Endo-mannanases and endo-xylanases cleave different heteromannans and xylans yielding mainly dimers and trimers of the corresponding sugars as end-products. However, in the early stages of hydrolysis, four purified mannanases and four xylanases from fungal and bacterial origin, examined in this study, showed a different pattern of released oligomers (determined up to the pentamers). Furthermore, some of these enzymes showed a preference for cleaving the polysaccharides in the middle of the chain while others acted more at the end. When the increase in the specific fluidity of mannan and xylan solutions per reducing sugar released (K v) was measured against the bleaching effect of the enzymes on softwood kraft pulp, a correlation was found. A xylanase from Penicillium simplicissimum (K v = 0.15 l mPa−1s−1g−1) and a mannanase from Sclerotium rolfsii (K v = 0.12 l mPa−1s−1g−1) applied in a O(QX)P bleaching sequence (O = oxygen delignification, X = treatment with hemicellulolytic enzymes, Q = chelation of metals, P = treatment with hydrogen peroxide in alkaline solution) gave a high brightness increase of 3.0% and 1.9% ISO respectively. A less significant brightness increase was obtained with enzymes showing lower K v values, such as a xylanase from Schizophyllum commune (Kv = 0.051 l mPa−1s−1g−1, 0.2% ISO) and a bacterial mannanase (K v = 0.061 l mPa−1s−1g−1,0.5% ISO).
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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. Author information Authors and AffiliationsInstitute of Biotechnology, SFB Biokatalyse, Graz University of Technology Petersgasse 12, A-8010 Graz, Austria Tel.: (+43) 316/873/8408 Fax: (+43) 316/873/8434 e-mail: guebitz@biote.tu-graz.ac.at, , , , , , AT
G. M. Gübitz, B. Latal & W. Steiner
Institute of Food Technology, University of Agriculture, Vienna Muthgasse 18, A-1190 Wien, Austria, , , , , , AT
D. Haltrich
Received: 19 December 1996 / Received revision: 20 February 1997 / Accepted: 22 February 1997
About this article Cite this articleGübitz, G., Haltrich, D., Latal, B. et al. Mode of depolymerisation of hemicellulose by various mannanases and xylanases in relation to their ability to bleach softwood pulp. Appl Microbiol Biotechnol 47, 658–662 (1997). https://doi.org/10.1007/s002530050991
Issue Date: June 1997
DOI: https://doi.org/10.1007/s002530050991
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