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Showing content from https://link.springer.com/doi/10.1007/BF00391811 below:

Extrachromosomal genetics of Cephalosporium acremonium

DNA analysis of Cephalosporium acremonium revealed that this important fungus for biotechnology contains, apart from chromosomal DNA, two types of extrachromosomal DNA which may be useful for the development of a homologous vector system:

1. 1.

   Plasmid-like (pl) DNA consisting of circular molecules, but of rather heterogeneous buoyant density and restriction pattern. In contrast to Podospora pl DNA it is not associated with mitochondrial DNA.

2. 2.

   Mitochondrial (mt) DNA consisting of the smallest circular molecules (26.7 kb) known so far in filamentous fungi. It contains unique cleavage sites for at least 3 restriction endonucleases.

A detailed physical map of mtDNA was constructed. A genetics map was also established by hybridization with rho⁻ DNA of Saccharomyces cerevisiae. Thus sufficient information for the construction of a mitochondrial vector is now available.

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1. Walter Minut

   Present address: Millipore GmbH, Siemensstr. 20, 6078, NeuIsenburg, Germany

1. Lehrstuhl für Allgemeine Botanik, Ruhr-Universität, Postfach 102148, D-4630, Bochum 1, Germany

   Walter Minut, Paul Tudzynsk & Karl Esser

1. Walter Minut
2. Paul Tudzynsk
3. Karl Esser

Minut, W., Tudzynsk, P. & Esser, K. Extrachromosomal genetics of Cephalosporium acremonium . Curr Genet 5, 227–231 (1982). https://doi.org/10.1007/BF00391811

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• Received: 03 May 1982

• Issue Date: August 1982

• DOI: https://doi.org/10.1007/BF00391811

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