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A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis

Extraction of high-quality genomic DNA fromGossypium (cotton) species is difficult due to high levels of polysaccharide, oxidizable quinones, and other interfering substances. We describe a procedure that consistently permits isolation of cotton genomic DNA of satisfactory size and quality for RFLP and PCR analysis, as well as for most routine cloning applications. Several antioxidants, phenol-binding reagents, and phenol oxidase inhibitors are used throughout the procedure, and most polysaccharides are eliminated early in the procedure by isolation of nuclei.

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CIA:

chloroform-isoamyl alcohol

CTAB:

hexadecyltrimethyl-ammonium bromide

DIECA:

diethyldithiocarbamic acid

RAPD:

random amplified polymorphic DNA

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1. Andrew H. Paterson

   Present address: Department of Soil and Crop Sciences, Texas A & M University, 77843-2474, College Station, TX, USA

1. Department of Soil and Crop Sciences, Texas A & M University, 77843-2474, College Station, TX, USA

   Jonathan F. Wendel

2. Department of Botany, Iowa State University, 50011, Ames, IA, USA

   Curt L. Brubaker

1. Andrew H. Paterson
2. Curt L. Brubaker
3. Jonathan F. Wendel

Paterson, A.H., Brubaker, C.L. & Wendel, J.F. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Biol Rep 11, 122–127 (1993). https://doi.org/10.1007/BF02670470

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• Issue Date: June 1993

• DOI: https://doi.org/10.1007/BF02670470

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