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Role of peroxidase in the development of water-impermeable seed coats in Sida spinosa L.

The seed coats of S. spinosa (prickly sida, Malvaceae) become impermeable to water during seed development on the mother plant. After the seeds have dehydrated during the final maturation stages, piercing of seed coats is necessary to induce imbibition of water and germination. Onset of impermeability occurs during seed coat browning, well in advance of seed dehydration. I. Marbach and A.M. Mayer (1975, Plant Physiol. 56, 93–96) implicated polyphenol oxidase (PO; EC 1.10.3.1) as catechol oxidase in the formation of insoluble polymers during development of coat impermeability in a wild strain of pea (Pisum elatius) seeds. We found, however, that peroxidase (EC 1.11.1.7), not PO, is instrumental in the development of water-impermeable seed coats in prickly sida. We isolated coats and embryos from seeds harvested at several stages of development. Highest peroxidase activity of coat extracts correlated well with the developmental stages of maximum conversion of soluble phenolics to insoluble lignin polymers. Although seed extracts oxidized dihydroxyphenylalanine, this activity was eliminated by catalase, indicating that the oxidation of phenolics in the coat is catalyzed by peroxidase rather than PO. Histochemical localization of peroxidase was strongest in the palisade layer; both the level and time of appearance of activity was proportional to the spectrophotometric assays of seed-coat extracts. The presence of peroxidase and the absence of PO in the seed coat were also confirmed with immunocytochemistry. Our results support the view that peroxidase is involved in the polymerization of soluble phenolics to insoluble lignin polymers during development of prickly sida seed coats, causing the formation of a water-impermeable barrier prior to seed dehydration. As dehydration proceeds, the chalazal area finally becomes impermeable resulting in the hard mature seeds of prickly sida.

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DAB:

diaminobenzidine

DOPA:

dihydroxyphenylalanine

PO:

polyphenol oxidase

• Ballard, L.A.T. (1973) Physical barriers to germination. Seed Sci. Technol. 1, 285–303

  Google Scholar 

• Barton, L.V. (1965) Dormancy in seeds imposed by the seed coat. In: Encyclopedia of plant physiology, vol. 15, pt. 2, pp. 727–745, Ruhland, W., ed. Springer, Berlin Heidelberg New York

  Google Scholar 

• Berlyn, G.P., Miksche, J.P. (1976) Botanical microtechnique and cytochemistry. Iowa State University Press, Ames, USA

  Google Scholar 

• Blume, D.E., McClure, J.W. (1980) Developmental effects of Sandoz 6706 on activities of enzymes of phenolic and general metabolism in barley shoots grown in the dark or under high or low intensity light. Plant Physiol. 65, 238–244

  Google Scholar 

• Corner, E.J.H. (1976) The seeds of dicotyledons, vol. 1. Cambridge University Press, Cambridge

  Google Scholar 

• Duke, S.O., Vaughn, K.C. (1982) Lack of involvement of polyphenol oxidase in ortho-hydroxylation of phenolic compounds in mung bean seedlings. Physiol. Plant 54, 381–385

  Google Scholar 

• Egley, G.H. (1976) Germination of developing prickly sida seeds. Weed Sci. 24, 239–243

  Google Scholar 

• Egley, G.H., Chandler, J.M. (1978) Germination and viability of weed seeds after 2.5 years in a 50-year buried seed study. Weed Sci. 26, 230–239

  Google Scholar 

• Egley, G.H., Paul, R.N., Jr. (1981) Morphological observations on the early imbibition of water by Sida spinosa (Malvaceae) seed. Am. J. Bot. 68, 1056–1065

  Google Scholar 

• Egley, G.H., Paul, R.N., Jr. (1982) Development, structure and function of subpalisade cells in water impermeable Sida spinosa seeds. Am. J. Bot. 69, 1402–1409

  Google Scholar 

• Gross, G.G. (1977) Biosynthesis of lignin and related monomers. Rec. Adv. Phytochem. 11, 141–184

  Google Scholar 

• Harkin, J.M., Obst, J.R. (1973) Lignification in trees: indication of exclusive peroxidase participation. Science 180, 296–298

  Google Scholar 

• Hoagland, R.E., Duke, S.O., Elmore, C.D. (1978) Effects of glyphosate on metabolism of phenolic compounds. II. Influence on soluble hydroxyphenolic compounds, free amino acids and soluble protein levels on dark-grown maize roots. Plant Sci. Lett. 13, 291–299

  Google Scholar 

• Marbach, I., Mayer, A.M. (1974) Permeability of seed coats to water as related to drying conditions and metabolism of phenolics. Plant Physiol. 54, 817–820

  Google Scholar 

• Marbach, I., Mayer, A.M. (1975) Changes in catechol oxidase and permeability to water in seed coats of Pisum elatius during seed development and maturation. Plant Physiol. 56, 93–96

  Google Scholar 

• Olah, A.F., Mueller, W.C. (1981) Ultrastructural localization of oxidative and peroxidative activities in a carrot suspension cell culture. Protoplasma 106, 231–248

  Google Scholar 

• Postek, M.T., Tucker, S.C. (1976) A new short chemical dehydration method for light microscopy preparations of plant material. Can. J. Bot. 54, 872–875

  Google Scholar 

• Rolston, M.P. (1978) Water impermeable seed dormany. Bot. Rev. 44, 365–396

  Google Scholar 

• Sexton, R., Hall, J.L. (1978) Enzyme cytochemistry. In: Electron microscopy and cytochemistry of plant cells, pp. 63–147, Hall, J.L., ed. Elsevier/North-Holland, Amsterdam

  Google Scholar 

• Spurr, A.R. (1969) A low-viscosity epoxy embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43

  Google Scholar 

• Stafford, H.A. (1960) Comparison of lignin-like polymers produced peroxidatively by cinnamic acid derivatives in leaf sections of Phleum. Plant Physiol. 35, 612–618

  Google Scholar 

• Stafford, H.A. (1974) The metabolism of aromatic compounds. Annu. Rev. Plant Physiol. 25, 459–486

  Google Scholar 

• Sternberger, L.A. (1979) Immunocytochemistry. Wiley, New York

  Google Scholar 

• Vaughn, K.C., Duke, S.O. (1981a) Tissue localization of polyphenol oxidase in sorghum. Protoplasma 108, 319

  Google Scholar 

• Vaughn, K.C., Duke, S.O. (1981b) Tentoxin-induced loss of plastidic polyphenol oxidase. Physiol. Plant. 53, 421–428

  Google Scholar 

• Werker, E. (1980–1981) Seed dormancy as explained by the anatomy of embryo envelopes. Isr. J. Bot. 29, 22–44

  Google Scholar 

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1. Southern Weed Science Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 38776, Stoneville, MS, USA

   G. H. Egley, R. N. Paul Jr., K. C. Vaughn & S. O. Duke

1. G. H. Egley
2. R. N. Paul Jr.
3. K. C. Vaughn
4. S. O. Duke

Egley, G.H., Paul, R.N., Vaughn, K.C. et al. Role of peroxidase in the development of water-impermeable seed coats in Sida spinosa L.. Planta 157, 224–232 (1983). https://doi.org/10.1007/BF00405186

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• Received: 26 July 1982

• Accepted: 10 November 1982

• Issue Date: April 1983

• DOI: https://doi.org/10.1007/BF00405186

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