Two novel arabinofuranohydrolases (AXH-d3 and AXH-m23) were purified from Bifidobacterium adolescentis DSM 20083. Both enzymes were induced upon growth of Bi. adolescentis on xylose and arabinoxylan-derived oligosaccharides. They were only active with arabinoxylans and therefore denoted as arabinoxylan arabinofuranohydrolases. Their optimal activity was at pH 6 and 30–40 °C. They were very specific in their mode of action and were clearly different from AXH-m from Aspergillus awamori. AXH-m23 released only arabinosyl groups, which were linked to the C-2 or C-3 position of singly substituted xylose residues in arabinoxylan oligomers. AXH-d3 hydrolysed C-3-linked arabinofuranosyl residues of doubly substituted xylopyranosyl residues of arabinoxylans and arab- inoxylan-derived oligosaccharides. No activity was observed with C-2-linked arabinofuranosyl residues of these doubly substituted xylopyranosyl residues, or against C-2- and C-3-linked arabinofuranosyl residues of singly substituted xylopyranosyl residues. The combination of AXH-d3 and AXH-m showed low debranching activity with highly substituted glucurono-arabinoxylans. However, arabinoxylan from wheat flour was debranched almost completely.
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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. Author information Authors and AffiliationsDepartment of Food Technology and Nutritional Sciences, Food Science Group, Wageningen Agricultural University, PO Box 8129, 6700 EV Wageningen, The Netherlands Tel.: +31-317-481888 Fax: +31-317-484893, , , , , , NL
K. M. J. Van Laere, C. H. L. Voragen, T. Kroef, L. A. M. Van den Broek, G. Beldman & A. G. J. Voragen
Received: 12 January 1999 / Accepted: 17 January 1999
About this article Cite this articleVan Laere, K., Voragen, C., Kroef, T. et al. Purification and mode of action of two different arabinoxylan arabinofuranohydrolases from Bifidobacterium adolescentis DSM 20083. Appl Microbiol Biotechnol 51, 606–613 (1999). https://doi.org/10.1007/s002530051439
Issue Date: May 1999
DOI: https://doi.org/10.1007/s002530051439
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