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Showing content from https://link.springer.com/article/10.1186/s13059-018-1472-7/figures/3 below:

Figure 3 | Evolutionarily conserved partial gene duplication in the Triticeae tribe of grasses confers pathogen resistance

Proteins of H. vulgare interacting with HvARM1 and HvPUB15 in the yeast two-hybrid system (Y2H). a Full-length HvARM1 was used as bait in a Y2H screening of a cDNA library derived from Bgh-attacked barley leaves. Growth of yeast on SD-Leu/-Trp confirms the presence of both bait and prey vectors for protein expression. Growth on SD-Leu/-Trp/-His/-Ade indicates protein-protein interaction. No growth of empty bait vector + candidate prey confirms the absence of autoactivation of any prey construct for six final candidates. b Two of the six candidate protein interactors of HvARM1 also interact with full-length HvPUB15


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