Home - News ( United States | United Kingdom | Italy | Germany ) - Football scores

Showing content from https://link.springer.com/doi/10.1007/BF00693408 below:

Utilization of formate as an additional energy source by glucose-limited chemostat cultures ofCandida utilis CBS 621 andSaccharomyces cerevisiae CBS 8066

Candida utilis CBS 621 andSaccharomyces cerevisiae CBS 8066 were grown in glucose-limited chemostat cultures with formate as an additional energy source. In both yeasts formate was oxidized via a cytoplasmic NAD⁺-linked formate dehydrogenase. Other formate-oxidizing enzymes could not be detected.

WithCandida utilis the steady-state cell yield on glucose increased with increasing amounts of formate in the medium until growth became carbon-limited. The maximum growth yield on glucose in the presence of excess formate was dependent on the nitrogen source used for growth. With ammonium and nitrate the maximum yields were 0.69 and 0.56 g cells/g glucose, respectively. Calculations showed that this difference correlates with the NADPH requirement for biomass formation with these two nitrogen sources. This implies that the NADH produced from formate oxidation cannot replace the NADPH needed for biomass formation. It therefore is concluded that inCandida utilis transhydrogenase activity is absent.

AlsoSaccharomyces cerevisiae was capable of oxidizing formate in glucose-limited chemostat cultures. However, in contrast toCandida utilis utilization of formate by this yeast did not enhance the cell yield on glucose.

This is a preview of subscription content, log in via an institution to check access.

Springer+ Basic

€34.99 /Month

• Get 10 units per month
• Download Article/Chapter or eBook
• 1 Unit = 1 Article or 1 Chapter
• Cancel anytime

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

• Babel W, Müller RH, Markuske KD (1983) Improvement of growth yield of yeast on glucose to the maximum by using an additional energy source. Arch Microbiol 136:203–208

  Google Scholar 

• Bruinenberg PM, van Dijken JP, Scheffers WA (1983a) A theoretical analysis of NADPH production and consumption in yeasts. J Gen Microbiol 129:953–964

  Google Scholar 

• Bruinenberg PM, van Dijken JP, Scheffers WA (1983b) An enzymic analysis of NADPH production and consumption inCandida utilis. J Gen Microbiol 129:965–971

  Google Scholar 

• Dijkhuizen L, Groen L, Harder W, Konings WN (1977) Active transport of oxalate byPseudomonas oxalaticus OX1. Arch Microbiol 115:223–227

  Google Scholar 

• Dijkhuizen L, Timmerman JWC, Harder W (1979) A pyridine nucleotide-independent membrane-bound formate dehydrogenase inPseudomonas oxalaticus OX1. FEMS Microbiol Lett 6:53–56

  Google Scholar 

• Harder W, Visser K, Kuenen JG (1974) Laboratory fermenter with an improved magnetic driye. Lab Practice 23:644–645

  Google Scholar 

• Hazeu W, Donker RA (1983) A continuous culture study of methanol and formate utilization by the yeastPichia pastoris. Biotechn Lett 5:399–404

  Google Scholar 

• Lang E, Lang H (1972) Spezifische Farbreaktion zum direkten Nachweis der Ameisensäure. Z Anal Chem 260:8–10

  Google Scholar 

• Lloyd D (1974) The mitochondria of microorganisms. Academic Press. London New York San Francisco

  Google Scholar 

• Oura E (1972) The effect of aeration on the growth energetics and biochemical composition of baker's yeast. Ph.D. thesis, University of Helsinki

• Sahm H, Roggenkamp R, Wagner F, Hinkelmann W (1975) Microbodies in methanol-grownCandida boidinii. J Gen Microbiol 88:218–222

  Google Scholar 

• Tanaka A, Yasuhara S, Kawamoto S, Fukui S, Osumi M (1976) Development of microbodies in the yeastKloeckera growing on methanol. J Bacteriol 126:919–927

  Google Scholar 

• Van Dijken JP, Harder W (1975) Growth yields of microorganisms on methanol and methane. A theoretical study. Biotechnol Bioeng 17:15–30

  Google Scholar 

• Van Verseveld HW, Boon JP, Stouthamer AH (1979) Growth yields and the efficiency of oxidative phosphorylation ofParacoccus denitrificans during two-(carbon)substrate-limited growth. Evidence for the induction of site 3 phosphorylation by methanol in heterotrophically grown cells. Arch Microbiol 121:213–223

  Google Scholar 

• Veenhuis M, van Dijken JP, Harder W (1983) The significance of peroxisomes in the metabolism of one-carbon compounds in yeasts. Adv Microbial Physiol 24:1–82

  Google Scholar 

• Von Jagow G, Klingenberg M (1970) Pathways of hydrogen in mitochondria ofSaccharomyces carlsbergensis. Eur J Biochem 12:583–592

  Google Scholar 

Download references

1. Laboratory of Microbiology, Delft University of Technology, Julianalaan 67A, NL-2628 BC, Delft, The Netherlands

   Peter M. Bruinenberg, Ronald Jonker, Johannes P. van Dijken & W. Alexander Scheffers

1. Peter M. Bruinenberg
2. Ronald Jonker
3. Johannes P. van Dijken
4. W. Alexander Scheffers

Bruinenberg, P.M., Jonker, R., van Dijken, J.P. et al. Utilization of formate as an additional energy source by glucose-limited chemostat cultures ofCandida utilis CBS 621 andSaccharomyces cerevisiae CBS 8066. Arch. Microbiol. 142, 302–306 (1985). https://doi.org/10.1007/BF00693408

Download citation

• Received: 04 January 1985

• Accepted: 16 May 1985

• Issue Date: August 1985

• DOI: https://doi.org/10.1007/BF00693408

RetroSearch is an open source project built by @garambo | Open a GitHub Issue

Search and Browse the WWW like it's 1997 | Search results from DuckDuckGo

HTML: 3.2 | Encoding: UTF-8 | Version: 0.7.4