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Glutathione reductase activity in heterocysts and vegetative cells of the cyanobacterium Nostoc muscorum

Glutathione reductase activity was detected and characterized in heterocysts and vegetative cells of the cyanobacterium Nostoc muscorum. The activity of the enzyme varied between 50 and 150 nmol reduced glutathione· min^-1·mg protein^-1, and the apparent K_m for NADPH was 0.125 and 0.200 mM for heterocysts and vegetative cells, respectively. The enzyme was found to be sensitive to Zn⁺² ions, however, preincubation with oxidized glutathione rendered its resistance to Zn⁺² inhibition. Nostoc muscorum filaments were found to contain 0.6–0.7mM glutathione, and it is suggested that glutathione reductase can regenerate reduced glutathione in both cell types. The combined activity of glutathione reductase and isocitrate dehydrogenase in heterocysts was as high as 18 nmol reduced glutathione·min^-1·mg protein^-1. A relatively high superoxide dismutase activity was found in the two cell types; 34.2 and 64.3 enzyme units·min^-1·mg protein^-1 in heterocysts and vegetative cells, respectively.

We suggest that glutathione reductase plays a role in the protection mechanism which removes oxygen radicals in the N₂-fixing cyanobacterium Nostoc muscorum.

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DTNB:

5-5′-dithiobis-(2-nitrobenzoic acid)

EDTA:

ethylenediaminetetra-acetic acid

GR:

glutathione reductase (EC1.6.4.2)

GSH:

reduced glutathione

GSSG:

oxidized glutathione

OPT:

O-phtaldialdehyde

SOD:

superoxide dismutase (EC 1.15.1.1)

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1. Department of Agricultural Botany, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, 76100, Rehovot, Israel

   Leah Karni, Stephen J. Moss & Elisha Tel-Or

1. Leah Karni
2. Stephen J. Moss
3. Elisha Tel-Or

Karni, L., Moss, S.J. & Tel-Or, E. Glutathione reductase activity in heterocysts and vegetative cells of the cyanobacterium Nostoc muscorum . Arch. Microbiol. 140, 215–217 (1984). https://doi.org/10.1007/BF00454930

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• Received: 26 January 1984

• Accepted: 27 July 1984

• Issue Date: December 1984

• DOI: https://doi.org/10.1007/BF00454930

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