Home - News ( United States | United Kingdom | Italy | Germany ) - Football scores

Showing content from https://link.springer.com/doi/10.1007/s002530000422 below:

Loss of glucose repression in an Acremonium chrysogenumβ-lactam producer strain and its restoration by multiple copies of the cre1 gene

In the filamentous fungus Acremonium chrysogenum, biosynthesis of the β-lactam antibiotic cephalosporin C is repressed by glucose. A wild-type strain grown in the presence of glucose shows a reduction of transcripts derived from the pcbC and cefEF biosynthesis genes. Interestingly, the amount of the pcbC transcript is not affected in another strain with enhanced cephalosporin C production, suggesting a correlation between strain improvement and deregulation of glucose repression. The function of the glucose repressor CRE1 in this regulation was further analyzed by transforming both A. chrysogenum strains with multiple copies of the cre1 gene. The molecular analysis of transformants revealed that additional copies of the cre1 gene restore wild-type-like regulation of pcbC gene expression in the semiproducer strain, while repression of the cefEF gene expression is increased. Overall, our data indicate a regulation of the pcbC and the cefEF gene expression by CRE1.

This is a preview of subscription content, log in via an institution to check access.

Springer+ Basic

€34.99 /Month

• Get 10 units per month
• Download Article/Chapter or eBook
• 1 Unit = 1 Article or 1 Chapter
• Cancel anytime

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

1. Lehrstuhl für Allgemeine und Molekulare Botanik, D-44780 Bochum, Germany e-mail: Ulrich.Kueck@ruhr-uni-bochum.de Fax: +49-234-3214184, , , , , , DE

   K. Jekosch & U. Kück

1. K. Jekosch
2. U. Kück

Received: 8 January 2000 / Received revision: 18 April 2000 / Accepted: 1 May 2000

Jekosch, K., Kück, U. Loss of glucose repression in an Acremonium chrysogenumβ-lactam producer strain and its restoration by multiple copies of the cre1 gene. Appl Microbiol Biotechnol 54, 556–563 (2000). https://doi.org/10.1007/s002530000422

Download citation

• Issue Date: October 2000

• DOI: https://doi.org/10.1007/s002530000422

RetroSearch is an open source project built by @garambo | Open a GitHub Issue

Search and Browse the WWW like it's 1997 | Search results from DuckDuckGo

HTML: 3.2 | Encoding: UTF-8 | Version: 0.7.4