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Characterization and delignification activity of a thermostable α-l-arabinofuranosidase from Bacillus stearothermophilus

Bacillus stearothermophilus L1 was isolated by enrichment culture using an alkaline extract of pulp as the carbon source at 65°C and pH 9.0. The bacterium produced extracellular xylanase and α-l-arabinofuranosidase (EC 3.2.1.55). The xylanase activity was high when the cells were grown in the presence of d-xylose, whereas the arabinofuranosidase activity was high when grown in media containing l-arabinose. The arabinofuranosidase was purified 59-fold with an 80% yield by DEAE Sephacel and Sephadex G-100 chromatography. The purified enzyme had an apparent molecular mass of 110 000 kDa and consisted of two subunits of 52 500 kDa and 57 500 kDa. Using p-nitrophenyl-α-l-arabinofuranosidase as the substrate, the enzyme had a Michaelis constant (K _m) of 2.2 × 10⁻⁴ m, maximum reaction velocity (V_max) of 11o μmol min⁻¹ mg⁻¹, temperature optimum of 70°C and pH optimum of 7.0 (50% activity at pH 8.0). The enzyme was specific for the furanoside configuration. The purified enzyme partially delignified softwood Kraft pulp. Treatment of the pulp with 38 units ml⁻¹ of α-l-arabinofuranosidase at 65°C for 2 h at pH 8.0 and 9.0 led to lignin releases of 2.3% and 2.1%, respectively. The enzyme acted synergistically with a thermophilic xylanase in the delignification process, yielding a 19.2% release of lignin.

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1. Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel

   Lea Bezalel & Eugene Rosenberg

2. Department of Food Engineering and Biotechnology, The Technio — Israel Institute of Technology, Haifa, Israel

   Yuval Shoham

1. Lea Bezalel
2. Yuval Shoham
3. Eugene Rosenberg

Correspondence to: Eugene Rosenberg

Bezalel, L., Shoham, Y. & Rosenberg, E. Characterization and delignification activity of a thermostable α-l-arabinofuranosidase from Bacillus stearothermophilus . Appl Microbiol Biotechnol 40, 57–62 (1993). https://doi.org/10.1007/BF00170429

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• Received: 11 January 1993

• Accepted: 30 April 1993

• Issue Date: October 1993

• DOI: https://doi.org/10.1007/BF00170429

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