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Differential fluorescent staining of Drosophila chromosomes with quinacrine mustard

The polytene and mitotic chromosomes of D. melanogaster, D. simulans, D. ananassae, and D. virilis were stained with the fluorescent dye, quinacrine mustard (QM). In all these species except D. ananassae, we have detected species-specific chromosomal loci which exhibit an extremely brilliant fluorescence. Most, but not all, of the brilliantly fluorescing areas are located in heterochromatic chromosome regions. Cytochemical and chemical methods have been employed to demonstrate that the brilliant fluorescence represents regions of acid labile non-covalent binding between DNA and QM whereas the moderate overall fluorescence is primarily due to covalent bonding (by alkylation) of the QM to DNA. The exact mode of binding of QM in the brilliant areas and the nature of the DNA in these areas are not known. The possible biological significance of the DNA in the brilliant regions is discussed.

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• Caspersson, T., Farber, S., Foley, G. E., Kudynowski, J., Modest, E. J., Simonsson, E., Wagh, U., Zech, L.: Chemical differentiation along metaphase chromosomes. Exp. Cell Res. 49, 219–222 (1968).

  Google Scholar 

• —, Zech, L., Johansson, C., Modest, E. J.: Identification of human chromosomes by DNA-binding fluorescent agents. Chromosoma (Berl.) 30, 215–227 (1970).

  Google Scholar 

• —, Modest, E. J., Foley, G. E., Wagh, U., Simonsson, E.: Chemical differentiation with fluorescent alkylating agents in Vicia faba metaphase chromosomes. Exp. Cell Res. 58, 128–140 (1969a).

  Google Scholar 

• —: DNA-binding fluorochromes for the study of the organization of the metaphase nucleus. Exp. Cell Res. 58, 141–152 (1969b).

  Google Scholar 

• Fujii, S.: Further studies on the salivary gland chromosomes of Drosophila virilis. Cytologia (Tokyo) 12, 435–453 (1942).

  Google Scholar 

• Hannah, A.: Localization and function of heterochromatin in Drosophila melanogaster. Advanc. Genet. 4, 87–121 (1951).

  Google Scholar 

• Heitz, E.: Das Heterochromatin der Moose. I. Jb. wiss. Bot. 69, 762–818 (1928).

  Google Scholar 

• Jones, K. W., Robertson, F. W.: Localisation of reiterated nucleotide sequences in Drosophila and mouse by in situ hybridization of complementary RNA. Chromosoma (Berl.) 31, 331–345 (1970).

  Google Scholar 

• Kaufmann, B. P.: Somatic mitoses of Drosophila melanogaster. J. Morph. 56, 125–155 (1934).

  Google Scholar 

• Laird, C. D., McCarthy, B. J.: Magnitude of interspecific nucleotide sequence variability in Drosophila. Genetics 60, 303–322 (1968).

  Google Scholar 

• Lawley, P. D.: Further studies of the action of alkylating agents on nucleic acids and nucleotides. Ann. Rep. British Empire Cancer Campaign 36, 16–18 (1958).

  Google Scholar 

• — Brookes, P.: Mechanism of action of alkylating agents. Ann. Rep. British Empire Cancer Campaign 38, 3–13 (1960).

  Google Scholar 

• —, Wallick, C. A.: The action of alkylating agents on deoxyribonucleic acid and guanylic acid. Chemistry and Industry 20, 633–635 (1957).

  Google Scholar 

• Lewis, E. B.: The phenomenon of position effect. Advanc. Genet. 3, 73–112 (1950).

  Google Scholar 

• Lima-de-Faria, A., Jaworska, H.: Late DNA synthesis in heterochromatin. Nature (Lond.) 217, 138–142 (1968).

  Google Scholar 

• Makino, S.: On the chromocentre observed through the mitotic cycle of somatic cells in Drosophila virilis. Cytologia (Tokyo) 10, 283–293 (1940).

  Google Scholar 

• Marmur, J.: A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. molec. Biol. 3, 208–218 (1961).

  Google Scholar 

• Rae, P. M. M.: Chromosomal distribution of rapidly reannealing DNA in Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 67, 1018–1025 (1970).

  Google Scholar 

• Vosa, C. G.: The discriminating fluorescence patterns of the chromosomes of Drosophila melanogaster. Chromosoma (Berl.) 31, 446–451 (1970).

  Google Scholar 

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1. Department of Zoology and Cytogenetics Laboratory of Carnegie Institution of Washington, The University of Michigan, Ann Arbor, Michigan

   K. P. Adkisson, W. J. Perreault & H. Gay

1. K. P. Adkisson
2. W. J. Perreault
3. H. Gay

This paper is dedicated with respect and affection to Professor Berwind P. Kaufmann on the occasion of his 74th birthday, April 23, 1971.

This study was supported by a Research Grant (GM 10499) from the National Institutes of Health, U.S. Public Health Service.

Adkisson, K.P., Perreault, W.J. & Gay, H. Differential fluorescent staining of Drosophila chromosomes with quinacrine mustard. Chromosoma 34, 190–205 (1971). https://doi.org/10.1007/BF00285186

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• Received: 10 May 1971

• Accepted: 13 May 1971

• Issue Date: June 1971

• DOI: https://doi.org/10.1007/BF00285186

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