In zwei voneinander unabhängigen Versuchen wurden insgesamt 120 Mäuse in zwei gleiche Gruppen eingeteilt, von denen die eine als Kontrollgruppe diente, die andere wurde mit Ehrlich Ascites Tumorzellen injiziert. Im ersten Versuch wurde bei vier Kontrolltieren und vier mit Tumorzellen injizierten Tieren am 1., 3., 5., 7. und 9. Tag nach der Injektion der Tumorzellen das Lipoprotein Muster im Plasma durch Disc Electrophorese bestimmt. Nach dem dritten, der Injektion folgenden Tag zeigte das elektrophoretische Muster der Ehrlich Ascites Tumor-tragenden Mäuse eine starke Abnahme der Lipoproteine hoher Dichte und eine Zunahme der Lipoproteine niedriger Dichte. Um diese Ergebnisse zu bestätigen, wurde in einem zweiten Versuch (40 Kontrollen und 40 Ehrlich Ascites Tumor-tragende Mäuse) die Lipoproteine hoher Dichte des Plasmas durch elektrophoretische Elution isoliert und ihr Phospholipidgehalt bestimmt. Eine signifikante Abnahme (70%, p. 0,01) des Phospholipidgehaltes der Lipoproteine hoher Dichte wurde nach dem dritten der Injeektion folgenden Tag gefunden. Sie läßt eine Beziehung zwischen dem Wachstum der Tumorzellen und dem Lipoproteinen hoher Dichte des Plasmas vermuten.
SummaryIn two separate experiments, a total of 120 mice were divided into two equal groups, one a control and the other an experimental group which was injected with Ehrlich ascites tumor cells. In the first experiment, the plasma lipoprotein patterns were obtained by disc electrophoresis from 4 control and 4 Ehrlich ascites tumor cell-injected animals on the 1st, 3rd, 5th, 7th and 9th day after injection of the tumor cells. After the 3rd day of injection, the electrophoretic patterns from Ehrlich ascites tumor-bearing mice indicated a striking decrease in high-density lipoproteins and an elevation in low-density lipoproteins. In order to confirm these results, in a second experiment (40 control and 40 Ehrlich ascites tumor-bearing mice), the plasma high-density lipoproteins were isolated by electrophoretic elution and their phospholipid was determined. A significant decrease (70%, p<0.01) in high-density lipoprotein-phospholipid was observed after the 3rd day of injection in the plasma of tumor-bearing mice and suggested a relationship between the growth of tumor cells and plasma high-density lipoproteins.
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Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. ReferencesBarclay, M., Skipski, V. P., Terebus-Kekish, O., Merker, P. L., Cappuccino, J. G.: Serum lipoproteins in rats with tumors induced by 9,10-dimethyl-1,2-benzanthracene and with transplanted Walker carcinosarcoma 256. Cancer Res. 27, 1158–1167 (1967).
Morgan, J. F., Bowman, B., Lippinski, F.: Studies on the cultivation of ascites tumor cells in chemically defined media. In: Cancer Cells in Culture, pp. 48–59. (Ed.: H. Katsta). University Park Press 1968.
Narayan, K. A.: Serum lipoproteins during chemical carcinogenesis. Biochem. J. 103, 672–676 (1967).
—: Serum lipoproteins of rats fed an essential fatty acid deficient diet and N-2-fluorenylacetamide. Cancer Res., 30, 1185–1191 (1970a).
Narayan, K. A.: Serum lipoproteins during chemical carcinogenesis. Paper submitted to the Tenth International Cancer Congress, Houston, Texas, May (1970b).
—, Creinin, H. L., Kummerow, F. A.: Disc electrophoresis of rat plasma lipoproteins. J. Lipid Res. 7, 150–157 (1966).
—, Mary, G. E. S., Kummerow, F. A.: Rat serum lipoproteins after partial hepatectomy. Proc. Soc. exp. Biol. (N.Y.) 129, 6–12 (1968).
—, Morris, H. P.: Serum lipoproteins of rats bearing transplanted Morris hepatoma 7777. Int. J. Cancer, 5, 410–414 (1970).
Rejnek, J., Bednarik, T., Rerabkova, E., Peskova, D.: The effect of human serum fractions on cell growth and its relation to the state of alpha1-lipoprotein. Exp. Cell Res. 37, 65–78 (1965).
Riley, V.: Adaptation of orbital bleeding technic to rapid serial blood studies. Proc. Soc. exp. Biol. (N.Y.) 104, 751–754 (1960).
The Burnsides Research Laboratory, University of Illinois, 61801, Urbana, Illinois
Howard L. Creinin & K. Ananth Narayan
This work was supported by a research grant CAO-1932 from USPHS and a research grant from the American Cancer Society. The authors thank Dr. P. Klein and Dr. A. N. Stroud of Argone National Laboratory, Illinois, for the initial supply of Ehrlich ascites tumor cells. Appreciation is due to Mrs. Linda Williams for her valuable assistance in the disc electrophoretic experiments.
Part of a Ph. D thesis in preparation by H. L. Creinin.
Research Career Development Awardee, 5K3-CA-31,063 from the National Cancer Institute, USPHS.
About this article Cite this articleCreinin, H.L., Narayan, K.A. Effect of Ehrlich ascites tumor cells on mouse plasma lipoproteins. Z Krebs-forsch 75, 93–98 (1971). https://doi.org/10.1007/BF00525929
Received: 30 April 1970
Accepted: 04 June 1970
Issue Date: June 1971
DOI: https://doi.org/10.1007/BF00525929
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