Results demonstrating involvement of glycoprotein C (gC) of herpes simplex type 1 virus (HSV-1) in attachment of the virus to the cell are presented. Monoclonal antibodies against gC-1 inhibited adsorption of gC+-strains. The gC−-mutant, MP, attached to cells but at a reduced rate. Attachment of the MP-mutant was unaffected by presence of anti-gC-1 antibody. Purified truncated gC-1 adsorbed to cells at a rate essentially the same as that of gC+-virus. Glycoprotein C-1 pretreated with heparin did not adsorb to cells. The results are compatible with a suggested role for gC in HSV attachment.
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Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. ReferencesCai W, Person S, Werner SC, Zhou JH, Deluca NA (1987) Linker-insertion nonsense and restriction site deletion mutations of the gB glycoprotein gene of herpes simplex virus type 1. J Virol 61: 714–721
Campadelli-Fiume G, Stirpe D, Boscaro A, Avitabile E, Foa-Tomasi L, Barker D, Roizman B (1990) Glycoprotein C-dependent attachment of herpes simplex virus to susceptible cells leading to productive infection. Virology 178: 213–222
Desai PJ, Schaffer PA, Minson AC (1988) Excretion of non-infectious virus particles lacking glycoprotein H by a temperature-sensitive mutant of herpes simplex virus type 1: evidence that gH is essential for virion infectivity. J Gen Virol 69: 1147–1156
Fuller OA, Spear PG (1985) Specificities of monoclonal and polyclonal antibodies that inhibit adsorption of herpes simplex virus to cells and lack of inhibition by potent neutralizing antibodies. J Virol 55: 475–482
Jeansson S, Forsgren M, Svennerholm B (1983) Evaluation of solubilized herpes simplex virus membrane antigen by enzyme-linked immunosorbent assay. J Clin Microbiol 18: 1160–1166
Kaner RJ, Baird A, Mansukhani A, Basilico C, Summers BD, Florkiewicz RZ, Hajjar DP (1990) Fibroblast growth factor receptor is a portal of cellular entry for herpes simplex virus type 1. Science 248: 1410–1413
Kuhn JE, Kramer MD, Willenbacher W, Wieland U, Lorentzen EU, Braun RW (1990) Identification of herpes simplex virus type 1 glycoproteins interacting with the cell surface. J Virol 64: 2491–2497
Langeland N, Holmsen H, Lillehaug JR, Haarr L (1987) Evidence that Neomycin inhibits binding of herpes simplex virus type 1 to the cellular receptor. J Virol 61: 3388–3393
Langeland N, Oyan AM, Marsden HS, Cross A, Glorioso JC, Moore LJ, Haarr L (1988) Interaction of polylysine with the cellular receptor for herpes simplex virus type 1. J Gen Virol 69: 1137–1145
Ligas MW, Johnson DC (1988) A herpes simplex virus mutant in which glycoprotein D sequence are replaced by β-galactosidase sequences binds to but is unable to penetrate into cells. J Virol 62: 1486–1494
Longnecker R, Roizman B (1987) Clustering of genes dispensible for growth in culture in the S component of the HSV-1 genome. Science 236: 573–576
Lycke E, Johansson M, Svennerholm B, Lindahl U (1991) Binding of herpes simplex virus to cellular heparan sulphate, an initial step in the adsorption process. J Gen Virol 72: 1131–1137
Mettenleiter TC, Zsak L, Zuckermann F, Sugg N, Kern H, Ben-Porat T (1990) Interaction of glycoprotein gIII with a cellular heparinlike substance mediates adsorption of pseudorabies virus. J Virol 64: 278–286
Neidhardt H, Schröder CH, Kaerner HC (1987) Herpes simplex virus type 1 glycoprotein E is not indispensible for viral infectivity. J Virol 61: 600–603
Sawitzky D, Hampl H, Habermehl K-O (1990) Comparison of heparin-sensitive attachment of pseudorabies virus (PRV) and herpes simplex virus type 1 and identification of heparin-binding PRV glycoproteins. J Gen Virol 71: 1221–1225
Vahlne A, Svennerholm B, Lycke E (1979) Evidence for herpes simplex virus type-selective receptors on plasma cellular membranes. J Gen Virol 44: 217–225
Weber PC, Levine M, Glorioso JC (1987) Rapid identification of nonessential genes of herpes simplex virus type 1 by Tn5 mutagenesis. Science 263: 576–579
WuDunn D, Spear PG (1989) Initial interaction of herpes simplex virus with cells is binding to heparan sulphate. J Virol 63: 52–58
Department of Clinical Virology, University of Göteborg, Göteborg, Sweden
B. Svennerholm, S. Jeansson, A. Vahlne & E. Lycke
Svennerholm, B., Jeansson, S., Vahlne, A. et al. Involvement of glycoprotein C (gC) in adsorption of herpes simplex virus type 1 (HSV-1) to the cell. Archives of Virology 120, 273–279 (1991). https://doi.org/10.1007/BF01310482
Received: 03 December 1990
Accepted: 19 April 1991
Issue Date: September 1991
DOI: https://doi.org/10.1007/BF01310482
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