We have investigated the potential for using cyclosporin A to increase the efficiency with which Epstein-Barr virus-transformed human lymphoblast lines can be prepared. Use of this immunosuppressive drug has permitted the development of a procedure with success rates exceeding 95% despite the processing of very large numbers of samples.
This is a preview of subscription content, log in via an institution to check access.
Access this article Subscribe and saveSpringer+ Basic
€34.99 /Month
Price includes VAT (Germany)
Instant access to the full article PDF.
Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. ReferencesMiller, G.; Lipman, M. Release of infectious Epstein-Barr virus by transformed marmoset leukocytes. Proc. Natl. Acad. Sci. USA 70: 190–194; 1973.
Gusella, J.; Wexler, N.; Conneally, M.; Naylor, S.; Anderson, M.; Tanzi, R.; Watkins, P.; Ottina, K.; Wallace, M.; Sakaguchi, A.; Young, A.; Shoulson, I.; Bonilla, E.; Martin, J. A polymorphic DNA marker genetically linked to Huntington's disease. Nature 306: 234–238; 1983.
Bird, A.; McLachlan, S.; Britton, S. Cyclosporin A promotes spontaneous outgrowth in vitro of Epstein-Barr virus-induced B-cell lines. Nature 289: 300–301; 1981.
Leapman, S.; Filo, R.; Smith, E.; Smith, P. Differential effects of cyclopsorin A on lymphocyte subpopulations. Transplant. Proc. 13: 405–409; 1981.
Moss, D.; Rickinson, A.; Pope, J. Long-term T-cell-mediated immunity to Epstein-Barr virus in man. III. Activation of cytotoxic T cells in virus-infected leukocyte cultures. Int. J. Cancer 23: 618–625; 1979.
Genetics Unit and Neurology Service, Massachusetts General Hospital, Boston, Massachusetts
Mary Anne Anderson & James F. Gusella
Department of Genetics, Harvard Medical School, Boston, Massachusetts
Mary Anne Anderson & James F. Gusella
This work was supported by National Institute of Neurological and Communicative Disorders and Stroke Grants NS16367 (Huntington's Disease Center Without Walls) and NS 20012.
About this article Cite this articleAnderson, M.A., Gusella, J.F. Use of cyclosporin a in establishing epstein-barr virus-transformed human lymphoblastoid cell lines. In Vitro 20, 856–858 (1984). https://doi.org/10.1007/BF02619631
Received: 12 April 1984
Accepted: 02 July 1984
Issue Date: November 1984
DOI: https://doi.org/10.1007/BF02619631
RetroSearch is an open source project built by @garambo | Open a GitHub Issue
Search and Browse the WWW like it's 1997 | Search results from DuckDuckGo
HTML:
3.2
| Encoding:
UTF-8
| Version:
0.7.4