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Structures of vesicular stomatitis virus glycoprotein: membrane fusion revisited

Structures of vesicular stomatitis virus glycoprotein: membrane fusion revisited

Abstract.

Glycoprotein G of the vesicular stomatitis virus (VSV) is involved in receptor recognition at the host cell surface and then, after endocytosis of the virion, triggers membrane fusion via a low pH-induced structural rearrangement. G is an atypical fusion protein, as there is a pH-dependent equilibrium between its pre- and post-fusion conformations. The atomic structures of these two conformations reveal that it is homologous to glycoprotein gB of herpesviruses and that it combines features of the previously characterized class I and class II fusion proteins. Comparison of the structures of G pre- and postfusion states shows a dramatic reorganization of the molecule that is reminiscent of that of paramyxovirus fusion protein F. It also allows identification of conserved key residues that constitute pH-sensitive molecular switches. Besides the similarities with other viral fusion machineries, the fusion properties and structures of G also reveal some striking particularities that invite us to reconsider a few dogmas concerning fusion proteins.

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  1. S. Roche

    Present address: Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany

Authors and Affiliations
  1. CNRS, UMR2472, INRA, UMR1157, IFR 115, Virologie Moléculaire et Structurale, 91198, Gif sur Yvette, France

    S. Roche, A. A. V. Albertini, J. Lepault, S. Bressanelli & Y. Gaudin

Authors
  1. S. Roche
  2. A. A. V. Albertini
  3. J. Lepault
  4. S. Bressanelli
  5. Y. Gaudin
Corresponding author

Correspondence to Y. Gaudin.

Additional information

Received 21 November 2007; received after revision 29 January 2008; accepted 30 January 2008

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Open Access This is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License ( https://creativecommons.org/licenses/by-nc/2.0 ), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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Roche, S., Albertini, A.A.V., Lepault, J. et al. Structures of vesicular stomatitis virus glycoprotein: membrane fusion revisited. Cell. Mol. Life Sci. 65, 1716–1728 (2008). https://doi.org/10.1007/s00018-008-7534-3

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