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Isolation and characterization ofMethanococcus mazei

Methanococcus mazei strain S-6 was isolated from a laboratory digester; this is the first report of its isolation in pure culture. The present isolate exhibited a life cycle, and the forms produced during its development fit the morphological description ofM. mazei exactly. In young cultures, cell clusters resembleMethanosarcina strain TM-1; in older cultures, these cell clusters become “cysts” which may be physically ruptured to yield myriads of highly irregular coccoid elements.M. mazei used acetate, methanol, methylamine, or trimethylamine for growth and methanogenesis. It used H₂−CO₂ slowly. It did not use formate, ethanol, butyrate, or propionate. It was Gram negative to Gram variable.

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1. Division of Environmental & Nutritional Sciences, School of Public Health, University of California, 90024, Los Angeles, California, USA

   Robert A. Mah

Mah, R.A. Isolation and characterization ofMethanococcus mazei . Current Microbiology 3, 321–326 (1980). https://doi.org/10.1007/BF02601895

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• Issue Date: November 1980

• DOI: https://doi.org/10.1007/BF02601895

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