isolated from local decaying wood/corn soil samples while screening for microorganisms for conversion of l-arabinose to fuel ethanol. The major product of fermentation by the bacterium was meso-2,3-butanediol (2,3-BD). In a typical fermentation, a BD yield of 0.4 g/g arabinose was obtained with a corresponding productivity of 0.63 g/l per hour at an initial arabinose concentration of 50 g/l. The effects of initial arabinose concentration, temperature, pH, agitation, various monosaccharides, and multiple sugar mixtures on 2,3-BD production were investigated. BD productivity, yield, and byproduct formation were influenced significantly within these parameters. The bacterium utilized sugars from acid plus enzyme saccharified corn fiber and produced BD (0.35 g/g available sugars). It also produced BD from dilute acid pretreated corn fiber by simultaneous saccharification and fermentation (0.34 g/g theoretical sugars).
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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. Author information Authors and AffiliationsFermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA e-mail: sahabc@mail.ncaur.usda.gov Tel.: +1-309-6816276 Fax: +1-309-6816686, , , , , , US
B. C. Saha & R. J. Bothast
Received: 17 December 1998 / Revision received: 9 March 1999 / Accepted: 20 March 1999
About this article Cite this articleSaha, B., Bothast, R. Production of 2,3-butanediol by newly isolated Enterobacter cloacae . Appl Microbiol Biotechnol 52, 321–326 (1999). https://doi.org/10.1007/s002530051526
Issue Date: September 1999
DOI: https://doi.org/10.1007/s002530051526
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