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Molecular mapping of hybrid necrosis genes Ne1 and Ne2 in hexaploid wheat using microsatellite markers

Hybrid necrosis is the gradual premature death of leaves or plants in certain F₁ hybrids of wheat (Triticum aestivum L.), and it is caused by the interaction of two dominant complementary genes Ne1 and Ne2 located on chromosome arms 5BL and 2BS, respectively. To date, molecular markers linked to these genes have not been identified and linkage relationships of the two genes with other important genes in wheat have not been established. We observed that the F₁ hybrids from the crosses between the bread wheat variety ‘Alsen’ and four synthetic hexaploid wheat (SHW) lines (TA4152-19, TA4152-37, TA4152-44, and TA4152-60) developed at the International Maize and Wheat Improvement Center (CIMMYT) exhibited hybrid necrosis. This study was conducted to determine the genotypes of TA4152-60 and Alsen at the Ne1 and Ne2 loci, and to map the genes using microsatellite markers in backcross populations. Genetic analysis indicated that Alsen has the genotype ne1ne1Ne2Ne2 whereas the SHW lines have Ne1Ne1ne2ne2. The microsatellite marker Xbarc74 was linked to Ne1 at a genetic distance of 2.0 cM on chromosome arm 5BL, and Xbarc55 was 3.2 cM from Ne2 on 2BS. Comparison of the genetic maps with the chromosome deletion-based physical maps indicated that Ne1 lies in the proximal half of 5BL, whereas Ne2 is in the distal half of 2BS. Genetic linkage analysis showed that Ne1 was about 35 cM proximal to Tsn1, a locus conferring sensitivity to the host selective toxin Ptr ToxA produced by the tan spot fungus. The closely linked microsatellite markers identified in this study can be used to genotype parental lines for Ne1 and Ne2 or to eliminate the two hybrid necrosis genes using marker-assisted selection.

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We thank Dr. Bikram S. Gill of WGRC at Kansas State University in Manhattan, KS, for providing synthetic wheat lines and S.W. Meinhardt, Department of Chemistry, North Dakota State University, Fargo, ND for providing Ptr ToxA used in this study. We thank Drs. Xiwen Cai and Lynn L. Dahleen for critical review of this manuscript. This research was supported by USDA-ARS CRIS Projects No. 5442-22000-026-00D and 5442-22000-030-00D.

1. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58105, USA

   C.-G. Chu

2. USDA-ARS Cereal Crops Research Unit, Northern Crop Science Laboratory, 1307, 18th Street North, Fargo, ND, 58105, USA

   J. D. Faris, T. L. Friesen & S. S. Xu

1. C.-G. Chu
2. J. D. Faris
3. T. L. Friesen
4. S. S. Xu

Correspondence to S. S. Xu.

Communicated by B. Keller

Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.

Chu, CG., Faris, J.D., Friesen, T.L. et al. Molecular mapping of hybrid necrosis genes Ne1 and Ne2 in hexaploid wheat using microsatellite markers. Theor Appl Genet 112, 1374–1381 (2006). https://doi.org/10.1007/s00122-006-0239-9

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• Received: 03 November 2005

• Accepted: 05 February 2006

• Published: 04 March 2006

• Issue Date: May 2006

• DOI: https://doi.org/10.1007/s00122-006-0239-9

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