The xylose reductase gene (XYL1) was isolated from Pichia stipitis and Candida shehatae, cloned into YEp-based vectors under the control of ADH2 and PGK1 promoter/terminator cassettes and introduced into Saccharomyces cerevisiae Y294 by electroporation. Shake-flask fermentations were carried out with 5% xylose and 1% galactose, glucose or maltose as co-substrates. Xylose uptake was similar in both the recombinant strains when different co-substrates were used and slowed once the co-substrate was depleted. The recombinant strains converted xylose to xylitol with yields approaching the theoretical maxima. Xylitol production was most rapid when the co-substrate was still present. Approximately 50% of the xylose was not metabolized due to the depletion of the co-substrate.
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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. Author information Authors and AffiliationsDepartment of Microbiology, University of Durban-Westville, P. Bag X54001, Durban, 4000, South Africa Tel.: +27-21-8083610 Fax: +27-21-8083611, , , , , , ZA
R. Govinden, B. Pillay & D. Pillay
Department of Microbiology, University of Stellenbosch, Stellenbosch 7600, South Africa, , , , , , ZA
W. H. van Zyl
Received: 23 December 1999 / Received revision: 30 June 2000 / Accepted: 1 July 2000
About this article Cite this articleGovinden, R., Pillay, B., van Zyl, W. et al. Xylitol production by recombinant Saccharomyces cerevisiae expressing the Pichia stipitis and Candida shehatae XYL1 genes. Appl Microbiol Biotechnol 55, 76–80 (2001). https://doi.org/10.1007/s002530000455
Issue Date: January 2001
DOI: https://doi.org/10.1007/s002530000455
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