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Showing content from https://link.springer.com/doi/10.1007/s002030100271 below:

Identification and characterization of a periplasmic nitrate reductase in Azospirillum brasilense Sp245

The Azospirillum brasilense Sp245 napABC genes, encoding nitrate reductase activity, were isolated and sequenced. The derived protein sequences are very similar throughout the whole Nap segment to the NapABC protein sequences of Escherichia coli, Pseudomonas sp. G-179, Ralstonia eutropha, Rhodobacter sphaeroides, and Paracoccus denitrificans. Based on whole-cell nitrate reductase assays with the artificial electron donors benzyl viologen and methyl viologen, and assays with periplasmic cell-free extracts, it was concluded that the napABC-encoded enzyme activity in Azospirillum brasilense Sp245 corresponds to a periplasmic dissimilatory nitrate reductase, which was expressed under anoxic conditions and oxic conditions. A kanamycin-resistant Azospirillum brasilense Sp245 napA insertion mutant was constructed. The mutant still expressed assimilatory nitrate reductase activity, but was devoid of its periplasmic dissimilatory nitrate reductase activity.

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1. F.A. Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, Kard. Mercierlaan 92, 3001 Heverlee, Belgium, , , , ,

   Oda Steenhoudt, Veerle Keijers, Yaacov Okon & Jos Vanderleyden

1. Oda Steenhoudt
2. Veerle Keijers
3. Yaacov Okon
4. Jos Vanderleyden

Electronic Publication

Steenhoudt, O., Keijers, V., Okon, Y. et al. Identification and characterization of a periplasmic nitrate reductase in Azospirillum brasilense Sp245. Arch Microbiol 175, 344–352 (2001). https://doi.org/10.1007/s002030100271

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• Received: 28 December 2000

• Revised: 26 February 2001

• Accepted: 01 March 2001

• Issue Date: May 2001

• DOI: https://doi.org/10.1007/s002030100271

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