A restriction fragment length polymorphism (RFLP) analysis was performed on six related Acremonium strains. With respect to the restriction fragment pattern, all strains of A. chrysogenum were indistinguishable from each other but showed distinctive differences from those of A. strictum, A. flavum and Cephalosporium polyvaleurum. Using pulsed-field gel electrophoresis, we obtained different chromosome patterns from most of the Acremonium strains. Remarkably, the pattern varies in three related A. chrysogenum strains which also differ in their rate of cephalosporin C biosynthesis. The electrophoretic karyotyping was confirmed by the location of rDNA genes on separate chromosomes. Our data indicate that chromosome translocations in industrial strains may be responsible for increased β-lactam synthesis.
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Markus Walz & Ulrich Kück
Communicated by K. Esser
About this article Cite this articleWalz, M., Kück, U. Polymorphic karyotypes in related Acremonium strains. Curr Genet 19, 73–76 (1991). https://doi.org/10.1007/BF00326285
Received: 15 October 1990
Issue Date: February 1991
DOI: https://doi.org/10.1007/BF00326285
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