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RFLP mapping of the genes controlling hybrid breakdown in rice (Oryza sativa L.)

Abstract

 Complementary recessive genes hwd1 and hwd2 controlling hybrid breakdown (weakness of F2 and later generations) were mapped in rice using RFLP markers. These genes produce a plant that is shorter and has fewer tillers than normal plants when the two loci have only one or no dominant allele at both loci. A cultivar with two dominant alleles at the hwd1 locus and a cultivar with two dominant alleles at the hwd2 locus were crossed with a double recessive tester line. Linkage analysis was carried out for each gene independently in two F2 populations derived from these crosses. hwd1 was mapped on the distal region of rice genetic linkage map for chromosome 10, flanked by RFLP markers C701 and R2309 at a distance of 0.9 centiMorgans (cM) and 0.6 cM, respectively. hwd2 was mapped in the central region of rice genetic linkage map for chromosome 7, tightly linked with 4 RFLP markers without detectable recombination. The usefulness of RFLP mapping and map information for the genes controlling reproductive barriers are discussed in the context of breeding using diverse rice germplasm, especially gene introduction by marker-aided selection.

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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. Author information Authors and Affiliations
  1. Department of Genetic Resources I, National Institute of Agrobiological Resources, Kannondai 2-1-2, Tsukuba, Ibaraki, 305 Japan, and Institute of Agriculture and Forestry, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305 Japan, , , , , , JP

    S. Fukuoka

  2. Institute of Agriculture and Forestry, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305 Japan, , , , , , JP

    H. Namai

  3. Department of Genetic Resources I, National Institute of Agrobiological Resources, Kannondai 2-1-2, Tsukuba, Ibaraki, 305 Japan Fax: +81-298-38-7408 E-mail: okusan@abr.affrc.go.jp, , , , , , JP

    K. Okuno

Authors
  1. S. Fukuoka
  2. H. Namai
  3. K. Okuno
About this article Cite this article

Fukuoka, S., Namai, H. & Okuno, K. RFLP mapping of the genes controlling hybrid breakdown in rice (Oryza sativa L.). Theor Appl Genet 97, 446–449 (1998). https://doi.org/10.1007/s001220050915

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