Three previously published resistance gene analogues (RGAs), pic13, pic21 and pic19, were mapped in relation to sugarcane mosaic virus (SCMV) resistance genes (Scmv1, Scmv2) in maize. We cloned these RGAs from six inbreds including three SCMV-resistant lines (D21, D32, FAP1360A) and three SCMV-susceptible lines (D145, D408, F7). Pairwise sequence alignments among the six inbreds revealed a frequency of one single nucleotide polymorphism (SNP) per 33 bp for the three RGAs, indicating a high degree of polymorphism and a high probability of success in converting RGAs into codominant cleaved amplified polymorphic sequence (CAPS) markers compared to other sequences. SNPs were used to develop CAPS markers for mapping of the three RGAs in relation to Scmv1 (chromosome 6) and Scmv2 (chromosome 3), and for pedigree analyses of resistant inbred lines. By genetic mapping pic21 was shown to be different from Scmv2, whereas pic19 and pic13 are still candidates for Scmv1 and Scmv2, respectively, due to genetic mapping and consistent restriction patterns of ancestral lines.
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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. Author information Authors and AffiliationsInstitute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, Fruwirthstraße 21, 70593 Stuttgart, Germany, , , , ,
M. Quint, R. Mihaljevic, C. Dussle, M. Xu, A. Melchinger & T. Lübberstedt
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About this article Cite this articleQuint, .M., Mihaljevic, .R., Dussle, .C. et al. Development of RGA-CAPS markers and genetic mapping of candidate genes for sugarcane mosaic virus resistance in maize. Theor Appl Genet 105, 355–363 (2002). https://doi.org/10.1007/s00122-002-0953-x
Received: 23 September 2001
Accepted: 12 December 2001
Issue Date: August 2002
DOI: https://doi.org/10.1007/s00122-002-0953-x
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